Restriction map for pF1KE3218
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# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwNI       
Aor51HI     ApaI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsgI        BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRV       FbaI        FseI        FspI        HaeII       
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MscI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII        839  TCCGGA               T^CCGGA
AcsI          681  GAATTC               [GA]^AATT[CT]
Alw44I       1264  GTGCAC               G^TGCAC
AocI         1194  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1264  GTGCAC               G^TGCAC
ApoI          681  GAATTC               [GA]^AATT[CT]
AscI         1429  GGCGCGCC             GG^CGCGCC
AspHI        1264  GTGCAC               G[AT]GC[AT]^C
BanI          850  GGTGCC               G^G[CT][GA]CC
BanII        1419  GGGCTC               G[GA]GC[CT]^C
BbrPI        1231  CACGTG               CAC^GTG
BglI         1372  GCCTCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1231  CACGTG               [CT]AC^GT[GA]
BseAI         839  TCCGGA               T^CCGGA
BsiEI         513  CGACCG               CG[GA][CT]^CG
BsiHKAI      1264  GTGCAC               G[AT]GC[AT]^C
BsmBI         448  CGTCTC               CGTCTC(1/5)
BspEI         839  TCCGGA               T^CCGGA
BspHI        1104  TCATGA               T^CATGA
BssHII       1430  GCGCGC               G^CGCGC
BstAPI        824  GCATGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         482  TTCGAA               TT^CGAA
Bsu36I       1194  CCTGAGG              CC^T[ACGT]AGG
DraIII       1203  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          132  CCACGG               C^C[GA][CT]GG
EaeI          129  CGGCCA               [CT]^GGCC[GA]
EarI          556  CTCTTC               CTCTTC(1/4)
Eco81I       1194  CCTGAGG              CC^T[ACGT]AGG
EcoRI         681  GAATTC               G^AATTC
EcoT22I       371  ATGCAT               ATGCA^T
HgaI          447  GCGTC                GACGC(5/10)
Ksp632I       556  CTCTTC               CTCTTC(1/4)
MroI          839  TCCGGA               T^CCGGA
NsiI          371  ATGCAT               ATGCA^T
NspV          482  TTCGAA               TT^CGAA
PmaCI        1231  CACGTG               CAC^GTG
PmlI         1231  CACGTG               CAC^GTG
PstI          831  CTGCAG               CTGCA^G
RcaI         1104  TCATGA               T^CATGA
SapI          555  GCTCTTC              GCTCTTC(1/4)
SfuI          482  TTCGAA               TT^CGAA
SmlI          634  CTCAAG               C^T[CT][GA]AG
SphI          824  GCATGC               GCATG^C
TaqI          483  TCGA                 T^CGA
TthHB8I       483  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII        1409  CGCG                 CG^CG
             1431  CGCG                
AvaI          101  CCCGGG               C^[CT]CG[GA]G
             1424  CCCGGG              
BmyI         1264  GTGCAC               G[AGT]GC[ACT]^C
             1419  GGGCTC              
BsaWI         671  ACCGGA               [AT]^CCGG[AT]
              839  TCCGGA              
BseRI         180  CTCCTC               GAGGAG(10/8)
             1007  CTCCTC              
BsoBI         101  CCCGGG               C^[CT]CG[GA]G
             1424  CCCGGG              
Bsp1286I     1264  GTGCAC               G[AGT]GC[ACT]^C
             1419  GGGCTC              
BspMI         608  ACCTGC               ACCTGC(4/8)
             1250  ACCTGC              
BsrBI         116  CCGCTC               CCGCTC(-3/-3)
             1029  CCGCTC              
BsrI          531  ACTGG                ACTGG(1/-1)
             1391  CCAGT               
BssSI         439  CACGAG               CACGAG(-5/-1)
             1267  CACGAG              
BstUI        1409  CGCG                 CG^CG
             1431  CGCG                
CfoI         1430  GCGC                 GCG^C
             1432  GCGC                
HhaI         1430  GCGC                 GCG^C
             1432  GCGC                
HinP1I       1430  GCGC                 G^CGC
             1432  GCGC                
HincII        153  GTCAAC               GT[CT]^[GA]AC
              927  GTTGAC              
HindII        153  GTCAAC               GT[CT]^[GA]AC
              927  GTTGAC              
MslI          133  CACGGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1231  CACGTGAATG          
MvnI         1409  CGCG                 CG^CG
             1431  CGCG                
NspI          824  GCATGC               [GA]CATG^[CT]
             1260  GCATGT              
SfcI          831  CTGCAG               C^T[GA][CT]AG
             1479  CTACAG              
SmaI          101  CCCGGG               CCC^GGG
             1424  CCCGGG              
Tsp509I       421  AATT                 ^AATT
              682  AATT                
XmaCI         101  CCCGGG               C^CCGGG
             1424  CCCGGG              
XmaI          101  CCCGGG               C^CCGGG
             1424  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BamHI       BbsI        BbvI        BcnI        BpmI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsrFI       BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       XhoII       
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