################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MamI MfeI MflI MluI MroI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 227 GCGG CCGC(-3/-1) AocI 7 CCTGAGG CC^T[ACGT]AGG ApaI 747 GGGCCC GGGCC^C Asp700I 291 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 820 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 653 CTCGAG C^[CT]CG[GA]G BbsI 706 GTCTTC GAAGAC(2/6) BfaI 571 CTAG C^TAG BlpI 62 GCTTAGC GC^T[ACGT]AGC BpmI 543 CTCCAG CTGGAG(16/14) Bpu1102I 62 GCTTAGC GC^T[ACGT]AGC BpuAI 706 GTCTTC GAAGAC(2/6) BsaI 656 GAGACC GGTCTC(1/5) BseRI 514 CTCCTC GAGGAG(10/8) BsoBI 653 CTCGAG C^[CT]CG[GA]G BssKI 618 CCTGG ^CC[ACGT]GG BstNI 618 CCTGG CC^[AT]GG Bsu36I 7 CCTGAGG CC^T[ACGT]AGG CelII 62 GCTTAGC GC^T[ACGT]AGC DraII 747 GGGCCCT [GA]G^G[ACGT]CC[CT] EarI 54 GAAGAG CTCTTC(1/4) Eco81I 7 CCTGAGG CC^T[ACGT]AGG EcoO109I 747 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 618 CCTGG ^CC[AT]GG HindIII 30 AAGCTT A^AGCTT Ksp632I 54 GAAGAG CTCTTC(1/4) MaeI 571 CTAG C^TAG MaeIII 242 GTCAC ^GT[ACGT]AC MseI 830 TTAA T^TAA MslI 213 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 254 CAGCTG C[AC]G^C[GT]G MvaI 618 CCTGG CC^[AT]GG PaeR7I 653 CTCGAG C^TCGAG PflFI 820 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 324 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 254 CAGCTG CAG^CTG SapI 54 GAAGAGC GCTCTTC(1/4) ScrFI 618 CCTGG CC^[ACGT]GG SfcI 740 CTGTAG C^T[GA][CT]AG SmlI 653 CTCGAG C^T[CT][GA]AG TfiI 735 GATTC G^A[AT]TC Tru9I 830 TTAA T^TAA Tsp45I 242 GTCAC ^GT[GC]AC Tsp509I 302 AATT ^AATT Tth111I 820 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 324 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 653 CTCGAG C^TCGAG XmnI 291 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BalI 209 TGGCCA TGG^CCA 500 TGGCCA BsmFI 621 GGGAC GGGAC(10/14) 665 GTCCC BsrI 45 ACTGG ACTGG(1/-1) 324 CCAGT BstXI 212 CCATTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 545 CCAGAAACTTGG Cac8I 223 GCTGGC GC[ACGT]^[ACGT]GC 688 GCAGGC Cfr13I 747 GGGCC G^G[ACGT]CC 748 GGCCC DpnII 411 GATC ^GATC 490 GATC EaeI 209 TGGCCA [CT]^GGCC[GA] 500 TGGCCA EcoT14I 114 CCTTGG C^C[AT][AT]GG 683 CCTTGG MaeII 97 ACGT A^CGT 156 ACGT MboI 411 GATC ^GATC 490 GATC MboII 54 GAAGA GAAGA(8/7) 707 TCTTC MluNI 209 TGGCCA TGG^CCA 500 TGGCCA MscI 209 TGGCCA TGG^CCA 500 TGGCCA NdeII 411 GATC ^GATC 490 GATC PshAI 380 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 658 GACCTGAGTC Sau3AI 411 GATC ^GATC 490 GATC Sau96I 747 GGGCC G^G[ACGT]CC 748 GGCCC StuI 567 AGGCCT AGG^CCT 774 AGGCCT StyI 114 CCTTGG C^C[AT][AT]GG 683 CCTTGG TaqI 150 TCGA T^CGA 654 TCGA TthHB8I 150 TCGA T^CGA 654 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AlwNI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrDI BstF5I DdeI Fnu4HI FokI HaeIII HinfI ItaI MnlI NlaIII NlaIV PleI TseI TspRI