(Circular) (Six-base) MAPSORT of: pF1KE2339.seq Check: 9993 from: 1 to: 3983 pF1KE2339 3983 bp With 182 enzymes: SgfI * May 6, 2016 19:10 .. AccI GT'mk_AC Cuts at: 1021 1021 Size: 3983 Acc65I G'GTAC_C Cuts at: 999 999 Size: 3983 AclI AA'CG_TT Cuts at: 3823 3823 Size: 3983 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 346 1808 2240 2987 3255 346 Size: 1462 432 747 268 1074 Fragments arranged by size: 1462 1074 747 432 268 AflIII A'CryG_T Cuts at: 1335 2439 3416 1335 Size: 1104 977 1902 Fragments arranged by size: 1902 1104 977 AgeI A'CCGG_T Cuts at: 688 2371 688 Size: 1683 2300 AlwNI CAG_nnn'CTG Cuts at: 324 423 1288 2855 324 Size: 99 865 1567 1452 Fragments arranged by size: 1567 1452 865 99 ApaLI G'TGCA_C Cuts at: 2753 2753 Size: 3983 ApoI r'AATT_y Cuts at: 987 3336 3592 987 Size: 2349 256 1378 Fragments arranged by size: 2349 1378 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3983 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3983 AvaI C'yCGr_G Cuts at: 192 1003 192 Size: 811 3172 BamHI G'GATC_C Cuts at: 1008 1008 Size: 3983 BanI G'GyrC_C Cuts at: 631 999 1413 1661 1696 631 Size: 368 414 248 35 2918 Fragments arranged by size: 2918 414 368 248 35 BanII G_rGCy'C Cuts at: 314 997 2027 314 Size: 683 1030 2270 Fragments arranged by size: 2270 1030 683 BbeI G_GCGC'C Cuts at: 1417 1665 1417 Size: 248 3735 BbsI GAAGACnn'nnnn_ Cuts at: 334 334 Size: 3983 BbvCI CC'TCA_GC Cuts at: 151 151 Size: 3983 BciVI GTATCCnnnnn_n' Cuts at: 1881 2642 3983 1881 Size: 761 1341 1881 Fragments arranged by size: 1881 1341 761 BclI T'GATC_A Cuts at: 1503 1503 Size: 3983 BglI GCCn_nnn'nGGC Cuts at: 355 355 Size: 3983 BglII A'GATC_T Cuts at: 599 1498 3535 599 Size: 899 2037 1047 Fragments arranged by size: 2037 1047 899 BlpI GC'TnA_GC Cuts at: 1095 1095 Size: 3983 Bme1580I G_kGCm'C Cuts at: 1608 1701 2757 1608 Size: 93 1056 2834 Fragments arranged by size: 2834 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 474 576 1195 1276 1611 3755 474 Size: 102 619 81 335 2144 702 Fragments arranged by size: 2144 702 619 335 102 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 2350 Size: 3983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 416 429 416 Size: 13 3970 Bpu10I CC'TnA_GC Cuts at: 151 151 Size: 3983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1159 2038 2530 2828 1159 Size: 879 492 298 2314 Fragments arranged by size: 2314 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3687 3687 Size: 3983 BsaAI yAC'GTr Cuts at: 1336 1336 Size: 3983 BsaBI GATnn'nnATC Cuts at: 6 1497 3534 6 Size: 1491 2037 455 Fragments arranged by size: 2037 1491 455 BsaHI Gr'CG_yC Cuts at: 180 713 1414 1662 2364 3860 180 Size: 533 701 248 702 1496 303 Fragments arranged by size: 1496 702 701 533 303 248 BsaWI w'CCGG_w Cuts at: 688 695 920 1172 1693 2371 2645 2792 3203 Size: 7 225 252 521 678 274 147 411 Cuts at: 3203 688 Size: 1468 Fragments arranged by size: 1468 678 521 411 274 252 225 147 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 427 457 427 Size: 30 3953 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 352 352 Size: 3983 BseYI C'CCAG_C Cuts at: 1408 2166 2743 3239 1408 Size: 758 577 496 2152 Fragments arranged by size: 2152 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 803 803 Size: 3983 BsiEI CG_ry'CG Cuts at: 104 350 1525 1571 2779 104 Size: 246 1175 46 1208 1308 Fragments arranged by size: 1308 1208 1175 246 46 BsiHKAI G_wGCw'C Cuts at: 314 997 1775 1965 2757 314 Size: 683 778 190 792 1540 Fragments arranged by size: 1540 792 778 683 190 BsmI GAATG_Cn' Cuts at: 846 846 Size: 3983 Bsp1286I G_dGCh'C Cuts at: 314 997 1608 1701 1775 1965 2027 2757 314 Size: 683 611 93 74 190 62 730 1540 Fragments arranged by size: 1540 730 683 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3203 3203 Size: 3983 BspHI T'CATG_A Cuts at: 3159 3159 Size: 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 715 1033 1247 1549 1930 715 Size: 318 214 302 381 2768 Fragments arranged by size: 2768 381 318 302 214 BsrBI CCG'CTC Cuts at: 50 2275 2329 3813 3978 50 Size: 2225 54 1484 165 55 Fragments arranged by size: 2225 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1895 1895 Size: 3983 BsrFI r'CCGG_y Cuts at: 370 568 688 1981 2162 2371 370 Size: 198 120 1293 181 209 1982 Fragments arranged by size: 1982 1293 209 198 181 120 BssSI C'ACGA_G Cuts at: 2254 2612 2254 Size: 358 3625 BstAPI GCAn_nnn'nTGC Cuts at: 1360 1360 Size: 3983 BstBI TT'CG_AA Cuts at: 2344 2344 Size: 3983 BstEII G'GTnAC_C Cuts at: 691 691 Size: 3983 BstYI r'GATC_y Cuts at: 1 599 698 1008 1475 1498 1833 2079 3091 Size: 598 99 310 467 23 335 246 1012 Cuts at: 3091 3177 3189 3535 1 Size: 86 12 346 449 Fragments arranged by size: 1012 598 467 449 346 335 310 246 99 86 23 12 BtgI C'CryG_G Cuts at: 107 141 245 350 107 Size: 34 104 105 3740 Fragments arranged by size: 3740 105 104 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 201 308 483 685 835 1933 2114 2277 201 Size: 107 175 202 150 1098 181 163 1907 Fragments arranged by size: 1907 1098 202 181 175 163 150 107 BtsI GCAGTG_nn' Cuts at: 1226 1226 Size: 3983 DraI TTT'AAA Cuts at: 982 982 Size: 3983 DrdI GACnn_nn'nnGTC Cuts at: 1689 2547 1689 Size: 858 3125 EaeI y'GGCC_r Cuts at: 110 347 824 1568 1742 2133 2160 3505 110 Size: 237 477 744 174 391 27 1345 588 Fragments arranged by size: 1345 744 588 477 391 237 174 27 EagI C'GGCC_G Cuts at: 347 1568 347 Size: 1221 2762 EarI CTCTTCn'nnn_ Cuts at: 2006 2216 2006 Size: 210 3773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2191 2501 2647 3793 2191 Size: 310 146 1146 2381 Fragments arranged by size: 2381 1146 310 146 EcoICRI GAG'CTC Cuts at: 312 995 312 Size: 683 3300 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 346 416 429 1808 2240 2987 3255 346 Size: 70 13 1379 432 747 268 1074 Fragments arranged by size: 1379 1074 747 432 268 70 13 EcoNI CCTnn'n_nnAGG Cuts at: 478 478 Size: 3983 EcoO109I rG'GnC_Cy Cuts at: 155 276 321 342 1122 155 Size: 121 45 21 780 3016 Fragments arranged by size: 3016 780 121 45 21 EcoRI G'AATT_C Cuts at: 987 987 Size: 3983 EcoRV GAT'ATC Cuts at: 405 558 405 Size: 153 3830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 745 909 981 2002 745 Size: 164 72 1021 2726 Fragments arranged by size: 2726 1021 164 72 FspI TGC'GCA Cuts at: 1764 1764 Size: 3983 HaeII r_GCGC'y Cuts at: 1417 1665 2687 3666 1417 Size: 248 1022 979 1734 Fragments arranged by size: 1734 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 389 421 427 459 685 717 1825 1855 1857 Size: 32 6 32 226 32 1108 30 2 Cuts at: 1857 1887 389 Size: 30 2485 Fragments arranged by size: 2485 1108 226 32 32 32 30 30 6 2 HincII GTy'rAC Cuts at: 1022 1022 Size: 3983 KasI G'GCGC_C Cuts at: 1413 1661 1413 Size: 248 3735 KpnI G_GTAC'C Cuts at: 1003 1003 Size: 3983 MluI A'CGCG_T Cuts at: 3416 3416 Size: 3983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 282 860 2654 2838 282 Size: 578 1794 184 1427 Fragments arranged by size: 1794 1427 578 184 MscI TGG'CCA Cuts at: 826 1744 826 Size: 918 3065 MslI CAynn'nnrTG Cuts at: 1972 2099 1972 Size: 127 3856 MspA1I CmG'CkG Cuts at: 143 150 394 1094 1408 1768 2781 3026 143 Size: 7 244 700 314 360 1013 245 1100 Fragments arranged by size: 1100 1013 700 360 314 245 244 7 NaeI GCC'GGC Cuts at: 2164 2164 Size: 3983 NarI GG'CG_CC Cuts at: 1414 1662 1414 Size: 248 3735 NcoI C'CATG_G Cuts at: 107 107 Size: 3983 NgoMIV G'CCGG_C Cuts at: 2162 2162 Size: 3983 NspI r_CATG'y Cuts at: 1036 1536 2443 1036 Size: 500 907 2576 Fragments arranged by size: 2576 907 500 PciI A'CATG_T Cuts at: 2439 2439 Size: 3983 PfoI T'CCnGG_A Cuts at: 431 431 Size: 3983 PmeI GTTT'AAAC Cuts at: 982 982 Size: 3983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3122 3154 3122 Size: 32 3951 PpuMI rG'GwC_Cy Cuts at: 342 342 Size: 3983 PstI C_TGCA'G Cuts at: 1030 1715 1030 Size: 685 3298 PvuI CG_AT'CG Cuts at: 104 104 Size: 3983 PvuII CAG'CTG Cuts at: 394 1408 1768 394 Size: 1014 360 2609 Fragments arranged by size: 2609 1014 360 RsrII CG'GwC_CG Cuts at: 2178 2178 Size: 3983 SacI G_AGCT'C Cuts at: 314 997 314 Size: 683 3300 SacII CC_GC'GG Cuts at: 144 144 Size: 3983 SalI G'TCGA_C Cuts at: 1020 1020 Size: 3983 SapI GCTCTTCn'nnn_ Cuts at: 2006 2216 2006 Size: 210 3773 SbfI CC_TGCA'GG Cuts at: 1030 1030 Size: 3983 ScaI AGT'ACT Cuts at: 3500 3500 Size: 3983 SexAI A'CCwGG_T Cuts at: 974 974 Size: 3983 SfcI C'TryA_G Cuts at: 33 1026 1711 2704 2895 33 Size: 993 685 993 191 1121 Fragments arranged by size: 1121 993 993 685 191 SfiI GGCCn_nnn'nGGCC Cuts at: 355 355 Size: 3983 SfoI GGC'GCC Cuts at: 1415 1663 1415 Size: 248 3735 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3983 SgrAI Cr'CCGG_yG Cuts at: 370 688 370 Size: 318 3665 SmaI CCC'GGG Cuts at: 1005 1005 Size: 3983 SmlI C'TyrA_G Cuts at: 1138 2053 2545 2807 1138 Size: 915 492 262 2314 Fragments arranged by size: 2314 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3983 SphI G_CATG'C Cuts at: 1036 1536 1036 Size: 500 3483 StuI AGG'CCT Cuts at: 385 385 Size: 3983 StyI C'CwwG_G Cuts at: 107 272 1117 1471 107 Size: 165 845 354 2619 Fragments arranged by size: 2619 845 354 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 493 649 1566 1699 2158 2367 493 Size: 156 917 133 459 209 2109 Fragments arranged by size: 2109 917 459 209 156 133 TatI w'GTAC_w Cuts at: 209 3498 209 Size: 3289 694 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3500 3532 3500 Size: 32 3951 Tth111I GACn'n_nGTC Cuts at: 1780 1780 Size: 3983 XbaI T'CTAG_A Cuts at: 1014 1014 Size: 3983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1478 1478 Size: 3983 XmaI C'CCGG_G Cuts at: 1003 1003 Size: 3983 XmnI GAAnn'nnTTC Cuts at: 331 331 Size: 3983 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SnaBI SrfI SspI SwaI XhoI ZraI