Restriction map for pF1KE2393
 (Linear) (Six-base) MAPSORT of: KIEE2393.seq  Check: 6578  from: 1  to: 1494



KIEE2393.orf

 With 182 enzymes: SgfI * 

                        January 26, 2012 13:39  ..

AccI GT'mk_AC

Cuts at:      0     386    1494 
   Size:        386    1108
 
AfeI AGC'GCT

Cuts at:      0    1003    1494 
   Size:       1003     491
 
AleI CACnn'nnGTG

Cuts at:      0     907    1494 
   Size:        907     587
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     349     823    1494 
   Size:        349     474     671

  Fragments arranged by size: 

                 671     474     349
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1366    1398    1494 
   Size:       1366      32      96

  Fragments arranged by size: 

                1366      96      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     941    1465    1494 
   Size:        941     524      29

  Fragments arranged by size: 

                 941     524      29
 
ApaI G_GGCC'C

Cuts at:      0    1475    1494 
   Size:       1475      19
 
ApaLI G'TGCA_C

Cuts at:      0     955    1494 
   Size:        955     539
 
AvaI C'yCGr_G

Cuts at:      0    1108    1494 
   Size:       1108     386
 
BamHI G'GATC_C

Cuts at:      0     311    1494 
   Size:        311    1183
 
BanI G'GyrC_C

Cuts at:      0     987    1494 
   Size:        987     507
 
BanII G_rGCy'C

Cuts at:      0    1430    1475    1494 
   Size:       1430      45      19

  Fragments arranged by size: 

                1430      45      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     750    1334    1494 
   Size:        750     584     160

  Fragments arranged by size: 

                 750     584     160
 
Bme1580I G_kGCm'C

Cuts at:      0     959    1420    1475    1494 
   Size:        959     461      55      19

  Fragments arranged by size: 

                 959     461      55      19
 
BmgBI CAC'GTC

Cuts at:      0     663    1494 
   Size:        663     831
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     584    1494 
   Size:        584     910
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1181    1494 
   Size:       1181     313
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490    1494 
   Size:        490    1004
 
BsaAI yAC'GTr

Cuts at:      0     657    1359    1494 
   Size:        657     702     135

  Fragments arranged by size: 

                 702     657     135
 
BsaHI Gr'CG_yC

Cuts at:      0     559    1494 
   Size:        559     935
 
BsaWI w'CCGG_w

Cuts at:      0     404    1494 
   Size:        404    1090
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     138    1494 
   Size:        138    1356
 
BseYI C'CCAG_C

Cuts at:      0     135     188     649     970    1084    1304    1484    1494 
   Size:        135      53     461     321     114     220     180      10

  Fragments arranged by size: 

                 461     321     220     180     135     114      53      10
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     483     997    1291    1494 
   Size:        483     514     294     203

  Fragments arranged by size: 

                 514     483     294     203
 
BsiHKAI G_wGCw'C

Cuts at:      0     734     950     959    1494 
   Size:        734     216       9     535

  Fragments arranged by size: 

                 734     535     216       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     734     950     959    1420    1430    1475    1494 
   Size:        734     216       9     461      10      45      19

  Fragments arranged by size: 

                 734     461     216      45      19      10       9
 
BspHI T'CATG_A

Cuts at:      0     221     554    1037    1494 
   Size:        221     333     483     457

  Fragments arranged by size: 

                 483     457     333     221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     773    1494 
   Size:        773     721
 
BsrBI CCG'CTC

Cuts at:      0     515     965    1494 
   Size:        515     450     529

  Fragments arranged by size: 

                 529     515     450
 
BsrFI r'CCGG_y

Cuts at:      0    1201    1376    1494 
   Size:       1201     175     118

  Fragments arranged by size: 

                1201     175     118
 
BssHII G'CGCG_C

Cuts at:      0       6       8    1494 
   Size:          6       2    1486

  Fragments arranged by size: 

                1486       6       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1020    1094    1494 
   Size:       1020      74     400

  Fragments arranged by size: 

                1020     400      74
 
BstEII G'GTnAC_C

Cuts at:      0     915    1494 
   Size:        915     579
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     657     744     912    1494 
   Size:        657      87     168     582

  Fragments arranged by size: 

                 657     582     168      87
 
BstYI r'GATC_y

Cuts at:      0     311    1494 
   Size:        311    1183
 
Bsu36I CC'TnA_GG

Cuts at:      0     307    1494 
   Size:        307    1187
 
BtgI C'CryG_G

Cuts at:      0     905     983    1494 
   Size:        905      78     511

  Fragments arranged by size: 

                 905     511      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1284    1494 
   Size:       1284     210
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1336    1494 
   Size:       1336     158
 
EaeI y'GGCC_r

Cuts at:      0      68     746    1067    1494 
   Size:         68     678     321     427

  Fragments arranged by size: 

                 678     427     321      68
 
EarI CTCTTCn'nnn_

Cuts at:      0    1039    1435    1450    1494 
   Size:       1039     396      15      44

  Fragments arranged by size: 

                1039     396      44      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     121    1494 
   Size:        121    1373
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1181    1494 
   Size:       1181     313
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1251    1494 
   Size:       1251     243
 
EcoO109I rG'GnC_Cy

Cuts at:      0     368    1471    1472    1494 
   Size:        368    1103       1      22

  Fragments arranged by size: 

                1103     368      22       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1023    1494 
   Size:       1023     471
 
HaeII r_GCGC'y

Cuts at:      0     455    1005    1047    1494 
   Size:        455     550      42     447

  Fragments arranged by size: 

                 550     455     447      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     203     235    1494 
   Size:        203      32    1259

  Fragments arranged by size: 

                1259     203      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     797    1163    1284    1494 
   Size:        434     363     366     121     210

  Fragments arranged by size: 

                 434     366     363     210     121
 
MscI TGG'CCA

Cuts at:      0      70     748    1494 
   Size:         70     678     746

  Fragments arranged by size: 

                 746     678      70
 
MslI CAynn'nnrTG

Cuts at:      0     805     907    1402    1494 
   Size:        805     102     495      92

  Fragments arranged by size: 

                 805     495     102      92
 
NaeI GCC'GGC

Cuts at:      0    1203    1494 
   Size:       1203     291
 
NcoI C'CATG_G

Cuts at:      0     905     983    1494 
   Size:        905      78     511

  Fragments arranged by size: 

                 905     511      78
 
NgoMIV G'CCGG_C

Cuts at:      0    1201    1494 
   Size:       1201     293
 
PasI CC'CwG_GG

Cuts at:      0     619     992    1226    1494 
   Size:        619     373     234     268

  Fragments arranged by size: 

                 619     373     268     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     236     984    1494 
   Size:        236     748     510

  Fragments arranged by size: 

                 748     510     236
 
PfoI T'CCnGG_A

Cuts at:      0     517    1494 
   Size:        517     977
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1162    1194    1494 
   Size:       1162      32     300

  Fragments arranged by size: 

                1162     300      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     368    1494 
   Size:        368    1126
 
PspOMI G'GGCC_C

Cuts at:      0    1471    1494 
   Size:       1471      23
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     883     915    1144    1176    1494 
   Size:        883      32     229      32     318

  Fragments arranged by size: 

                 883     318     229      32      32
 
PstI C_TGCA'G

Cuts at:      0     176    1185    1494 
   Size:        176    1009     309

  Fragments arranged by size: 

                1009     309     176
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1435    1494 
   Size:       1435      59
 
ScaI AGT'ACT

Cuts at:      0     475    1494 
   Size:        475    1019
 
SfcI C'TryA_G

Cuts at:      0     172    1181    1494 
   Size:        172    1009     313

  Fragments arranged by size: 

                1009     313     172
 
SmlI C'TyrA_G

Cuts at:      0     505    1494 
   Size:        505     989
 
StyI C'CwwG_G

Cuts at:      0     905     983    1494 
   Size:        905      78     511

  Fragments arranged by size: 

                 905     511      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     873     991    1380    1494 
   Size:        873     118     389     114

  Fragments arranged by size: 

                 873     389     118     114
 
TatI w'GTAC_w

Cuts at:      0     473    1160    1494 
   Size:        473     687     334

  Fragments arranged by size: 

                 687     473     334
 
Tth111I GACn'n_nGTC

Cuts at:      0     547    1494 
   Size:        547     947
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     570    1494 
   Size:        570     924
 

 Enzymes that do cut:

     AccI     AfeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbsI Bme1580I    BmgBI     BmrI
     BpmI    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     DrdI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     MmeI     MscI     MslI     NaeI     NcoI   NgoMIV     PasI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PsrI     PstI     SapI     ScaI
     SfcI     SmlI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI    BssSI    BstBI  BstZ17I
     BtsI     ClaI    CspCI     DraI   DraIII     EagI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NarI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI
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