Restriction map for pF1KB6172
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HindIII     HphI        KasI        KpnI        
KspI        MamI        MluI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tsp45I      TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII        1461  GACGTC               GACGT^C
AccIII       1340  TCCGGA               T^CCGGA
AcyI         1461  GACGTC               G[GA]^CG[CT]C
AlwI          919  GATCC                GGATC(4/5)
AlwNI        1517  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI        1742  GTGCTC               G[AT]GC[AT]^C
AspI          184  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         592  GGACC                G^G[AT]CC
BalI          535  TGGCCA               TGG^CCA
BciVI         267  GTATCC               GTATCC(6/5)
BcnI          376  CCGGG                CC^[GC]GG
BmyI         1742  GTGCTC               G[AGT]GC[ACT]^C
BsaAI         102  TACGTA               [CT]AC^GT[GA]
BsaHI        1461  GACGTC               G[GA]^CG[CT]C
BsaWI        1340  TCCGGA               [AT]^CCGG[AT]
BseAI        1340  TCCGGA               T^CCGGA
BsiHKAI      1742  GTGCTC               G[AT]GC[AT]^C
BsiYI         210  CCTGATGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          210  CCTGATGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I     1742  GTGCTC               G[AGT]GC[ACT]^C
BspEI        1340  TCCGGA               T^CCGGA
BspHI        1124  TCATGA               T^CATGA
BspLU11I     1666  ACATGT               A^CATGT
BsrDI         493  GCAATG               GCAATG(2/0)
BssSI         152  CTCGTG               CACGAG(-5/-1)
BstF5I        990  CATCC                GGATG(2/0)
CfoI           47  GCGC                 GCG^C
DraI          775  TTTAAA               TTT^AAA
DraII         591  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          535  TGGCCA               [CT]^GGCC[GA]
EarI         1285  GAAGAG               CTCTTC(1/4)
EcoO109I      591  AGGACCT              [GA]G^G[ACGT]CC[CT]
FokI          990  CATCC                GGATG(9/13)
HaeII          46  AGCGCC               [GA]GCGC^[CT]
HhaI           47  GCGC                 GCG^C
Hin1I        1461  GACGTC               G[GA]^CG[CT]C
HinP1I         47  GCGC                 G^CGC
HincII        304  GTTAAC               GT[CT]^[GA]AC
HindII        304  GTTAAC               GT[CT]^[GA]AC
HpaI          304  GTTAAC               GTT^AAC
Ksp632I      1285  GAAGAG               CTCTTC(1/4)
MluNI         535  TGGCCA               TGG^CCA
MroI         1340  TCCGGA               T^CCGGA
MscI          535  TGGCCA               TGG^CCA
MslI          171  CACTGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1030  CAGCTG               C[AC]G^C[GT]G
MwoI          610  GCTTGCTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          376  CCGGG                CC^[GC]GG
NcoI          922  CCATGG               C^CATGG
PflFI         184  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         591  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          276  CTGCAG               CTGCA^G
PvuII        1030  CAGCTG               CAG^CTG
RcaI         1124  TCATGA               T^CATGA
ScaI          959  AGTACT               AGT^ACT
SfcI          276  CTGCAG               C^T[GA][CT]AG
SnaBI         102  TACGTA               TAC^GTA
SpeI         1233  ACTAGT               A^CTAGT
SphI          752  GCATGC               GCATG^C
SspI         1510  AATATT               AAT^ATT
Tth111I       184  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1086  CCATTTTGAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI           99  GTCTAC               GT^[AC][GT]AC
             1400  GTCTAC              
AflIII         53  ACACGT               A^C[GA][CT]GT
             1666  ACATGT              
BbsI         1102  GAAGAC               GAAGAC(2/6)
             1452  GAAGAC              
BglII         561  AGATCT               A^GATCT
             1620  AGATCT              
BpuAI        1102  GAAGAC               GAAGAC(2/6)
             1452  GAAGAC              
BseRI         233  GAGGAG               GAGGAG(10/8)
             1288  GAGGAG              
BstYI         561  AGATCT               [GA]^GATC[CT]
             1620  AGATCT              
Cfr13I        592  GGACC                G^G[ACGT]CC
              794  GGCCC               
DrdI          543  GACTTTTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1455  GACACAGACGTC        
EcoT14I       922  CCATGG               C^C[AT][AT]GG
              949  CCAAGG              
FauI          770  CCCGC                CCCGC(4/6)
              835  GCGGG               
MfeI          639  CAATTG               C^AATTG
             1639  CAATTG              
MflI          561  AGATCT               [GA]^GATC[CT]
             1620  AGATCT              
MunI          639  CAATTG               C^AATTG
             1639  CAATTG              
NlaIV         126  GGTTCC               GG[ACGT]^[ACGT]CC
              645  GGAACC              
Sau96I        592  GGACC                G^G[ACGT]CC
              794  GGCCC               
SfaNI         213  GATGC                GCATC(5/9)
             1117  GATGC               
StyI          922  CCATGG               C^C[AT][AT]GG
              949  CCAAGG              
XhoII         561  AGATCT               [GA]^GATC[CT]
             1620  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AseI        AsnI        BbvI        BfaI        BpmI        
BsaJI       BsmAI       BsrI        BssKI       BstNI       
Cac8I       DdeI        DpnII       DsaI        EcoRII      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspI        MvaI        
NdeII       NlaIII      NspI        PleI        PshBI       
RsaI        Sau3AI      ScrFI       TfiI        Tru9I       
TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com