(Linear) (Six-base) MAPSORT of: KIEE2627.seq Check: 3361 from: 1 to: 813 KIEE2627.orf With 182 enzymes: SgfI * May 31, 2016 13:40 .. Acc65I G'GTAC_C Cuts at: 0 802 813 Size: 802 11 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 813 Size: 730 83 AlwNI CAG_nnn'CTG Cuts at: 0 111 174 282 792 813 Size: 111 63 108 510 21 Fragments arranged by size: 510 111 108 63 21 ApaI G_GGCC'C Cuts at: 0 195 813 Size: 195 618 ApoI r'AATT_y Cuts at: 0 589 813 Size: 589 224 AvaI C'yCGr_G Cuts at: 0 468 813 Size: 468 345 BamHI G'GATC_C Cuts at: 0 524 813 Size: 524 289 BanI G'GyrC_C Cuts at: 0 157 472 631 802 813 Size: 157 315 159 171 11 Fragments arranged by size: 315 171 159 157 11 BanII G_rGCy'C Cuts at: 0 11 195 404 646 813 Size: 11 184 209 242 167 Fragments arranged by size: 242 209 184 167 11 BbeI G_GCGC'C Cuts at: 0 635 813 Size: 635 178 BbsI GAAGACnn'nnnn_ Cuts at: 0 63 600 813 Size: 63 537 213 Fragments arranged by size: 537 213 63 BglI GCCn_nnn'nGGC Cuts at: 0 230 813 Size: 230 583 Bme1580I G_kGCm'C Cuts at: 0 195 475 813 Size: 195 280 338 Fragments arranged by size: 338 280 195 BmgBI CAC'GTC Cuts at: 0 240 813 Size: 240 573 BmrI ACTGGGnnnn_n' Cuts at: 0 612 813 Size: 612 201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 30 795 813 Size: 30 765 18 Fragments arranged by size: 765 30 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 554 739 813 Size: 12 542 185 74 Fragments arranged by size: 542 185 74 12 BsaAI yAC'GTr Cuts at: 0 267 663 813 Size: 267 396 150 Fragments arranged by size: 396 267 150 BsaHI Gr'CG_yC Cuts at: 0 632 813 Size: 632 181 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 52 640 754 813 Size: 52 588 114 59 Fragments arranged by size: 588 114 59 52 BseYI C'CCAG_C Cuts at: 0 275 282 813 Size: 275 7 531 Fragments arranged by size: 531 275 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 813 Size: 760 53 BsiHKAI G_wGCw'C Cuts at: 0 11 404 428 813 Size: 11 393 24 385 Fragments arranged by size: 393 385 24 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 246 813 Size: 246 567 Bsp1286I G_dGCh'C Cuts at: 0 11 195 404 428 475 646 813 Size: 11 184 209 24 47 171 167 Fragments arranged by size: 209 184 171 167 47 24 11 BsrBI CCG'CTC Cuts at: 0 14 304 560 813 Size: 14 290 256 253 Fragments arranged by size: 290 256 253 14 BsrFI r'CCGG_y Cuts at: 0 535 813 Size: 535 278 BstAPI GCAn_nnn'nTGC Cuts at: 0 423 792 813 Size: 423 369 21 Fragments arranged by size: 423 369 21 BstXI CCAn_nnnn'nTGG Cuts at: 0 520 813 Size: 520 293 BstYI r'GATC_y Cuts at: 0 524 813 Size: 524 289 BtgI C'CryG_G Cuts at: 0 134 336 456 796 813 Size: 134 202 120 340 17 Fragments arranged by size: 340 202 134 120 17 BtsI GCAGTG_nn' Cuts at: 0 412 710 813 Size: 412 298 103 Fragments arranged by size: 412 298 103 DrdI GACnn_nn'nnGTC Cuts at: 0 349 604 813 Size: 349 255 209 Fragments arranged by size: 349 255 209 EaeI y'GGCC_r Cuts at: 0 53 100 293 381 813 Size: 53 47 193 88 432 Fragments arranged by size: 432 193 88 53 47 EarI CTCTTCn'nnn_ Cuts at: 0 70 409 813 Size: 70 339 404 Fragments arranged by size: 404 339 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 662 813 Size: 662 151 EcoICRI GAG'CTC Cuts at: 0 9 402 813 Size: 9 393 411 Fragments arranged by size: 411 393 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 554 730 739 813 Size: 12 542 176 9 74 Fragments arranged by size: 542 176 74 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 27 166 813 Size: 27 139 647 Fragments arranged by size: 647 139 27 EcoO109I rG'GnC_Cy Cuts at: 0 108 191 342 714 813 Size: 108 83 151 372 99 Fragments arranged by size: 372 151 108 99 83 HaeII r_GCGC'y Cuts at: 0 456 635 699 813 Size: 456 179 64 114 Fragments arranged by size: 456 179 114 64 KasI G'GCGC_C Cuts at: 0 631 813 Size: 631 182 KpnI G_GTAC'C Cuts at: 0 806 813 Size: 806 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 671 813 Size: 671 142 MscI TGG'CCA Cuts at: 0 55 102 383 813 Size: 55 47 281 430 Fragments arranged by size: 430 281 55 47 MspA1I CmG'CkG Cuts at: 0 279 338 420 458 574 813 Size: 279 59 82 38 116 239 Fragments arranged by size: 279 239 116 82 59 38 NarI GG'CG_CC Cuts at: 0 632 813 Size: 632 181 NcoI C'CATG_G Cuts at: 0 134 813 Size: 134 679 PasI CC'CwG_GG Cuts at: 0 507 813 Size: 507 306 PflMI CCAn_nnn'nTGG Cuts at: 0 282 813 Size: 282 531 PpuMI rG'GwC_Cy Cuts at: 0 342 813 Size: 342 471 PspOMI G'GGCC_C Cuts at: 0 191 813 Size: 191 622 PstI C_TGCA'G Cuts at: 0 419 813 Size: 419 394 PvuII CAG'CTG Cuts at: 0 279 420 574 813 Size: 279 141 154 239 Fragments arranged by size: 279 239 154 141 SacI G_AGCT'C Cuts at: 0 11 404 813 Size: 11 393 409 Fragments arranged by size: 409 393 11 SacII CC_GC'GG Cuts at: 0 339 459 813 Size: 339 120 354 Fragments arranged by size: 354 339 120 SapI GCTCTTCn'nnn_ Cuts at: 0 409 813 Size: 409 404 SfcI C'TryA_G Cuts at: 0 124 415 813 Size: 124 291 398 Fragments arranged by size: 398 291 124 SfoI GGC'GCC Cuts at: 0 633 813 Size: 633 180 SmaI CCC'GGG Cuts at: 0 470 813 Size: 470 343 StyI C'CwwG_G Cuts at: 0 134 519 806 813 Size: 134 385 287 7 Fragments arranged by size: 385 287 134 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 611 643 813 Size: 611 32 170 Fragments arranged by size: 611 170 32 XmaI C'CCGG_G Cuts at: 0 468 813 Size: 468 345 Enzymes that do cut: Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BplI BpmI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI KpnI MmeI MscI MspA1I NarI NcoI PasI PflMI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI SmaI StyI TstI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI