Restriction map for
pF1KB9766
(Circular) (Six-base) MAPSORT of: pF1KB9766.seq Check: 8930 from: 1 to: 5912
pF1KB9766 5912 bp
With 182 enzymes: SgfI *
August 26, 2010 17:36 ..
AccI GT'mk_AC
Cuts at: 1474 1718 2950 1474
Size: 244 1232 4436
Fragments arranged by size:
4436 1232 244
Acc65I G'GTAC_C
Cuts at: 2928 2928
Size: 5912
AclI AA'CG_TT
Cuts at: 5752 5752
Size: 5912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 266 398 838 2858 3737 4169 4916 5184
Size: 127 132 440 2020 879 432 747 268
Cuts at: 5184 139
Size: 867
Fragments arranged by size:
2020 879 867 747 440 432 268 132
127
AflIII A'CryG_T
Cuts at: 1223 2415 3264 4368 5345 1223
Size: 1192 849 1104 977 1790
Fragments arranged by size:
1790 1192 1104 977 849
AgeI A'CCGG_T
Cuts at: 4300 4300
Size: 5912
AhdI GACnn_n'nnGTC
Cuts at: 339 339
Size: 5912
AleI CACnn'nnGTG
Cuts at: 189 1003 2420 189
Size: 814 1417 3681
Fragments arranged by size:
3681 1417 814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 773 773
Size: 5912
AlwNI CAG_nnn'CTG
Cuts at: 345 693 1077 2433 3217 4784 345
Size: 348 384 1356 784 1567 1473
Fragments arranged by size:
1567 1473 1356 784 384 348
ApaI G_GGCC'C
Cuts at: 732 1528 732
Size: 796 5116
ApaLI G'TGCA_C
Cuts at: 4682 4682
Size: 5912
ApoI r'AATT_y
Cuts at: 2020 2818 2916 5265 5521 2020
Size: 798 98 2349 256 2411
Fragments arranged by size:
2411 2349 798 256 98
AscI GG'CGCG_CC
Cuts at: 1508 1508
Size: 5912
AseI AT'TA_AT
Cuts at: 20 20
Size: 5912
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5912
AvaI C'yCGr_G
Cuts at: 134 169 216 478 665 830 979 1408 1467
Size: 35 47 262 187 165 149 429 59
Cuts at: 1467 2801 2932 134
Size: 1334 131 3114
Fragments arranged by size:
3114 1334 429 262 187 165 149 131
59 47 35
BamHI G'GATC_C
Cuts at: 178 2937 178
Size: 2759 3153
BanI G'GyrC_C
Cuts at: 465 527 975 1330 1551 2928 3342 3590 3625
Size: 62 448 355 221 1377 414 248 35
Cuts at: 3625 465
Size: 2752
Fragments arranged by size:
2752 1377 448 414 355 248 221 62
35
BanII G_rGCy'C
Cuts at: 141 715 732 837 1214 1528 1667 1771 2663
Size: 574 17 105 377 314 139 104 892
Cuts at: 2663 2707 2926 3956 141
Size: 44 219 1030 2097
Fragments arranged by size:
2097 1030 892 574 377 314 219 139
105 104 44 17
BbeI G_GCGC'C
Cuts at: 469 979 1555 3346 3594 469
Size: 510 576 1791 248 2787
Fragments arranged by size:
2787 1791 576 510 248
BbsI GAAGACnn'nnnn_
Cuts at: 1088 1866 1088
Size: 778 5134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 784 818 784
Size: 34 5878
BciVI GTATCCnnnnn_n'
Cuts at: 3810 4571 5912 3810
Size: 761 1341 3810
Fragments arranged by size:
3810 1341 761
BclI T'GATC_A
Cuts at: 2168 3432 2168
Size: 1264 4648
BglI GCCn_nnn'nGGC
Cuts at: 536 1054 1624 1627 536
Size: 518 570 3 4821
Fragments arranged by size:
4821 570 518 3
BglII A'GATC_T
Cuts at: 3427 5464 3427
Size: 2037 3875
BlpI GC'TnA_GC
Cuts at: 3024 3024
Size: 5912
Bme1580I G_kGCm'C
Cuts at: 732 1528 2808 3537 3630 4686 732
Size: 796 1280 729 93 1056 1958
Fragments arranged by size:
1958 1280 1056 796 729 93
BmgBI CAC'GTC
Cuts at: 354 2418 354
Size: 2064 3848
BmrI ACTGGGnnnn_n'
Cuts at: 727 2619 3124 3205 3540 5684 727
Size: 1892 505 81 335 2144 955
Fragments arranged by size:
2144 1892 955 505 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 801 4279 801
Size: 3478 2434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 875 1501 2223 416
Size: 459 626 722 4105
Fragments arranged by size:
4105 722 626 459
Bpu10I CC'TnA_GC
Cuts at: 2113 2113
Size: 5912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 3088 3967 4459 4757 1681
Size: 1407 879 492 298 2836
Fragments arranged by size:
2836 1407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5616 5616
Size: 5912
BsaAI yAC'GTr
Cuts at: 147 1023 3265 147
Size: 876 2242 2794
Fragments arranged by size:
2794 2242 876
BsaBI GATnn'nnATC
Cuts at: 6 2403 3426 5463 6
Size: 2397 1023 2037 455
Fragments arranged by size:
2397 2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 466 899 976 1205 1552 3343 3591 4293 5789
Size: 433 77 229 347 1791 248 702 1496
Cuts at: 5789 466
Size: 589
Fragments arranged by size:
1791 1496 702 589 433 347 248 229
77
BsaWI w'CCGG_w
Cuts at: 3101 3622 4300 4574 4721 5132 3101
Size: 521 678 274 147 411 3881
Fragments arranged by size:
3881 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2626 2656 2626
Size: 30 5882
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 787 790 811 872 875 1288 1291 1455 2647
Size: 3 21 61 3 413 3 164 1192
Cuts at: 2647 787
Size: 4052
Fragments arranged by size:
4052 1192 413 164 61 21 3 3
3
BseYI C'CCAG_C
Cuts at: 198 493 578 1517 1965 3337 4095 4672 5168
Size: 295 85 939 448 1372 758 577 496
Cuts at: 5168 198
Size: 942
Fragments arranged by size:
1372 942 939 758 577 496 448 295
85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 2834 1631
Size: 1203 4709
BsiEI CG_ry'CG
Cuts at: 104 1166 1430 3454 3500 4708 104
Size: 1062 264 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 1062 264 46
BsiHKAI G_wGCw'C
Cuts at: 1697 2058 2663 2707 2926 3704 3894 4686 1697
Size: 361 605 44 219 778 190 792 2923
Fragments arranged by size:
2923 792 778 605 361 219 190 44
BsmBI CGTCTCn'nnnn_
Cuts at: 749 749
Size: 5912
Bsp1286I G_dGCh'C
Cuts at: 141 715 732 837 1214 1528 1667 1697 1771
Size: 574 17 105 377 314 139 30 74
Cuts at: 1771 2058 2663 2707 2808 2926 3537 3630 3704
Size: 287 605 44 101 118 611 93 74
Cuts at: 3704 3894 3956 4686 141
Size: 190 62 730 1367
Fragments arranged by size:
1367 730 611 605 574 377 314 287
190 139 118 105 101 93 74 74
62 44 30 17
BspEI T'CCGG_A
Cuts at: 5132 5132
Size: 5912
BspHI T'CATG_A
Cuts at: 599 5088 599
Size: 4489 1423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 196 1704 2962 3176 3478 3859 196
Size: 1508 1258 214 302 381 2249
Fragments arranged by size:
2249 1508 1258 381 302 214
BsrBI CCG'CTC
Cuts at: 50 597 1258 1565 4204 4258 5742 5907 50
Size: 547 661 307 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 661 547 307 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 2311 3824 1895
Size: 416 1513 3983
Fragments arranged by size:
3983 1513 416
BsrFI r'CCGG_y
Cuts at: 561 605 1327 3910 4091 4300 561
Size: 44 722 2583 181 209 2173
Fragments arranged by size:
2583 2173 722 209 181 44
BsrGI T'GTAC_A
Cuts at: 1493 1493
Size: 5912
BssHII G'CGCG_C
Cuts at: 1300 1508 1300
Size: 208 5704
BssSI C'ACGA_G
Cuts at: 4183 4541 4183
Size: 358 5554
BstAPI GCAn_nnn'nTGC
Cuts at: 693 2062 3289 693
Size: 1369 1227 3316
Fragments arranged by size:
3316 1369 1227
BstBI TT'CG_AA
Cuts at: 2325 4273 2325
Size: 1948 3964
BstEII G'GTnAC_C
Cuts at: 513 513
Size: 5912
BstXI CCAn_nnnn'nTGG
Cuts at: 1008 2868 1008
Size: 1860 4052
BstYI r'GATC_y
Cuts at: 1 178 555 2404 2516 2937 3404 3427 3762
Size: 177 377 1849 112 421 467 23 335
Cuts at: 3762 4008 5020 5106 5118 5464 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1849 1012 467 449 421 377 346 335
246 177 112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 471 1741 471
Size: 1270 4642
BtgI C'CryG_G
Cuts at: 866 1001 1132 1579 866
Size: 135 131 447 5199
Fragments arranged by size:
5199 447 135 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3862 4043 4206 3862
Size: 181 163 5568
Fragments arranged by size:
5568 181 163
BtsI GCAGTG_nn'
Cuts at: 184 3155 184
Size: 2971 2941
DraI TTT'AAA
Cuts at: 2025 2911 2025
Size: 886 5026
DraIII CAC_nnn'GTG
Cuts at: 1147 2812 1147
Size: 1665 4247
DrdI GACnn_nn'nnGTC
Cuts at: 3618 4476 3618
Size: 858 5054
EaeI y'GGCC_r
Cuts at: 254 995 1325 1427 3497 3671 4062 4089 5434
Size: 741 330 102 2070 174 391 27 1345
Cuts at: 5434 254
Size: 732
Fragments arranged by size:
2070 1345 741 732 391 330 174 102
27
EagI C'GGCC_G
Cuts at: 1427 3497 1427
Size: 2070 3842
EarI CTCTTCn'nnn_
Cuts at: 289 2549 3935 4145 289
Size: 2260 1386 210 2056
Fragments arranged by size:
2260 2056 1386 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1593 4120 4430 4576 5722 1593
Size: 2527 310 146 1146 1783
Fragments arranged by size:
2527 1783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2661 2705 2924 2661
Size: 44 219 5649
Fragments arranged by size:
5649 219 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 266 398 416 838 875 1501 2223 2858
Size: 127 132 18 422 37 626 722 635
Cuts at: 2858 3737 4169 4916 5184 139
Size: 879 432 747 268 867
Fragments arranged by size:
879 867 747 722 635 626 432 422
268 132 127 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1711 2603 1711
Size: 892 5020
EcoO109I rG'GnC_Cy
Cuts at: 130 309 475 669 728 729 1524 1704 3051
Size: 179 166 194 59 1 795 180 1347
Cuts at: 3051 130
Size: 2991
Fragments arranged by size:
2991 1347 795 194 180 179 166 59
1
EcoRI G'AATT_C
Cuts at: 2916 2916
Size: 5912
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1893 3931 1893
Size: 2038 3874
FspI TGC'GCA
Cuts at: 3693 3693
Size: 5912
HaeII r_GCGC'y
Cuts at: 469 940 979 987 1121 1555 3346 3594 4616
Size: 471 39 8 134 434 1791 248 1022
Cuts at: 4616 5595 469
Size: 979 786
Fragments arranged by size:
1791 1022 979 786 471 434 248 134
39 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 325 357 1813 1845 2626 2648 2658
Size: 32 130 32 1456 32 781 22 10
Cuts at: 2658 2680 3754 3784 3786 3816 163
Size: 22 1074 30 2 30 2259
Fragments arranged by size:
2259 1456 1074 781 130 32 32 32
30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 1475 2951 1475
Size: 1476 4436
HindIII A'AGCT_T
Cuts at: 2536 2536
Size: 5912
KasI G'GCGC_C
Cuts at: 465 975 1551 3342 3590 465
Size: 510 576 1791 248 2787
Fragments arranged by size:
2787 1791 576 510 248
KpnI G_GTAC'C
Cuts at: 2932 2932
Size: 5912
MfeI C'AATT_G
Cuts at: 2672 2672
Size: 5912
MluI A'CGCG_T
Cuts at: 1223 5345 1223
Size: 4122 1790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 156 329 341 603 4583 4767 156
Size: 173 12 262 3980 184 1301
Fragments arranged by size:
3980 1301 262 184 173 12
MscI TGG'CCA
Cuts at: 997 3673 997
Size: 2676 3236
MslI CAynn'nnrTG
Cuts at: 189 1003 1006 1021 1844 2339 2420 3901 4028
Size: 814 3 15 823 495 81 1481 127
Cuts at: 4028 189
Size: 2073
Fragments arranged by size:
2073 1481 823 814 495 127 81 15
3
MspA1I CmG'CkG
Cuts at: 431 582 646 690 757 868 993 1074 1134
Size: 151 64 44 67 111 125 81 60
Cuts at: 1134 1380 1581 2469 3023 3337 3697 4710 4955
Size: 246 201 888 554 314 360 1013 245
Cuts at: 4955 431
Size: 1388
Fragments arranged by size:
1388 1013 888 554 360 314 246 245
201 151 125 111 81 67 64 60
44
NaeI GCC'GGC
Cuts at: 4093 4093
Size: 5912
NarI GG'CG_CC
Cuts at: 466 976 1552 3343 3591 466
Size: 510 576 1791 248 2787
Fragments arranged by size:
2787 1791 576 510 248
NgoMIV G'CCGG_C
Cuts at: 4091 4091
Size: 5912
NspI r_CATG'y
Cuts at: 1973 2965 3465 4372 1973
Size: 992 500 907 3513
Fragments arranged by size:
3513 992 907 500
PasI CC'CwG_GG
Cuts at: 941 941
Size: 5912
PciI A'CATG_T
Cuts at: 4368 4368
Size: 5912
PflMI CCAn_nnn'nTGG
Cuts at: 524 524
Size: 5912
PfoI T'CCnGG_A
Cuts at: 634 1349 634
Size: 715 5197
PmeI GTTT'AAAC
Cuts at: 2911 2911
Size: 5912
PmlI CAC'GTG
Cuts at: 147 1023 147
Size: 876 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5051 5083 5051
Size: 32 5880
PpuMI rG'GwC_Cy
Cuts at: 130 309 475 130
Size: 179 166 5567
Fragments arranged by size:
5567 179 166
PspOMI G'GGCC_C
Cuts at: 728 1524 728
Size: 796 5116
PstI C_TGCA'G
Cuts at: 2959 3644 2959
Size: 685 5227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5912
PvuII CAG'CTG
Cuts at: 646 690 1074 3337 3697 646
Size: 44 384 2263 360 2861
Fragments arranged by size:
2861 2263 384 360 44
RsrII CG'GwC_CG
Cuts at: 608 4107 608
Size: 3499 2413
SacI G_AGCT'C
Cuts at: 2663 2707 2926 2663
Size: 44 219 5649
Fragments arranged by size:
5649 219 44
SacII CC_GC'GG
Cuts at: 869 1135 1582 869
Size: 266 447 5199
Fragments arranged by size:
5199 447 266
SalI G'TCGA_C
Cuts at: 1473 2949 1473
Size: 1476 4436
SanDI GG'GwC_CC
Cuts at: 130 130
Size: 5912
SapI GCTCTTCn'nnn_
Cuts at: 3935 4145 3935
Size: 210 5702
SbfI CC_TGCA'GG
Cuts at: 2959 2959
Size: 5912
ScaI AGT'ACT
Cuts at: 5429 5429
Size: 5912
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 5912
SfcI C'TryA_G
Cuts at: 33 1169 1236 2955 3640 4633 4824 33
Size: 1136 67 1719 685 993 191 1121
Fragments arranged by size:
1719 1136 1121 993 685 191 67
SfoI GGC'GCC
Cuts at: 467 977 1553 3344 3592 467
Size: 510 576 1791 248 2787
Fragments arranged by size:
2787 1791 576 510 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5912
SmaI CCC'GGG
Cuts at: 136 218 480 667 981 1410 2803 2934 136
Size: 82 262 187 314 429 1393 131 3114
Fragments arranged by size:
3114 1393 429 314 262 187 131 82
SmlI C'TyrA_G
Cuts at: 1696 3067 3982 4474 4736 1696
Size: 1371 915 492 262 2872
Fragments arranged by size:
2872 1371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5912
SphI G_CATG'C
Cuts at: 2965 3465 2965
Size: 500 5412
SrfI GCCC'GGGC
Cuts at: 981 981
Size: 5912
StuI AGG'CCT
Cuts at: 1294 1294
Size: 5912
StyI C'CwwG_G
Cuts at: 914 1214 2007 3046 3400 914
Size: 300 793 1039 354 3426
Fragments arranged by size:
3426 1039 793 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 829 3495 3628 4087 4296 592
Size: 237 2666 133 459 209 2208
Fragments arranged by size:
2666 2208 459 237 209 133
TatI w'GTAC_w
Cuts at: 1493 2774 5427 1493
Size: 1281 2653 1978
Fragments arranged by size:
2653 1978 1281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5429 5461 5429
Size: 32 5880
Tth111I GACn'n_nGTC
Cuts at: 445 3709 445
Size: 3264 2648
XbaI T'CTAG_A
Cuts at: 2943 2943
Size: 5912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 948 1005 3407 948
Size: 57 2402 3453
Fragments arranged by size:
3453 2402 57
XmaI C'CCGG_G
Cuts at: 134 216 478 665 979 1408 2801 2932 134
Size: 82 262 187 314 429 1393 131 3114
Fragments arranged by size:
3114 1393 429 314 262 187 131 82
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BsmI BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SfiI SgrAI
SnaBI SspI SwaI XhoI XmnI ZraI