Restriction map for pF1KE2480
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       Alw44I      AocI        ApaI        ApaLI       
AscI        Asp718I     AviII       AvrII       BamHI       
BbeI        BbvCI       BcgI        BcnI        BfrI        
BglI        BlnI        BsaBI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BstBI       BstUI       Bsu36I      
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    FauI        FseI        FspI        KasI        
KpnI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PmeI        PpuMI       PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI         4286  GGCGTC               G[GA]^CG[CT]C
AhdI         1539  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       421  AGCGCT               AGC^GCT
Asp700I      3406  GAATCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1539  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1895  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3341  CTCGAG               C^[CT]CG[GA]G
BanI          884  GGCACC               G^G[CT][GA]CC
BanII        3338  GAGCTC               G[GA]GC[CT]^C
BclI            2  TGATCA               T^GATCA
BsaHI        4286  GGCGTC               G[GA]^CG[CT]C
BsmBI        4288  CGTCTC               CGTCTC(1/5)
BsmI         1016  GCATTC               GAATGC(1/-1)
BsoBI        3341  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1319  TGTACA               T^GTACA
BsrGI        1319  TGTACA               T^GTACA
BssSI        3655  CTCGTG               CACGAG(-5/-1)
Bst1107I     3689  GTATAC               GTA^TAC
BstEII       3040  GGTGACC              G^GT[ACGT]ACC
BstPI        3040  GGTGACC              G^GT[ACGT]ACC
BstXI         311  CCAGTGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      3689  GTATAC               GTA^TAC
CfoI          422  GCGC                 GCG^C
Eam1105I     1539  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      421  AGCGCT               AGC^GCT
EcoO65I      3040  GGTGACC              G^GT[ACGT]ACC
EcoRV        2892  GATATC               GAT^ATC
FbaI            2  TGATCA               T^GATCA
HaeII         421  AGCGCT               [GA]GCGC^[CT]
HgaI         4287  GCGTC                GACGC(5/10)
HhaI          422  GCGC                 GCG^C
Hin1I        4286  GGCGTC               G[GA]^CG[CT]C
HinP1I        422  GCGC                 G^CGC
HpaI         2742  GTTAAC               GTT^AAC
NspI         1177  GCATGT               [GA]CATG^[CT]
PaeR7I       3341  CTCGAG               C^TCGAG
PflFI        1895  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         841  CCAAAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        4162  CAGCTG               CAG^CTG
SacI         3338  GAGCTC               GAGCT^C
ScaI         1591  AGTACT               AGT^ACT
SexAI        1896  ACCTGGT              A^CC[AT]GGT
SspBI        1319  TGTACA               T^GTACA
Tth111I      1895  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        841  CCAAAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1572  TCTAGA               T^CTAGA
XhoI         3341  CTCGAG               C^TCGAG
XmnI         3406  GAATCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI          891  GTCTAC               GT^[AC][GT]AC
             3689  GTATAC              
AgeI          887  ACCGGT               A^CCGGT
             2938  ACCGGT              
AseI          922  ATTAAT               AT^TAAT
             2196  ATTAAT              
AsnI          922  ATTAAT               AT^TAAT
             2196  ATTAAT              
BbrPI        1907  CACGTG               CAC^GTG
             3114  CACGTG              
BciVI        2304  GGATAC               GTATCC(6/5)
             3528  GTATCC              
BglII        3849  AGATCT               A^GATCT
             3958  AGATCT              
BlpI         2232  GCTAAGC              GC^T[ACGT]AGC
             3500  GCTTAGC             
Bpu1102I     2232  GCTAAGC              GC^T[ACGT]AGC
             3500  GCTTAGC             
BsaAI        1907  CACGTG               [CT]AC^GT[GA]
             3114  CACGTG              
BsaI         4188  GAGACC               GGTCTC(1/5)
             3281  GGTCTC              
BsgI          928  GTGCAG               GTGCAG(16/14)
             2733  GTGCAG              
BsrDI        3201  CATTGC               GCAATG(2/0)
             4043  CATTGC              
BsrFI         887  ACCGGT               [GA]^CCGG[CT]
             2938  ACCGGT              
CelII        2232  GCTAAGC              GC^T[ACGT]AGC
             3500  GCTTAGC             
Cfr10I        887  ACCGGT               [GA]^CCGG[CT]
             2938  ACCGGT              
DsaI          587  CCATGG               C^C[GA][CT]GG
             4086  CCATGG              
EcoRI         870  GAATTC               G^AATTC
             1637  GAATTC              
EcoT22I       480  ATGCAT               ATGCA^T
             1310  ATGCAT              
HindIII       392  AAGCTT               A^AGCTT
             2235  AAGCTT              
MspA1I       3133  CAGCGG               C[AC]G^C[GT]G
             4162  CAGCTG              
NcoI          587  CCATGG               C^CATGG
             4086  CCATGG              
NsiI          480  ATGCAT               ATGCA^T
             1310  ATGCAT              
PinAI         887  ACCGGT               A^CCGGT
             2938  ACCGGT              
PmaCI        1907  CACGTG               CAC^GTG
             3114  CACGTG              
PmlI         1907  CACGTG               CAC^GTG
             3114  CACGTG              
PshBI         922  ATTAAT               AT^TAAT
             2196  ATTAAT              
PstI         1247  CTGCAG               CTGCA^G
             4327  CTGCAG              
SpeI          771  ACTAGT               A^CTAGT
             3880  ACTAGT              
SspI          295  AATATT               AAT^ATT
             1186  AATATT              
StuI          284  AGGCCT               AGG^CCT
             4280  AGGCCT              
TaqI         3143  TCGA                 T^CGA
             3342  TCGA                
TthHB8I      3143  TCGA                 T^CGA
             3342  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BalI        BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EaeI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MfeI        MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspI        MunI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       
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