################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BbeI BbrPI BbsI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsgI BsiWI BspDI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII ClaI DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 1200 GTATAC GT^[AC][GT]AC AccIII 14 TCCGGA T^CCGGA AflIII 1862 ACATGT A^C[GA][CT]GT Alw44I 664 GTGCAC G^TGCAC ApaI 2228 GGGCCC GGGCC^C ApaLI 664 GTGCAC G^TGCAC AspHI 664 GTGCAC G[AT]GC[AT]^C AvaI 272 CCCGGG C^[CT]CG[GA]G AviII 2247 TGCGCA TGC^GCA BalI 2363 TGGCCA TGG^CCA BanI 992 GGTGCC G^G[CT][GA]CC BanII 2228 GGGCCC G[GA]GC[CT]^C BbvCI 2232 CCTCAGC CCTCAGC(-5/-2) BclI 2375 TGATCA T^GATCA BsaAI 661 TACGTG [CT]AC^GT[GA] BsaBI 2280 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 14 TCCGGA T^CCGGA BsiEI 1465 CGGCCG CG[GA][CT]^CG BsiHKAI 664 GTGCAC G[AT]GC[AT]^C BsmBI 1495 GAGACG CGTCTC(1/5) BsmI 1830 GAATGC GAATGC(1/-1) BsoBI 272 CCCGGG C^[CT]CG[GA]G Bsp1407I 1394 TGTACA T^GTACA BspEI 14 TCCGGA T^CCGGA BspHI 1754 TCATGA T^CATGA BspLU11I 1862 ACATGT A^CATGT BsrDI 1590 CATTGC GCAATG(2/0) BsrGI 1394 TGTACA T^GTACA Bst1107I 1200 GTATAC GTA^TAC BstZ17I 1200 GTATAC GTA^TAC CpoI 2061 CGGACCG CG^G[AT]CCG DrdI 2146 GACACCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1465 CGGCCG C^GGCCG EclXI 1465 CGGCCG C^GGCCG Eco52I 1465 CGGCCG C^GGCCG EcoNI 609 CCTGCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1922 GAATTC G^AATTC FbaI 2375 TGATCA T^GATCA FspI 2247 TGCGCA TGC^GCA HindIII 190 AAGCTT A^AGCTT MamI 2280 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2363 TGGCCA TGG^CCA MroI 14 TCCGGA T^CCGGA MscI 2363 TGGCCA TGG^CCA NdeI 1660 CATATG CA^TATG PflMI 1882 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1246 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 868 CTGCAG CTGCA^G RcaI 1754 TCATGA T^CATGA RsrII 2061 CGGACCG CG^G[AT]CCG SapI 2301 GAAGAGC GCTCTTC(1/4) ScaI 2180 AGTACT AGT^ACT SgrAI 243 CACCGGTG C[GA]^CCGG[CT]G SmaI 272 CCCGGG CCC^GGG SphI 575 GCATGC GCATG^C SspBI 1394 TGTACA T^GTACA TfiI 837 GAATC G^A[AT]TC Van91I 1882 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 272 CCCGGG C^CCGGG XmaI 272 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AgeI 244 ACCGGT A^CCGGT 2455 ACCGGT AlwNI 56 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1883 CAGAGTCTG Asp700I 132 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2436 GAATAAATTC BseRI 2010 GAGGAG GAGGAG(10/8) 2071 GAGGAG BsrFI 244 ACCGGT [GA]^CCGG[CT] 2455 ACCGGT Cac8I 575 GCATGC GC[ACGT]^[ACGT]GC 870 GCAGGC Cfr10I 244 ACCGGT [GA]^CCGG[CT] 2455 ACCGGT DraII 2228 GGGCCCT [GA]G^G[ACGT]CC[CT] 2384 AGGGCCT EaeI 1465 CGGCCG [CT]^GGCC[GA] 2363 TGGCCA EcoO109I 2228 GGGCCCT [GA]G^G[ACGT]CC[CT] 2384 AGGGCCT HgaI 1497 GACGC GACGC(5/10) 2122 GACGC NcoI 2150 CCATGG C^CATGG 2273 CCATGG NspI 575 GCATGC [GA]CATG^[CT] 1862 ACATGT PinAI 244 ACCGGT A^CCGGT 2455 ACCGGT XcmI 1659 CCATATGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2351 CCACCCTGACCGTGG XmnI 132 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2436 GAATAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BamHI BbvI BcnI BmyI BpmI BsaJI BsaWI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII