Restriction map for
pF1KB7743
(Circular) (Six-base) MAPSORT of: pF1KB7743.seq Check: 8695 from: 1 to: 4424
pF1KB7743 4424 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 560 1462 560
Size: 902 3522
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 2249 2681 3428 3696 607
Size: 1642 432 747 268 1335
Fragments arranged by size:
1642 1335 747 432 268
AfeI AGC'GCT
Cuts at: 520 520
Size: 4424
AflII C'TTAA_G
Cuts at: 964 964
Size: 4424
AflIII A'CryG_T
Cuts at: 847 1776 2880 3857 847
Size: 929 1104 977 1414
Fragments arranged by size:
1414 1104 977 929
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AhdI GACnn_n'nnGTC
Cuts at: 1227 1227
Size: 4424
AleI CACnn'nnGTG
Cuts at: 694 1096 694
Size: 402 4022
AlwNI CAG_nnn'CTG
Cuts at: 408 678 765 834 877 1729 3296 408
Size: 270 87 69 43 852 1567 1536
Fragments arranged by size:
1567 1536 852 270 87 69 43
ApaI G_GGCC'C
Cuts at: 126 126
Size: 4424
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 1428 3777 4033 1428
Size: 2349 256 1819
Fragments arranged by size:
2349 1819 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 166 431 471 1351 1444 166
Size: 265 40 880 93 3146
Fragments arranged by size:
3146 880 265 93 40
BamHI G'GATC_C
Cuts at: 1449 1449
Size: 4424
BanI G'GyrC_C
Cuts at: 264 318 1440 1854 2102 2137 264
Size: 54 1122 414 248 35 2551
Fragments arranged by size:
2551 1122 414 248 54 35
BanII G_rGCy'C
Cuts at: 126 1209 1438 2468 126
Size: 1083 229 1030 2082
Fragments arranged by size:
2082 1083 1030 229
BbeI G_GCGC'C
Cuts at: 268 322 1858 2106 268
Size: 54 1536 248 2586
Fragments arranged by size:
2586 1536 248 54
BbsI GAAGACnn'nnnn_
Cuts at: 1042 1042
Size: 4424
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 899 1944 899
Size: 1045 3379
BglI GCCn_nnn'nGGC
Cuts at: 244 244
Size: 4424
BglII A'GATC_T
Cuts at: 956 1939 3976 956
Size: 983 2037 1404
Fragments arranged by size:
2037 1404 983
BlpI GC'TnA_GC
Cuts at: 535 1536 535
Size: 1001 3423
Bme1580I G_kGCm'C
Cuts at: 126 1003 2049 2142 3198 126
Size: 877 1046 93 1056 1352
Fragments arranged by size:
1352 1056 1046 877 93
BmgBI CAC'GTC
Cuts at: 848 848
Size: 4424
BmrI ACTGGGnnnn_n'
Cuts at: 329 579 1636 1717 2052 4196 329
Size: 250 1057 81 335 2144 557
Fragments arranged by size:
2144 1057 557 335 250 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1296 2791 1296
Size: 1495 2929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 547 1186 1279 175
Size: 372 639 93 3320
Fragments arranged by size:
3320 639 372 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 736 1600 2479 2971 3269 649
Size: 87 864 879 492 298 1804
Fragments arranged by size:
1804 879 864 492 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 487 910 1003 1367 4128 487
Size: 423 93 364 2761 783
Fragments arranged by size:
2761 783 423 364 93
BsaAI yAC'GTr
Cuts at: 843 1777 843
Size: 934 3490
BsaBI GATnn'nnATC
Cuts at: 6 208 781 1938 3975 6
Size: 202 573 1157 2037 455
Fragments arranged by size:
2037 1157 573 455 202
BsaHI Gr'CG_yC
Cuts at: 230 265 319 701 1855 2103 2805 4301 230
Size: 35 54 382 1154 248 702 1496 353
Fragments arranged by size:
1496 1154 702 382 353 248 54 35
BsaWI w'CCGG_w
Cuts at: 1613 2134 2812 3086 3233 3644 1613
Size: 521 678 274 147 411 2393
Fragments arranged by size:
2393 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 1021 1078 1081 1279 1312 1369 186
Size: 835 57 3 198 33 57 3241
Fragments arranged by size:
3241 835 198 57 57 33 3
BseYI C'CCAG_C
Cuts at: 678 920 1144 1849 2607 3184 3680 678
Size: 242 224 705 758 577 496 1422
Fragments arranged by size:
1422 758 705 577 496 242 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 4424
BsiEI CG_ry'CG
Cuts at: 104 360 986 1247 1966 2012 3220 104
Size: 256 626 261 719 46 1208 1308
Fragments arranged by size:
1308 1208 719 626 261 256 46
BsiHKAI G_wGCw'C
Cuts at: 1438 2216 2406 3198 1438
Size: 778 190 792 2664
Fragments arranged by size:
2664 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 126 1003 1209 1438 2049 2142 2216 2406 2468
Size: 877 206 229 611 93 74 190 62
Cuts at: 2468 3198 126
Size: 730 1352
Fragments arranged by size:
1352 877 730 611 229 206 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 3600 3600
Size: 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 302 1474 1688 1990 2371 302
Size: 1172 214 302 381 2355
Fragments arranged by size:
2355 1172 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2716 2770 4254 4419 50
Size: 2666 54 1484 165 55
Fragments arranged by size:
2666 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 644 866 2336 644
Size: 222 1470 2732
Fragments arranged by size:
2732 1470 222
BsrFI r'CCGG_y
Cuts at: 147 172 243 253 274 366 2422 2603 2812
Size: 25 71 10 21 92 2056 181 209
Cuts at: 2812 147
Size: 1759
Fragments arranged by size:
2056 1759 209 181 92 71 25 21
10
BssHII G'CGCG_C
Cuts at: 143 176 143
Size: 33 4391
BssSI C'ACGA_G
Cuts at: 1324 2695 3053 1324
Size: 1371 358 2695
Fragments arranged by size:
2695 1371 358
BstAPI GCAn_nnn'nTGC
Cuts at: 877 1801 877
Size: 924 3500
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4424
BstYI r'GATC_y
Cuts at: 1 956 1140 1449 1916 1939 2274 2520 3532
Size: 955 184 309 467 23 335 246 1012
Cuts at: 3532 3618 3630 3976 1
Size: 86 12 346 449
Fragments arranged by size:
1012 955 467 449 346 335 309 246
184 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1004 1295 1004
Size: 291 4133
BtgI C'CryG_G
Cuts at: 107 260 343 932 1211 107
Size: 153 83 589 279 3320
Fragments arranged by size:
3320 589 279 153 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2374 2555 2718 2374
Size: 181 163 4080
Fragments arranged by size:
4080 181 163
BtsI GCAGTG_nn'
Cuts at: 426 1667 426
Size: 1241 3183
ClaI AT'CG_AT
Cuts at: 986 986
Size: 4424
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DraIII CAC_nnn'GTG
Cuts at: 337 337
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 556 2130 2988 556
Size: 1574 858 1992
Fragments arranged by size:
1992 1574 858
EaeI y'GGCC_r
Cuts at: 116 357 410 1244 2009 2183 2574 2601 3946
Size: 241 53 834 765 174 391 27 1345
Cuts at: 3946 116
Size: 594
Fragments arranged by size:
1345 834 765 594 391 241 174 53
27
EagI C'GGCC_G
Cuts at: 357 1244 2009 357
Size: 887 765 2772
Fragments arranged by size:
2772 887 765
EarI CTCTTCn'nnn_
Cuts at: 307 945 1384 2447 2657 307
Size: 638 439 1063 210 2074
Fragments arranged by size:
2074 1063 638 439 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 207 722 2632 2942 3088 4234 207
Size: 515 1910 310 146 1146 397
Fragments arranged by size:
1910 1146 515 397 310 146
EcoICRI GAG'CTC
Cuts at: 1436 1436
Size: 4424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 547 607 1186 1279 2249 2681 3428 3696
Size: 372 60 579 93 970 432 747 268
Cuts at: 3696 175
Size: 903
Fragments arranged by size:
970 903 747 579 432 372 268 93
60
EcoNI CCTnn'n_nnAGG
Cuts at: 1294 1354 1294
Size: 60 4364
EcoO109I rG'GnC_Cy
Cuts at: 122 123 159 732 855 1329 1398 1563 122
Size: 1 36 573 123 474 69 165 2983
Fragments arranged by size:
2983 573 474 165 123 69 36 1
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 154 239 268 322 522 834 1858 2106 3128
Size: 85 29 54 200 312 1024 248 1022
Cuts at: 3128 4107 154
Size: 979 471
Fragments arranged by size:
1024 1022 979 471 312 248 200 85
54 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1213 1245 2266 2296 2298 2328 1213
Size: 32 1021 30 2 30 3309
Fragments arranged by size:
3309 1021 32 30 30 2
HincII GTy'rAC
Cuts at: 561 725 1463 561
Size: 164 738 3522
Fragments arranged by size:
3522 738 164
KasI G'GCGC_C
Cuts at: 264 318 1854 2102 264
Size: 54 1536 248 2586
Fragments arranged by size:
2586 1536 248 54
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 762 3095 3279 762
Size: 2333 184 1907
Fragments arranged by size:
2333 1907 184
MscI TGG'CCA
Cuts at: 2185 2185
Size: 4424
MslI CAynn'nnrTG
Cuts at: 694 1096 2413 2540 694
Size: 402 1317 127 2578
Fragments arranged by size:
2578 1317 402 127
MspA1I CmG'CkG
Cuts at: 262 345 744 765 798 1179 1236 1535 1849
Size: 83 399 21 33 381 57 299 314
Cuts at: 1849 2209 3222 3467 262
Size: 360 1013 245 1219
Fragments arranged by size:
1219 1013 399 381 360 314 299 245
83 57 33 21
NaeI GCC'GGC
Cuts at: 149 174 245 276 2605 149
Size: 25 71 31 2329 1968
Fragments arranged by size:
2329 1968 71 31 25
NarI GG'CG_CC
Cuts at: 265 319 1855 2103 265
Size: 54 1536 248 2586
Fragments arranged by size:
2586 1536 248 54
NcoI C'CATG_G
Cuts at: 107 107
Size: 4424
NgoMIV G'CCGG_C
Cuts at: 147 172 243 274 2603 147
Size: 25 71 31 2329 1968
Fragments arranged by size:
2329 1968 71 31 25
NspI r_CATG'y
Cuts at: 1267 1477 1977 2884 1267
Size: 210 500 907 2807
Fragments arranged by size:
2807 907 500 210
PasI CC'CwG_GG
Cuts at: 1030 1030
Size: 4424
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PflMI CCAn_nnn'nTGG
Cuts at: 678 834 933 678
Size: 156 99 4169
Fragments arranged by size:
4169 156 99
PfoI T'CCnGG_A
Cuts at: 450 524 1186 1256 450
Size: 74 662 70 3618
Fragments arranged by size:
3618 662 74 70
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PpuMI rG'GwC_Cy
Cuts at: 732 855 1398 732
Size: 123 543 3758
Fragments arranged by size:
3758 543 123
PspOMI G'GGCC_C
Cuts at: 122 122
Size: 4424
PspXI vC'TCGA_Gb
Cuts at: 1351 1351
Size: 4424
PstI C_TGCA'G
Cuts at: 749 882 1471 2156 749
Size: 133 589 685 3017
Fragments arranged by size:
3017 685 589 133
PvuI CG_AT'CG
Cuts at: 104 986 104
Size: 882 3542
PvuII CAG'CTG
Cuts at: 744 765 798 1849 2209 744
Size: 21 33 1051 360 2959
Fragments arranged by size:
2959 1051 360 33 21
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1438 1438
Size: 4424
SacII CC_GC'GG
Cuts at: 263 346 263
Size: 83 4341
SalI G'TCGA_C
Cuts at: 559 1461 559
Size: 902 3522
SapI GCTCTTCn'nnn_
Cuts at: 307 2447 2657 307
Size: 2140 210 2074
Fragments arranged by size:
2140 2074 210
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 745 878 1467 2152 3145 3336 33
Size: 712 133 589 685 993 191 1121
Fragments arranged by size:
1121 993 712 685 589 191 133
SfoI GGC'GCC
Cuts at: 266 320 1856 2104 266
Size: 54 1536 248 2586
Fragments arranged by size:
2586 1536 248 54
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SgrAI Cr'CCGG_yG
Cuts at: 147 366 147
Size: 219 4205
SmaI CCC'GGG
Cuts at: 473 1446 473
Size: 973 3451
SmlI C'TyrA_G
Cuts at: 628 715 964 1351 1579 2494 2986 3248 628
Size: 87 249 387 228 915 492 262 1804
Fragments arranged by size:
1804 915 492 387 262 249 228 87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 1477 1977 1477
Size: 500 3924
StyI C'CwwG_G
Cuts at: 107 113 1558 1912 107
Size: 6 1445 354 2619
Fragments arranged by size:
2619 1445 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 2007 2140 2599 2808 348
Size: 1659 133 459 209 1964
Fragments arranged by size:
1964 1659 459 209 133
TatI w'GTAC_w
Cuts at: 787 3939 787
Size: 3152 1272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 478 1125 1157 3941 3973 446
Size: 32 647 32 2784 32 897
Fragments arranged by size:
2784 897 647 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 553 2221 553
Size: 1668 2756
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 1919 522
Size: 1397 3027
XhoI C'TCGA_G
Cuts at: 1351 1351
Size: 4424
XmaI C'CCGG_G
Cuts at: 471 1444 471
Size: 973 3451
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PsrI SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XmnI ZraI