(Linear) (Six-base) MAPSORT of: KIEE2585.seq Check: 3523 from: 1 to: 2514 KIEE2585.orf With 182 enzymes: SgfI * September 4, 2015 15:51 .. AccI GT'mk_AC Cuts at: 0 1201 2514 Size: 1201 1313 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 373 1511 2514 Size: 343 30 1138 1003 Fragments arranged by size: 1138 1003 343 30 AflIII A'CryG_T Cuts at: 0 1862 2514 Size: 1862 652 AgeI A'CCGG_T Cuts at: 0 244 2455 2514 Size: 244 2211 59 Fragments arranged by size: 2211 244 59 AleI CACnn'nnGTG Cuts at: 0 1953 2514 Size: 1953 561 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 893 2514 Size: 893 1621 AlwNI CAG_nnn'CTG Cuts at: 0 61 1888 2514 Size: 61 1827 626 Fragments arranged by size: 1827 626 61 ApaI G_GGCC'C Cuts at: 0 2232 2514 Size: 2232 282 ApaLI G'TGCA_C Cuts at: 0 664 2514 Size: 664 1850 ApoI r'AATT_y Cuts at: 0 148 385 1922 2440 2514 Size: 148 237 1537 518 74 Fragments arranged by size: 1537 518 237 148 74 AvaI C'yCGr_G Cuts at: 0 272 2514 Size: 272 2242 BamHI G'GATC_C Cuts at: 0 450 642 1307 2514 Size: 450 192 665 1207 Fragments arranged by size: 1207 665 450 192 BanI G'GyrC_C Cuts at: 0 992 2514 Size: 992 1522 BanII G_rGCy'C Cuts at: 0 2232 2514 Size: 2232 282 BbvCI CC'TCA_GC Cuts at: 0 2233 2514 Size: 2233 281 BclI T'GATC_A Cuts at: 0 2375 2514 Size: 2375 139 Bme1580I G_kGCm'C Cuts at: 0 668 997 1042 2232 2514 Size: 668 329 45 1190 282 Fragments arranged by size: 1190 668 329 282 45 BmrI ACTGGGnnnn_n' Cuts at: 0 2430 2514 Size: 2430 84 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 766 2406 2514 Size: 766 1640 108 Fragments arranged by size: 1640 766 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 818 1476 1562 2514 Size: 658 160 658 86 952 Fragments arranged by size: 952 658 658 160 86 Bpu10I CC'TnA_GC Cuts at: 0 2233 2293 2514 Size: 2233 60 221 Fragments arranged by size: 2233 221 60 BsaAI yAC'GTr Cuts at: 0 663 2514 Size: 663 1851 BsaBI GATnn'nnATC Cuts at: 0 2284 2514 Size: 2284 230 BsaWI w'CCGG_w Cuts at: 0 14 244 2455 2514 Size: 14 230 2211 59 Fragments arranged by size: 2211 230 59 14 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 997 1027 2089 2119 2194 2224 2306 2336 Size: 997 30 1062 30 75 30 82 30 Cuts at: 2336 2514 Size: 178 Fragments arranged by size: 1062 997 178 82 75 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2025 2086 2514 Size: 2025 61 428 Fragments arranged by size: 2025 428 61 BseYI C'CCAG_C Cuts at: 0 38 1530 2514 Size: 38 1492 984 Fragments arranged by size: 1492 984 38 BsiEI CG_ry'CG Cuts at: 0 1468 2514 Size: 1468 1046 BsiHKAI G_wGCw'C Cuts at: 0 668 2514 Size: 668 1846 BsmI GAATG_Cn' Cuts at: 0 1836 2514 Size: 1836 678 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1489 2514 Size: 1489 1025 Bsp1286I G_dGCh'C Cuts at: 0 668 997 1042 2232 2514 Size: 668 329 45 1190 282 Fragments arranged by size: 1190 668 329 282 45 BspEI T'CCGG_A Cuts at: 0 14 2514 Size: 14 2500 BspHI T'CATG_A Cuts at: 0 1754 2514 Size: 1754 760 BsrDI GCAATG_nn' Cuts at: 0 1589 2514 Size: 1589 925 BsrFI r'CCGG_y Cuts at: 0 244 2455 2514 Size: 244 2211 59 Fragments arranged by size: 2211 244 59 BsrGI T'GTAC_A Cuts at: 0 1394 2514 Size: 1394 1120 BstYI r'GATC_y Cuts at: 0 450 642 1307 2514 Size: 450 192 665 1207 Fragments arranged by size: 1207 665 450 192 BstZ17I GTA'TAC Cuts at: 0 1202 2514 Size: 1202 1312 BtgI C'CryG_G Cuts at: 0 2150 2273 2360 2514 Size: 2150 123 87 154 Fragments arranged by size: 2150 154 123 87 BtsI GCAGTG_nn' Cuts at: 0 2204 2339 2407 2514 Size: 2204 135 68 107 Fragments arranged by size: 2204 135 107 68 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2022 2057 2514 Size: 2022 35 457 Fragments arranged by size: 2022 457 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2152 2514 Size: 2152 362 EaeI y'GGCC_r Cuts at: 0 1465 2363 2514 Size: 1465 898 151 Fragments arranged by size: 1465 898 151 EagI C'GGCC_G Cuts at: 0 1465 2514 Size: 1465 1049 EarI CTCTTCn'nnn_ Cuts at: 0 349 461 498 1487 2296 2504 2514 Size: 349 112 37 989 809 208 10 Fragments arranged by size: 989 809 349 208 112 37 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 463 2514 Size: 463 2051 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 373 658 818 1476 1511 1562 2514 Size: 343 30 285 160 658 35 51 952 Fragments arranged by size: 952 658 343 285 160 51 35 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 613 2514 Size: 613 1901 EcoO109I rG'GnC_Cy Cuts at: 0 2229 2385 2514 Size: 2229 156 129 Fragments arranged by size: 2229 156 129 EcoRI G'AATT_C Cuts at: 0 1922 2514 Size: 1922 592 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 378 1516 2514 Size: 378 1138 998 Fragments arranged by size: 1138 998 378 FspI TGC'GCA Cuts at: 0 2249 2514 Size: 2249 265 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 435 460 467 492 804 836 997 1029 Size: 435 25 7 25 312 32 161 32 Cuts at: 1029 2089 2121 2514 Size: 1060 32 393 Fragments arranged by size: 1060 435 393 312 161 32 32 32 25 25 7 HindIII A'AGCT_T Cuts at: 0 190 2514 Size: 190 2324 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 165 443 2225 2514 Size: 165 278 1782 289 Fragments arranged by size: 1782 289 278 165 MscI TGG'CCA Cuts at: 0 2365 2514 Size: 2365 149 MslI CAynn'nnrTG Cuts at: 0 907 958 1264 1660 1861 1953 2514 Size: 907 51 306 396 201 92 561 Fragments arranged by size: 907 561 396 306 201 92 51 MspA1I CmG'CkG Cuts at: 0 705 877 1524 2253 2450 2514 Size: 705 172 647 729 197 64 Fragments arranged by size: 729 705 647 197 172 64 NcoI C'CATG_G Cuts at: 0 2150 2273 2514 Size: 2150 123 241 Fragments arranged by size: 2150 241 123 NdeI CA'TA_TG Cuts at: 0 1661 2514 Size: 1661 853 NspI r_CATG'y Cuts at: 0 579 1866 2514 Size: 579 1287 648 Fragments arranged by size: 1287 648 579 PciI A'CATG_T Cuts at: 0 1862 2514 Size: 1862 652 PflMI CCAn_nnn'nTGG Cuts at: 0 1888 2514 Size: 1888 626 PfoI T'CCnGG_A Cuts at: 0 365 1453 2514 Size: 365 1088 1061 Fragments arranged by size: 1088 1061 365 PshAI GACnn'nnGTC Cuts at: 0 1250 2514 Size: 1250 1264 PsiI TTA'TAA Cuts at: 0 1481 2514 Size: 1481 1033 PspOMI G'GGCC_C Cuts at: 0 2228 2514 Size: 2228 286 PstI C_TGCA'G Cuts at: 0 872 2514 Size: 872 1642 PvuII CAG'CTG Cuts at: 0 705 877 1524 2253 2514 Size: 705 172 647 729 261 Fragments arranged by size: 729 705 647 261 172 RsrII CG'GwC_CG Cuts at: 0 2062 2514 Size: 2062 452 SapI GCTCTTCn'nnn_ Cuts at: 0 2296 2514 Size: 2296 218 ScaI AGT'ACT Cuts at: 0 2182 2514 Size: 2182 332 SfcI C'TryA_G Cuts at: 0 49 868 1519 2445 2514 Size: 49 819 651 926 69 Fragments arranged by size: 926 819 651 69 49 SgrAI Cr'CCGG_yG Cuts at: 0 244 2514 Size: 244 2270 SmaI CCC'GGG Cuts at: 0 274 2514 Size: 274 2240 SphI G_CATG'C Cuts at: 0 579 2514 Size: 579 1935 StyI C'CwwG_G Cuts at: 0 693 1647 1963 2150 2273 2514 Size: 693 954 316 187 123 241 Fragments arranged by size: 954 693 316 241 187 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 391 1240 1369 2079 2125 2514 Size: 391 849 129 710 46 389 Fragments arranged by size: 849 710 391 389 129 46 TatI w'GTAC_w Cuts at: 0 1394 2180 2514 Size: 1394 786 334 Fragments arranged by size: 1394 786 334 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1039 1071 2306 2338 2514 Size: 1039 32 1235 32 176 Fragments arranged by size: 1235 1039 176 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1666 2358 2514 Size: 1666 692 156 Fragments arranged by size: 1666 692 156 XmaI C'CCGG_G Cuts at: 0 272 2514 Size: 272 2242 XmnI GAAnn'nnTTC Cuts at: 0 136 2440 2514 Size: 136 2304 74 Fragments arranged by size: 2304 136 74 Enzymes that do cut: AccI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbvCI BclI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BstYI BstZ17I BtgI BtsI CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HindIII MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI PshAI PsiI PspOMI PstI PvuII RsrII SapI ScaI SfcI SgrAI SmaI SphI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AhdI AloI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaHI BsgI BsiWI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI DraI DraIII EcoICRI EcoRV FseI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PspXI PsrI PvuI SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI