Restriction map for pF1KB0459
 (Linear) (Six-base) MAPSORT of: KIBB0459.seq  Check: 9041  from: 1  to: 1524



KIBB0459.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1447    1524 
   Size:       1447      77
 
AatII G_ACGT'C

Cuts at:      0     500    1524 
   Size:        500    1024
 
Acc65I G'GTAC_C

Cuts at:      0     260    1322    1524 
   Size:        260    1062     202

  Fragments arranged by size: 

                1062     260     202
 
AhdI GACnn_n'nnGTC

Cuts at:      0      56     959    1524 
   Size:         56     903     565

  Fragments arranged by size: 

                 903     565      56
 
AleI CACnn'nnGTG

Cuts at:      0     281    1524 
   Size:        281    1243
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     415     447    1524 
   Size:        415      32    1077

  Fragments arranged by size: 

                1077     415      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      72     408     900     990    1382    1524 
   Size:         72     336     492      90     392     142

  Fragments arranged by size: 

                 492     392     336     142      90      72
 
ApaI G_GGCC'C

Cuts at:      0     147    1524 
   Size:        147    1377
 
ApaLI G'TGCA_C

Cuts at:      0    1048    1360    1524 
   Size:       1048     312     164

  Fragments arranged by size: 

                1048     312     164
 
AvaI C'yCGr_G

Cuts at:      0     147     384     418     956    1180    1339    1439    1524 
   Size:        147     237      34     538     224     159     100      85

  Fragments arranged by size: 

                 538     237     224     159     147     100      85      34
            
 
BanI G'GyrC_C

Cuts at:      0     101     151     260    1176    1322    1459    1524 
   Size:        101      50     109     916     146     137      65

  Fragments arranged by size: 

                 916     146     137     109     101      65      50
 
BanII G_rGCy'C

Cuts at:      0      82     147     969    1272    1397    1523    1524 
   Size:         82      65     822     303     125     126       1

  Fragments arranged by size: 

                 822     303     126     125      82      65       1
 
BbeI G_GCGC'C

Cuts at:      0    1180    1524 
   Size:       1180     344
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1245    1524 
   Size:       1245     279
 
BglI GCCn_nnn'nGGC

Cuts at:      0     135     461    1524 
   Size:        135     326    1063

  Fragments arranged by size: 

                1063     326     135
 
BglII A'GATC_T

Cuts at:      0     380    1524 
   Size:        380    1144
 
BlpI GC'TnA_GC

Cuts at:      0     352     983    1524 
   Size:        352     631     541

  Fragments arranged by size: 

                 631     541     352
 
Bme1580I G_kGCm'C

Cuts at:      0     147     154    1052    1364    1464    1524 
   Size:        147       7     898     312     100      60

  Fragments arranged by size: 

                 898     312     147     100      60       7
 
BmgBI CAC'GTC

Cuts at:      0     222    1365    1524 
   Size:        222    1143     159

  Fragments arranged by size: 

                1143     222     159
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     150    1524 
   Size:        150    1374
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     351    1272    1524 
   Size:        351     921     252

  Fragments arranged by size: 

                 921     351     252
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256    1051    1245    1524 
   Size:        256     795     194     279

  Fragments arranged by size: 

                 795     279     256     194
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1134    1524 
   Size:       1134     390
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     621    1086    1524 
   Size:        621     465     438

  Fragments arranged by size: 

                 621     465     438
 
BsaAI yAC'GTr

Cuts at:      0     921    1008    1524 
   Size:        921      87     516

  Fragments arranged by size: 

                 921     516      87
 
BsaHI Gr'CG_yC

Cuts at:      0     497    1177    1487    1524 
   Size:        497     680     310      37

  Fragments arranged by size: 

                 680     497     310      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1306    1336    1524 
   Size:       1306      30     188

  Fragments arranged by size: 

                1306     188      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     676     679    1524 
   Size:        676       3     845

  Fragments arranged by size: 

                 845     676       3
 
BseYI C'CCAG_C

Cuts at:      0     140     307    1382    1464    1524 
   Size:        140     167    1075      82      60

  Fragments arranged by size: 

                1075     167     140      82      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502     597     874     988    1475    1524 
   Size:        502      95     277     114     487      49

  Fragments arranged by size: 

                 502     487     277     114      95      49
 
BsiEI CG_ry'CG

Cuts at:      0     659     836    1524 
   Size:        659     177     688

  Fragments arranged by size: 

                 688     659     177
 
BsiHKAI G_wGCw'C

Cuts at:      0     179     720     920    1052    1364    1524 
   Size:        179     541     200     132     312     160

  Fragments arranged by size: 

                 541     312     200     179     160     132
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     120     823     877    1479    1524 
   Size:        120     703      54     602      45

  Fragments arranged by size: 

                 703     602     120      54      45
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     147     154     179     720     920     969    1052 
   Size:         82      65       7      25     541     200      49      83

Cuts at:   1052    1272    1364    1397    1464    1523    1524 
   Size:        220      92      33      67      59       1

  Fragments arranged by size: 

                 541     220     200      92      83      82      67      65
                  59      49      33      25       7       1
 
BspHI T'CATG_A

Cuts at:      0     839    1370    1524 
   Size:        839     531     154

  Fragments arranged by size: 

                 839     531     154
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     704    1447    1524 
   Size:        704     743      77

  Fragments arranged by size: 

                 743     704      77
 
BsrBI CCG'CTC

Cuts at:      0     324     982    1134    1524 
   Size:        324     658     152     390

  Fragments arranged by size: 

                 658     390     324     152
 
BsrFI r'CCGG_y

Cuts at:      0    1154    1403    1524 
   Size:       1154     249     121

  Fragments arranged by size: 

                1154     249     121
 
BsrGI T'GTAC_A

Cuts at:      0     902    1524 
   Size:        902     622
 
BssHII G'CGCG_C

Cuts at:      0     451    1524 
   Size:        451    1073
 
BssSI C'ACGA_G

Cuts at:      0     856     970    1524 
   Size:        856     114     554

  Fragments arranged by size: 

                 856     554     114
 
BstEII G'GTnAC_C

Cuts at:      0     225    1524 
   Size:        225    1299
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     283    1524 
   Size:        283    1241
 
BstYI r'GATC_y

Cuts at:      0     380    1524 
   Size:        380    1144
 
BtgI C'CryG_G

Cuts at:      0      54     364     372    1310    1524 
   Size:         54     310       8     938     214

  Fragments arranged by size: 

                 938     310     214      54       8
 
BtsI GCAGTG_nn'

Cuts at:      0    1458    1524 
   Size:       1458      66
 
ClaI AT'CG_AT

Cuts at:      0     542    1524 
   Size:        542     982
 
DraIII CAC_nnn'GTG

Cuts at:      0     158    1524 
   Size:        158    1366
 
EaeI y'GGCC_r

Cuts at:      0     489     528     656     671     779     833    1010    1061 
   Size:        489      39     128      15     108      54     177      51

Cuts at:   1061    1097    1121    1405    1424    1524 
   Size:         36      24     284      19     100

  Fragments arranged by size: 

                 489     284     177     128     108     100      54      51
                  39      36      24      19      15
 
EagI C'GGCC_G

Cuts at:      0     656     833    1524 
   Size:        656     177     691

  Fragments arranged by size: 

                 691     656     177
 
EarI CTCTTCn'nnn_

Cuts at:      0      21    1524 
   Size:         21    1503
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256    1051    1245    1524 
   Size:        256     795     194     279

  Fragments arranged by size: 

                 795     279     256     194
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      44     892    1524 
   Size:         44     848     632

  Fragments arranged by size: 

                 848     632      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0      50     144     330    1089    1354    1524 
   Size:         50      94     186     759     265     170

  Fragments arranged by size: 

                 759     265     186     170      94      50
 
HaeII r_GCGC'y

Cuts at:      0     141     316     608    1180    1524 
   Size:        141     175     292     572     344

  Fragments arranged by size: 

                 572     344     292     175     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      42      74     707     739     945     977    1524 
   Size:         42      32     633      32     206      32     547

  Fragments arranged by size: 

                 633     547     206      42      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1042    1524 
   Size:       1042     482
 
KasI G'GCGC_C

Cuts at:      0    1176    1524 
   Size:       1176     348
 
KpnI G_GTAC'C

Cuts at:      0     264    1326    1524 
   Size:        264    1062     198

  Fragments arranged by size: 

                1062     264     198
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1196    1283    1524 
   Size:       1196      87     241

  Fragments arranged by size: 

                1196     241      87
 
MscI TGG'CCA

Cuts at:      0     673    1099    1123    1524 
   Size:        673     426      24     401

  Fragments arranged by size: 

                 673     426     401      24
 
MslI CAynn'nnrTG

Cuts at:      0     281     574     910    1524 
   Size:        281     293     336     614

  Fragments arranged by size: 

                 614     336     293     281
 
MspA1I CmG'CkG

Cuts at:      0     307     408    1379    1524 
   Size:        307     101     971     145

  Fragments arranged by size: 

                 971     307     145     101
 
NaeI GCC'GGC

Cuts at:      0    1156    1405    1524 
   Size:       1156     249     119

  Fragments arranged by size: 

                1156     249     119
 
NarI GG'CG_CC

Cuts at:      0    1177    1524 
   Size:       1177     347
 
NdeI CA'TA_TG

Cuts at:      0    1260    1524 
   Size:       1260     264
 
NgoMIV G'CCGG_C

Cuts at:      0    1154    1403    1524 
   Size:       1154     249     121

  Fragments arranged by size: 

                1154     249     121
 
PfoI T'CCnGG_A

Cuts at:      0     233     821    1524 
   Size:        233     588     703

  Fragments arranged by size: 

                 703     588     233
 
PmlI CAC'GTG

Cuts at:      0     921    1008    1524 
   Size:        921      87     516

  Fragments arranged by size: 

                 921     516      87
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     538     570    1524 
   Size:        538      32     954

  Fragments arranged by size: 

                 954     538      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      50    1089    1524 
   Size:         50    1039     435

  Fragments arranged by size: 

                1039     435      50
 
PshAI GACnn'nnGTC

Cuts at:      0     835    1524 
   Size:        835     689
 
PspOMI G'GGCC_C

Cuts at:      0     143    1524 
   Size:        143    1381
 
PstI C_TGCA'G

Cuts at:      0     413     701     755     995    1524 
   Size:        413     288      54     240     529

  Fragments arranged by size: 

                 529     413     288     240      54
 
PvuII CAG'CTG

Cuts at:      0     307     408    1379    1524 
   Size:        307     101     971     145

  Fragments arranged by size: 

                 971     307     145     101
 
SanDI GG'GwC_CC

Cuts at:      0      50    1524 
   Size:         50    1474
 
SbfI CC_TGCA'GG

Cuts at:      0     755    1524 
   Size:        755     769
 
SexAI A'CCwGG_T

Cuts at:      0     863     935    1091    1524 
   Size:        863      72     156     433

  Fragments arranged by size: 

                 863     433     156      72
 
SfcI C'TryA_G

Cuts at:      0     409     697     751     991    1524 
   Size:        409     288      54     240     533

  Fragments arranged by size: 

                 533     409     288     240      54
 
SfoI GGC'GCC

Cuts at:      0    1178    1524 
   Size:       1178     346
 
SmaI CCC'GGG

Cuts at:      0     149     958    1441    1524 
   Size:        149     809     483      83

  Fragments arranged by size: 

                 809     483     149      83
 
SmlI C'TyrA_G

Cuts at:      0    1149    1524 
   Size:       1149     375
 
StuI AGG'CCT

Cuts at:      0     241     817    1524 
   Size:        241     576     707

  Fragments arranged by size: 

                 707     576     241
 
StyI C'CwwG_G

Cuts at:      0    1100    1524 
   Size:       1100     424
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     169    1524 
   Size:        169    1355
 
TatI w'GTAC_w

Cuts at:      0     902    1524 
   Size:        902     622
 
Tth111I GACn'n_nGTC

Cuts at:      0     223     469     651    1524 
   Size:        223     246     182     873

  Fragments arranged by size: 

                 873     246     223     182
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     137    1524 
   Size:        137    1387
 
XmaI C'CCGG_G

Cuts at:      0     147     956    1439    1524 
   Size:        147     809     483      85

  Fragments arranged by size: 

                 809     483     147      85
 
ZraI GAC'GTC

Cuts at:      0     498    1524 
   Size:        498    1026
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AhdI     AleI     AloI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI   DraIII
     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     PfoI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII    SanDI     SbfI    SexAI     SfcI     SfoI
     SmaI     SmlI     StuI     StyI    TaqII     TatI  Tth111I     XcmI
     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AlfI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BmtI   Bpu10I    BsaBI    BsaWI
    BsiWI     BsmI    BspEI    BsrDI   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI     DrdI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     MfeI     MluI
     NcoI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     ScaI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI
     XhoI     XmnI
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