(Circular) (Six-base) MAPSORT of: pF1KE2487.seq Check: 4996 from: 1 to: 5165 pF1KE2487 5165 bp With 182 enzymes: SgfI * October 10, 2013 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1908 1908 Size: 5165 AccI GT'mk_AC Cuts at: 1622 2203 1622 Size: 581 4584 Acc65I G'GTAC_C Cuts at: 131 2181 131 Size: 2050 3115 AclI AA'CG_TT Cuts at: 5005 5005 Size: 5165 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 390 784 2990 3422 4169 4437 390 Size: 394 2206 432 747 268 1118 Fragments arranged by size: 2206 1118 747 432 394 268 AflIII A'CryG_T Cuts at: 2517 3621 4598 2517 Size: 1104 977 3084 Fragments arranged by size: 3084 1104 977 AgeI A'CCGG_T Cuts at: 3553 3553 Size: 5165 AhdI GACnn_n'nnGTC Cuts at: 1044 1044 Size: 5165 AlwNI CAG_nnn'CTG Cuts at: 2470 4037 2470 Size: 1567 3598 ApaLI G'TGCA_C Cuts at: 3935 3935 Size: 5165 ApoI r'AATT_y Cuts at: 547 2169 4518 4774 547 Size: 1622 2349 256 938 Fragments arranged by size: 2349 1622 938 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5165 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5165 AvaI C'yCGr_G Cuts at: 959 1463 2185 959 Size: 504 722 3939 Fragments arranged by size: 3939 722 504 BamHI G'GATC_C Cuts at: 311 2190 311 Size: 1879 3286 BanI G'GyrC_C Cuts at: 131 540 2181 2595 2843 2878 131 Size: 409 1641 414 248 35 2418 Fragments arranged by size: 2418 1641 414 409 248 35 BanII G_rGCy'C Cuts at: 355 748 2179 3209 355 Size: 393 1431 1030 2311 Fragments arranged by size: 2311 1431 1030 393 BbeI G_GCGC'C Cuts at: 2599 2847 2599 Size: 248 4917 BbsI GAAGACnn'nnnn_ Cuts at: 359 482 714 1335 1947 359 Size: 123 232 621 612 3577 Fragments arranged by size: 3577 621 612 232 123 BbvCI CC'TCA_GC Cuts at: 881 881 Size: 5165 BciVI GTATCCnnnnn_n' Cuts at: 3063 3824 5165 3063 Size: 761 1341 3063 Fragments arranged by size: 3063 1341 761 BclI T'GATC_A Cuts at: 612 1521 1646 2685 612 Size: 909 125 1039 3092 Fragments arranged by size: 3092 1039 909 125 BglI GCCn_nnn'nGGC Cuts at: 1194 1194 Size: 5165 BglII A'GATC_T Cuts at: 2680 4717 2680 Size: 2037 3128 BlpI GC'TnA_GC Cuts at: 532 1833 2277 532 Size: 1301 444 3420 Fragments arranged by size: 3420 1301 444 Bme1580I G_kGCm'C Cuts at: 348 2790 2883 3939 348 Size: 2442 93 1056 1574 Fragments arranged by size: 2442 1574 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1536 2377 2458 2793 4937 1536 Size: 841 81 335 2144 1764 Fragments arranged by size: 2144 1764 841 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2124 3532 2124 Size: 1408 3757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1156 1302 1156 Size: 146 5019 Bpu10I CC'TnA_GC Cuts at: 747 881 2109 747 Size: 134 1228 3803 Fragments arranged by size: 3803 1228 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 661 1694 1984 2005 2341 3220 3712 4010 661 Size: 1033 290 21 336 879 492 298 1816 Fragments arranged by size: 1816 1033 879 492 336 298 290 21 BsaI GGTCTCn'nnnn_ Cuts at: 1109 4869 1109 Size: 3760 1405 BsaAI yAC'GTr Cuts at: 2518 2518 Size: 5165 BsaBI GATnn'nnATC Cuts at: 6 2679 4716 6 Size: 2673 2037 455 Fragments arranged by size: 2673 2037 455 BsaHI Gr'CG_yC Cuts at: 2596 2844 3546 5042 2596 Size: 248 702 1496 2719 Fragments arranged by size: 2719 1496 702 248 BsaWI w'CCGG_w Cuts at: 569 2354 2875 3553 3827 3974 4385 569 Size: 1785 521 678 274 147 411 1349 Fragments arranged by size: 1785 1349 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1963 1993 1963 Size: 30 5135 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 109 203 577 634 1246 109 Size: 94 374 57 612 4028 Fragments arranged by size: 4028 612 374 94 57 BseYI C'CCAG_C Cuts at: 470 829 2590 3348 3925 4421 470 Size: 359 1761 758 577 496 1214 Fragments arranged by size: 1761 1214 758 577 496 359 BsiEI CG_ry'CG Cuts at: 104 2707 2753 3961 104 Size: 2603 46 1208 1308 Fragments arranged by size: 2603 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 2179 2957 3147 3939 2179 Size: 778 190 792 3405 Fragments arranged by size: 3405 792 778 190 Bsp1286I G_dGCh'C Cuts at: 348 355 748 2179 2790 2883 2957 3147 3209 Size: 7 393 1431 611 93 74 190 62 Cuts at: 3209 3939 348 Size: 730 1574 Fragments arranged by size: 1574 1431 730 611 393 190 93 74 62 7 BspEI T'CCGG_A Cuts at: 569 4385 569 Size: 3816 1349 BspHI T'CATG_A Cuts at: 1562 1667 4341 1562 Size: 105 2674 2386 Fragments arranged by size: 2674 2386 105 BspMI ACCTGCnnnn'nnnn_ Cuts at: 714 979 1556 1667 1908 2215 2429 2731 3112 Size: 265 577 111 241 307 214 302 381 Cuts at: 3112 714 Size: 2767 Fragments arranged by size: 2767 577 381 307 302 265 241 214 111 BsrBI CCG'CTC Cuts at: 50 3457 3511 4995 5160 50 Size: 3407 54 1484 165 55 Fragments arranged by size: 3407 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1455 3077 1455 Size: 1622 3543 BsrFI r'CCGG_y Cuts at: 3163 3344 3553 3163 Size: 181 209 4775 Fragments arranged by size: 4775 209 181 BssSI C'ACGA_G Cuts at: 1678 3436 3794 1678 Size: 1758 358 3049 Fragments arranged by size: 3049 1758 358 BstAPI GCAn_nnn'nTGC Cuts at: 2542 2542 Size: 5165 BstBI TT'CG_AA Cuts at: 3526 3526 Size: 5165 BstEII G'GTnAC_C Cuts at: 1890 1890 Size: 5165 BstXI CCAn_nnnn'nTGG Cuts at: 793 793 Size: 5165 BstYI r'GATC_y Cuts at: 1 311 575 2190 2657 2680 3015 3261 4273 Size: 310 264 1615 467 23 335 246 1012 Cuts at: 4273 4359 4371 4717 1 Size: 86 12 346 449 Fragments arranged by size: 1615 1012 467 449 346 335 310 264 246 86 23 12 BtgI C'CryG_G Cuts at: 932 1572 932 Size: 640 4525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3115 3296 3459 3115 Size: 181 163 4821 Fragments arranged by size: 4821 181 163 BtsI GCAGTG_nn' Cuts at: 1368 2408 1368 Size: 1040 4125 DraI TTT'AAA Cuts at: 2164 2164 Size: 5165 DraIII CAC_nnn'GTG Cuts at: 1427 1427 Size: 5165 DrdI GACnn_nn'nnGTC Cuts at: 2871 3729 2871 Size: 858 4307 EaeI y'GGCC_r Cuts at: 2051 2750 2924 3315 3342 4687 2051 Size: 699 174 391 27 1345 2529 Fragments arranged by size: 2529 1345 699 391 174 27 EagI C'GGCC_G Cuts at: 2750 2750 Size: 5165 EarI CTCTTCn'nnn_ Cuts at: 418 2070 3188 3398 418 Size: 1652 1118 210 2185 Fragments arranged by size: 2185 1652 1118 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 277 1029 3373 3683 3829 4975 277 Size: 752 2344 310 146 1146 467 Fragments arranged by size: 2344 1146 752 467 310 146 EcoICRI GAG'CTC Cuts at: 2177 2177 Size: 5165 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 390 784 1156 1302 2990 3422 4169 4437 390 Size: 394 372 146 1688 432 747 268 1118 Fragments arranged by size: 1688 1118 747 432 394 372 268 146 EcoNI CCTnn'n_nnAGG Cuts at: 655 655 Size: 5165 EcoO109I rG'GnC_Cy Cuts at: 114 183 208 1140 1545 2118 2304 114 Size: 69 25 932 405 573 186 2975 Fragments arranged by size: 2975 932 573 405 186 69 25 EcoRI G'AATT_C Cuts at: 2169 2169 Size: 5165 EcoRV GAT'ATC Cuts at: 697 697 Size: 5165 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1950 3184 1950 Size: 1234 3931 FspI TGC'GCA Cuts at: 2946 2946 Size: 5165 HaeII r_GCGC'y Cuts at: 836 2599 2847 3869 4848 836 Size: 1763 248 1022 979 1153 Fragments arranged by size: 1763 1153 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 855 887 1030 1062 1672 1692 1704 1724 3007 Size: 32 143 32 610 20 12 20 1283 Cuts at: 3007 3037 3039 3069 855 Size: 30 2 30 2951 Fragments arranged by size: 2951 1283 610 143 32 32 30 30 20 20 12 2 HincII GTy'rAC Cuts at: 2204 2204 Size: 5165 KasI G'GCGC_C Cuts at: 2595 2843 2595 Size: 248 4917 KpnI G_GTAC'C Cuts at: 135 2185 135 Size: 2050 3115 MluI A'CGCG_T Cuts at: 4598 4598 Size: 5165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 157 3836 4020 157 Size: 3679 184 1302 Fragments arranged by size: 3679 1302 184 MscI TGG'CCA Cuts at: 2053 2926 2053 Size: 873 4292 MslI CAynn'nnrTG Cuts at: 3154 3281 3154 Size: 127 5038 MspA1I CmG'CkG Cuts at: 630 1270 1280 1686 2276 2590 2950 3963 4208 Size: 640 10 406 590 314 360 1013 245 Cuts at: 4208 630 Size: 1587 Fragments arranged by size: 1587 1013 640 590 406 360 314 245 10 NaeI GCC'GGC Cuts at: 3346 3346 Size: 5165 NarI GG'CG_CC Cuts at: 2596 2844 2596 Size: 248 4917 NcoI C'CATG_G Cuts at: 1572 1572 Size: 5165 NgoMIV G'CCGG_C Cuts at: 3344 3344 Size: 5165 NspI r_CATG'y Cuts at: 1618 2218 2718 3625 1618 Size: 600 500 907 3158 Fragments arranged by size: 3158 907 600 500 PasI CC'CwG_GG Cuts at: 739 1410 739 Size: 671 4494 PciI A'CATG_T Cuts at: 3621 3621 Size: 5165 PflMI CCAn_nnn'nTGG Cuts at: 249 249 Size: 5165 PfoI T'CCnGG_A Cuts at: 320 320 Size: 5165 PmeI GTTT'AAAC Cuts at: 2164 2164 Size: 5165 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 4304 Size: 32 5133 PpuMI rG'GwC_Cy Cuts at: 114 1545 114 Size: 1431 3734 PshAI GACnn'nnGTC Cuts at: 1637 1771 1637 Size: 134 5031 PstI C_TGCA'G Cuts at: 1664 1814 1877 1919 2212 2897 1664 Size: 150 63 42 293 685 3932 Fragments arranged by size: 3932 685 293 150 63 42 PvuI CG_AT'CG Cuts at: 104 104 Size: 5165 PvuII CAG'CTG Cuts at: 630 1270 1686 2590 2950 630 Size: 640 416 904 360 2845 Fragments arranged by size: 2845 904 640 416 360 RsrII CG'GwC_CG Cuts at: 3360 3360 Size: 5165 SacI G_AGCT'C Cuts at: 2179 2179 Size: 5165 SalI G'TCGA_C Cuts at: 2202 2202 Size: 5165 SapI GCTCTTCn'nnn_ Cuts at: 3188 3398 3188 Size: 210 4955 SbfI CC_TGCA'GG Cuts at: 1877 2212 1877 Size: 335 4830 ScaI AGT'ACT Cuts at: 4682 4682 Size: 5165 SfcI C'TryA_G Cuts at: 33 1660 1810 1873 1915 2208 2893 3886 4077 Size: 1627 150 63 42 293 685 993 191 Cuts at: 4077 33 Size: 1121 Fragments arranged by size: 1627 1121 993 685 293 191 150 63 42 SfoI GGC'GCC Cuts at: 2597 2845 2597 Size: 248 4917 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5165 SmaI CCC'GGG Cuts at: 1465 2187 1465 Size: 722 4443 SmlI C'TyrA_G Cuts at: 676 1709 1963 2020 2320 3235 3727 3989 676 Size: 1033 254 57 300 915 492 262 1852 Fragments arranged by size: 1852 1033 915 492 300 262 254 57 SpeI A'CTAG_T Cuts at: 67 67 Size: 5165 SphI G_CATG'C Cuts at: 2218 2718 2218 Size: 500 4665 StuI AGG'CCT Cuts at: 761 761 Size: 5165 StyI C'CwwG_G Cuts at: 248 656 727 1572 2299 2653 248 Size: 408 71 845 727 354 2760 Fragments arranged by size: 2760 845 727 408 354 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1034 2748 2881 3340 3549 1034 Size: 1714 133 459 209 2650 Fragments arranged by size: 2650 1714 459 209 133 TatI w'GTAC_w Cuts at: 4680 4680 Size: 5165 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4682 4714 4682 Size: 32 5133 Tth111I GACn'n_nGTC Cuts at: 1818 2962 1818 Size: 1144 4021 XbaI T'CTAG_A Cuts at: 2196 2196 Size: 5165 XcmI CCAnnnn_n'nnnnTGG Cuts at: 847 2660 847 Size: 1813 3352 XmaI C'CCGG_G Cuts at: 1463 2185 1463 Size: 722 4443 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI