(Linear) (Six-base) MAPSORT of: KIEE2487.seq Check: 6846 from: 1 to: 2052 KIEE2487.orf With 182 enzymes: SgfI * October 10, 2013 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1800 2052 Size: 1800 252 AccI GT'mk_AC Cuts at: 0 1514 2052 Size: 1514 538 Acc65I G'GTAC_C Cuts at: 0 23 2052 Size: 23 2029 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 676 2052 Size: 282 394 1376 Fragments arranged by size: 1376 394 282 AhdI GACnn_n'nnGTC Cuts at: 0 936 2052 Size: 936 1116 ApoI r'AATT_y Cuts at: 0 439 2052 Size: 439 1613 AvaI C'yCGr_G Cuts at: 0 851 1355 2052 Size: 851 504 697 Fragments arranged by size: 851 697 504 BamHI G'GATC_C Cuts at: 0 203 2052 Size: 203 1849 BanI G'GyrC_C Cuts at: 0 23 432 2052 Size: 23 409 1620 Fragments arranged by size: 1620 409 23 BanII G_rGCy'C Cuts at: 0 247 640 2052 Size: 247 393 1412 Fragments arranged by size: 1412 393 247 BbsI GAAGACnn'nnnn_ Cuts at: 0 251 374 606 1227 1839 2052 Size: 251 123 232 621 612 213 Fragments arranged by size: 621 612 251 232 213 123 BbvCI CC'TCA_GC Cuts at: 0 773 2052 Size: 773 1279 BclI T'GATC_A Cuts at: 0 504 1413 1538 2052 Size: 504 909 125 514 Fragments arranged by size: 909 514 504 125 BglI GCCn_nnn'nGGC Cuts at: 0 1086 2052 Size: 1086 966 BlpI GC'TnA_GC Cuts at: 0 424 1725 2052 Size: 424 1301 327 Fragments arranged by size: 1301 424 327 Bme1580I G_kGCm'C Cuts at: 0 240 2052 Size: 240 1812 BmrI ACTGGGnnnn_n' Cuts at: 0 1428 2052 Size: 1428 624 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2016 2052 Size: 2016 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1048 1194 2052 Size: 1048 146 858 Fragments arranged by size: 1048 858 146 Bpu10I CC'TnA_GC Cuts at: 0 639 773 2001 2052 Size: 639 134 1228 51 Fragments arranged by size: 1228 639 134 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1586 1876 1897 2052 Size: 553 1033 290 21 155 Fragments arranged by size: 1033 553 290 155 21 BsaI GGTCTCn'nnnn_ Cuts at: 0 1001 2052 Size: 1001 1051 BsaWI w'CCGG_w Cuts at: 0 461 2052 Size: 461 1591 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1855 1885 2052 Size: 1855 30 167 Fragments arranged by size: 1855 167 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1 95 469 526 1138 2052 Size: 1 94 374 57 612 914 Fragments arranged by size: 914 612 374 94 57 1 BseYI C'CCAG_C Cuts at: 0 362 721 2052 Size: 362 359 1331 Fragments arranged by size: 1331 362 359 Bsp1286I G_dGCh'C Cuts at: 0 240 247 640 2052 Size: 240 7 393 1412 Fragments arranged by size: 1412 393 240 7 BspEI T'CCGG_A Cuts at: 0 461 2052 Size: 461 1591 BspHI T'CATG_A Cuts at: 0 1454 1559 2052 Size: 1454 105 493 Fragments arranged by size: 1454 493 105 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 606 871 1448 1559 1800 2052 Size: 606 265 577 111 241 252 Fragments arranged by size: 606 577 265 252 241 111 BsrDI GCAATG_nn' Cuts at: 0 1347 2052 Size: 1347 705 BssSI C'ACGA_G Cuts at: 0 1570 2052 Size: 1570 482 BstEII G'GTnAC_C Cuts at: 0 1782 2052 Size: 1782 270 BstXI CCAn_nnnn'nTGG Cuts at: 0 685 2052 Size: 685 1367 BstYI r'GATC_y Cuts at: 0 203 467 2052 Size: 203 264 1585 Fragments arranged by size: 1585 264 203 BtgI C'CryG_G Cuts at: 0 824 1464 2052 Size: 824 640 588 Fragments arranged by size: 824 640 588 BtsI GCAGTG_nn' Cuts at: 0 1260 2052 Size: 1260 792 DraIII CAC_nnn'GTG Cuts at: 0 1319 2052 Size: 1319 733 EaeI y'GGCC_r Cuts at: 0 1943 2052 Size: 1943 109 EarI CTCTTCn'nnn_ Cuts at: 0 310 1962 2052 Size: 310 1652 90 Fragments arranged by size: 1652 310 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 169 921 2052 Size: 169 752 1131 Fragments arranged by size: 1131 752 169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 676 1048 1194 2052 Size: 282 394 372 146 858 Fragments arranged by size: 858 394 372 282 146 EcoNI CCTnn'n_nnAGG Cuts at: 0 547 2052 Size: 547 1505 EcoO109I rG'GnC_Cy Cuts at: 0 6 75 100 1032 1437 2010 2052 Size: 6 69 25 932 405 573 42 Fragments arranged by size: 932 573 405 69 42 25 6 EcoRV GAT'ATC Cuts at: 0 589 2052 Size: 589 1463 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1842 2052 Size: 1842 210 HaeII r_GCGC'y Cuts at: 0 728 2052 Size: 728 1324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 747 779 922 954 1564 1584 1596 1616 Size: 747 32 143 32 610 20 12 20 Cuts at: 1616 2052 Size: 436 Fragments arranged by size: 747 610 436 143 32 32 20 20 12 KpnI G_GTAC'C Cuts at: 0 27 2052 Size: 27 2025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 49 2052 Size: 49 2003 MscI TGG'CCA Cuts at: 0 1945 2052 Size: 1945 107 MspA1I CmG'CkG Cuts at: 0 522 1162 1172 1578 2052 Size: 522 640 10 406 474 Fragments arranged by size: 640 522 474 406 10 NcoI C'CATG_G Cuts at: 0 1464 2052 Size: 1464 588 NspI r_CATG'y Cuts at: 0 1510 2052 Size: 1510 542 PasI CC'CwG_GG Cuts at: 0 631 1302 2052 Size: 631 671 750 Fragments arranged by size: 750 671 631 PflMI CCAn_nnn'nTGG Cuts at: 0 141 2052 Size: 141 1911 PfoI T'CCnGG_A Cuts at: 0 212 2052 Size: 212 1840 PpuMI rG'GwC_Cy Cuts at: 0 6 1437 2052 Size: 6 1431 615 Fragments arranged by size: 1431 615 6 PshAI GACnn'nnGTC Cuts at: 0 1529 1663 2052 Size: 1529 134 389 Fragments arranged by size: 1529 389 134 PstI C_TGCA'G Cuts at: 0 1556 1706 1769 1811 2052 Size: 1556 150 63 42 241 Fragments arranged by size: 1556 241 150 63 42 PvuII CAG'CTG Cuts at: 0 522 1162 1578 2052 Size: 522 640 416 474 Fragments arranged by size: 640 522 474 416 SbfI CC_TGCA'GG Cuts at: 0 1769 2052 Size: 1769 283 SfcI C'TryA_G Cuts at: 0 1552 1702 1765 1807 2052 Size: 1552 150 63 42 245 Fragments arranged by size: 1552 245 150 63 42 SmaI CCC'GGG Cuts at: 0 1357 2052 Size: 1357 695 SmlI C'TyrA_G Cuts at: 0 568 1601 1855 1912 2052 Size: 568 1033 254 57 140 Fragments arranged by size: 1033 568 254 140 57 StuI AGG'CCT Cuts at: 0 653 2052 Size: 653 1399 StyI C'CwwG_G Cuts at: 0 140 548 619 1464 2052 Size: 140 408 71 845 588 Fragments arranged by size: 845 588 408 140 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 926 2052 Size: 926 1126 Tth111I GACn'n_nGTC Cuts at: 0 1710 2052 Size: 1710 342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 739 2052 Size: 739 1313 XmaI C'CCGG_G Cuts at: 0 1355 2052 Size: 1355 697 Enzymes that do cut: AarI AccI Acc65I AcuI AhdI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KpnI MmeI MscI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PshAI PstI PvuII SbfI SfcI SmaI SmlI StuI StyI TaqII Tth111I XcmI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmnI ZraI