Restriction map for pF1KE6355
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AcyI        AflII       AgeI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BcnI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FauI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MfeI        
MroI        MseI        MunI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tth111I     
TthHB8I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII          15  CGCG                 CG^CG
AcsI          925  AAATTC               [GA]^AATT[CT]
AfaI          414  GTAC                 GT^AC
AflIII         14  ACGCGT               A^C[GA][CT]GT
AhdI          752  GACGCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          925  AAATTC               [GA]^AATT[CT]
AspEI         752  GACGCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         995  GAGCTC               G[AT]GC[AT]^C
BanII         995  GAGCTC               G[GA]GC[CT]^C
BbvCI         351  CCTCAGC              CCTCAGC(-5/-2)
BclI          575  TGATCA               T^GATCA
BsaBI         573  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       995  GAGCTC               G[AT]GC[AT]^C
BsmAI         769  GAGAC                GTCTC(1/5)
Bsp1407I      413  TGTACA               T^GTACA
BspMI         370  ACCTGC               ACCTGC(4/8)
BsrDI         900  CATTGC               GCAATG(2/0)
BsrGI         413  TGTACA               T^GTACA
BstUI          15  CGCG                 CG^CG
BstYI         431  GGATCT               [GA]^GATC[CT]
DraII         443  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          134  CCATGG               C^C[GA][CT]GG
Eam1105I      752  GACGCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      443  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         480  GATATC               GAT^ATC
FbaI          575  TGATCA               T^GATCA
HaeII          59  AGCGCC               [GA]GCGC^[CT]
HapII         249  CCGG                 C^CGG
HinfI         933  GACTC                G^A[ACGT]TC
HpaII         249  CCGG                 C^CGG
MaeIII        604  GTCAC                ^GT[ACGT]AC
MamI          573  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          431  GGATCT               [GA]^GATC[CT]
MluI           14  ACGCGT               A^CGCGT
MspA1I        965  CAGCTG               C[AC]G^C[GT]G
MspI          249  CCGG                 C^CGG
MvnI           15  CGCG                 CG^CG
NcoI          134  CCATGG               C^CATGG
NspI          548  ACATGC               [GA]CATG^[CT]
PflMI         905  CCATATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          933  GACTC                GAGTC(4/5)
PpuMI         443  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         965  CAGCTG               CAG^CTG
RsaI          414  GTAC                 GT^AC
SacI          995  GAGCTC               GAGCT^C
SapI          633  GAAGAGC              GCTCTTC(1/4)
SspBI         413  TGTACA               T^GTACA
Tsp45I        604  GTCAC                ^GT[GC]AC
Van91I        905  CCATATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         431  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          431  GGATC                GGATC(4/5)
              518  GGATC               
Asp700I       886  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              982  GAAACAATTC          
BanI          459  GGTGCC               G^G[CT][GA]CC
             1009  GGCACC              
BbsI          244  GTCTTC               GAAGAC(2/6)
              319  GTCTTC              
BglI          171  GCCTAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              190  GCCCTGGTGGC         
BpmI          423  CTCCAG               CTGGAG(16/14)
              299  CTGGAG              
BpuAI         244  GTCTTC               GAAGAC(2/6)
              319  GTCTTC              
BsmFI         341  GGGAC                GGGAC(10/14)
              443  GGGAC               
Cac8I          56  GCAAGC               GC[ACGT]^[ACGT]GC
              180  GCTGGC              
CfoI           60  GCGC                 GCG^C
              552  GCGC                
DdeI           79  CTGAG                C^T[ACGT]AG
              352  CTCAG               
EarI          727  CTCTTC               CTCTTC(1/4)
              633  GAAGAG              
HhaI           60  GCGC                 GCG^C
              552  GCGC                
HinP1I         60  GCGC                 G^CGC
              552  GCGC                
HphI           35  TCACC                GGTGA(8/7)
              295  TCACC               
Ksp632I       727  CTCTTC               CTCTTC(1/4)
              633  GAAGAG              
Tsp509I       926  AATT                 ^AATT
              987  AATT                
XcmI          185  CCATCGCCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              803  CCATCCTCAATGTGG     
XmnI          886  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              982  GAAACAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AluI        AvaII       BalI        BbvI        BmyI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       Cfr13I      DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HgaI        
ItaI        MboI        MboII       MluNI       MnlI        
MscI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com