################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BclI BfrI BglII BlnI BsaWI BseAI BsiEI BspDI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI HindIII HpaI KpnI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI TfiI XbaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 295 GTCTAC GT^[AC][GT]AC AhdI 766 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2265 GTGCAC G^TGCAC AocI 750 CCTGAGG CC^T[ACGT]AGG ApaLI 2265 GTGCAC G^TGCAC AspEI 766 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1196 TGCGCA TGC^GCA BalI 2054 TGGCCA TGG^CCA BamHI 1691 GGATCC G^GATCC BbeI 2886 GGCGCC GGCGC^C BbrPI 2406 CACGTG CAC^GTG BbsI 231 GAAGAC GAAGAC(2/6) BbvCI 573 GCTGAGG CCTCAGC(-5/-2) BcgI 489 CGAGACCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2181 GGATAC GTATCC(6/5) BfaI 1582 CTAG C^TAG BpuAI 231 GAAGAC GAAGAC(2/6) BsaAI 2406 CACGTG [CT]AC^GT[GA] BsaBI 2745 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 90 CGTACG C^GTACG BsmI 1041 GAATGC GAATGC(1/-1) Bsp1407I 1451 TGTACA T^GTACA BsrGI 1451 TGTACA T^GTACA BssSI 2494 CTCGTG CACGAG(-5/-1) BstAPI 690 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2384 CCAGCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 750 CCTGAGG CC^T[ACGT]AGG DraIII 2155 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 766 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 750 CCTGAGG CC^T[ACGT]AGG FspI 1196 TGCGCA TGC^GCA KasI 2886 GGCGCC G^GCGCC MaeI 1582 CTAG C^TAG MamI 2745 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2054 TGGCCA TGG^CCA MscI 2054 TGGCCA TGG^CCA MseI 2552 TTAA T^TAA NarI 2886 GGCGCC GG^CGCC NcoI 2057 CCATGG C^CATGG PaeR7I 236 CTCGAG C^TCGAG PmaCI 2406 CACGTG CAC^GTG PmlI 2406 CACGTG CAC^GTG SacI 1576 GAGCTC GAGCT^C SexAI 1181 ACCAGGT A^CC[AT]GGT SfoI 2886 GGCGCC GGC^GCC SspBI 1451 TGTACA T^GTACA StuI 2594 AGGCCT AGG^CCT Tru9I 2552 TTAA T^TAA Tsp509I 734 AATT ^AATT XhoI 236 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AatII 292 GACGTC GACGT^C 2710 GACGTC AspI 1180 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2722 GACAGTGTC BglI 199 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2008 GCCAAAGTGGC BsmBI 2772 CGTCTC CGTCTC(1/5) 1319 GAGACG EarI 970 GAAGAG CTCTTC(1/4) 1002 GAAGAG EcoT22I 1300 ATGCAT ATGCA^T 1730 ATGCAT HaeII 196 AGCGCC [GA]GCGC^[CT] 2886 GGCGCC HgaI 1661 GCGTC GACGC(5/10) 1751 GCGTC Ksp632I 970 GAAGAG CTCTTC(1/4) 1002 GAAGAG MslI 559 CACTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2433 CACCCTGGTG NsiI 1300 ATGCAT ATGCA^T 1730 ATGCAT NspI 1302 GCATGC [GA]CATG^[CT] 2148 GCATGC PflFI 1180 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2722 GACAGTGTC PpuMI 1997 AGGACCT [GA]G^G[AT]CC[CT] 2102 AGGTCCT SbfI 402 CCTGCAGG CCTGCA^GG 663 CCTGCAGG ScaI 962 AGTACT AGT^ACT 2570 AGTACT SphI 1302 GCATGC GCATG^C 2148 GCATGC Sse8387I 402 CCTGCAGG CCTGCA^GG 663 CCTGCAGG Tth111I 1180 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2722 GACAGTGTC XcmI 254 CCATCGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1388 CCAGCCGCGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI