Restriction map for
pF1KE2664
(Circular) (Six-base) MAPSORT of: pF1KE2664.seq Check: 7940 from: 1 to: 3545
pF1KE2664 3545 bp
With 182 enzymes: SgfI *
January 12, 2017 11:32 ..
AccI GT'mk_AC
Cuts at: 583 583
Size: 3545
Acc65I G'GTAC_C
Cuts at: 561 561
Size: 3545
AclI AA'CG_TT
Cuts at: 3385 3385
Size: 3545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1370 1802 2549 2817 193
Size: 1177 432 747 268 921
Fragments arranged by size:
1177 921 747 432 268
AflIII A'CryG_T
Cuts at: 897 2001 2978 897
Size: 1104 977 1464
Fragments arranged by size:
1464 1104 977
AgeI A'CCGG_T
Cuts at: 1933 1933
Size: 3545
AlwNI CAG_nnn'CTG
Cuts at: 165 850 2417 165
Size: 685 1567 1293
Fragments arranged by size:
1567 1293 685
ApaLI G'TGCA_C
Cuts at: 2315 2315
Size: 3545
ApoI r'AATT_y
Cuts at: 549 2898 3154 549
Size: 2349 256 940
Fragments arranged by size:
2349 940 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3545
AvaI C'yCGr_G
Cuts at: 565 565
Size: 3545
BamHI G'GATC_C
Cuts at: 570 570
Size: 3545
BanI G'GyrC_C
Cuts at: 561 975 1223 1258 561
Size: 414 248 35 2848
Fragments arranged by size:
2848 414 248 35
BanII G_rGCy'C
Cuts at: 152 559 1589 152
Size: 407 1030 2108
Fragments arranged by size:
2108 1030 407
BbeI G_GCGC'C
Cuts at: 979 1227 979
Size: 248 3297
BbsI GAAGACnn'nnnn_
Cuts at: 269 269
Size: 3545
BciVI GTATCCnnnnn_n'
Cuts at: 1443 2204 3545 1443
Size: 761 1341 1443
Fragments arranged by size:
1443 1341 761
BclI T'GATC_A
Cuts at: 1065 1065
Size: 3545
BglII A'GATC_T
Cuts at: 1060 3097 1060
Size: 2037 1508
BlpI GC'TnA_GC
Cuts at: 657 657
Size: 3545
Bme1580I G_kGCm'C
Cuts at: 211 270 484 1170 1263 2319 211
Size: 59 214 686 93 1056 1437
Fragments arranged by size:
1437 1056 686 214 93 59
BmrI ACTGGGnnnn_n'
Cuts at: 487 757 838 1173 3317 487
Size: 270 81 335 2144 715
Fragments arranged by size:
2144 715 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1912 1912
Size: 3545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 446
Size: 3545
Bpu10I CC'TnA_GC
Cuts at: 166 166
Size: 3545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1600 2092 2390 721
Size: 879 492 298 1876
Fragments arranged by size:
1876 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 516 3249 516
Size: 2733 812
BsaAI yAC'GTr
Cuts at: 898 898
Size: 3545
BsaBI GATnn'nnATC
Cuts at: 6 376 1059 3096 6
Size: 370 683 2037 455
Fragments arranged by size:
2037 683 455 370
BsaHI Gr'CG_yC
Cuts at: 976 1224 1926 3422 976
Size: 248 702 1496 1099
Fragments arranged by size:
1496 1099 702 248
BsaWI w'CCGG_w
Cuts at: 734 1255 1933 2207 2354 2765 734
Size: 521 678 274 147 411 1514
Fragments arranged by size:
1514 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 970 1728 2305 2801 970
Size: 758 577 496 1714
Fragments arranged by size:
1714 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 434
Size: 3545
BsiEI CG_ry'CG
Cuts at: 104 146 1087 1133 2341 104
Size: 42 941 46 1208 1308
Fragments arranged by size:
1308 1208 941 46 42
BsiHKAI G_wGCw'C
Cuts at: 559 1337 1527 2319 559
Size: 778 190 792 1785
Fragments arranged by size:
1785 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 211 270 484 559 1170 1263 1337 1527
Size: 59 59 214 75 611 93 74 190
Cuts at: 1527 1589 2319 152
Size: 62 730 1378
Fragments arranged by size:
1378 730 611 214 190 93 75 74
62 59 59
BspEI T'CCGG_A
Cuts at: 2765 2765
Size: 3545
BspHI T'CATG_A
Cuts at: 2721 2721
Size: 3545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 595 809 1111 1492 595
Size: 214 302 381 2648
Fragments arranged by size:
2648 381 302 214
BsrBI CCG'CTC
Cuts at: 50 125 1837 1891 3375 3540 50
Size: 75 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 165 75 55 54
BsrDI GCAATG_nn'
Cuts at: 1457 1457
Size: 3545
BsrFI r'CCGG_y
Cuts at: 1543 1724 1933 1543
Size: 181 209 3155
Fragments arranged by size:
3155 209 181
BssSI C'ACGA_G
Cuts at: 1816 2174 1816
Size: 358 3187
BstAPI GCAn_nnn'nTGC
Cuts at: 421 922 421
Size: 501 3044
BstBI TT'CG_AA
Cuts at: 1906 1906
Size: 3545
BstXI CCAn_nnnn'nTGG
Cuts at: 245 245
Size: 3545
BstYI r'GATC_y
Cuts at: 1 161 570 1037 1060 1395 1641 2653 2739
Size: 160 409 467 23 335 246 1012 86
Cuts at: 2739 2751 3097 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 409 346 335 246 160
86 23 12
BtgI C'CryG_G
Cuts at: 107 184 107
Size: 77 3468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1495 1676 1839 1495
Size: 181 163 3201
Fragments arranged by size:
3201 181 163
BtsI GCAGTG_nn'
Cuts at: 788 788
Size: 3545
DraI TTT'AAA
Cuts at: 544 544
Size: 3545
DrdI GACnn_nn'nnGTC
Cuts at: 1251 2109 1251
Size: 858 2687
EaeI y'GGCC_r
Cuts at: 143 1130 1304 1695 1722 3067 143
Size: 987 174 391 27 1345 621
Fragments arranged by size:
1345 987 621 391 174 27
EagI C'GGCC_G
Cuts at: 143 1130 143
Size: 987 2558
EarI CTCTTCn'nnn_
Cuts at: 289 322 1568 1778 289
Size: 33 1246 210 2056
Fragments arranged by size:
2056 1246 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1753 2063 2209 3355 1753
Size: 310 146 1146 1943
Fragments arranged by size:
1943 1146 310 146
EcoICRI GAG'CTC
Cuts at: 557 557
Size: 3545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 446 1370 1802 2549 2817 193
Size: 253 924 432 747 268 921
Fragments arranged by size:
924 921 747 432 268 253
EcoO109I rG'GnC_Cy
Cuts at: 200 684 200
Size: 484 3061
EcoRI G'AATT_C
Cuts at: 549 549
Size: 3545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1564 1564
Size: 3545
FspI TGC'GCA
Cuts at: 1326 1326
Size: 3545
HaeII r_GCGC'y
Cuts at: 979 1227 2249 3228 979
Size: 248 1022 979 1296
Fragments arranged by size:
1296 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1387 1417 1419 1449 1387
Size: 30 2 30 3483
Fragments arranged by size:
3483 30 30 2
HincII GTy'rAC
Cuts at: 584 584
Size: 3545
KasI G'GCGC_C
Cuts at: 975 1223 975
Size: 248 3297
KpnI G_GTAC'C
Cuts at: 565 565
Size: 3545
MluI A'CGCG_T
Cuts at: 2978 2978
Size: 3545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2400 2216
Size: 184 3361
MscI TGG'CCA
Cuts at: 1306 1306
Size: 3545
MslI CAynn'nnrTG
Cuts at: 338 1534 1661 338
Size: 1196 127 2222
Fragments arranged by size:
2222 1196 127
MspA1I CmG'CkG
Cuts at: 134 186 656 970 1330 2343 2588 134
Size: 52 470 314 360 1013 245 1091
Fragments arranged by size:
1091 1013 470 360 314 245 52
NaeI GCC'GGC
Cuts at: 1726 1726
Size: 3545
NarI GG'CG_CC
Cuts at: 976 1224 976
Size: 248 3297
NcoI C'CATG_G
Cuts at: 107 107
Size: 3545
NgoMIV G'CCGG_C
Cuts at: 1724 1724
Size: 3545
NspI r_CATG'y
Cuts at: 598 1098 2005 598
Size: 500 907 2138
Fragments arranged by size:
2138 907 500
PciI A'CATG_T
Cuts at: 2001 2001
Size: 3545
PmeI GTTT'AAAC
Cuts at: 544 544
Size: 3545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2684 2716 2684
Size: 32 3513
PpuMI rG'GwC_Cy
Cuts at: 200 200
Size: 3545
PstI C_TGCA'G
Cuts at: 158 592 1277 158
Size: 434 685 2426
Fragments arranged by size:
2426 685 434
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3545
PvuII CAG'CTG
Cuts at: 970 1330 970
Size: 360 3185
RsrII CG'GwC_CG
Cuts at: 1740 1740
Size: 3545
SacI G_AGCT'C
Cuts at: 559 559
Size: 3545
SacII CC_GC'GG
Cuts at: 187 187
Size: 3545
SalI G'TCGA_C
Cuts at: 582 582
Size: 3545
SanDI GG'GwC_CC
Cuts at: 200 200
Size: 3545
SapI GCTCTTCn'nnn_
Cuts at: 1568 1778 1568
Size: 210 3335
SbfI CC_TGCA'GG
Cuts at: 592 592
Size: 3545
ScaI AGT'ACT
Cuts at: 3062 3062
Size: 3545
SfcI C'TryA_G
Cuts at: 33 154 588 1273 2266 2457 33
Size: 121 434 685 993 191 1121
Fragments arranged by size:
1121 993 685 434 191 121
SfoI GGC'GCC
Cuts at: 977 1225 977
Size: 248 3297
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3545
SmaI CCC'GGG
Cuts at: 567 567
Size: 3545
SmlI C'TyrA_G
Cuts at: 700 1615 2107 2369 700
Size: 915 492 262 1876
Fragments arranged by size:
1876 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3545
SphI G_CATG'C
Cuts at: 598 1098 598
Size: 500 3045
StyI C'CwwG_G
Cuts at: 107 679 1033 107
Size: 572 354 2619
Fragments arranged by size:
2619 572 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1128 1261 1720 1929 1128
Size: 133 459 209 2744
Fragments arranged by size:
2744 459 209 133
TatI w'GTAC_w
Cuts at: 3060 3060
Size: 3545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3513
Tth111I GACn'n_nGTC
Cuts at: 1342 1342
Size: 3545
XbaI T'CTAG_A
Cuts at: 576 576
Size: 3545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1040 1040
Size: 3545
XmaI C'CCGG_G
Cuts at: 565 565
Size: 3545
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI