Restriction map for
pF1KE2345
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbeI
BbsI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI Tsp45I Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 859 GACGCC G[GA]^CG[CT]C
AflIII 656 ACGTGT A^C[GA][CT]GT
Alw44I 319 GTGCAC G^TGCAC
ApaLI 319 GTGCAC G^TGCAC
AspHI 319 GTGCAC G[AT]GC[AT]^C
AvaI 369 CCCGAG C^[CT]CG[GA]G
BamHI 3 GGATCC G^GATCC
BbrPI 871 CACGTG CAC^GTG
BglI 787 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 871 CACGTG [CT]AC^GT[GA]
BsaHI 859 GACGCC G[GA]^CG[CT]C
BsiEI 282 CGACCG CG[GA][CT]^CG
BsiHKAI 319 GTGCAC G[AT]GC[AT]^C
BsoBI 369 CCCGAG C^[CT]CG[GA]G
Bsp1407I 338 TGTACA T^GTACA
BspMI 911 ACCTGC ACCTGC(4/8)
BsrBI 544 CCGCTC CCGCTC(-3/-3)
BsrGI 338 TGTACA T^GTACA
BstAPI 1010 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 893 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 681 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 38 GGCGCT [GA]GCGC^[CT]
Hin1I 859 GACGCC G[GA]^CG[CT]C
MseI 212 TTAA T^TAA
MslI 867 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 440 CCATGG C^CATGG
PmaCI 871 CACGTG CAC^GTG
PmlI 871 CACGTG CAC^GTG
SspBI 338 TGTACA T^GTACA
TaqI 737 TCGA T^CGA
TfiI 1036 GAATC G^A[AT]TC
Tru9I 212 TTAA T^TAA
TthHB8I 737 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 908 GGTACC G^GTACC
1043 GGTACC
AccII 309 CGCG CG^CG
1054 CGCG
ApaI 12 GGGCCC GGGCC^C
819 GGGCCC
Asp718I 908 GGTACC G^GTACC
1043 GGTACC
AspI 307 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
705 GACGCTGTC
BciVI 448 GGATAC GTATCC(6/5)
294 GTATCC
BpmI 511 CTGGAG CTGGAG(16/14)
790 CTGGAG
BsmBI 703 GAGACG CGTCTC(1/5)
967 GAGACG
BsrI 593 ACTGG ACTGG(1/-1)
347 CCAGT
BstF5I 180 CATCC GGATG(2/0)
867 CATCC
BstUI 309 CGCG CG^CG
1054 CGCG
BstYI 3 GGATCC [GA]^GATC[CT]
392 GGATCT
CfoI 39 GCGC GCG^C
618 GCGC
DdeI 240 CTCAG C^T[ACGT]AG
946 CTGAG
EcoT14I 440 CCATGG C^C[AT][AT]GG
893 CCAAGG
FokI 180 CATCC GGATG(9/13)
867 CATCC
HhaI 39 GCGC GCG^C
618 GCGC
HinP1I 39 GCGC G^CGC
618 GCGC
KpnI 908 GGTACC GGTAC^C
1043 GGTACC
MboII 89 TCTTC GAAGA(8/7)
491 TCTTC
MflI 3 GGATCC [GA]^GATC[CT]
392 GGATCT
MvnI 309 CGCG CG^CG
1054 CGCG
PflFI 307 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
705 GACGCTGTC
PleI 481 GACTC GAGTC(4/5)
409 GAGTC
SbfI 633 CCTGCAGG CCTGCA^GG
912 CCTGCAGG
SmlI 110 CTCAAG C^T[CT][GA]AG
646 CTCAAG
Sse8387I 633 CCTGCAGG CCTGCA^GG
912 CCTGCAGG
StuI 794 AGGCCT AGG^CCT
1013 AGGCCT
StyI 440 CCATGG C^C[AT][AT]GG
893 CCAAGG
Tth111I 307 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
705 GACGCTGTC
XhoII 3 GGATCC [GA]^GATC[CT]
392 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstNI Cac8I
Cfr13I DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeII MboI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
TseI TspRI XcmI