(Circular) (Six-base) MAPSORT of: pF1KE2585.seq Check: 4046 from: 1 to: 5627 pF1KE2585 5627 bp With 182 enzymes: SgfI * September 4, 2015 15:51 .. AccI GT'mk_AC Cuts at: 1309 2665 1309 Size: 1356 4271 Acc65I G'GTAC_C Cuts at: 2643 2643 Size: 5627 AclI AA'CG_TT Cuts at: 5467 5467 Size: 5627 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 451 481 1619 3452 3884 4631 4899 451 Size: 30 1138 1833 432 747 268 1179 Fragments arranged by size: 1833 1179 1138 747 432 268 30 AflIII A'CryG_T Cuts at: 1970 2979 4083 5060 1970 Size: 1009 1104 977 2537 Fragments arranged by size: 2537 1104 1009 977 AgeI A'CCGG_T Cuts at: 352 2563 4015 352 Size: 2211 1452 1964 Fragments arranged by size: 2211 1964 1452 AleI CACnn'nnGTG Cuts at: 2061 2061 Size: 5627 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1001 1001 Size: 5627 AlwNI CAG_nnn'CTG Cuts at: 169 1996 2932 4499 169 Size: 1827 936 1567 1297 Fragments arranged by size: 1827 1567 1297 936 ApaI G_GGCC'C Cuts at: 2340 2340 Size: 5627 ApaLI G'TGCA_C Cuts at: 772 4397 772 Size: 3625 2002 ApoI r'AATT_y Cuts at: 256 493 2030 2548 2631 4980 5236 256 Size: 237 1537 518 83 2349 256 647 Fragments arranged by size: 2349 1537 647 518 256 237 83 AseI AT'TA_AT Cuts at: 20 20 Size: 5627 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5627 AvaI C'yCGr_G Cuts at: 380 2647 380 Size: 2267 3360 BamHI G'GATC_C Cuts at: 558 750 1415 2652 558 Size: 192 665 1237 3533 Fragments arranged by size: 3533 1237 665 192 BanI G'GyrC_C Cuts at: 1100 2643 3057 3305 3340 1100 Size: 1543 414 248 35 3387 Fragments arranged by size: 3387 1543 414 248 35 BanII G_rGCy'C Cuts at: 2340 2641 3671 2340 Size: 301 1030 4296 Fragments arranged by size: 4296 1030 301 BbeI G_GCGC'C Cuts at: 3061 3309 3061 Size: 248 5379 BbvCI CC'TCA_GC Cuts at: 2341 2341 Size: 5627 BciVI GTATCCnnnnn_n' Cuts at: 3525 4286 5627 3525 Size: 761 1341 3525 Fragments arranged by size: 3525 1341 761 BclI T'GATC_A Cuts at: 2483 3147 2483 Size: 664 4963 BglII A'GATC_T Cuts at: 3142 5179 3142 Size: 2037 3590 BlpI GC'TnA_GC Cuts at: 2739 2739 Size: 5627 Bme1580I G_kGCm'C Cuts at: 776 1105 1150 2340 3252 3345 4401 776 Size: 329 45 1190 912 93 1056 2002 Fragments arranged by size: 2002 1190 1056 912 329 93 45 BmrI ACTGGGnnnn_n' Cuts at: 2538 2839 2920 3255 5399 2538 Size: 301 81 335 2144 2766 Fragments arranged by size: 2766 2144 335 301 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 874 2514 3994 874 Size: 1640 1480 2507 Fragments arranged by size: 2507 1640 1480 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 766 926 1584 1670 766 Size: 160 658 86 4723 Fragments arranged by size: 4723 658 160 86 Bpu10I CC'TnA_GC Cuts at: 2341 2401 2341 Size: 60 5567 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2803 3682 4174 4472 2803 Size: 879 492 298 3958 Fragments arranged by size: 3958 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 5331 5331 Size: 5627 BsaAI yAC'GTr Cuts at: 771 2980 771 Size: 2209 3418 BsaBI GATnn'nnATC Cuts at: 6 2392 3141 5178 6 Size: 2386 749 2037 455 Fragments arranged by size: 2386 2037 749 455 BsaHI Gr'CG_yC Cuts at: 3058 3306 4008 5504 3058 Size: 248 702 1496 3181 Fragments arranged by size: 3181 1496 702 248 BsaWI w'CCGG_w Cuts at: 122 352 2563 2816 3337 4015 4289 4436 4847 Size: 230 2211 253 521 678 274 147 411 Cuts at: 4847 122 Size: 902 Fragments arranged by size: 2211 902 678 521 411 274 253 230 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1105 1135 2197 2227 2302 2332 2414 2444 1105 Size: 30 1062 30 75 30 82 30 4288 Fragments arranged by size: 4288 1062 82 75 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2133 2194 2133 Size: 61 5566 BseYI C'CCAG_C Cuts at: 146 1638 3052 3810 4387 4883 146 Size: 1492 1414 758 577 496 890 Fragments arranged by size: 1492 1414 890 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1576 3169 3215 4423 104 Size: 1472 1593 46 1208 1308 Fragments arranged by size: 1593 1472 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 776 2641 3419 3609 4401 776 Size: 1865 778 190 792 2002 Fragments arranged by size: 2002 1865 792 778 190 BsmI GAATG_Cn' Cuts at: 1944 1944 Size: 5627 BsmBI CGTCTCn'nnnn_ Cuts at: 1597 1597 Size: 5627 Bsp1286I G_dGCh'C Cuts at: 776 1105 1150 2340 2641 3252 3345 3419 3609 Size: 329 45 1190 301 611 93 74 190 Cuts at: 3609 3671 4401 776 Size: 62 730 2002 Fragments arranged by size: 2002 1190 730 611 329 301 190 93 74 62 45 BspEI T'CCGG_A Cuts at: 122 4847 122 Size: 4725 902 BspHI T'CATG_A Cuts at: 1862 4803 1862 Size: 2941 2686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2677 2891 3193 3574 2677 Size: 214 302 381 4730 Fragments arranged by size: 4730 381 302 214 BsrBI CCG'CTC Cuts at: 50 3919 3973 5457 5622 50 Size: 3869 54 1484 165 55 Fragments arranged by size: 3869 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1697 3539 1697 Size: 1842 3785 BsrFI r'CCGG_y Cuts at: 352 2563 3625 3806 4015 352 Size: 2211 1062 181 209 1964 Fragments arranged by size: 2211 1964 1062 209 181 BsrGI T'GTAC_A Cuts at: 1502 1502 Size: 5627 BssSI C'ACGA_G Cuts at: 3898 4256 3898 Size: 358 5269 BstAPI GCAn_nnn'nTGC Cuts at: 3004 3004 Size: 5627 BstBI TT'CG_AA Cuts at: 3988 3988 Size: 5627 BstYI r'GATC_y Cuts at: 1 558 750 1415 2652 3119 3142 3477 3723 Size: 557 192 665 1237 467 23 335 246 Cuts at: 3723 4735 4821 4833 5179 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1237 1012 665 557 467 449 346 335 246 192 86 23 12 BstZ17I GTA'TAC Cuts at: 1310 1310 Size: 5627 BtgI C'CryG_G Cuts at: 107 2258 2381 2468 107 Size: 2151 123 87 3266 Fragments arranged by size: 3266 2151 123 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3577 3758 3921 3577 Size: 181 163 5283 Fragments arranged by size: 5283 181 163 BtsI GCAGTG_nn' Cuts at: 2312 2447 2515 2870 2312 Size: 135 68 355 5069 Fragments arranged by size: 5069 355 135 68 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2130 2165 2130 Size: 35 5592 DraI TTT'AAA Cuts at: 2626 2626 Size: 5627 DrdI GACnn_nn'nnGTC Cuts at: 2260 3333 4191 2260 Size: 1073 858 3696 Fragments arranged by size: 3696 1073 858 EaeI y'GGCC_r Cuts at: 1573 2471 3212 3386 3777 3804 5149 1573 Size: 898 741 174 391 27 1345 2051 Fragments arranged by size: 2051 1345 898 741 391 174 27 EagI C'GGCC_G Cuts at: 1573 3212 1573 Size: 1639 3988 EarI CTCTTCn'nnn_ Cuts at: 457 569 606 1595 2404 2612 3650 3860 457 Size: 112 37 989 809 208 1038 210 2224 Fragments arranged by size: 2224 1038 989 809 210 208 112 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 571 3835 4145 4291 5437 571 Size: 3264 310 146 1146 761 Fragments arranged by size: 3264 1146 761 310 146 EcoICRI GAG'CTC Cuts at: 2639 2639 Size: 5627 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 451 481 766 926 1584 1619 1670 3452 3884 Size: 30 285 160 658 35 51 1782 432 Cuts at: 3884 4631 4899 451 Size: 747 268 1179 Fragments arranged by size: 1782 1179 747 658 432 285 268 160 51 35 30 EcoNI CCTnn'n_nnAGG Cuts at: 721 721 Size: 5627 EcoO109I rG'GnC_Cy Cuts at: 2337 2493 2766 2337 Size: 156 273 5198 Fragments arranged by size: 5198 273 156 EcoRI G'AATT_C Cuts at: 2030 2631 2030 Size: 601 5026 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 486 1624 3646 486 Size: 1138 2022 2467 Fragments arranged by size: 2467 2022 1138 FspI TGC'GCA Cuts at: 2357 3408 2357 Size: 1051 4576 HaeII r_GCGC'y Cuts at: 3061 3309 4331 5310 3061 Size: 248 1022 979 3378 Fragments arranged by size: 3378 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 543 568 575 600 912 944 1105 1137 2197 Size: 25 7 25 312 32 161 32 1060 Cuts at: 2197 2229 3469 3499 3501 3531 543 Size: 32 1240 30 2 30 2639 Fragments arranged by size: 2639 1240 1060 312 161 32 32 32 30 30 25 25 7 2 HincII GTy'rAC Cuts at: 2666 2666 Size: 5627 HindIII A'AGCT_T Cuts at: 298 298 Size: 5627 KasI G'GCGC_C Cuts at: 3057 3305 3057 Size: 248 5379 KpnI G_GTAC'C Cuts at: 2647 2647 Size: 5627 MluI A'CGCG_T Cuts at: 5060 5060 Size: 5627 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 273 551 2333 4298 4482 273 Size: 278 1782 1965 184 1418 Fragments arranged by size: 1965 1782 1418 278 184 MscI TGG'CCA Cuts at: 2473 3388 2473 Size: 915 4712 MslI CAynn'nnrTG Cuts at: 1015 1066 1372 1768 1969 2061 3616 3743 1015 Size: 51 306 396 201 92 1555 127 2899 Fragments arranged by size: 2899 1555 396 306 201 127 92 51 MspA1I CmG'CkG Cuts at: 813 985 1632 2361 2558 2738 3052 3412 4425 Size: 172 647 729 197 180 314 360 1013 Cuts at: 4425 4670 813 Size: 245 1770 Fragments arranged by size: 1770 1013 729 647 360 314 245 197 180 172 NaeI GCC'GGC Cuts at: 3808 3808 Size: 5627 NarI GG'CG_CC Cuts at: 3058 3306 3058 Size: 248 5379 NcoI C'CATG_G Cuts at: 107 2258 2381 107 Size: 2151 123 3353 Fragments arranged by size: 3353 2151 123 NdeI CA'TA_TG Cuts at: 1769 1769 Size: 5627 NgoMIV G'CCGG_C Cuts at: 3806 3806 Size: 5627 NspI r_CATG'y Cuts at: 687 1974 2680 3180 4087 687 Size: 1287 706 500 907 2227 Fragments arranged by size: 2227 1287 907 706 500 PciI A'CATG_T Cuts at: 1970 4083 1970 Size: 2113 3514 PflMI CCAn_nnn'nTGG Cuts at: 1996 1996 Size: 5627 PfoI T'CCnGG_A Cuts at: 473 1561 473 Size: 1088 4539 PmeI GTTT'AAAC Cuts at: 2626 2626 Size: 5627 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4766 4798 4766 Size: 32 5595 PshAI GACnn'nnGTC Cuts at: 1358 1358 Size: 5627 PsiI TTA'TAA Cuts at: 1589 1589 Size: 5627 PspOMI G'GGCC_C Cuts at: 2336 2336 Size: 5627 PstI C_TGCA'G Cuts at: 980 2674 3359 980 Size: 1694 685 3248 Fragments arranged by size: 3248 1694 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5627 PvuII CAG'CTG Cuts at: 813 985 1632 2361 3052 3412 813 Size: 172 647 729 691 360 3028 Fragments arranged by size: 3028 729 691 647 360 172 RsrII CG'GwC_CG Cuts at: 2170 3822 2170 Size: 1652 3975 SacI G_AGCT'C Cuts at: 2641 2641 Size: 5627 SalI G'TCGA_C Cuts at: 2664 2664 Size: 5627 SapI GCTCTTCn'nnn_ Cuts at: 2404 3650 3860 2404 Size: 1246 210 4171 Fragments arranged by size: 4171 1246 210 SbfI CC_TGCA'GG Cuts at: 2674 2674 Size: 5627 ScaI AGT'ACT Cuts at: 2290 5144 2290 Size: 2854 2773 SfcI C'TryA_G Cuts at: 33 157 976 1627 2553 2670 3355 4348 4539 Size: 124 819 651 926 117 685 993 191 Cuts at: 4539 33 Size: 1121 Fragments arranged by size: 1121 993 926 819 685 651 191 124 117 SfoI GGC'GCC Cuts at: 3059 3307 3059 Size: 248 5379 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5627 SgrAI Cr'CCGG_yG Cuts at: 352 352 Size: 5627 SmaI CCC'GGG Cuts at: 382 2649 382 Size: 2267 3360 SmlI C'TyrA_G Cuts at: 2782 3697 4189 4451 2782 Size: 915 492 262 3958 Fragments arranged by size: 3958 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5627 SphI G_CATG'C Cuts at: 687 2680 3180 687 Size: 1993 500 3134 Fragments arranged by size: 3134 1993 500 StyI C'CwwG_G Cuts at: 107 801 1755 2071 2258 2381 2761 3115 107 Size: 694 954 316 187 123 380 354 2619 Fragments arranged by size: 2619 954 694 380 354 316 187 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 499 1348 1477 2187 2233 3210 3343 3802 4011 Size: 849 129 710 46 977 133 459 209 Cuts at: 4011 499 Size: 2115 Fragments arranged by size: 2115 977 849 710 459 209 133 129 46 TatI w'GTAC_w Cuts at: 1502 2288 5142 1502 Size: 786 2854 1987 Fragments arranged by size: 2854 1987 786 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1147 1179 2414 2446 5144 5176 1147 Size: 32 1235 32 2698 32 1598 Fragments arranged by size: 2698 1598 1235 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3424 3424 Size: 5627 XbaI T'CTAG_A Cuts at: 2658 2658 Size: 5627 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1774 2466 3122 1774 Size: 692 656 4279 Fragments arranged by size: 4279 692 656 XmaI C'CCGG_G Cuts at: 380 2647 380 Size: 2267 3360 XmnI GAAnn'nnTTC Cuts at: 244 2548 244 Size: 2304 3323 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BssHII BstEII BstXI Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI ZraI