Restriction map for pF1KE5416
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrGI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HgaI        HindIII     HpaI        
KpnI        Ksp632I     MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tru9I       
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         175  GACGTC               GACGT^C
AccI          502  GTCTAC               GT^[AC][GT]AC
AcsI          589  GAATTC               [GA]^AATT[CT]
AfaI          458  GTAC                 GT^AC
AlwNI          85  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          949  GGGCCC               GGGCC^C
ApoI          589  GAATTC               [GA]^AATT[CT]
AspI          552  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          903  TGGCCA               TGG^CCA
BbeI          895  GGCGCC               GGCGC^C
BbsI          147  GAAGAC               GAAGAC(2/6)
BciVI         933  GTATCC               GTATCC(6/5)
BglI          190  GCCGTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         147  GAAGAC               GAAGAC(2/6)
BsiEI         470  CGGTCG               CG[GA][CT]^CG
BsrDI         318  GCAATG               GCAATG(2/0)
BsrFI         468  GCCGGT               [GA]^CCGG[CT]
BssHII        161  GCGCGC               G^CGCGC
BssSI         856  CACGAG               CACGAG(-5/-1)
BstYI         282  GGATCT               [GA]^GATC[CT]
Cfr10I        468  GCCGGT               [GA]^CCGG[CT]
DrdI          520  GACGGCAACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         937  CCTGCAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         589  GAATTC               G^AATTC
HincII        885  GTTGAC               GT[CT]^[GA]AC
HindII        885  GTTGAC               GT[CT]^[GA]AC
KasI          895  GGCGCC               G^GCGCC
KspI          273  CCGCGG               CCGC^GG
MflI          282  GGATCT               [GA]^GATC[CT]
MluNI         903  TGGCCA               TGG^CCA
MscI          903  TGGCCA               TGG^CCA
MslI          738  CACGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          895  GGCGCC               GG^CGCC
PflFI         552  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          32  CCATGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         652  GGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          458  GTAC                 GT^AC
SacII         273  CCGCGG               CCGC^GG
SfaNI         128  GATGC                GCATC(5/9)
SfoI          895  GGCGCC               GGC^GCC
SmaI          866  CCCGGG               CCC^GGG
StuI          860  AGGCCT               AGG^CCT
TfiI          701  GAATC                G^A[AT]TC
Tth111I       552  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         32  CCATGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          986  CCACAGCTGGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         282  GGATCT               [GA]^GATC[CT]
XmaCI         866  CCCGGG               C^CCGGG
XmaI          866  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI          175  GACGTC               G[GA]^CG[CT]C
              895  GGCGCC              
AlwI          282  GGATC                GGATC(4/5)
              330  GGATC               
AvaI          599  CTCGGG               C^[CT]CG[GA]G
              866  CCCGGG              
BanI           22  GGTGCC               G^G[CT][GA]CC
              895  GGCGCC              
BsaHI         175  GACGTC               G[GA]^CG[CT]C
              895  GGCGCC              
BsaI          205  GAGACC               GGTCTC(1/5)
              789  GAGACC              
BseRI          77  GAGGAG               GAGGAG(10/8)
              874  GAGGAG              
BsmFI         252  GGGAC                GGGAC(10/14)
              654  GTCCC               
BsoBI         599  CTCGGG               C^[CT]CG[GA]G
              866  CCCGGG              
BspMI          19  GCAGGT               ACCTGC(4/8)
              379  GCAGGT              
BstXI         404  CCAACGGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              737  CCACGACTGTGG        
EaeI          903  TGGCCA               [CT]^GGCC[GA]
             1013  TGGCCG              
Hin1I         175  GACGTC               G[GA]^CG[CT]C
              895  GGCGCC              
MaeII         176  ACGT                 A^CGT
              527  ACGT                
MboII         147  GAAGA                GAAGA(8/7)
              285  TCTTC               
PleI          254  GACTC                GAGTC(4/5)
              834  GACTC               
PstI           44  CTGCAG               CTGCA^G
              313  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsgI        BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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