Restriction map for pF1KE1136
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tru9I       TspRI       TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI         1000  GAATTC               [GA]^AATT[CT]
AfaI           48  GTAC                 GT^AC
AlwNI          11  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1000  GAATTC               [GA]^AATT[CT]
AspHI         273  GAGCAC               G[AT]GC[AT]^C
AspI          526  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          787  CCCGAG               C^[CT]CG[GA]G
AvaII         476  GGACC                G^G[AT]CC
BalI          101  TGGCCA               TGG^CCA
BamHI         305  GGATCC               G^GATCC
BbsI          523  GAAGAC               GAAGAC(2/6)
BbvCI         732  CCTCAGC              CCTCAGC(-5/-2)
BglI           26  GCCGCAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         523  GAAGAC               GAAGAC(2/6)
BsgI          166  GTGCAG               GTGCAG(16/14)
BsiHKAI       273  GAGCAC               G[AT]GC[AT]^C
BsoBI         787  CCCGAG               C^[CT]CG[GA]G
BspMI         713  GCAGGT               ACCTGC(4/8)
BsrBI         506  CCGCTC               CCGCTC(-3/-3)
BsrI          264  ACTGG                ACTGG(1/-1)
BssSI         862  CTCGTG               CACGAG(-5/-1)
BstXI         665  CCATCTGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         305  GGATCC               [GA]^GATC[CT]
CfoI          565  GCGC                 GCG^C
EcoNI         426  CCTAGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1000  GAATTC               G^AATTC
HaeII         564  GGCGCT               [GA]GCGC^[CT]
HhaI          565  GCGC                 GCG^C
HinP1I        565  GCGC                 G^CGC
HindIII        74  AAGCTT               A^AGCTT
MaeIII        118  GTGAC                ^GT[ACGT]AC
MflI          305  GGATCC               [GA]^GATC[CT]
MluNI         101  TGGCCA               TGG^CCA
MscI          101  TGGCCA               TGG^CCA
MslI          666  CATCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        973  CAGCTG               C[AC]G^C[GT]G
NcoI          821  CCATGG               C^CATGG
PflFI         526  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         752  CCAATGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          172  GACTC                GAGTC(4/5)
PshAI         477  GACCAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          976  CTGCAG               CTGCA^G
PvuII         973  CAGCTG               CAG^CTG
RsaI           48  GTAC                 GT^AC
SapI          870  GAAGAGC              GCTCTTC(1/4)
ScaI           47  AGTACT               AGT^ACT
TfiI          399  GATTC                G^A[AT]TC
Tsp45I        118  GTGAC                ^GT[GC]AC
Tsp509I      1001  AATT                 ^AATT
Tth111I       526  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        752  CCAATGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         305  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BcnI          110  CCGGG                CC^[GC]GG
              369  CCGGG               
BfaI           91  CTAG                 C^TAG
              427  CTAG                
BseRI         509  CTCCTC               GAGGAG(10/8)
              652  CTCCTC              
BsmAI         621  GAGAC                GTCTC(1/5)
              931  GAGAC               
Cac8I         332  GCCTGC               GC[ACGT]^[ACGT]GC
              336  GCTGGC              
DraII         279  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              628  GGGGCCC             
EcoO109I      279  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              628  GGGGCCC             
EcoT14I       743  CCTTGG               C^C[AT][AT]GG
              821  CCATGG              
HapII         110  CCGG                 C^CGG
              369  CCGG                
HinfI         172  GACTC                G^A[ACGT]TC
              399  GATTC               
HpaII         110  CCGG                 C^CGG
              369  CCGG                
MaeI           91  CTAG                 C^TAG
              427  CTAG                
MaeII         482  ACGT                 A^CGT
              548  ACGT                
MspI          110  CCGG                 C^CGG
              369  CCGG                
NciI          110  CCGGG                CC^[GC]GG
              369  CCGGG               
SfcI           51  CTACAG               C^T[GA][CT]AG
              976  CTGCAG              
SmlI          902  CTCAAG               C^T[CT][GA]AG
              917  CTCAAG              
StyI          743  CCTTGG               C^C[AT][AT]GG
              821  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        BanII       
BbvI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrDI       BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        
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