Restriction map for pF1KE2630
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AgeI        Alw44I      ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AvrII       BamHI       BanII       
BbeI        BcgI        BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BsiWI       BsoBI       BspDI       
BspEI       BspHI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
EcoO65I     EcoRV       EcoT22I     FseI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AclI         1071  AACGTT               AA^CGTT
AhdI         2365  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2317  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       933  AGCGCT               AGC^GCT
Asp700I      1068  GAAAACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2365  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1648  GAGCAC               G[AT]GC[AT]^C
AviII         220  TGCGCA               TGC^GCA
BanI           84  GGTGCC               G^G[CT][GA]CC
BbrPI         373  CACGTG               CAC^GTG
BbsI          484  GTCTTC               GAAGAC(2/6)
BclI         2078  TGATCA               T^GATCA
BcnI          143  CCCGG                CC^[GC]GG
BpuAI         484  GTCTTC               GAAGAC(2/6)
BsiEI        2052  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1648  GAGCAC               G[AT]GC[AT]^C
BsmBI        1849  CGTCTC               CGTCTC(1/5)
BsmI         2229  GAATGC               GAATGC(1/-1)
Bsp1407I     1319  TGTACA               T^GTACA
BspLU11I      411  ACATGT               A^CATGT
BsrBI        1687  GAGCGG               CCGCTC(-3/-3)
BsrDI        1693  GCAATG               GCAATG(2/0)
BsrFI        1873  ACCGGC               [GA]^CCGG[CT]
BsrGI        1319  TGTACA               T^GTACA
BstAPI       2028  GCAGTACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        2058  AGATCC               [GA]^GATC[CT]
Bsu36I       2317  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1873  ACCGGC               [GA]^CCGG[CT]
DrdI          121  GACTGCTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2052  CGGCCG               C^GGCCG
Eam1105I     2365  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2052  CGGCCG               C^GGCCG
Eco47III      933  AGCGCT               AGC^GCT
Eco52I       2052  CGGCCG               C^GGCCG
Eco81I       2317  CCTGAGG              CC^T[ACGT]AGG
EcoNI         162  CCTATCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2247  GAATTC               G^AATTC
FauI         1689  GCGGG                CCCGC(4/6)
FbaI         2078  TGATCA               T^GATCA
FspI          220  TGCGCA               TGC^GCA
HgaI          686  GACGC                GACGC(5/10)
HincII        988  GTCAAC               GT[CT]^[GA]AC
HindII        988  GTCAAC               GT[CT]^[GA]AC
MflI         2058  AGATCC               [GA]^GATC[CT]
NciI          143  CCCGG                CC^[GC]GG
NcoI         1055  CCATGG               C^CATGG
NdeI         2162  CATATG               CA^TATG
NspI          411  ACATGT               [GA]CATG^[CT]
PmaCI         373  CACGTG               CAC^GTG
PmlI          373  CACGTG               CAC^GTG
Psp1406I     1071  AACGTT               AA^CGTT
SexAI        1958  ACCAGGT              A^CC[AT]GGT
SspBI        1319  TGTACA               T^GTACA
SspI         2270  AATATT               AAT^ATT
StuI          808  AGGCCT               AGG^CCT
XcmI         1762  CCAGCCGAGTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        2058  AGATCC               [GA]^GATC[CT]
XmnI         1068  GAAAACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AflIII        411  ACATGT               A^C[GA][CT]GT
              878  ACGTGT              
BbvCI        1364  CCTCAGC              CCTCAGC(-5/-2)
             1981  GCTGAGG             
BglI           16  GCCTTCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              898  GCCACAAAGGC         
BmyI         1648  GAGCAC               G[AGT]GC[ACT]^C
             2009  GTGCCC              
BsaAI         373  CACGTG               [CT]AC^GT[GA]
              877  TACGTG              
BsaI          379  GAGACC               GGTCTC(1/5)
              982  GAGACC              
BseRI         198  CTCCTC               GAGGAG(10/8)
             1602  CTCCTC              
Bsp1286I     1648  GAGCAC               G[AGT]GC[ACT]^C
             2009  GTGCCC              
BspMI        2033  ACCTGC               ACCTGC(4/8)
             1416  GCAGGT              
HapII         144  CCGG                 C^CGG
             1874  CCGG                
HpaII         144  CCGG                 C^CGG
             1874  CCGG                
MseI         2084  TTAA                 T^TAA
             2173  TTAA                
MspI          144  CCGG                 C^CGG
             1874  CCGG                
SapI          954  GAAGAGC              GCTCTTC(1/4)
             2179  GAAGAGC             
SfcI          704  CTACAG               C^T[GA][CT]AG
             2279  CTATAG              
Tru9I        2084  TTAA                 T^TAA
             2173  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BalI        BbvI        
BfaI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      
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