Restriction map for pF1KB0459
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     AviII       
AvrII       BamHI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BsaBI       BsaWI       
BseAI       BsiWI       BsmI        BspEI       BspLU11I    
BsrDI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CpoI        DraI        DrdI        Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MunI        NcoI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        TfiI        Tru9I       Tsp509I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII         496  GACGTC               GACGT^C
AlwI          885  GATCC                GGATC(4/5)
ApaI          143  GGGCCC               GGGCC^C
BbeI         1176  GGCGCC               GGCGC^C
BbsI         1252  GTCTTC               GAAGAC(2/6)
BfaI         1144  CTAG                 C^TAG
BglII         380  AGATCT               A^GATCT
BpuAI        1252  GTCTTC               GAAGAC(2/6)
Bsp1407I      902  TGTACA               T^GTACA
BspDI         541  ATCGAT               AT^CGAT
BsrGI         902  TGTACA               T^GTACA
BssHII        451  GCGCGC               G^CGCGC
BstEII        225  GGTCACC              G^GT[ACGT]ACC
BstPI         225  GGTCACC              G^GT[ACGT]ACC
BstXI         276  CCACACCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         380  AGATCT               [GA]^GATC[CT]
ClaI          541  ATCGAT               AT^CGAT
DraIII        153  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           26  GAAGAG               CTCTTC(1/4)
EcoO65I       225  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1100  CCAAGG               C^C[AT][AT]GG
HindIII      1042  AAGCTT               A^AGCTT
KasI         1176  GGCGCC               G^GCGCC
Ksp632I        26  GAAGAG               CTCTTC(1/4)
MaeI         1144  CTAG                 C^TAG
MflI          380  AGATCT               [GA]^GATC[CT]
NarI         1176  GGCGCC               GG^CGCC
NdeI         1259  CATATG               CA^TATG
PshAI         831  GACGGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI          750  CCTGCAGG             CCTGCA^GG
SfoI         1176  GGCGCC               GGC^GCC
SmlI         1149  CTCAAG               C^T[CT][GA]AG
Sse8387I      750  CCTGCAGG             CCTGCA^GG
SspBI         902  TGTACA               T^GTACA
StyI         1100  CCAAGG               C^C[AT][AT]GG
XcmI          130  CCAACGAGGCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         380  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        260  GGTACC               G^GTACC
             1322  GGTACC              
AhdI           51  GACCCGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              954  GACCCGGGGTC         
Alw44I       1048  GTGCAC               G^TGCAC
             1360  GTGCAC              
ApaLI        1048  GTGCAC               G^TGCAC
             1360  GTGCAC              
Asp718I       260  GGTACC               G^GTACC
             1322  GGTACC              
AspEI          51  GACCCGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              954  GACCCGGGGTC         
BbrPI         919  CACGTG               CAC^GTG
             1006  CACGTG              
BglI          129  GCCAACGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              455  GCCACCCCGGC         
BlpI          351  GCTCAGC              GC^T[ACGT]AGC
              982  GCTCAGC             
Bpu1102I      351  GCTCAGC              GC^T[ACGT]AGC
              982  GCTCAGC             
BsaAI         919  CACGTG               [CT]AC^GT[GA]
             1006  CACGTG              
BsaI          627  GAGACC               GGTCTC(1/5)
             1080  GGTCTC              
BseRI         661  GAGGAG               GAGGAG(10/8)
              664  GAGGAG              
BsiEI         656  CGGCCG               CG[GA][CT]^CG
              833  CGGCCG              
BspHI         839  TCATGA               T^CATGA
             1370  TCATGA              
BspMI         695  ACCTGC               ACCTGC(4/8)
             1456  GCAGGT              
BsrFI        1154  GCCGGC               [GA]^CCGG[CT]
             1403  GCCGGC              
BssSI         856  CACGAG               CACGAG(-5/-1)
              970  CTCGTG              
CelII         351  GCTCAGC              GC^T[ACGT]AGC
              982  GCTCAGC             
Cfr10I       1154  GCCGGC               [GA]^CCGG[CT]
             1403  GCCGGC              
EagI          656  CGGCCG               C^GGCCG
              833  CGGCCG              
Eam1105I       51  GACCCGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              954  GACCCGGGGTC         
EclXI         656  CGGCCG               C^GGCCG
              833  CGGCCG              
Eco52I        656  CGGCCG               C^GGCCG
              833  CGGCCG              
EcoNI          40  CCTCGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              888  CCTGCACCAGG         
HinfI         445  GAGTC                G^A[ACGT]TC
             1147  GACTC               
KpnI          260  GGTACC               GGTAC^C
             1322  GGTACC              
NaeI         1154  GCCGGC               GCC^GGC
             1403  GCCGGC              
NgoMIV       1154  GCCGGC               G^CCGGC
             1403  GCCGGC              
PleI         1147  GACTC                GAGTC(4/5)
              445  GAGTC               
PmaCI         919  CACGTG               CAC^GTG
             1006  CACGTG              
PmlI          919  CACGTG               CAC^GTG
             1006  CACGTG              
PpuMI          49  GGGACCC              [GA]G^G[AT]CC[CT]
             1088  AGGACCT             
RcaI          839  TCATGA               T^CATGA
             1370  TCATGA              
SfaNI         126  GATGC                GCATC(5/9)
              849  GATGC               
StuI          239  AGGCCT               AGG^CCT
              815  AGGCCT              
TaqI          542  TCGA                 T^CGA
              963  TCGA                
TthHB8I       542  TCGA                 T^CGA
              963  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflFI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfcI        SmaI        TseI        
Tsp45I      TspRI       Tth111I     XmaCI       XmaI        
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