Restriction map for
pF1KE3344
(Linear) (Six-base) MAPSORT of: KIEE3344.seq Check: 1857 from: 1 to: 3012
KIEE3344.orf
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1152 2076 3012
Size: 1152 924 936
Fragments arranged by size:
1152 936 924
AatII G_ACGT'C
Cuts at: 0 296 2714 3012
Size: 296 2418 298
Fragments arranged by size:
2418 298 296
AccI GT'mk_AC
Cuts at: 0 296 3012
Size: 296 2716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 295 318 601 706 820 3012
Size: 250 45 23 283 105 114 2192
Fragments arranged by size:
2192 283 250 114 105 45 23
AhdI GACnn_n'nnGTC
Cuts at: 0 771 3012
Size: 771 2241
AleI CACnn'nnGTG
Cuts at: 0 2437 3012
Size: 2437 575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 3012
Size: 640 2372
AlwNI CAG_nnn'CTG
Cuts at: 0 465 696 750 870 900 987 1134 1290
Size: 465 231 54 120 30 87 147 156
Cuts at: 1290 1331 1449 1502 2001 2307 2729 2847 3012
Size: 41 118 53 499 306 422 118 165
Fragments arranged by size:
499 465 422 306 231 165 156 147
120 118 118 87 54 53 41 30
ApaLI G'TGCA_C
Cuts at: 0 2265 3012
Size: 2265 747
AvaI C'yCGr_G
Cuts at: 0 236 1527 1797 1830 2022 3012
Size: 236 1291 270 33 192 990
Fragments arranged by size:
1291 990 270 236 192 33
BamHI G'GATC_C
Cuts at: 0 1691 3012
Size: 1691 1321
BanI G'GyrC_C
Cuts at: 0 455 1834 2197 2439 2827 2886 3012
Size: 455 1379 363 242 388 59 126
Fragments arranged by size:
1379 455 388 363 242 126 59
BanII G_rGCy'C
Cuts at: 0 1268 1536 1580 1804 1874 2223 2401 3007
Size: 1268 268 44 224 70 349 178 606
Cuts at: 3007 3012
Size: 5
Fragments arranged by size:
1268 606 349 268 224 178 70 44
5
BbeI G_GCGC'C
Cuts at: 0 2890 3012
Size: 2890 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 238 3012
Size: 238 2774
BbvCI CC'TCA_GC
Cuts at: 0 574 3012
Size: 574 2438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 512 3012
Size: 478 34 2500
Fragments arranged by size:
2500 478 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2175 3012
Size: 2175 837
BfrBI ATG'CAT
Cuts at: 0 1302 1732 3012
Size: 1302 430 1280
Fragments arranged by size:
1302 1280 430
BglI GCCn_nnn'nGGC
Cuts at: 0 205 2014 3012
Size: 205 1809 998
Fragments arranged by size:
1809 998 205
BlpI GC'TnA_GC
Cuts at: 0 595 2222 2944 3012
Size: 595 1627 722 68
Fragments arranged by size:
1627 722 595 68
Bme1580I G_kGCm'C
Cuts at: 0 215 1837 2269 2444 2501 2830 3012
Size: 215 1622 432 175 57 329 182
Fragments arranged by size:
1622 432 329 215 182 175 57
BmgBI CAC'GTC
Cuts at: 0 1385 2112 2772 3012
Size: 1385 727 660 240
Fragments arranged by size:
1385 727 660 240
BmrI ACTGGGnnnn_n'
Cuts at: 0 975 3012
Size: 975 2037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1164 3012
Size: 1164 1848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 538 880 1401 1528 1759 1912 1927
Size: 286 252 342 521 127 231 153 15
Cuts at: 1927 1978 2023 2199 2385 2619 2869 2943 3012
Size: 51 45 176 186 234 250 74 69
Fragments arranged by size:
521 342 286 252 250 234 231 186
176 153 127 74 69 51 45 15
Bpu10I CC'TnA_GC
Cuts at: 0 574 1547 1696 2521 3012
Size: 574 973 149 825 491
Fragments arranged by size:
973 825 574 491 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 2595 2788 3012
Size: 1225 1370 193 224
Fragments arranged by size:
1370 1225 224 193
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 484 1076 1517 2867 3012
Size: 355 129 592 441 1350 145
Fragments arranged by size:
1350 592 441 355 145 129
BsaAI yAC'GTr
Cuts at: 0 2408 3012
Size: 2408 604
BsaBI GATnn'nnATC
Cuts at: 0 2749 3012
Size: 2749 263
BsaHI Gr'CG_yC
Cuts at: 0 293 1661 2711 2887 3012
Size: 293 1368 1050 176 125
Fragments arranged by size:
1368 1050 293 176 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 661 691 2522 2552 3012
Size: 40 30 591 30 1831 30 460
Fragments arranged by size:
1831 591 460 40 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 175 178 424 685 799 820 1924
Size: 61 114 3 246 261 114 21 1104
Cuts at: 1924 2482 2530 2599 2851 2881 3012
Size: 558 48 69 252 30 131
Fragments arranged by size:
1104 558 261 252 246 131 114 114
69 61 48 30 21 3
BseYI C'CCAG_C
Cuts at: 0 106 385 1435 2383 2651 3012
Size: 106 279 1050 948 268 361
Fragments arranged by size:
1050 948 361 279 268 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 493 592 2092 2715 2722 3012
Size: 157 336 99 1500 623 7 290
Fragments arranged by size:
1500 623 336 290 157 99 7
BsiHKAI G_wGCw'C
Cuts at: 0 476 1113 1241 1580 2269 2492 3012
Size: 476 637 128 339 689 223 520
Fragments arranged by size:
689 637 520 476 339 223 128
BsiWI C'GTAC_G
Cuts at: 0 90 3012
Size: 90 2922
BsmI GAATG_Cn'
Cuts at: 0 1047 3012
Size: 1047 1965
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1313 2778 3012
Size: 1313 1465 234
Fragments arranged by size:
1465 1313 234
Bsp1286I G_dGCh'C
Cuts at: 0 215 476 1113 1241 1268 1536 1580 1804
Size: 215 261 637 128 27 268 44 224
Cuts at: 1804 1837 1874 2223 2269 2401 2444 2492 2501
Size: 33 37 349 46 132 43 48 9
Cuts at: 2501 2830 3007 3012
Size: 329 177 5
Fragments arranged by size:
637 349 329 268 261 224 215 177
132 128 48 46 44 43 37 33
27 9 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 1152 2076 3012
Size: 134 1018 924 936
Fragments arranged by size:
1018 936 924 134
BsrBI CCG'CTC
Cuts at: 0 930 1415 1658 3012
Size: 930 485 243 1354
Fragments arranged by size:
1354 930 485 243
BsrDI GCAATG_nn'
Cuts at: 0 624 1732 2091 3012
Size: 624 1108 359 921
Fragments arranged by size:
1108 921 624 359
BsrGI T'GTAC_A
Cuts at: 0 1451 3012
Size: 1451 1561
BssSI C'ACGA_G
Cuts at: 0 2494 3012
Size: 2494 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 696 3012
Size: 696 2316
BstEII G'GTnAC_C
Cuts at: 0 306 1704 1791 2655 3012
Size: 306 1398 87 864 357
Fragments arranged by size:
1398 864 357 306 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2391 3012
Size: 2391 621
BstYI r'GATC_y
Cuts at: 0 1511 1691 2516 3012
Size: 1511 180 825 496
Fragments arranged by size:
1511 825 496 180
Bsu36I CC'TnA_GG
Cuts at: 0 751 3012
Size: 751 2261
BtgI C'CryG_G
Cuts at: 0 935 1273 1916 2057 3012
Size: 935 338 643 141 955
Fragments arranged by size:
955 935 643 338 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 240 2949 3012
Size: 240 2709 63
Fragments arranged by size:
2709 240 63
BtsI GCAGTG_nn'
Cuts at: 0 2269 3012
Size: 2269 743
DraIII CAC_nnn'GTG
Cuts at: 0 2160 3012
Size: 2160 852
EaeI y'GGCC_r
Cuts at: 0 485 641 1463 2054 3012
Size: 485 156 822 591 958
Fragments arranged by size:
958 822 591 485 156
EarI CTCTTCn'nnn_
Cuts at: 0 965 997 3012
Size: 965 32 2015
Fragments arranged by size:
2015 965 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 859 1689 1825 2944 3012
Size: 859 830 136 1119 68
Fragments arranged by size:
1119 859 830 136 68
EcoICRI GAG'CTC
Cuts at: 0 1578 3012
Size: 1578 1434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 286 295 318 538 601 706 820
Size: 250 36 9 23 220 63 105 114
Cuts at: 820 880 1401 1528 1759 1912 1927 1978 2023
Size: 60 521 127 231 153 15 51 45
Cuts at: 2023 2199 2385 2619 2869 2943 3012
Size: 176 186 234 250 74 69
Fragments arranged by size:
521 250 250 234 231 220 186 176
153 127 114 105 74 69 63 60
51 45 36 23 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 406 667 802 874 1699 1909 2245
Size: 130 276 261 135 72 825 210 336
Cuts at: 2245 2364 3012
Size: 119 648
Fragments arranged by size:
825 648 336 276 261 210 135 130
119 72
EcoO109I rG'GnC_Cy
Cuts at: 0 504 1998 2103 2811 3012
Size: 504 1494 105 708 201
Fragments arranged by size:
1494 708 504 201 105
FspI TGC'GCA
Cuts at: 0 1198 3012
Size: 1198 1814
HaeII r_GCGC'y
Cuts at: 0 200 2890 3012
Size: 200 2690 122
Fragments arranged by size:
2690 200 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 2600 2632 3012
Size: 757 32 1811 32 380
Fragments arranged by size:
1811 757 380 32 32
HincII GTy'rAC
Cuts at: 0 330 1968 2049 2781 3012
Size: 330 1638 81 732 231
Fragments arranged by size:
1638 732 330 231 81
KasI G'GCGC_C
Cuts at: 0 2886 3012
Size: 2886 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 2357 3012
Size: 1349 1008 655
Fragments arranged by size:
1349 1008 655
MscI TGG'CCA
Cuts at: 0 2056 3012
Size: 2056 956
MslI CAynn'nnrTG
Cuts at: 0 563 2437 3012
Size: 563 1874 575
Fragments arranged by size:
1874 575 563
MspA1I CmG'CkG
Cuts at: 0 153 465 516 1215 1287 1502 1605 2648
Size: 153 312 51 699 72 215 103 1043
Cuts at: 2648 2718 2820 2948 3012
Size: 70 102 128 64
Fragments arranged by size:
1043 699 312 215 153 128 103 102
72 70 64 51
NarI GG'CG_CC
Cuts at: 0 2887 3012
Size: 2887 125
NcoI C'CATG_G
Cuts at: 0 2057 3012
Size: 2057 955
NsiI A_TGCA'T
Cuts at: 0 1304 1734 3012
Size: 1304 430 1278
Fragments arranged by size:
1304 1278 430
NspI r_CATG'y
Cuts at: 0 1306 2152 3012
Size: 1306 846 860
Fragments arranged by size:
1306 860 846
PasI CC'CwG_GG
Cuts at: 0 2501 3012
Size: 2501 511
PflMI CCAn_nnn'nTGG
Cuts at: 0 1134 2001 2290 2670 3012
Size: 1134 867 289 380 342
Fragments arranged by size:
1134 867 380 342 289
PfoI T'CCnGG_A
Cuts at: 0 263 1514 1889 1994 2246 2621 2705 2846
Size: 263 1251 375 105 252 375 84 141
Cuts at: 2846 3012
Size: 166
Fragments arranged by size:
1251 375 375 263 252 166 141 105
84
PmlI CAC'GTG
Cuts at: 0 2408 3012
Size: 2408 604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1861 1893 3012
Size: 1861 32 1119
Fragments arranged by size:
1861 1119 32
PpuMI rG'GwC_Cy
Cuts at: 0 1998 2103 3012
Size: 1998 105 909
Fragments arranged by size:
1998 909 105
PsiI TTA'TAA
Cuts at: 0 1982 3012
Size: 1982 1030
PspXI vC'TCGA_Gb
Cuts at: 0 236 3012
Size: 236 2776
PstI C_TGCA'G
Cuts at: 0 320 407 470 629 668 701 995 1052
Size: 320 87 63 159 39 33 294 57
Cuts at: 1052 1073 1220 1336 1622 2087 3012
Size: 21 147 116 286 465 925
Fragments arranged by size:
925 465 320 294 286 159 147 116
87 63 57 39 33 21
PvuII CAG'CTG
Cuts at: 0 153 465 516 1215 1502 1605 2718 2948
Size: 153 312 51 699 287 103 1113 230
Cuts at: 2948 3012
Size: 64
Fragments arranged by size:
1113 699 312 287 230 153 103 64
51
SacI G_AGCT'C
Cuts at: 0 1580 3012
Size: 1580 1432
SbfI CC_TGCA'GG
Cuts at: 0 407 668 3012
Size: 407 261 2344
Fragments arranged by size:
2344 407 261
ScaI AGT'ACT
Cuts at: 0 964 2572 3012
Size: 964 1608 440
Fragments arranged by size:
1608 964 440
SexAI A'CCwGG_T
Cuts at: 0 1181 3012
Size: 1181 1831
SfcI C'TryA_G
Cuts at: 0 316 403 466 612 625 664 697 991
Size: 316 87 63 146 13 39 33 294
Cuts at: 991 1026 1048 1069 1216 1332 1618 2083 2815
Size: 35 22 21 147 116 286 465 732
Cuts at: 2815 3012
Size: 197
Fragments arranged by size:
732 465 316 294 286 197 147 146
116 87 63 39 35 33 22 21
13
SfoI GGC'GCC
Cuts at: 0 2888 3012
Size: 2888 124
SmaI CCC'GGG
Cuts at: 0 1529 1799 1832 3012
Size: 1529 270 33 1180
Fragments arranged by size:
1529 1180 270 33
SmlI C'TyrA_G
Cuts at: 0 236 1240 2574 2803 3012
Size: 236 1004 1334 229 209
Fragments arranged by size:
1334 1004 236 229 209
SphI G_CATG'C
Cuts at: 0 1306 2152 3012
Size: 1306 846 860
Fragments arranged by size:
1306 860 846
SrfI GCCC'GGGC
Cuts at: 0 1832 3012
Size: 1832 1180
StuI AGG'CCT
Cuts at: 0 2596 3012
Size: 2596 416
StyI C'CwwG_G
Cuts at: 0 137 2057 2336 2544 3012
Size: 137 1920 279 208 468
Fragments arranged by size:
1920 468 279 208 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 388 1852 2643 2710 3012
Size: 388 1464 791 67 302
Fragments arranged by size:
1464 791 388 302 67
TatI w'GTAC_w
Cuts at: 0 962 1451 2570 3012
Size: 962 489 1119 442
Fragments arranged by size:
1119 962 489 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 3012
Size: 40 32 2940
Fragments arranged by size:
2940 40 32
Tth111I GACn'n_nGTC
Cuts at: 0 1183 2725 3012
Size: 1183 1542 287
Fragments arranged by size:
1542 1183 287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 1395 3012
Size: 261 1134 1617
Fragments arranged by size:
1617 1134 261
XhoI C'TCGA_G
Cuts at: 0 236 3012
Size: 236 2776
XmaI C'CCGG_G
Cuts at: 0 1527 1797 1830 3012
Size: 1527 270 33 1182
Fragments arranged by size:
1527 1182 270 33
ZraI GAC'GTC
Cuts at: 0 294 2712 3012
Size: 294 2418 300
Fragments arranged by size:
2418 300 294
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI AlwNI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PsiI PspXI PstI PvuII SacI SbfI ScaI
SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BclI BglII
BmtI BsaWI BsiEI BspEI BspHI BsrFI BssHII BstBI
BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PciI
PmeI PshAI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SapI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI XbaI XmnI