Restriction map for pF1KE2393
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MspA1I      MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          385  GTCTAC               GT^[AC][GT]AC
AcyI          558  GACGCC               G[GA]^CG[CT]C
Alw44I        955  GTGCAC               G^TGCAC
AocI          306  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1001  AGCGCT               AGC^GCT
ApaI         1471  GGGCCC               GGGCC^C
ApaLI         955  GTGCAC               G^TGCAC
AspI          544  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1108  CCCGAG               C^[CT]CG[GA]G
BamHI         311  GGATCC               G^GATCC
BanI          987  GGCACC               G^G[CT][GA]CC
BpmI         1196  CTCCAG               CTGGAG(16/14)
BsaHI         558  GACGCC               G[GA]^CG[CT]C
BsaWI         404  TCCGGT               [AT]^CCGG[AT]
BseRI         147  CTCCTC               GAGGAG(10/8)
BsmAI        1194  GTCTC                GTCTC(1/5)
BsoBI        1108  CCCGAG               C^[CT]CG[GA]G
BspMI         764  ACCTGC               ACCTGC(4/8)
BstEII        915  GGTCACC              G^GT[ACGT]ACC
BstPI         915  GGTCACC              G^GT[ACGT]ACC
BstYI         311  GGATCC               [GA]^GATC[CT]
Bsu36I        306  CCTGAGG              CC^T[ACGT]AGG
DrdI         1330  GACCAGAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1001  AGCGCT               AGC^GCT
Eco81I        306  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1247  CCTCTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       915  GGTCACC              G^GT[ACGT]ACC
Hin1I         558  GACGCC               G[GA]^CG[CT]C
MflI          311  GGATCC               [GA]^GATC[CT]
NaeI         1201  GCCGGC               GCC^GGC
NgoMIV       1201  GCCGGC               G^CCGGC
PflFI         544  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         367  GGGACCT              [GA]G^G[AT]CC[CT]
SapI         1428  GCTCTTC              GCTCTTC(1/4)
ScaI          473  AGTACT               AGT^ACT
SmlI          505  CTCAAG               C^T[CT][GA]AG
Tth111I       544  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          563  CCACAGCCAGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         311  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwNI         936  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1460  CAGCTTCTG           
BalI           68  TGGCCA               TGG^CCA
              746  TGGCCA              
BanII        1426  GGGCTC               G[GA]GC[CT]^C
             1471  GGGCCC              
BbsI         1327  GAAGAC               GAAGAC(2/6)
              757  GTCTTC              
BcnI         1065  CCCGG                CC^[GC]GG
             1212  CCCGG               
BpuAI        1327  GAAGAC               GAAGAC(2/6)
              757  GTCTTC              
BsaAI         655  TACGTG               [CT]AC^GT[GA]
             1357  TACGTG              
BsmFI         367  GGGAC                GGGAC(10/14)
              602  GGGAC               
BsrBI         513  CCGCTC               CCGCTC(-3/-3)
              963  CCGCTC              
BsrFI        1201  GCCGGC               [GA]^CCGG[CT]
             1376  ACCGGC              
BsrI         1349  ACTGG                ACTGG(1/-1)
              590  CCAGT               
BssHII          6  GCGCGC               G^CGCGC
                8  GCGCGC              
BstAPI       1014  GCACAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1088  GCAAGTCATGC         
Cfr10I       1201  GCCGGC               [GA]^CCGG[CT]
             1376  ACCGGC              
DsaI          905  CCATGG               C^C[GA][CT]GG
              983  CCATGG              
EcoT14I       905  CCATGG               C^C[AT][AT]GG
              983  CCATGG              
MluNI          68  TGGCCA               TGG^CCA
              746  TGGCCA              
MscI           68  TGGCCA               TGG^CCA
              746  TGGCCA              
NciI         1065  CCCGG                CC^[GC]GG
             1212  CCCGG               
NcoI          905  CCATGG               C^CATGG
              983  CCATGG              
PflMI         230  CCAATGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              978  CCACACCATGG         
PstI          172  CTGCAG               CTGCA^G
             1181  CTGCAG              
SfaNI         326  GCATC                GCATC(5/9)
              800  GCATC               
SfcI          172  CTGCAG               C^T[GA][CT]AG
             1181  CTGCAG              
StyI          905  CCATGG               C^C[AT][AT]GG
              983  CCATGG              
TaqI          485  TCGA                 T^CGA
              869  TCGA                
TthHB8I       485  TCGA                 T^CGA
              869  TCGA                
Van91I        230  CCAATGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              978  CCACACCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaII       BbvI        BmyI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        Bsp1286I    
BspHI       BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       TseI        Tsp45I      TspRI       
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