Restriction map for pF1KB8480
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AviII       AvrII       BbeI        BbrPI       
BcgI        BcnI        BfaI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BseAI       
BsiWI       BsoBI       Bsp1407I    BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       Cfr10I      
CpoI        DraI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     FseI        
FspI        HaeII       Hin1I       KasI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        173  GGTACC               G^GTACC
AflII        2301  CTTAAG               C^TTAAG
AocI          370  CCTGAGG              CC^T[ACGT]AGG
ApaI           40  GGGCCC               GGGCC^C
Asp700I      1891  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       173  GGTACC               G^GTACC
BalI          759  TGGCCA               TGG^CCA
BbvCI         857  CCTCAGC              CCTCAGC(-5/-2)
BciVI         642  GGATAC               GTATCC(6/5)
BfrI         2301  CTTAAG               C^TTAAG
BglI          427  GCCTTTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         491  AGATCT               A^GATCT
BpmI         2266  CTGGAG               CTGGAG(16/14)
BsaWI         542  TCCGGT               [AT]^CCGG[AT]
BsiEI          93  CGACCG               CG[GA][CT]^CG
BsmI          268  GCATTC               GAATGC(1/-1)
BspDI        1219  ATCGAT               AT^CGAT
BstAPI        436  GCAGAAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        370  CCTGAGG              CC^T[ACGT]AGG
Cac8I         732  GCAGGC               GC[ACGT]^[ACGT]GC
ClaI         1219  ATCGAT               AT^CGAT
DraII          40  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1397  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1983  GACTTTAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          759  TGGCCA               [CT]^GGCC[GA]
Eco81I        370  CCTGAGG              CC^T[ACGT]AGG
EcoNI         324  CCTCTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       40  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       527  ATGCAT               ATGCA^T
HapII         543  CCGG                 C^CGG
HgaI          283  GCGTC                GACGC(5/10)
HincII        166  GTTAAC               GT[CT]^[GA]AC
HindII        166  GTTAAC               GT[CT]^[GA]AC
HpaI          166  GTTAAC               GTT^AAC
HpaII         543  CCGG                 C^CGG
KpnI          173  GGTACC               GGTAC^C
MluNI         759  TGGCCA               TGG^CCA
MscI          759  TGGCCA               TGG^CCA
MspA1I       1960  CAGCTG               C[AC]G^C[GT]G
MspI          543  CCGG                 C^CGG
NdeI         2174  CATATG               CA^TATG
NsiI          527  ATGCAT               ATGCA^T
NspI         2415  ACATGC               [GA]CATG^[CT]
PstI          337  CTGCAG               CTGCA^G
PvuII        1960  CAGCTG               CAG^CTG
SapI         1228  GAAGAGC              GCTCTTC(1/4)
SexAI        1865  ACCAGGT              A^CC[AT]GGT
StuI         2007  AGGCCT               AGG^CCT
TfiI         2201  GAATC                G^A[AT]TC
XmnI         1891  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1531  GTAGAC               GT^[AC][GT]AC
             2557  GTAGAC              
AccII          89  CGCG                 CG^CG
               91  CGCG                
AlwNI          57  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2315  CAGAGGCTG           
BamHI         102  GGATCC               G^GATCC
              220  GGATCC              
BanI          159  GGCACC               G^G[CT][GA]CC
              173  GGTACC              
BsaI         1855  GAGACC               GGTCTC(1/5)
             2296  GAGACC              
BsmBI         261  CGTCTC               CGTCTC(1/5)
              418  GAGACG              
BspHI        2087  TCATGA               T^CATGA
             2204  TCATGA              
BstUI          89  CGCG                 CG^CG
               91  CGCG                
CfoI           90  GCGC                 GCG^C
             2374  GCGC                
EcoRI        1297  GAATTC               G^AATTC
             1372  GAATTC              
EcoRV         745  GATATC               GAT^ATC
             1010  GATATC              
HhaI           90  GCGC                 GCG^C
             2374  GCGC                
HinP1I         90  GCGC                 G^CGC
             2374  GCGC                
MvnI           89  CGCG                 CG^CG
               91  CGCG                
PflMI         393  CCAACCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1316  CCAATGACTGG         
RcaI         2087  TCATGA               T^CATGA
             2204  TCATGA              
SacI          505  GAGCTC               GAGCT^C
             1231  GAGCTC              
ScaI          953  AGTACT               AGT^ACT
             1946  AGTACT              
SfcI          337  CTGCAG               C^T[GA][CT]AG
             2430  CTACAG              
Van91I        393  CCAACCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1316  CCAATGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BclI        BmyI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeIII      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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