Restriction map for pF1KE6355
 (Linear) (Six-base) MAPSORT of: KIEE6355.seq  Check: 1993  from: 1  to: 1047



KIEE6355.orf

 With 182 enzymes: SgfI * 

                        July 14, 2018 20:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     379    1047 
   Size:        379     668
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     306     469     848     998    1047 
   Size:        274      32     163     379     150      49

  Fragments arranged by size: 

                 379     274     163     150      49      32
 
AflIII A'CryG_T

Cuts at:      0      14    1047 
   Size:         14    1033
 
AhdI GACnn_n'nnGTC

Cuts at:      0     757    1047 
   Size:        757     290
 
ApoI r'AATT_y

Cuts at:      0     925    1047 
   Size:        925     122
 
BanI G'GyrC_C

Cuts at:      0     459    1009    1047 
   Size:        459     550      38

  Fragments arranged by size: 

                 550     459      38
 
BanII G_rGCy'C

Cuts at:      0     999    1047 
   Size:        999      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     237     312    1047 
   Size:        237      75     735

  Fragments arranged by size: 

                 735     237      75
 
BbvCI CC'TCA_GC

Cuts at:      0     352    1047 
   Size:        352     695
 
BclI T'GATC_A

Cuts at:      0     575    1047 
   Size:        575     472
 
BglI GCCn_nnn'nGGC

Cuts at:      0     177     196    1047 
   Size:        177      19     851

  Fragments arranged by size: 

                 851     177      19
 
Bme1580I G_kGCm'C

Cuts at:      0     390     464    1047 
   Size:        390      74     583

  Fragments arranged by size: 

                 583     390      74
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1047 
   Size:       1047
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     408    1047 
   Size:        320      88     639

  Fragments arranged by size: 

                 639     320      88
 
Bpu10I CC'TnA_GC

Cuts at:      0     352    1047 
   Size:        352     695
 
BsaBI GATnn'nnATC

Cuts at:      0     577    1047 
   Size:        577     470
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     406     436    1047 
   Size:        406      30     611

  Fragments arranged by size: 

                 611     406      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     397     666     850     886    1047 
   Size:        397     269     184      36     161

  Fragments arranged by size: 

                 397     269     184     161      36
 
BseYI C'CCAG_C

Cuts at:      0     123     234     705    1047 
   Size:        123     111     471     342

  Fragments arranged by size: 

                 471     342     123     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     612     768    1047 
   Size:        114     498     156     279

  Fragments arranged by size: 

                 498     279     156     114
 
BsiHKAI G_wGCw'C

Cuts at:      0     999    1047 
   Size:        999      48
 
Bsp1286I G_dGCh'C

Cuts at:      0     390     464     999    1047 
   Size:        390      74     535      48

  Fragments arranged by size: 

                 535     390      74      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     379    1047 
   Size:        379     668
 
BsrDI GCAATG_nn'

Cuts at:      0     899    1047 
   Size:        899     148
 
BsrGI T'GTAC_A

Cuts at:      0     413    1047 
   Size:        413     634
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     192     627    1047 
   Size:         72     120     435     420

  Fragments arranged by size: 

                 435     420     120      72
 
BstYI r'GATC_y

Cuts at:      0     431    1047 
   Size:        431     616
 
BtgI C'CryG_G

Cuts at:      0     134    1047 
   Size:        134     913
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     171    1047 
   Size:        171     876
 
BtsI GCAGTG_nn'

Cuts at:      0     600    1047 
   Size:        600     447
 
EaeI y'GGCC_r

Cuts at:      0     182     272     329     538     599    1047 
   Size:        182      90      57     209      61     448

  Fragments arranged by size: 

                 448     209     182      90      61      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     628     733    1047 
   Size:        628     105     314

  Fragments arranged by size: 

                 628     314     105
 
EcoICRI GAG'CTC

Cuts at:      0     997    1047 
   Size:        997      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     306     320     408     469     848     998    1047 
   Size:        274      32      14      88      61     379     150      49

  Fragments arranged by size: 

                 379     274     150      88      61      49      32      14
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     444    1047 
   Size:        444     603
 
EcoRV GAT'ATC

Cuts at:      0     482    1047 
   Size:        482     565
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     177    1047 
   Size:        177     870
 
HaeII r_GCGC'y

Cuts at:      0      63    1047 
   Size:         63     984
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     141     173     560     592     743     775    1047 
   Size:        141      32     387      32     151      32     272

  Fragments arranged by size: 

                 387     272     151     141      32      32      32
 
MluI A'CGCG_T

Cuts at:      0      14    1047 
   Size:         14    1033
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     813    1047 
   Size:        813     234
 
MscI TGG'CCA

Cuts at:      0     184     274     331    1047 
   Size:        184      90      57     716

  Fragments arranged by size: 

                 716     184      90      57
 
MslI CAynn'nnrTG

Cuts at:      0      91     361     625    1047 
   Size:         91     270     264     422

  Fragments arranged by size: 

                 422     270     264      91
 
MspA1I CmG'CkG

Cuts at:      0     967    1047 
   Size:        967      80
 
NcoI C'CATG_G

Cuts at:      0     134    1047 
   Size:        134     913
 
NspI r_CATG'y

Cuts at:      0     552    1047 
   Size:        552     495
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     911    1047 
   Size:        911     136
 
PfoI T'CCnGG_A

Cuts at:      0     990    1047 
   Size:        990      57
 
PpuMI rG'GwC_Cy

Cuts at:      0     444    1047 
   Size:        444     603
 
PvuII CAG'CTG

Cuts at:      0     967    1047 
   Size:        967      80
 
SacI G_AGCT'C

Cuts at:      0     999    1047 
   Size:        999      48
 
SapI GCTCTTCn'nnn_

Cuts at:      0     628    1047 
   Size:        628     419
 
StyI C'CwwG_G

Cuts at:      0     134     390     938    1047 
   Size:        134     256     548     109

  Fragments arranged by size: 

                 548     256     134     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      60    1047 
   Size:         60     987
 
TatI w'GTAC_w

Cuts at:      0     413    1047 
   Size:        413     634
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     192     810    1047 
   Size:        192     618     237

  Fragments arranged by size: 

                 618     237     192
 
XmnI GAAnn'nnTTC

Cuts at:      0     890     986    1047 
   Size:        890      96      61

  Fragments arranged by size: 

                 890      96      61
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     ApoI     BanI    BanII     BbsI
    BbvCI     BclI     BglI Bme1580I     BplI     BpmI   Bpu10I    BsaBI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrGI    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NspI    PflMI     PfoI
    PpuMI    PvuII     SacI     SapI     StyI    TaqII     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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