(Circular) (Six-base) MAPSORT of: pF1KE0282.seq Check: 6061 from: 1 to: 4409 pF1KE0282 4409 bp With 182 enzymes: SgfI * October 18, 2013 09:44 .. AccI GT'mk_AC Cuts at: 637 999 1447 637 Size: 362 448 3599 Fragments arranged by size: 3599 448 362 Acc65I G'GTAC_C Cuts at: 1425 1425 Size: 4409 AclI AA'CG_TT Cuts at: 4249 4249 Size: 4409 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 572 1403 2234 2666 3413 3681 572 Size: 831 831 432 747 268 1300 Fragments arranged by size: 1300 831 831 747 432 268 AflIII A'CryG_T Cuts at: 259 1761 2865 3842 259 Size: 1502 1104 977 826 Fragments arranged by size: 1502 1104 977 826 AgeI A'CCGG_T Cuts at: 2797 2797 Size: 4409 AhdI GACnn_n'nnGTC Cuts at: 1395 1395 Size: 4409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 202 202 Size: 4409 AlwNI CAG_nnn'CTG Cuts at: 1714 3281 1714 Size: 1567 2842 ApaLI G'TGCA_C Cuts at: 3179 3179 Size: 4409 ApoI r'AATT_y Cuts at: 287 491 1413 3762 4018 287 Size: 204 922 2349 256 678 Fragments arranged by size: 2349 922 678 256 204 AseI AT'TA_AT Cuts at: 20 20 Size: 4409 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4409 AvaI C'yCGr_G Cuts at: 725 1040 1429 725 Size: 315 389 3705 Fragments arranged by size: 3705 389 315 BamHI G'GATC_C Cuts at: 1434 1434 Size: 4409 BanI G'GyrC_C Cuts at: 1425 1839 2087 2122 1425 Size: 414 248 35 3712 Fragments arranged by size: 3712 414 248 35 BanII G_rGCy'C Cuts at: 328 960 1423 2453 328 Size: 632 463 1030 2284 Fragments arranged by size: 2284 1030 632 463 BbeI G_GCGC'C Cuts at: 1843 2091 1843 Size: 248 4161 BbsI GAAGACnn'nnnn_ Cuts at: 443 1316 1376 443 Size: 873 60 3476 Fragments arranged by size: 3476 873 60 BciVI GTATCCnnnnn_n' Cuts at: 2307 3068 4409 2307 Size: 761 1341 2307 Fragments arranged by size: 2307 1341 761 BclI T'GATC_A Cuts at: 1929 1929 Size: 4409 BglI GCCn_nnn'nGGC Cuts at: 473 473 Size: 4409 BglII A'GATC_T Cuts at: 1138 1924 3961 1138 Size: 786 2037 1586 Fragments arranged by size: 2037 1586 786 BlpI GC'TnA_GC Cuts at: 1521 1521 Size: 4409 Bme1580I G_kGCm'C Cuts at: 2034 2127 3183 2034 Size: 93 1056 3260 Fragments arranged by size: 3260 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 714 1621 1702 2037 4181 714 Size: 907 81 335 2144 942 Fragments arranged by size: 2144 942 907 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 342 2776 342 Size: 2434 1975 Bpu10I CC'TnA_GC Cuts at: 622 622 Size: 4409 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 990 1585 2464 2956 3254 990 Size: 595 879 492 298 2145 Fragments arranged by size: 2145 879 595 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1043 4113 1043 Size: 3070 1339 BsaAI yAC'GTr Cuts at: 1762 1762 Size: 4409 BsaBI GATnn'nnATC Cuts at: 6 299 1923 3960 6 Size: 293 1624 2037 455 Fragments arranged by size: 2037 1624 455 293 BsaHI Gr'CG_yC Cuts at: 1840 2088 2790 4286 1840 Size: 248 702 1496 1963 Fragments arranged by size: 1963 1496 702 248 BsaWI w'CCGG_w Cuts at: 1285 1598 2119 2797 3071 3218 3629 1285 Size: 313 521 678 274 147 411 2065 Fragments arranged by size: 2065 678 521 411 313 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1071 1101 1191 1221 1071 Size: 30 90 30 4259 Fragments arranged by size: 4259 90 30 30 BseYI C'CCAG_C Cuts at: 1834 2592 3169 3665 1834 Size: 758 577 496 2578 Fragments arranged by size: 2578 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 641 641 Size: 4409 BsiEI CG_ry'CG Cuts at: 104 119 1951 1997 3205 104 Size: 15 1832 46 1208 1308 Fragments arranged by size: 1832 1308 1208 46 15 BsiHKAI G_wGCw'C Cuts at: 328 1423 2201 2391 3183 328 Size: 1095 778 190 792 1554 Fragments arranged by size: 1554 1095 792 778 190 Bsp1286I G_dGCh'C Cuts at: 328 960 1423 2034 2127 2201 2391 2453 3183 Size: 632 463 611 93 74 190 62 730 Cuts at: 3183 328 Size: 1554 Fragments arranged by size: 1554 730 632 611 463 190 93 74 62 BspEI T'CCGG_A Cuts at: 3629 3629 Size: 4409 BspHI T'CATG_A Cuts at: 291 335 3585 291 Size: 44 3250 1115 Fragments arranged by size: 3250 1115 44 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1025 1459 1673 1975 2356 1025 Size: 434 214 302 381 3078 Fragments arranged by size: 3078 434 381 302 214 BsrBI CCG'CTC Cuts at: 50 2701 2755 4239 4404 50 Size: 2651 54 1484 165 55 Fragments arranged by size: 2651 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2321 2321 Size: 4409 BsrFI r'CCGG_y Cuts at: 2407 2588 2797 2407 Size: 181 209 4019 Fragments arranged by size: 4019 209 181 BssSI C'ACGA_G Cuts at: 199 959 2680 3038 199 Size: 760 1721 358 1570 Fragments arranged by size: 1721 1570 760 358 BstAPI GCAn_nnn'nTGC Cuts at: 1786 1786 Size: 4409 BstBI TT'CG_AA Cuts at: 2770 2770 Size: 4409 BstYI r'GATC_y Cuts at: 1 1138 1255 1434 1901 1924 2259 2505 3517 Size: 1137 117 179 467 23 335 246 1012 Cuts at: 3517 3603 3615 3961 1 Size: 86 12 346 449 Fragments arranged by size: 1137 1012 467 449 346 335 246 179 117 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2359 2540 2703 2359 Size: 181 163 4065 Fragments arranged by size: 4065 181 163 BtsI GCAGTG_nn' Cuts at: 1652 1652 Size: 4409 ClaI AT'CG_AT Cuts at: 1119 1119 Size: 4409 DraI TTT'AAA Cuts at: 1408 1408 Size: 4409 DrdI GACnn_nn'nnGTC Cuts at: 2115 2973 2115 Size: 858 3551 EaeI y'GGCC_r Cuts at: 116 1994 2168 2559 2586 3931 116 Size: 1878 174 391 27 1345 594 Fragments arranged by size: 1878 1345 594 391 174 27 EagI C'GGCC_G Cuts at: 116 1994 116 Size: 1878 2531 EarI CTCTTCn'nnn_ Cuts at: 316 602 926 2432 2642 316 Size: 286 324 1506 210 2083 Fragments arranged by size: 2083 1506 324 286 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1092 2617 2927 3073 4219 1092 Size: 1525 310 146 1146 1282 Fragments arranged by size: 1525 1282 1146 310 146 EcoICRI GAG'CTC Cuts at: 326 1421 326 Size: 1095 3314 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 572 1403 2234 2666 3413 3681 572 Size: 831 831 432 747 268 1300 Fragments arranged by size: 1300 831 831 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1548 1548 Size: 4409 EcoRI G'AATT_C Cuts at: 1413 1413 Size: 4409 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2428 2428 Size: 4409 FspI TGC'GCA Cuts at: 2190 2190 Size: 4409 HaeII r_GCGC'y Cuts at: 1843 2091 3113 4092 1843 Size: 248 1022 979 2160 Fragments arranged by size: 2160 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 706 738 1168 1191 1200 1216 1223 1248 1363 Size: 32 430 23 9 16 7 25 115 Cuts at: 1363 1381 1395 1413 2251 2281 2283 2313 706 Size: 18 14 18 838 30 2 30 2802 Fragments arranged by size: 2802 838 430 115 32 30 30 25 23 18 18 16 14 9 7 2 HincII GTy'rAC Cuts at: 638 1448 638 Size: 810 3599 HindIII A'AGCT_T Cuts at: 648 648 Size: 4409 KasI G'GCGC_C Cuts at: 1839 2087 1839 Size: 248 4161 KpnI G_GTAC'C Cuts at: 1429 1429 Size: 4409 MfeI C'AATT_G Cuts at: 641 641 Size: 4409 MluI A'CGCG_T Cuts at: 3842 3842 Size: 4409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 110 3080 3264 110 Size: 2970 184 1255 Fragments arranged by size: 2970 1255 184 MscI TGG'CCA Cuts at: 2170 2170 Size: 4409 MslI CAynn'nnrTG Cuts at: 2398 2525 2398 Size: 127 4282 MspA1I CmG'CkG Cuts at: 463 1520 1834 2194 3207 3452 463 Size: 1057 314 360 1013 245 1420 Fragments arranged by size: 1420 1057 1013 360 314 245 NaeI GCC'GGC Cuts at: 2590 2590 Size: 4409 NarI GG'CG_CC Cuts at: 1840 2088 1840 Size: 248 4161 NgoMIV G'CCGG_C Cuts at: 2588 2588 Size: 4409 NspI r_CATG'y Cuts at: 1462 1962 2869 1462 Size: 500 907 3002 Fragments arranged by size: 3002 907 500 PasI CC'CwG_GG Cuts at: 573 573 Size: 4409 PciI A'CATG_T Cuts at: 2865 2865 Size: 4409 PflMI CCAn_nnn'nTGG Cuts at: 744 744 Size: 4409 PmeI GTTT'AAAC Cuts at: 1408 1408 Size: 4409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 972 1004 3548 3580 972 Size: 32 2544 32 1801 Fragments arranged by size: 2544 1801 32 32 PstI C_TGCA'G Cuts at: 1456 2141 1456 Size: 685 3724 PvuI CG_AT'CG Cuts at: 104 104 Size: 4409 PvuII CAG'CTG Cuts at: 1834 2194 1834 Size: 360 4049 RsrII CG'GwC_CG Cuts at: 2604 2604 Size: 4409 SacI G_AGCT'C Cuts at: 328 1423 328 Size: 1095 3314 SalI G'TCGA_C Cuts at: 636 1446 636 Size: 810 3599 SapI GCTCTTCn'nnn_ Cuts at: 316 2432 2642 316 Size: 2116 210 2083 Fragments arranged by size: 2116 2083 210 SbfI CC_TGCA'GG Cuts at: 1456 1456 Size: 4409 ScaI AGT'ACT Cuts at: 3926 3926 Size: 4409 SfcI C'TryA_G Cuts at: 33 785 1452 2137 3130 3321 33 Size: 752 667 685 993 191 1121 Fragments arranged by size: 1121 993 752 685 667 191 SfoI GGC'GCC Cuts at: 1841 2089 1841 Size: 248 4161 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4409 SmaI CCC'GGG Cuts at: 727 1431 727 Size: 704 3705 SmlI C'TyrA_G Cuts at: 1005 1564 2479 2971 3233 1005 Size: 559 915 492 262 2181 Fragments arranged by size: 2181 915 559 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4409 SphI G_CATG'C Cuts at: 1462 1962 1462 Size: 500 3909 SspI AAT'ATT Cuts at: 217 217 Size: 4409 StyI C'CwwG_G Cuts at: 1235 1543 1897 1235 Size: 308 354 3747 Fragments arranged by size: 3747 354 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 167 1992 2125 2584 2793 167 Size: 1825 133 459 209 1783 Fragments arranged by size: 1825 1783 459 209 133 TatI w'GTAC_w Cuts at: 519 3924 519 Size: 3405 1004 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3926 3958 3926 Size: 32 4377 Tth111I GACn'n_nGTC Cuts at: 2206 2206 Size: 4409 XbaI T'CTAG_A Cuts at: 1440 1440 Size: 4409 XcmI CCAnnnn_n'nnnnTGG Cuts at: 451 1904 451 Size: 1453 2956 XmaI C'CCGG_G Cuts at: 725 1429 725 Size: 704 3705 XmnI GAAnn'nnTTC Cuts at: 287 491 987 287 Size: 204 496 3709 Fragments arranged by size: 3709 496 204 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI