Restriction map for
pF1KE3316
(Linear) (Six-base) MAPSORT of: KIEE3316.seq Check: 2781 from: 1 to: 2634
KIEE3316.orf
With 182 enzymes: SgfI *
September 29, 2011 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2355 2634
Size: 2355 279
AccI GT'mk_AC
Cuts at: 0 736 1643 2634
Size: 736 907 991
Fragments arranged by size:
991 907 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 677 1347 1522 2634
Size: 528 149 670 175 1112
Fragments arranged by size:
1112 670 528 175 149
AfeI AGC'GCT
Cuts at: 0 535 2634
Size: 535 2099
AflIII A'CryG_T
Cuts at: 0 1898 2036 2198 2634
Size: 1898 138 162 436
Fragments arranged by size:
1898 436 162 138
AhdI GACnn_n'nnGTC
Cuts at: 0 300 2202 2634
Size: 300 1902 432
Fragments arranged by size:
1902 432 300
AleI CACnn'nnGTG
Cuts at: 0 1082 2634
Size: 1082 1552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1210 2634
Size: 1210 1424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 1831 1863 2634
Size: 223 32 1576 32 771
Fragments arranged by size:
1576 771 223 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 876 1521 1812 1974 2315 2610 2634
Size: 876 645 291 162 341 295 24
Fragments arranged by size:
876 645 341 295 291 162 24
ApaI G_GGCC'C
Cuts at: 0 573 2634
Size: 573 2061
ApaLI G'TGCA_C
Cuts at: 0 427 2634
Size: 427 2207
ApoI r'AATT_y
Cuts at: 0 1319 2634
Size: 1319 1315
AvaI C'yCGr_G
Cuts at: 0 43 120 1490 1822 2158 2634
Size: 43 77 1370 332 336 476
Fragments arranged by size:
1370 476 336 332 77 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2410 2443 2634
Size: 2410 33 191
Fragments arranged by size:
2410 191 33
BanI G'GyrC_C
Cuts at: 0 441 581 628 786 1256 1381 2154 2239
Size: 441 140 47 158 470 125 773 85
Cuts at: 2239 2634
Size: 395
Fragments arranged by size:
773 470 441 395 158 140 125 85
47
BanII G_rGCy'C
Cuts at: 0 32 573 1123 1675 2634
Size: 32 541 550 552 959
Fragments arranged by size:
959 552 550 541 32
BbeI G_GCGC'C
Cuts at: 0 445 585 1260 2634
Size: 445 140 675 1374
Fragments arranged by size:
1374 675 445 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1720 2634
Size: 1720 914
BbvCI CC'TCA_GC
Cuts at: 0 1481 2233 2634
Size: 1481 752 401
Fragments arranged by size:
1481 752 401
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 418 452 2634
Size: 418 34 2182
Fragments arranged by size:
2182 418 34
BclI T'GATC_A
Cuts at: 0 456 2216 2634
Size: 456 1760 418
Fragments arranged by size:
1760 456 418
BglI GCCn_nnn'nGGC
Cuts at: 0 1470 2152 2237 2634
Size: 1470 682 85 397
Fragments arranged by size:
1470 682 397 85
BglII A'GATC_T
Cuts at: 0 1649 2634
Size: 1649 985
BlpI GC'TnA_GC
Cuts at: 0 664 2634
Size: 664 1970
Bme1580I G_kGCm'C
Cuts at: 0 431 573 1384 2140 2634
Size: 431 142 811 756 494
Fragments arranged by size:
811 756 494 431 142
BmgBI CAC'GTC
Cuts at: 0 302 596 1728 2435 2634
Size: 302 294 1132 707 199
Fragments arranged by size:
1132 707 302 294 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1858 2337 2354 2476 2634
Size: 1858 479 17 122 158
Fragments arranged by size:
1858 479 158 122 17
Bpu10I CC'TnA_GC
Cuts at: 0 1481 1674 1940 2233 2634
Size: 1481 193 266 293 401
Fragments arranged by size:
1481 401 293 266 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 838 2634
Size: 484 354 1796
Fragments arranged by size:
1796 484 354
BsaI GGTCTCn'nnnn_
Cuts at: 0 59 138 2634
Size: 59 79 2496
Fragments arranged by size:
2496 79 59
BsaHI Gr'CG_yC
Cuts at: 0 442 582 1257 1855 2634
Size: 442 140 675 598 779
Fragments arranged by size:
779 675 598 442 140
BsaWI w'CCGG_w
Cuts at: 0 334 1039 1709 1784 2634
Size: 334 705 670 75 850
Fragments arranged by size:
850 705 670 334 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1831 1861 2634
Size: 1831 30 773
Fragments arranged by size:
1831 773 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 47 708 841 994 1102 1105 2634
Size: 47 661 133 153 108 3 1529
Fragments arranged by size:
1529 661 153 133 108 47 3
BseYI C'CCAG_C
Cuts at: 0 1496 1549 1668 2507 2634
Size: 1496 53 119 839 127
Fragments arranged by size:
1496 839 127 119 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 2634
Size: 633 2001
BsiHKAI G_wGCw'C
Cuts at: 0 431 1123 2634
Size: 431 692 1511
Fragments arranged by size:
1511 692 431
BsmI GAATG_Cn'
Cuts at: 0 1074 2634
Size: 1074 1560
BsmBI CGTCTCn'nnnn_
Cuts at: 0 602 1846 2634
Size: 602 1244 788
Fragments arranged by size:
1244 788 602
Bsp1286I G_dGCh'C
Cuts at: 0 32 431 573 1123 1384 1675 2140 2634
Size: 32 399 142 550 261 291 465 494
Fragments arranged by size:
550 494 465 399 291 261 142 32
BspEI T'CCGG_A
Cuts at: 0 334 1784 2634
Size: 334 1450 850
Fragments arranged by size:
1450 850 334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2355 2366 2634
Size: 2355 11 268
Fragments arranged by size:
2355 268 11
BsrBI CCG'CTC
Cuts at: 0 2126 2189 2634
Size: 2126 63 445
Fragments arranged by size:
2126 445 63
BsrDI GCAATG_nn'
Cuts at: 0 886 1631 2634
Size: 886 745 1003
Fragments arranged by size:
1003 886 745
BsrFI r'CCGG_y
Cuts at: 0 1931 2142 2634
Size: 1931 211 492
Fragments arranged by size:
1931 492 211
BssHII G'CGCG_C
Cuts at: 0 2503 2634
Size: 2503 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 876 890 1161 1812 2535 2634
Size: 324 552 14 271 651 723 99
Fragments arranged by size:
723 651 552 324 271 99 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 600 756 2634
Size: 600 156 1878
Fragments arranged by size:
1878 600 156
BstYI r'GATC_y
Cuts at: 0 110 275 404 1613 1649 1970 2279 2565
Size: 110 165 129 1209 36 321 309 286
Cuts at: 2565 2634
Size: 69
Fragments arranged by size:
1209 321 309 286 165 129 110 69
36
BstZ17I GTA'TAC
Cuts at: 0 737 2634
Size: 737 1897
Bsu36I CC'TnA_GG
Cuts at: 0 2066 2634
Size: 2066 568
BtgI C'CryG_G
Cuts at: 0 2555 2634
Size: 2555 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 521 2634
Size: 521 2113
BtsI GCAGTG_nn'
Cuts at: 0 459 1392 1409 2634
Size: 459 933 17 1225
Fragments arranged by size:
1225 933 459 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 546 581 2634
Size: 546 35 2053
Fragments arranged by size:
2053 546 35
DraIII CAC_nnn'GTG
Cuts at: 0 618 2634
Size: 618 2016
DrdI GACnn_nn'nnGTC
Cuts at: 0 1042 2634
Size: 1042 1592
EaeI y'GGCC_r
Cuts at: 0 2 305 383 602 609 1796 2306 2634
Size: 2 303 78 219 7 1187 510 328
Fragments arranged by size:
1187 510 328 303 219 78 7 2
EarI CTCTTCn'nnn_
Cuts at: 0 481 653 717 726 868 1288 2634
Size: 481 172 64 9 142 420 1346
Fragments arranged by size:
1346 481 420 172 142 64 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 50 397 1341 1501 2110 2629 2634
Size: 50 347 944 160 609 519 5
Fragments arranged by size:
944 609 519 347 160 50 5
EcoICRI GAG'CTC
Cuts at: 0 1121 2634
Size: 1121 1513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 677 1347 1522 1858 2337 2354 2476
Size: 528 149 670 175 336 479 17 122
Cuts at: 2476 2634
Size: 158
Fragments arranged by size:
670 528 479 336 175 158 149 122
17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 946 2362 2634
Size: 361 585 1416 272
Fragments arranged by size:
1416 585 361 272
EcoO109I rG'GnC_Cy
Cuts at: 0 495 569 858 873 951 1518 2576 2634
Size: 495 74 289 15 78 567 1058 58
Fragments arranged by size:
1058 567 495 289 78 74 58 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 879 2634
Size: 879 1755
FspI TGC'GCA
Cuts at: 0 2377 2634
Size: 2377 257
HaeII r_GCGC'y
Cuts at: 0 445 537 585 1016 1260 1556 1947 2634
Size: 445 92 48 431 244 296 391 687
Fragments arranged by size:
687 445 431 391 296 244 92 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 286 318 2188 2220 2634
Size: 70 32 184 32 1870 32 414
Fragments arranged by size:
1870 414 184 70 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1580 2634
Size: 1580 1054
KasI G'GCGC_C
Cuts at: 0 441 581 1256 2634
Size: 441 140 675 1378
Fragments arranged by size:
1378 675 441 140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1628 1883 2634
Size: 1628 255 751
Fragments arranged by size:
1628 751 255
MscI TGG'CCA
Cuts at: 0 4 604 611 1798 2634
Size: 4 600 7 1187 836
Fragments arranged by size:
1187 836 600 7 4
MslI CAynn'nnrTG
Cuts at: 0 1082 1597 2492 2634
Size: 1082 515 895 142
Fragments arranged by size:
1082 895 515 142
MspA1I CmG'CkG
Cuts at: 0 213 321 1326 2237 2332 2535 2634
Size: 213 108 1005 911 95 203 99
Fragments arranged by size:
1005 911 213 203 108 99 95
NaeI GCC'GGC
Cuts at: 0 1933 2144 2634
Size: 1933 211 490
Fragments arranged by size:
1933 490 211
NarI GG'CG_CC
Cuts at: 0 442 582 1257 2634
Size: 442 140 675 1377
Fragments arranged by size:
1377 675 442 140
NgoMIV G'CCGG_C
Cuts at: 0 1931 2142 2634
Size: 1931 211 492
Fragments arranged by size:
1931 492 211
NspI r_CATG'y
Cuts at: 0 1848 1902 2202 2604 2634
Size: 1848 54 300 402 30
Fragments arranged by size:
1848 402 300 54 30
PasI CC'CwG_GG
Cuts at: 0 1125 2634
Size: 1125 1509
PciI A'CATG_T
Cuts at: 0 1898 2198 2634
Size: 1898 300 436
Fragments arranged by size:
1898 436 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 1974 2315 2634
Size: 1974 341 319
Fragments arranged by size:
1974 341 319
PfoI T'CCnGG_A
Cuts at: 0 344 2634
Size: 344 2290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1831 1863 2101 2133 2634
Size: 1831 32 238 32 501
Fragments arranged by size:
1831 501 238 32 32
PspOMI G'GGCC_C
Cuts at: 0 569 2634
Size: 569 2065
PstI C_TGCA'G
Cuts at: 0 86 320 1808 2366 2634
Size: 86 234 1488 558 268
Fragments arranged by size:
1488 558 268 234 86
PvuII CAG'CTG
Cuts at: 0 213 321 2332 2634
Size: 213 108 2011 302
Fragments arranged by size:
2011 302 213 108
SacI G_AGCT'C
Cuts at: 0 1123 2634
Size: 1123 1511
SapI GCTCTTCn'nnn_
Cuts at: 0 481 653 1288 2634
Size: 481 172 635 1346
Fragments arranged by size:
1346 635 481 172
SexAI A'CCwGG_T
Cuts at: 0 356 2634
Size: 356 2278
SfcI C'TryA_G
Cuts at: 0 82 316 561 1804 2362 2385 2634
Size: 82 234 245 1243 558 23 249
Fragments arranged by size:
1243 558 249 245 234 82 23
SfoI GGC'GCC
Cuts at: 0 443 583 1258 2634
Size: 443 140 675 1376
Fragments arranged by size:
1376 675 443 140
SmaI CCC'GGG
Cuts at: 0 45 122 1492 1824 2634
Size: 45 77 1370 332 810
Fragments arranged by size:
1370 810 332 77 45
SmlI C'TyrA_G
Cuts at: 0 499 853 2634
Size: 499 354 1781
Fragments arranged by size:
1781 499 354
StyI C'CwwG_G
Cuts at: 0 776 2634
Size: 776 1858
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1197 1563 1981 2634
Size: 1197 366 418 653
Fragments arranged by size:
1197 653 418 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1255 1287 1443 1475 2634
Size: 1255 32 156 32 1159
Fragments arranged by size:
1255 1159 156 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1428 2329 2634
Size: 1428 901 305
Fragments arranged by size:
1428 901 305
XmaI C'CCGG_G
Cuts at: 0 43 120 1490 1822 2634
Size: 43 77 1370 332 812
Fragments arranged by size:
1370 812 332 77 43
XmnI GAAnn'nnTTC
Cuts at: 0 2116 2634
Size: 2116 518
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PpiI PspOMI PstI PvuII SacI
SapI SexAI SfcI SfoI SmaI SmlI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AscI AseI AsiSI
AvrII BamHI BciVI BfrBI BmrI BmtI BplI BsaAI
BsaBI BsiEI BsiWI BspHI BsrGI BssSI BstBI BstEII
ClaI DraI EagI EcoRI EcoRV FseI FspAI HincII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI