Restriction map for pF1KE9612
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BanII       BbvCI       BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoRI       EcoT22I     FseI        FspI        HgaI        
HinfI       KpnI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          167  GTCTAC               GT^[AC][GT]AC
AcyI           34  GGCGCC               G[GA]^CG[CT]C
AlwNI         172  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          560  CCCGGG               C^[CT]CG[GA]G
BamHI         273  GGATCC               G^GATCC
BanI           34  GGCGCC               G^G[CT][GA]CC
BbeI           34  GGCGCC               GGCGC^C
BbrPI          46  CACGTG               CAC^GTG
BclI          339  TGATCA               T^GATCA
BsaAI          46  CACGTG               [CT]AC^GT[GA]
BsaHI          34  GGCGCC               G[GA]^CG[CT]C
BsmFI          23  GGGAC                GGGAC(10/14)
BsoBI         560  CCCGGG               C^[CT]CG[GA]G
BsrDI         719  CATTGC               GCAATG(2/0)
BsrI          117  ACTGG                ACTGG(1/-1)
BstEII        589  GGTGACC              G^GT[ACGT]ACC
BstF5I        657  CATCC                GGATG(2/0)
BstPI         589  GGTGACC              G^GT[ACGT]ACC
BstXI         263  CCACACCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         273  GGATCC               [GA]^GATC[CT]
Cac8I           7  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI           35  GCGC                 GCG^C
DraI           69  TTTAAA               TTT^AAA
DsaI          636  CCATGG               C^C[GA][CT]GG
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EarI          613  GAAGAG               CTCTTC(1/4)
EcoNI         300  CCTCATAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       589  GGTGACC              G^GT[ACGT]ACC
EcoRV         645  GATATC               GAT^ATC
EcoT14I       636  CCATGG               C^C[AT][AT]GG
FauI           20  GCGGG                CCCGC(4/6)
FbaI          339  TGATCA               T^GATCA
FokI          657  CATCC                GGATG(9/13)
HaeII          34  GGCGCC               [GA]GCGC^[CT]
HaeIII          3  GGCC                 GG^CC
HhaI           35  GCGC                 GCG^C
Hin1I          34  GGCGCC               G[GA]^CG[CT]C
HinP1I         35  GCGC                 G^CGC
HpaI          121  GTTAAC               GTT^AAC
HphI          589  GGTGA                GGTGA(8/7)
KasI           34  GGCGCC               G^GCGCC
Ksp632I       613  GAAGAG               CTCTTC(1/4)
MflI          273  GGATCC               [GA]^GATC[CT]
NarI           34  GGCGCC               GG^CGCC
NcoI          636  CCATGG               C^CATGG
PmaCI          46  CACGTG               CAC^GTG
PmlI           46  CACGTG               CAC^GTG
PstI          839  CTGCAG               CTGCA^G
PvuII         175  CAGCTG               CAG^CTG
SfoI           34  GGCGCC               GGC^GCC
SmaI          560  CCCGGG               CCC^GGG
SmlI          400  CTTGAG               C^T[CT][GA]AG
StyI          636  CCATGG               C^C[AT][AT]GG
TspRI         603  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         273  GGATCC               [GA]^GATC[CT]
XmaCI         560  CCCGGG               C^CCGGG
XmaI          560  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI            5  CCGC                 CCGC(-3/-1)
               20  GCGG                
AfaI          471  GTAC                 GT^AC
              565  GTAC                
AflIII         47  ACGTGT               A^C[GA][CT]GT
              359  ACGTGT              
AlwI          274  GATCC                GGATC(4/5)
              273  GGATC               
BsaJI         560  CCCGGG               C^C[ACGT][ACGT]GG
              636  CCATGG              
BstNI          54  CCTGG                CC^[AT]GG
              394  CCTGG               
DdeI          788  CTAAG                C^T[ACGT]AG
              845  CTCAG               
DpnII         274  GATC                 ^GATC
              340  GATC                
EcoRII         54  CCTGG                ^CC[AT]GG
              394  CCTGG               
HapII          39  CCGG                 C^CGG
              561  CCGG                
HindIII       596  AAGCTT               A^AGCTT
              697  AAGCTT              
HpaII          39  CCGG                 C^CGG
              561  CCGG                
MaeII          47  ACGT                 A^CGT
              359  ACGT                
MboI          274  GATC                 ^GATC
              340  GATC                
MslI          264  CACACCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              488  CATTAAAGTG          
MspA1I          5  CCGCTG               C[AC]G^C[GT]G
              175  CAGCTG              
MspI           39  CCGG                 C^CGG
              561  CCGG                
MvaI           54  CCTGG                CC^[AT]GG
              394  CCTGG               
MwoI           11  GCGTGGACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              844  GCTCAGAAGGC         
NdeII         274  GATC                 ^GATC
              340  GATC                
NlaIII        418  CATG                 CATG^
              637  CATG                
RsaI          471  GTAC                 GT^AC
              565  GTAC                
Sau3AI        274  GATC                 ^GATC
              340  GATC                
SfaNI          76  GATGC                GCATC(5/9)
              374  GCATC               
SspI           65  AATATT               AAT^ATT
              461  AATATT              
Tsp45I        421  GTGAC                ^GT[GC]AC
              590  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BbsI        BbvI        BcnI        BpuAI       
BsiYI       BslI        BssKI       Fnu4HI      HincII      
HindII      ItaI        MaeIII      MboII       MnlI        
MseI        NciI        NlaIV       ScrFI       SfcI        
Tru9I       TseI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com