Restriction map for pF1KE9612
 (Linear) (Six-base) MAPSORT of: KIEE9612.seq  Check: 3201  from: 1  to: 936



KIEE9612.orf

 With 182 enzymes: SgfI * 

                        March 10, 2017 10:38  ..

AccI GT'mk_AC

Cuts at:      0     168     936 
   Size:        168     768
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     695     936 
   Size:        695     241
 
AflIII A'CryG_T

Cuts at:      0      47     359     936 
   Size:         47     312     577

  Fragments arranged by size: 

                 577     312      47
 
AleI CACnn'nnGTG

Cuts at:      0     268     936 
   Size:        268     668
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     936 
   Size:        397     539
 
AlwNI CAG_nnn'CTG

Cuts at:      0     177     936 
   Size:        177     759
 
AvaI C'yCGr_G

Cuts at:      0     560     936 
   Size:        560     376
 
BamHI G'GATC_C

Cuts at:      0     273     936 
   Size:        273     663
 
BanI G'GyrC_C

Cuts at:      0      34     936 
   Size:         34     902
 
BbeI G_GCGC'C

Cuts at:      0      38     936 
   Size:         38     898
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     241     490     817     936 
   Size:        241     249     327     119

  Fragments arranged by size: 

                 327     249     241     119
 
BclI T'GATC_A

Cuts at:      0     339     936 
   Size:        339     597
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     268     936 
   Size:        268     668
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     936 
   Size:        421     515
 
BsaAI yAC'GTr

Cuts at:      0      48     936 
   Size:         48     888
 
BsaHI Gr'CG_yC

Cuts at:      0      35     936 
   Size:         35     901
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     936 
   Size:        310      30     596

  Fragments arranged by size: 

                 596     310      30
 
BsrDI GCAATG_nn'

Cuts at:      0     718     936 
   Size:        718     218
 
BstEII G'GTnAC_C

Cuts at:      0     589     936 
   Size:        589     347
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270     936 
   Size:        270     666
 
BstYI r'GATC_y

Cuts at:      0     273     936 
   Size:        273     663
 
BtgI C'CryG_G

Cuts at:      0     636     936 
   Size:        636     300
 
DraI TTT'AAA

Cuts at:      0      71     936 
   Size:         71     865
 
EaeI y'GGCC_r

Cuts at:      0       2     936 
   Size:          2     934
 
EarI CTCTTCn'nnn_

Cuts at:      0     608     936 
   Size:        608     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     695     936 
   Size:        695     241
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     304     936 
   Size:        304     632
 
EcoRV GAT'ATC

Cuts at:      0     647     936 
   Size:        647     289
 
HaeII r_GCGC'y

Cuts at:      0      38     936 
   Size:         38     898
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     667     699     828     860     936 
   Size:        667      32     129      32      76

  Fragments arranged by size: 

                 667     129      76      32      32
 
HincII GTy'rAC

Cuts at:      0     123     572     867     936 
   Size:        123     449     295      69

  Fragments arranged by size: 

                 449     295     123      69
 
HindIII A'AGCT_T

Cuts at:      0     596     697     936 
   Size:        596     101     239

  Fragments arranged by size: 

                 596     239     101
 
HpaI GTT'AAC

Cuts at:      0     123     936 
   Size:        123     813
 
KasI G'GCGC_C

Cuts at:      0      34     936 
   Size:         34     902
 
MslI CAynn'nnrTG

Cuts at:      0     268     492     936 
   Size:        268     224     444

  Fragments arranged by size: 

                 444     268     224
 
MspA1I CmG'CkG

Cuts at:      0       7     177     936 
   Size:          7     170     759

  Fragments arranged by size: 

                 759     170       7
 
NarI GG'CG_CC

Cuts at:      0      35     936 
   Size:         35     901
 
NcoI C'CATG_G

Cuts at:      0     636     936 
   Size:        636     300
 
PfoI T'CCnGG_A

Cuts at:      0     393     936 
   Size:        393     543
 
PmlI CAC'GTG

Cuts at:      0      48     936 
   Size:         48     888
 
PsiI TTA'TAA

Cuts at:      0     223     936 
   Size:        223     713
 
PstI C_TGCA'G

Cuts at:      0     843     936 
   Size:        843      93
 
PvuII CAG'CTG

Cuts at:      0     177     936 
   Size:        177     759
 
SfcI C'TryA_G

Cuts at:      0     169     764     839     936 
   Size:        169     595      75      97

  Fragments arranged by size: 

                 595     169      97      75
 
SfoI GGC'GCC

Cuts at:      0      36     936 
   Size:         36     900
 
SmaI CCC'GGG

Cuts at:      0     562     936 
   Size:        562     374
 
SmlI C'TyrA_G

Cuts at:      0     400     936 
   Size:        400     536
 
SspI AAT'ATT

Cuts at:      0      67     463     936 
   Size:         67     396     473

  Fragments arranged by size: 

                 473     396      67
 
StyI C'CwwG_G

Cuts at:      0     636     936 
   Size:        636     300
 
TatI w'GTAC_w

Cuts at:      0     470     936 
   Size:        470     466
 
XmaI C'CCGG_G

Cuts at:      0     560     936 
   Size:        560     376
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     AvaI    BamHI
     BanI     BbeI     BbsI     BclI     BplI    BpuEI    BsaAI    BsaHI
    BsaXI    BsrDI   BstEII    BstXI    BstYI     BtgI     DraI     EaeI
     EarI  Eco57MI    EcoNI    EcoRV    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     MslI   MspA1I     NarI     NcoI     PfoI     PmlI
     PsiI     PstI    PvuII     SfcI     SfoI     SmaI     SmlI     SspI
     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI
    BsaBI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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