Restriction map for pF1KB6172
 (Linear) (Six-base) MAPSORT of: KIBB6172.seq  Check: 6323  from: 1  to: 1812



KIBB6172.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AatII G_ACGT'C

Cuts at:      0    1465    1812 
   Size:       1465     347
 
AccI GT'mk_AC

Cuts at:      0     100    1401    1812 
   Size:        100    1301     411

  Fragments arranged by size: 

                1301     411     100
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1571    1812 
   Size:        329    1242     241

  Fragments arranged by size: 

                1242     329     241
 
AflIII A'CryG_T

Cuts at:      0      53    1666    1812 
   Size:         53    1613     146

  Fragments arranged by size: 

                1613     146      53
 
AleI CACnn'nnGTG

Cuts at:      0     175    1812 
   Size:        175    1637
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     779    1812 
   Size:        779    1033
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1522    1812 
   Size:       1522     290
 
ApoI r'AATT_y

Cuts at:      0      33     297     354    1812 
   Size:         33     264      57    1458

  Fragments arranged by size: 

                1458     264      57      33
 
AseI AT'TA_AT

Cuts at:      0    1145    1336    1421    1812 
   Size:       1145     191      85     391

  Fragments arranged by size: 

                1145     391     191      85
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1109    1459    1812 
   Size:       1109     350     353

  Fragments arranged by size: 

                1109     353     350
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     278    1812 
   Size:        278    1534
 
BglII A'GATC_T

Cuts at:      0     561    1620    1812 
   Size:        561    1059     192

  Fragments arranged by size: 

                1059     561     192
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     167    1812 
   Size:        167    1645
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     426     617     932    1118    1238    1812 
   Size:        426     191     315     186     120     574

  Fragments arranged by size: 

                 574     426     315     191     186     120
 
Bpu10I CC'TnA_GC

Cuts at:      0     818    1812 
   Size:        818     994
 
BsaAI yAC'GTr

Cuts at:      0     104    1812 
   Size:        104    1708
 
BsaHI Gr'CG_yC

Cuts at:      0    1462    1812 
   Size:       1462     350
 
BsaWI w'CCGG_w

Cuts at:      0    1340    1812 
   Size:       1340     472
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     590     620    1812 
   Size:        590      30    1192

  Fragments arranged by size: 

                1192     590      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     248    1303    1812 
   Size:        248    1055     509

  Fragments arranged by size: 

                1055     509     248
 
BseYI C'CCAG_C

Cuts at:      0     121    1812 
   Size:        121    1691
 
BsiHKAI G_wGCw'C

Cuts at:      0    1746    1812 
   Size:       1746      66
 
Bsp1286I G_dGCh'C

Cuts at:      0    1746    1812 
   Size:       1746      66
 
BspEI T'CCGG_A

Cuts at:      0    1340    1812 
   Size:       1340     472
 
BspHI T'CATG_A

Cuts at:      0    1124    1812 
   Size:       1124     688
 
BsrDI GCAATG_nn'

Cuts at:      0     500    1812 
   Size:        500    1312
 
BssSI C'ACGA_G

Cuts at:      0     152    1812 
   Size:        152    1660
 
BstYI r'GATC_y

Cuts at:      0     561    1620    1812 
   Size:        561    1059     192

  Fragments arranged by size: 

                1059     561     192
 
BtgI C'CryG_G

Cuts at:      0     402     922    1154    1812 
   Size:        402     520     232     658

  Fragments arranged by size: 

                 658     520     402     232
 
DraI TTT'AAA

Cuts at:      0     777    1812 
   Size:        777    1035
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     549    1461    1812 
   Size:        549     912     351

  Fragments arranged by size: 

                 912     549     351
 
EaeI y'GGCC_r

Cuts at:      0     535    1812 
   Size:        535    1277
 
EarI CTCTTCn'nnn_

Cuts at:      0    1280    1812 
   Size:       1280     532
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     426     617     932    1118    1238    1571    1812 
   Size:        329      97     191     315     186     120     333     241

  Fragments arranged by size: 

                 333     329     315     241     191     186     120      97
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     592    1812 
   Size:        592    1220
 
HaeII r_GCGC'y

Cuts at:      0      50    1812 
   Size:         50    1762
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1119    1151    1812 
   Size:       1119      32     661

  Fragments arranged by size: 

                1119     661      32
 
HincII GTy'rAC

Cuts at:      0     306    1812 
   Size:        306    1506
 
HpaI GTT'AAC

Cuts at:      0     306    1812 
   Size:        306    1506
 
MfeI C'AATT_G

Cuts at:      0     639    1639    1812 
   Size:        639    1000     173

  Fragments arranged by size: 

                1000     639     173
 
MscI TGG'CCA

Cuts at:      0     537    1812 
   Size:        537    1275
 
MslI CAynn'nnrTG

Cuts at:      0     175    1812 
   Size:        175    1637
 
MspA1I CmG'CkG

Cuts at:      0    1032    1812 
   Size:       1032     780
 
NcoI C'CATG_G

Cuts at:      0     922    1812 
   Size:        922     890
 
NspI r_CATG'y

Cuts at:      0     756     766     937    1670    1812 
   Size:        756      10     171     733     142

  Fragments arranged by size: 

                 756     733     171     142      10
 
PciI A'CATG_T

Cuts at:      0    1666    1812 
   Size:       1666     146
 
PfoI T'CCnGG_A

Cuts at:      0    1391    1812 
   Size:       1391     421
 
PpuMI rG'GwC_Cy

Cuts at:      0     592    1812 
   Size:        592    1220
 
PstI C_TGCA'G

Cuts at:      0     280    1812 
   Size:        280    1532
 
PvuII CAG'CTG

Cuts at:      0    1032    1812 
   Size:       1032     780
 
ScaI AGT'ACT

Cuts at:      0     961    1812 
   Size:        961     851
 
SfcI C'TryA_G

Cuts at:      0     276    1812 
   Size:        276    1536
 
SnaBI TAC'GTA

Cuts at:      0     104    1812 
   Size:        104    1708
 
SpeI A'CTAG_T

Cuts at:      0    1233    1812 
   Size:       1233     579
 
SphI G_CATG'C

Cuts at:      0     756    1812 
   Size:        756    1056
 
SspI AAT'ATT

Cuts at:      0    1512    1812 
   Size:       1512     300
 
StyI C'CwwG_G

Cuts at:      0     922     949    1812 
   Size:        922      27     863

  Fragments arranged by size: 

                 922     863      27
 
TatI w'GTAC_w

Cuts at:      0     959    1777    1812 
   Size:        959     818      35

  Fragments arranged by size: 

                 959     818      35
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     590     622    1812 
   Size:        590      32    1190

  Fragments arranged by size: 

                1190     590      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     187    1812 
   Size:        187    1625
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1093    1812 
   Size:       1093     719
 
ZraI GAC'GTC

Cuts at:      0    1463    1812 
   Size:       1463     349
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI
     AseI     BbsI    BciVI    BglII     BplI     BpmI   Bpu10I    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI
    BspHI    BsrDI    BssSI    BstYI     BtgI     DraI     DrdI     EaeI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     HpaI     MfeI
     MscI     MslI   MspA1I     NcoI     NspI     PciI     PfoI    PpuMI
     PstI    PvuII     ScaI     SfcI    SnaBI     SpeI     SphI     SspI
     StyI     TatI     TstI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaBI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     KasI     KpnI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SrfI     StuI
     SwaI    TaqII     XbaI     XhoI     XmaI     XmnI
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