(Circular) (Six-base) MAPSORT of: pF1KE2256.seq Check: 8397 from: 1 to: 4370 pF1KE2256 4370 bp With 182 enzymes: SgfI * July 20, 2018 17:51 .. AatII G_ACGT'C Cuts at: 455 455 Size: 4370 AccI GT'mk_AC Cuts at: 383 689 1408 383 Size: 306 719 3345 Fragments arranged by size: 3345 719 306 Acc65I G'GTAC_C Cuts at: 1386 1386 Size: 4370 AclI AA'CG_TT Cuts at: 4210 4210 Size: 4370 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 2627 3374 3642 2195 Size: 432 747 268 2923 Fragments arranged by size: 2923 747 432 268 AfeI AGC'GCT Cuts at: 769 769 Size: 4370 AflIII A'CryG_T Cuts at: 1722 2826 3803 1722 Size: 1104 977 2289 Fragments arranged by size: 2289 1104 977 AgeI A'CCGG_T Cuts at: 2758 2758 Size: 4370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1366 1366 Size: 4370 AlwNI CAG_nnn'CTG Cuts at: 567 1218 1675 3242 567 Size: 651 457 1567 1695 Fragments arranged by size: 1695 1567 651 457 ApaLI G'TGCA_C Cuts at: 462 1201 3140 462 Size: 739 1939 1692 Fragments arranged by size: 1939 1692 739 ApoI r'AATT_y Cuts at: 1374 3723 3979 1374 Size: 2349 256 1765 Fragments arranged by size: 2349 1765 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4370 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4370 AvaI C'yCGr_G Cuts at: 917 1390 917 Size: 473 3897 BamHI G'GATC_C Cuts at: 1395 1395 Size: 4370 BanI G'GyrC_C Cuts at: 577 609 1386 1800 2048 2083 577 Size: 32 777 414 248 35 2864 Fragments arranged by size: 2864 777 414 248 35 32 BanII G_rGCy'C Cuts at: 421 1384 2414 421 Size: 963 1030 2377 Fragments arranged by size: 2377 1030 963 BbeI G_GCGC'C Cuts at: 581 613 1804 2052 581 Size: 32 1191 248 2899 Fragments arranged by size: 2899 1191 248 32 BbsI GAAGACnn'nnnn_ Cuts at: 1305 1329 1305 Size: 24 4346 BciVI GTATCCnnnnn_n' Cuts at: 2268 3029 4370 2268 Size: 761 1341 2268 Fragments arranged by size: 2268 1341 761 BclI T'GATC_A Cuts at: 1890 1890 Size: 4370 BglI GCCn_nnn'nGGC Cuts at: 169 442 1245 169 Size: 273 803 3294 Fragments arranged by size: 3294 803 273 BglII A'GATC_T Cuts at: 1885 3922 1885 Size: 2037 2333 BlpI GC'TnA_GC Cuts at: 415 1482 415 Size: 1067 3303 Bme1580I G_kGCm'C Cuts at: 466 1205 1241 1995 2088 3144 466 Size: 739 36 754 93 1056 1692 Fragments arranged by size: 1692 1056 754 739 93 36 BmgBI CAC'GTC Cuts at: 636 784 636 Size: 148 4222 BmrI ACTGGGnnnn_n' Cuts at: 753 1582 1663 1998 4142 753 Size: 829 81 335 2144 981 Fragments arranged by size: 2144 981 829 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 2737 Size: 4370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 688 688 Size: 4370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1546 2425 2917 3215 1546 Size: 879 492 298 2701 Fragments arranged by size: 2701 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4074 4074 Size: 4370 BsaAI yAC'GTr Cuts at: 584 684 1723 584 Size: 100 1039 3231 Fragments arranged by size: 3231 1039 100 BsaBI GATnn'nnATC Cuts at: 6 1884 3921 6 Size: 1878 2037 455 Fragments arranged by size: 2037 1878 455 BsaHI Gr'CG_yC Cuts at: 307 434 452 578 610 1049 1186 1801 2049 Size: 127 18 126 32 439 137 615 248 Cuts at: 2049 2751 4247 307 Size: 702 1496 430 Fragments arranged by size: 1496 702 615 439 430 248 137 127 126 32 18 BsaWI w'CCGG_w Cuts at: 1559 2080 2758 3032 3179 3590 1559 Size: 521 678 274 147 411 2339 Fragments arranged by size: 2339 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1092 1122 1092 Size: 30 4340 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 340 343 340 Size: 3 4367 BseYI C'CCAG_C Cuts at: 148 199 770 820 955 1269 1795 2553 3130 Size: 51 571 50 135 314 526 758 577 Cuts at: 3130 3626 148 Size: 496 892 Fragments arranged by size: 892 758 577 571 526 496 314 135 51 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 781 781 Size: 4370 BsiEI CG_ry'CG Cuts at: 104 164 341 645 804 1125 1912 1958 3166 Size: 60 177 304 159 321 787 46 1208 Cuts at: 3166 104 Size: 1308 Fragments arranged by size: 1308 1208 787 321 304 177 159 60 46 BsiHKAI G_wGCw'C Cuts at: 254 466 706 1041 1205 1384 2162 2352 3144 Size: 212 240 335 164 179 778 190 792 Cuts at: 3144 254 Size: 1480 Fragments arranged by size: 1480 792 778 335 240 212 190 179 164 Bsp1286I G_dGCh'C Cuts at: 254 421 466 706 1041 1205 1241 1384 1995 Size: 167 45 240 335 164 36 143 611 Cuts at: 1995 2088 2162 2352 2414 3144 254 Size: 93 74 190 62 730 1480 Fragments arranged by size: 1480 730 611 335 240 190 167 164 143 93 74 62 45 36 BspEI T'CCGG_A Cuts at: 3590 3590 Size: 4370 BspHI T'CATG_A Cuts at: 3546 3546 Size: 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1420 1634 1936 2317 1420 Size: 214 302 381 3473 Fragments arranged by size: 3473 381 302 214 BsrBI CCG'CTC Cuts at: 50 375 726 2662 2716 4200 4365 50 Size: 325 351 1936 54 1484 165 55 Fragments arranged by size: 1936 1484 351 325 165 55 54 BsrDI GCAATG_nn' Cuts at: 2282 2282 Size: 4370 BsrFI r'CCGG_y Cuts at: 159 539 557 1120 1152 2368 2549 2758 159 Size: 380 18 563 32 1216 181 209 1771 Fragments arranged by size: 1771 1216 563 380 209 181 32 18 BssHII G'CGCG_C Cuts at: 294 806 294 Size: 512 3858 BssSI C'ACGA_G Cuts at: 268 2641 2999 268 Size: 2373 358 1639 Fragments arranged by size: 2373 1639 358 BstAPI GCAn_nnn'nTGC Cuts at: 567 1747 567 Size: 1180 3190 BstBI TT'CG_AA Cuts at: 2731 2731 Size: 4370 BstXI CCAn_nnnn'nTGG Cuts at: 498 498 Size: 4370 BstYI r'GATC_y Cuts at: 1 1395 1862 1885 2220 2466 3478 3564 3576 Size: 1394 467 23 335 246 1012 86 12 Cuts at: 3576 3922 1 Size: 346 449 Fragments arranged by size: 1394 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 125 164 446 515 596 756 1243 107 Size: 18 39 282 69 81 160 487 3234 Fragments arranged by size: 3234 487 282 160 81 69 39 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2320 2501 2664 2320 Size: 181 163 4026 Fragments arranged by size: 4026 181 163 BtsI GCAGTG_nn' Cuts at: 1613 1613 Size: 4370 DraI TTT'AAA Cuts at: 1369 1369 Size: 4370 DrdI GACnn_nn'nnGTC Cuts at: 685 2076 2934 685 Size: 1391 858 2121 Fragments arranged by size: 2121 1391 858 EaeI y'GGCC_r Cuts at: 161 284 338 443 512 518 642 801 1122 Size: 123 54 105 69 6 124 159 321 Cuts at: 1122 1955 2129 2520 2547 3892 161 Size: 833 174 391 27 1345 639 Fragments arranged by size: 1345 833 639 391 321 174 159 124 123 105 69 54 27 6 EagI C'GGCC_G Cuts at: 161 338 642 801 1122 1955 161 Size: 177 304 159 321 833 2576 Fragments arranged by size: 2576 833 321 304 177 159 EarI CTCTTCn'nnn_ Cuts at: 382 2393 2603 382 Size: 2011 210 2149 Fragments arranged by size: 2149 2011 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2578 2888 3034 4180 2578 Size: 310 146 1146 2768 Fragments arranged by size: 2768 1146 310 146 EcoICRI GAG'CTC Cuts at: 1382 1382 Size: 4370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 688 2195 2627 3374 3642 688 Size: 1507 432 747 268 1416 Fragments arranged by size: 1507 1416 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 868 964 868 Size: 96 4274 EcoO109I rG'GnC_Cy Cuts at: 873 922 969 1146 1166 1347 1509 873 Size: 49 47 177 20 181 162 3734 Fragments arranged by size: 3734 181 177 162 49 47 20 EcoRI G'AATT_C Cuts at: 1374 1374 Size: 4370 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2389 2389 Size: 4370 FseI GG_CCGG'CC Cuts at: 163 163 Size: 4370 FspI TGC'GCA Cuts at: 2151 2151 Size: 4370 HaeII r_GCGC'y Cuts at: 220 581 613 771 1219 1259 1289 1804 2052 Size: 361 32 158 448 40 30 515 248 Cuts at: 2052 3074 4053 220 Size: 1022 979 537 Fragments arranged by size: 1022 979 537 515 448 361 248 158 40 32 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2212 2242 2244 2274 2212 Size: 30 2 30 4308 Fragments arranged by size: 4308 30 30 2 HincII GTy'rAC Cuts at: 1281 1409 1281 Size: 128 4242 KasI G'GCGC_C Cuts at: 577 609 1800 2048 577 Size: 32 1191 248 2899 Fragments arranged by size: 2899 1191 248 32 KpnI G_GTAC'C Cuts at: 1390 1390 Size: 4370 MluI A'CGCG_T Cuts at: 3803 3803 Size: 4370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1055 3041 3225 1055 Size: 1986 184 2200 Fragments arranged by size: 2200 1986 184 MscI TGG'CCA Cuts at: 286 445 2131 286 Size: 159 1686 2525 Fragments arranged by size: 2525 1686 159 MslI CAynn'nnrTG Cuts at: 445 496 2359 2486 445 Size: 51 1863 127 2329 Fragments arranged by size: 2329 1863 127 51 MspA1I CmG'CkG Cuts at: 127 166 528 564 598 758 824 1245 1481 Size: 39 362 36 34 160 66 421 236 Cuts at: 1481 1795 2155 3168 3413 127 Size: 314 360 1013 245 1084 Fragments arranged by size: 1084 1013 421 362 360 314 245 236 160 66 39 36 34 NaeI GCC'GGC Cuts at: 161 559 2551 161 Size: 398 1992 1980 Fragments arranged by size: 1992 1980 398 NarI GG'CG_CC Cuts at: 578 610 1801 2049 578 Size: 32 1191 248 2899 Fragments arranged by size: 2899 1191 248 32 NcoI C'CATG_G Cuts at: 107 446 107 Size: 339 4031 NgoMIV G'CCGG_C Cuts at: 159 557 2549 159 Size: 398 1992 1980 Fragments arranged by size: 1992 1980 398 NotI GC'GGCC_GC Cuts at: 338 642 338 Size: 304 4066 NspI r_CATG'y Cuts at: 1423 1923 2830 1423 Size: 500 907 2963 Fragments arranged by size: 2963 907 500 PciI A'CATG_T Cuts at: 2826 2826 Size: 4370 PmeI GTTT'AAAC Cuts at: 1369 1369 Size: 4370 PmlI CAC'GTG Cuts at: 584 584 Size: 4370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 3509 Size: 32 4338 PpuMI rG'GwC_Cy Cuts at: 873 922 969 1146 873 Size: 49 47 177 4097 Fragments arranged by size: 4097 177 49 47 PstI C_TGCA'G Cuts at: 305 767 1417 2102 305 Size: 462 650 685 2573 Fragments arranged by size: 2573 685 650 462 PvuI CG_AT'CG Cuts at: 104 104 Size: 4370 PvuII CAG'CTG Cuts at: 564 1795 2155 564 Size: 1231 360 2779 Fragments arranged by size: 2779 1231 360 RsrII CG'GwC_CG Cuts at: 2565 2565 Size: 4370 SacI G_AGCT'C Cuts at: 1384 1384 Size: 4370 SacII CC_GC'GG Cuts at: 128 167 599 759 1246 128 Size: 39 432 160 487 3252 Fragments arranged by size: 3252 487 432 160 39 SalI G'TCGA_C Cuts at: 1407 1407 Size: 4370 SanDI GG'GwC_CC Cuts at: 922 922 Size: 4370 SapI GCTCTTCn'nnn_ Cuts at: 382 2393 2603 382 Size: 2011 210 2149 Fragments arranged by size: 2149 2011 210 SbfI CC_TGCA'GG Cuts at: 1417 1417 Size: 4370 ScaI AGT'ACT Cuts at: 3887 3887 Size: 4370 SfcI C'TryA_G Cuts at: 33 301 763 1413 2098 3091 3282 33 Size: 268 462 650 685 993 191 1121 Fragments arranged by size: 1121 993 685 650 462 268 191 SfoI GGC'GCC Cuts at: 579 611 1802 2050 579 Size: 32 1191 248 2899 Fragments arranged by size: 2899 1191 248 32 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4370 SmaI CCC'GGG Cuts at: 919 1392 919 Size: 473 3897 SmlI C'TyrA_G Cuts at: 1525 2440 2932 3194 1525 Size: 915 492 262 2701 Fragments arranged by size: 2701 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4370 SphI G_CATG'C Cuts at: 1423 1923 1423 Size: 500 3870 StyI C'CwwG_G Cuts at: 107 446 1504 1858 107 Size: 339 1058 354 2619 Fragments arranged by size: 2619 1058 354 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1953 2086 2545 2754 1953 Size: 133 459 209 3569 Fragments arranged by size: 3569 459 209 133 TatI w'GTAC_w Cuts at: 3885 3885 Size: 4370 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1092 1124 3887 3919 1092 Size: 32 2763 32 1543 Fragments arranged by size: 2763 1543 32 32 Tth111I GACn'n_nGTC Cuts at: 1051 2167 1051 Size: 1116 3254 XbaI T'CTAG_A Cuts at: 1401 1401 Size: 4370 XcmI CCAnnnn_n'nnnnTGG Cuts at: 973 1865 973 Size: 892 3478 XmaI C'CCGG_G Cuts at: 917 1390 917 Size: 473 3897 ZraI GAC'GTC Cuts at: 453 453 Size: 4370 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI