Restriction map for
pF1KB6172
(Circular) (Six-base) MAPSORT of: pF1KB6172.seq Check: 7095 from: 1 to: 4925
pF1KB6172 4925 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AatII G_ACGT'C
Cuts at: 1573 1573
Size: 4925
AccI GT'mk_AC
Cuts at: 208 1509 1963 208
Size: 1301 454 3170
Fragments arranged by size:
3170 1301 454
Acc65I G'GTAC_C
Cuts at: 1941 1941
Size: 4925
AclI AA'CG_TT
Cuts at: 4765 4765
Size: 4925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1679 2750 3182 3929 4197 437
Size: 1242 1071 432 747 268 1165
Fragments arranged by size:
1242 1165 1071 747 432 268
AflIII A'CryG_T
Cuts at: 161 1774 2277 3381 4358 161
Size: 1613 503 1104 977 728
Fragments arranged by size:
1613 1104 977 728 503
AgeI A'CCGG_T
Cuts at: 3313 3313
Size: 4925
AleI CACnn'nnGTG
Cuts at: 283 283
Size: 4925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 887 887
Size: 4925
AlwNI CAG_nnn'CTG
Cuts at: 1630 2230 3797 1630
Size: 600 1567 2758
Fragments arranged by size:
2758 1567 600
ApaLI G'TGCA_C
Cuts at: 3695 3695
Size: 4925
ApoI r'AATT_y
Cuts at: 141 405 462 1929 4278 4534 141
Size: 264 57 1467 2349 256 532
Fragments arranged by size:
2349 1467 532 264 256 57
AseI AT'TA_AT
Cuts at: 20 1253 1444 1529 20
Size: 1233 191 85 3416
Fragments arranged by size:
3416 1233 191 85
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
AvaI C'yCGr_G
Cuts at: 1945 1945
Size: 4925
BamHI G'GATC_C
Cuts at: 1950 1950
Size: 4925
BanI G'GyrC_C
Cuts at: 1941 2355 2603 2638 1941
Size: 414 248 35 4228
Fragments arranged by size:
4228 414 248 35
BanII G_rGCy'C
Cuts at: 1939 2969 1939
Size: 1030 3895
BbeI G_GCGC'C
Cuts at: 2359 2607 2359
Size: 248 4677
BbsI GAAGACnn'nnnn_
Cuts at: 1217 1567 1217
Size: 350 4575
BciVI GTATCCnnnnn_n'
Cuts at: 386 2823 3584 4925 386
Size: 2437 761 1341 386
Fragments arranged by size:
2437 1341 761 386
BclI T'GATC_A
Cuts at: 2445 2445
Size: 4925
BglII A'GATC_T
Cuts at: 669 1728 2440 4477 669
Size: 1059 712 2037 1117
Fragments arranged by size:
2037 1117 1059 712
BlpI GC'TnA_GC
Cuts at: 2037 2037
Size: 4925
Bme1580I G_kGCm'C
Cuts at: 2550 2643 3699 2550
Size: 93 1056 3776
Fragments arranged by size:
3776 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2137 2218 2553 4697 2137
Size: 81 335 2144 2365
Fragments arranged by size:
2365 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 275 3292 275
Size: 3017 1908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 725 1040 1226 1346 534
Size: 191 315 186 120 4113
Fragments arranged by size:
4113 315 191 186 120
Bpu10I CC'TnA_GC
Cuts at: 926 926
Size: 4925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 2980 3472 3770 2101
Size: 879 492 298 3256
Fragments arranged by size:
3256 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4629 4629
Size: 4925
BsaAI yAC'GTr
Cuts at: 212 2278 212
Size: 2066 2859
BsaBI GATnn'nnATC
Cuts at: 6 2439 4476 6
Size: 2433 2037 455
Fragments arranged by size:
2433 2037 455
BsaHI Gr'CG_yC
Cuts at: 1570 2356 2604 3306 4802 1570
Size: 786 248 702 1496 1693
Fragments arranged by size:
1693 1496 786 702 248
BsaWI w'CCGG_w
Cuts at: 1448 2114 2635 3313 3587 3734 4145 1448
Size: 666 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 666 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 698 728 698
Size: 30 4895
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 356 1411 356
Size: 1055 3870
BseYI C'CCAG_C
Cuts at: 229 2350 3108 3685 4181 229
Size: 2121 758 577 496 973
Fragments arranged by size:
2121 973 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2467 2513 3721 104
Size: 2363 46 1208 1308
Fragments arranged by size:
2363 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1854 1939 2717 2907 3699 1854
Size: 85 778 190 792 3080
Fragments arranged by size:
3080 792 778 190 85
Bsp1286I G_dGCh'C
Cuts at: 1854 1939 2550 2643 2717 2907 2969 3699 1854
Size: 85 611 93 74 190 62 730 3080
Fragments arranged by size:
3080 730 611 190 93 85 74 62
BspEI T'CCGG_A
Cuts at: 1448 4145 1448
Size: 2697 2228
BspHI T'CATG_A
Cuts at: 1232 4101 1232
Size: 2869 2056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1975 2189 2491 2872 1975
Size: 214 302 381 4028
Fragments arranged by size:
4028 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3217 3271 4755 4920 50
Size: 3167 54 1484 165 55
Fragments arranged by size:
3167 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 608 2837 608
Size: 2229 2696
BsrFI r'CCGG_y
Cuts at: 2923 3104 3313 2923
Size: 181 209 4535
Fragments arranged by size:
4535 209 181
BssSI C'ACGA_G
Cuts at: 260 3196 3554 260
Size: 2936 358 1631
Fragments arranged by size:
2936 1631 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2302 2302
Size: 4925
BstBI TT'CG_AA
Cuts at: 3286 3286
Size: 4925
BstYI r'GATC_y
Cuts at: 1 669 1728 1950 2417 2440 2775 3021 4033
Size: 668 1059 222 467 23 335 246 1012
Cuts at: 4033 4119 4131 4477 1
Size: 86 12 346 449
Fragments arranged by size:
1059 1012 668 467 449 346 335 246
222 86 23 12
BtgI C'CryG_G
Cuts at: 510 1030 1262 510
Size: 520 232 4173
Fragments arranged by size:
4173 520 232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2875 3056 3219 2875
Size: 181 163 4581
Fragments arranged by size:
4581 181 163
BtsI GCAGTG_nn'
Cuts at: 2168 2168
Size: 4925
DraI TTT'AAA
Cuts at: 885 1924 885
Size: 1039 3886
DrdI GACnn_nn'nnGTC
Cuts at: 657 1569 2631 3489 657
Size: 912 1062 858 2093
Fragments arranged by size:
2093 1062 912 858
EaeI y'GGCC_r
Cuts at: 643 2510 2684 3075 3102 4447 643
Size: 1867 174 391 27 1345 1121
Fragments arranged by size:
1867 1345 1121 391 174 27
EagI C'GGCC_G
Cuts at: 2510 2510
Size: 4925
EarI CTCTTCn'nnn_
Cuts at: 1388 2948 3158 1388
Size: 1560 210 3155
Fragments arranged by size:
3155 1560 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3133 3443 3589 4735 3133
Size: 310 146 1146 3323
Fragments arranged by size:
3323 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1937 1937
Size: 4925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 534 725 1040 1226 1346 1679 2750 3182
Size: 97 191 315 186 120 333 1071 432
Cuts at: 3182 3929 4197 437
Size: 747 268 1165
Fragments arranged by size:
1165 1071 747 432 333 315 268 191
186 120 97
EcoO109I rG'GnC_Cy
Cuts at: 700 2064 700
Size: 1364 3561
EcoRI G'AATT_C
Cuts at: 1929 1929
Size: 4925
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4925
FspI TGC'GCA
Cuts at: 2706 2706
Size: 4925
HaeII r_GCGC'y
Cuts at: 158 2359 2607 3629 4608 158
Size: 2201 248 1022 979 475
Fragments arranged by size:
2201 1022 979 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1227 1259 2767 2797 2799 2829 1227
Size: 32 1508 30 2 30 3323
Fragments arranged by size:
3323 1508 32 30 30 2
HincII GTy'rAC
Cuts at: 414 1964 414
Size: 1550 3375
HpaI GTT'AAC
Cuts at: 414 414
Size: 4925
KasI G'GCGC_C
Cuts at: 2355 2603 2355
Size: 248 4677
KpnI G_GTAC'C
Cuts at: 1945 1945
Size: 4925
MfeI C'AATT_G
Cuts at: 747 1747 747
Size: 1000 3925
MluI A'CGCG_T
Cuts at: 4358 4358
Size: 4925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3596 3780 3596
Size: 184 4741
MscI TGG'CCA
Cuts at: 645 2686 645
Size: 2041 2884
MslI CAynn'nnrTG
Cuts at: 283 2914 3041 283
Size: 2631 127 2167
Fragments arranged by size:
2631 2167 127
MspA1I CmG'CkG
Cuts at: 1140 2036 2350 2710 3723 3968 1140
Size: 896 314 360 1013 245 2097
Fragments arranged by size:
2097 1013 896 360 314 245
NaeI GCC'GGC
Cuts at: 3106 3106
Size: 4925
NarI GG'CG_CC
Cuts at: 2356 2604 2356
Size: 248 4677
NcoI C'CATG_G
Cuts at: 1030 1030
Size: 4925
NgoMIV G'CCGG_C
Cuts at: 3104 3104
Size: 4925
NspI r_CATG'y
Cuts at: 864 874 1045 1778 1978 2478 3385 864
Size: 10 171 733 200 500 907 2404
Fragments arranged by size:
2404 907 733 500 200 171 10
PciI A'CATG_T
Cuts at: 1774 3381 1774
Size: 1607 3318
PfoI T'CCnGG_A
Cuts at: 1499 1499
Size: 4925
PmeI GTTT'AAAC
Cuts at: 1924 1924
Size: 4925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4893
PpuMI rG'GwC_Cy
Cuts at: 700 700
Size: 4925
PstI C_TGCA'G
Cuts at: 388 1972 2657 388
Size: 1584 685 2656
Fragments arranged by size:
2656 1584 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4925
PvuII CAG'CTG
Cuts at: 1140 2350 2710 1140
Size: 1210 360 3355
Fragments arranged by size:
3355 1210 360
RsrII CG'GwC_CG
Cuts at: 3120 3120
Size: 4925
SacI G_AGCT'C
Cuts at: 1939 1939
Size: 4925
SalI G'TCGA_C
Cuts at: 1962 1962
Size: 4925
SapI GCTCTTCn'nnn_
Cuts at: 2948 3158 2948
Size: 210 4715
SbfI CC_TGCA'GG
Cuts at: 1972 1972
Size: 4925
ScaI AGT'ACT
Cuts at: 1069 4442 1069
Size: 3373 1552
SfcI C'TryA_G
Cuts at: 33 384 1968 2653 3646 3837 33
Size: 351 1584 685 993 191 1121
Fragments arranged by size:
1584 1121 993 685 351 191
SfoI GGC'GCC
Cuts at: 2357 2605 2357
Size: 248 4677
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
SmaI CCC'GGG
Cuts at: 1947 1947
Size: 4925
SmlI C'TyrA_G
Cuts at: 2080 2995 3487 3749 2080
Size: 915 492 262 3256
Fragments arranged by size:
3256 915 492 262
SnaBI TAC'GTA
Cuts at: 212 212
Size: 4925
SpeI A'CTAG_T
Cuts at: 67 1341 67
Size: 1274 3651
SphI G_CATG'C
Cuts at: 864 1978 2478 864
Size: 1114 500 3311
Fragments arranged by size:
3311 1114 500
SspI AAT'ATT
Cuts at: 1620 1620
Size: 4925
StyI C'CwwG_G
Cuts at: 1030 1057 2059 2413 1030
Size: 27 1002 354 3542
Fragments arranged by size:
3542 1002 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2508 2641 3100 3309 2508
Size: 133 459 209 4124
Fragments arranged by size:
4124 459 209 133
TatI w'GTAC_w
Cuts at: 1067 1885 4440 1067
Size: 818 2555 1552
Fragments arranged by size:
2555 1552 818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 698 730 4442 4474 698
Size: 32 3712 32 1149
Fragments arranged by size:
3712 1149 32 32
Tth111I GACn'n_nGTC
Cuts at: 295 2722 295
Size: 2427 2498
XbaI T'CTAG_A
Cuts at: 1956 1956
Size: 4925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1201 2420 1201
Size: 1219 3706
XmaI C'CCGG_G
Cuts at: 1945 1945
Size: 4925
ZraI GAC'GTC
Cuts at: 1571 1571
Size: 4925
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI
XmnI