################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI AspEI BbeI BbrPI BfrI BsaAI BsaHI BsiEI BsiWI BsmBI Bst1107I BstBI BstZ17I CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI Hin1I HpaI KasI KspI MluI NarI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SfoI SfuI SgrAI SnaBI SwaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 766 GGTACC G^GTACC Asp700I 2130 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 766 GGTACC G^GTACC AvrII 1213 CCTAGG C^CTAGG BciVI 4965 GGATAC GTATCC(6/5) BlnI 1213 CCTAGG C^CTAGG BsaBI 3687 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BssHII 3844 GCGCGC G^CGCGC BstEII 1326 GGTCACC G^GT[ACGT]ACC BstPI 1326 GGTCACC G^GT[ACGT]ACC BstXI 4907 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 2014 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1326 GGTCACC G^GT[ACGT]ACC EcoT22I 1078 ATGCAT ATGCA^T HgaI 4197 GCGTC GACGC(5/10) KpnI 766 GGTACC GGTAC^C MamI 3687 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 593 CAATTG C^AATTG MunI 593 CAATTG C^AATTG NaeI 1780 GCCGGC GCC^GGC NcoI 2060 CCATGG C^CATGG NgoMIV 1780 GCCGGC G^CCGGC NsiI 1078 ATGCAT ATGCA^T PaeR7I 3915 CTCGAG C^TCGAG PshAI 105 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 3039 CCTGCAGG CCTGCA^GG SexAI 5609 ACCTGGT A^CC[AT]GGT SpeI 3900 ACTAGT A^CTAGT SphI 3597 GCATGC GCATG^C Sse8387I 3039 CCTGCAGG CCTGCA^GG StuI 692 AGGCCT AGG^CCT XhoI 3915 CTCGAG C^TCGAG XmnI 2130 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 1300 GTCTAC GT^[AC][GT]AC 5528 GTCTAC AccII 3760 CGCG CG^CG 3845 CGCG AccIII 3143 TCCGGA T^CCGGA 4994 TCCGGA AflIII 1484 ACATGT A^C[GA][CT]GT 4218 ACATGT Aor51HI 323 AGCGCT AGC^GCT 3416 AGCGCT AviII 3533 TGCGCA TGC^GCA 4688 TGCGCA BamHI 755 GGATCC G^GATCC 5705 GGATCC BcgI 3696 CGATGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5105 GCAGGAAAATCG BsaI 3182 GAGACC GGTCTC(1/5) 4871 GAGACC BseAI 3143 TCCGGA T^CCGGA 4994 TCCGGA BsmI 4233 GAATGC GAATGC(1/-1) 4467 GAATGC Bsp1407I 308 TGTACA T^GTACA 1268 TGTACA BspDI 2218 ATCGAT AT^CGAT 3694 ATCGAT BspEI 3143 TCCGGA T^CCGGA 4994 TCCGGA BspHI 2474 TCATGA T^CATGA 4295 TCATGA BspLU11I 1484 ACATGT A^CATGT 4218 ACATGT BsrFI 1780 GCCGGC [GA]^CCGG[CT] 3469 GCCGGT BsrGI 308 TGTACA T^GTACA 1268 TGTACA BssSI 292 CACGAG CACGAG(-5/-1) 5713 CACGAG BstAPI 589 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1276 GCAGTGGGTGC BstUI 3760 CGCG CG^CG 3845 CGCG Cfr10I 1780 GCCGGC [GA]^CCGG[CT] 3469 GCCGGT ClaI 2218 ATCGAT AT^CGAT 3694 ATCGAT Eco47III 323 AGCGCT AGC^GCT 3416 AGCGCT FspI 3533 TGCGCA TGC^GCA 4688 TGCGCA HindIII 3247 AAGCTT A^AGCTT 4415 AAGCTT MaeII 1534 ACGT A^CGT 5681 ACGT MroI 3143 TCCGGA T^CCGGA 4994 TCCGGA MvnI 3760 CGCG CG^CG 3845 CGCG RcaI 2474 TCATGA T^CATGA 4295 TCATGA SacI 1573 GAGCTC GAGCT^C 5125 GAGCTC SapI 2593 GAAGAGC GCTCTTC(1/4) 5778 GAAGAGC ScaI 581 AGTACT AGT^ACT 1370 AGTACT SfiI 3390 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5274 GGCCAAGAAGGCC SmaI 3395 CCCGGG CCC^GGG 3431 CCCGGG SspBI 308 TGTACA T^GTACA 1268 TGTACA SspI 3869 AATATT AAT^ATT 5590 AATATT XmaCI 3395 CCCGGG C^CCGGG 3431 CCCGGG XmaI 3395 CCCGGG C^CCGGG 3431 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoII