Restriction map for pF1KE5416
 (Linear) (Six-base) MAPSORT of: KIEE5416.seq  Check: 136  from: 1  to: 1038



KIEE5416.orf

 With 182 enzymes: SgfI * 

                        September 8, 2016 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      10    1038 
   Size:         10    1028
 
AatII G_ACGT'C

Cuts at:      0     179    1038 
   Size:        179     859
 
AccI GT'mk_AC

Cuts at:      0     503    1038 
   Size:        503     535
 
AleI CACnn'nnGTG

Cuts at:      0     742    1038 
   Size:        742     296
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     953    1038 
   Size:        953      85
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90    1038 
   Size:         90     948
 
ApaI G_GGCC'C

Cuts at:      0     953    1038 
   Size:        953      85
 
ApoI r'AATT_y

Cuts at:      0     589    1038 
   Size:        589     449
 
AvaI C'yCGr_G

Cuts at:      0     599     866    1038 
   Size:        599     267     172

  Fragments arranged by size: 

                 599     267     172
 
BanI G'GyrC_C

Cuts at:      0      22     895    1038 
   Size:         22     873     143

  Fragments arranged by size: 

                 873     143      22
 
BanII G_rGCy'C

Cuts at:      0     737     881     953    1038 
   Size:        737     144      72      85

  Fragments arranged by size: 

                 737     144      85      72
 
BbeI G_GCGC'C

Cuts at:      0     899    1038 
   Size:        899     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     154    1038 
   Size:        154     884
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     944    1038 
   Size:        944      94
 
BglI GCCn_nnn'nGGC

Cuts at:      0     196    1038 
   Size:        196     842
 
Bme1580I G_kGCm'C

Cuts at:      0      27     953    1038 
   Size:         27     926      85

  Fragments arranged by size: 

                 926      85      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     778    1038 
   Size:        778     260
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     713     911    1038 
   Size:        112     601     198     127

  Fragments arranged by size: 

                 601     198     127     112
 
Bpu10I CC'TnA_GC

Cuts at:      0     326     436    1038 
   Size:        326     110     602

  Fragments arranged by size: 

                 602     326     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     199     783    1038 
   Size:        199     584     255

  Fragments arranged by size: 

                 584     255     199
 
BsaHI Gr'CG_yC

Cuts at:      0     176     896    1038 
   Size:        176     720     142

  Fragments arranged by size: 

                 720     176     142
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145     847     868     877     898    1038 
   Size:        115      30     702      21       9      21     140

  Fragments arranged by size: 

                 702     140     115      30      21      21       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      92     889    1038 
   Size:         92     797     149

  Fragments arranged by size: 

                 797     149      92
 
BseYI C'CCAG_C

Cuts at:      0     953    1038 
   Size:        953      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     165     579    1038 
   Size:        130      35     414     459

  Fragments arranged by size: 

                 459     414     130      35
 
BsiEI CG_ry'CG

Cuts at:      0     473    1038 
   Size:        473     565
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     737     881     953    1038 
   Size:         27     710     144      72      85

  Fragments arranged by size: 

                 710     144      85      72      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      10     370    1038 
   Size:         10     360     668

  Fragments arranged by size: 

                 668     360      10
 
BsrDI GCAATG_nn'

Cuts at:      0     325    1038 
   Size:        325     713
 
BsrFI r'CCGG_y

Cuts at:      0     468    1038 
   Size:        468     570
 
BssHII G'CGCG_C

Cuts at:      0     161    1038 
   Size:        161     877
 
BssSI C'ACGA_G

Cuts at:      0     856    1038 
   Size:        856     182
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     411     744    1038 
   Size:        411     333     294

  Fragments arranged by size: 

                 411     333     294
 
BstYI r'GATC_y

Cuts at:      0     282    1038 
   Size:        282     756
 
BtgI C'CryG_G

Cuts at:      0     191     213     273    1016    1038 
   Size:        191      22      60     743      22

  Fragments arranged by size: 

                 743     191      60      22      22
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     446    1038 
   Size:        446     592
 
BtsI GCAGTG_nn'

Cuts at:      0     239    1038 
   Size:        239     799
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     526    1038 
   Size:        526     512
 
EaeI y'GGCC_r

Cuts at:      0     903    1013    1038 
   Size:        903     110      25

  Fragments arranged by size: 

                 903     110      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     713     911    1038 
   Size:        112     601     198     127

  Fragments arranged by size: 

                 601     198     127     112
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     941    1038 
   Size:        941      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     653     949     950    1038 
   Size:         87     566     296       1      88

  Fragments arranged by size: 

                 566     296      88      87       1
 
EcoRI G'AATT_C

Cuts at:      0     589    1038 
   Size:        589     449
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     531     705    1038 
   Size:        531     174     333

  Fragments arranged by size: 

                 531     333     174
 
HaeII r_GCGC'y

Cuts at:      0     370     823     899    1038 
   Size:        370     453      76     139

  Fragments arranged by size: 

                 453     370     139      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147    1038 
   Size:        115      32     891

  Fragments arranged by size: 

                 891     115      32
 
HincII GTy'rAC

Cuts at:      0     887    1038 
   Size:        887     151
 
KasI G'GCGC_C

Cuts at:      0     895    1038 
   Size:        895     143
 
MscI TGG'CCA

Cuts at:      0     905    1038 
   Size:        905     133
 
MslI CAynn'nnrTG

Cuts at:      0     742    1038 
   Size:        742     296
 
MspA1I CmG'CkG

Cuts at:      0     104     275     312     425     659     901     991    1038 
   Size:        104     171      37     113     234     242      90      47

  Fragments arranged by size: 

                 242     234     171     113     104      90      47      37
            
 
NarI GG'CG_CC

Cuts at:      0     896    1038 
   Size:        896     142
 
PasI CC'CwG_GG

Cuts at:      0     619    1038 
   Size:        619     419
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      38    1038 
   Size:         38    1000
 
PfoI T'CCnGG_A

Cuts at:      0     581    1038 
   Size:        581     457
 
PpuMI rG'GwC_Cy

Cuts at:      0     653    1038 
   Size:        653     385
 
PspOMI G'GGCC_C

Cuts at:      0     949    1038 
   Size:        949      89
 
PstI C_TGCA'G

Cuts at:      0      48     317    1038 
   Size:         48     269     721

  Fragments arranged by size: 

                 721     269      48
 
PvuII CAG'CTG

Cuts at:      0     104     312     425     991    1038 
   Size:        104     208     113     566      47

  Fragments arranged by size: 

                 566     208     113     104      47
 
SacII CC_GC'GG

Cuts at:      0     276    1038 
   Size:        276     762
 
SanDI GG'GwC_CC

Cuts at:      0     653    1038 
   Size:        653     385
 
SfcI C'TryA_G

Cuts at:      0      44      99     313     420    1038 
   Size:         44      55     214     107     618

  Fragments arranged by size: 

                 618     214     107      55      44
 
SfoI GGC'GCC

Cuts at:      0     897    1038 
   Size:        897     141
 
SmaI CCC'GGG

Cuts at:      0     868    1038 
   Size:        868     170
 
StuI AGG'CCT

Cuts at:      0     862    1038 
   Size:        862     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       6      38     698     730     847     879    1038 
   Size:          6      32     660      32     117      32     159

  Fragments arranged by size: 

                 660     159     117      32      32      32       6
 
Tth111I GACn'n_nGTC

Cuts at:      0     555    1038 
   Size:        555     483
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     993    1038 
   Size:        993      45
 
XmaI C'CCGG_G

Cuts at:      0     866    1038 
   Size:        866     172
 
ZraI GAC'GTC

Cuts at:      0     177    1038 
   Size:        177     861
 

 Enzymes that do cut:

     AarI    AatII     AccI     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BciVI     BglI Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI Bsp1286I    BspMI    BsrDI    BsrFI   BssHII    BssSI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KasI
     MscI     MslI   MspA1I     NarI     PasI    PflMI     PfoI    PpuMI
   PspOMI     PstI    PvuII    SacII    SanDI     SfcI     SfoI     SmaI
     StuI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TatI     XbaI     XhoI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com