Restriction map for
pF1KB7582
(Linear) (Six-base) MAPSORT of: KIBB7582.seq Check: 9254 from: 1 to: 861
KIBB7582.orf
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 277 418 861
Size: 146 131 141 443
Fragments arranged by size:
443 146 141 131
AlwNI CAG_nnn'CTG
Cuts at: 0 706 861
Size: 706 155
ApaI G_GGCC'C
Cuts at: 0 234 861
Size: 234 627
ApoI r'AATT_y
Cuts at: 0 547 861
Size: 547 314
BamHI G'GATC_C
Cuts at: 0 408 861
Size: 408 453
BanII G_rGCy'C
Cuts at: 0 234 827 861
Size: 234 593 34
Fragments arranged by size:
593 234 34
Bme1580I G_kGCm'C
Cuts at: 0 234 861
Size: 234 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 758 861
Size: 758 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 861
Size: 788 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 188 218 861
Size: 188 30 643
Fragments arranged by size:
643 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 196 520 708 861
Size: 193 3 324 188 153
Fragments arranged by size:
324 193 188 153 3
BseYI C'CCAG_C
Cuts at: 0 233 257 861
Size: 233 24 604
Fragments arranged by size:
604 233 24
BsiHKAI G_wGCw'C
Cuts at: 0 827 861
Size: 827 34
Bsp1286I G_dGCh'C
Cuts at: 0 234 827 861
Size: 234 593 34
Fragments arranged by size:
593 234 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 322 706 861
Size: 322 384 155
Fragments arranged by size:
384 322 155
BstYI r'GATC_y
Cuts at: 0 395 408 861
Size: 395 13 453
Fragments arranged by size:
453 395 13
EarI CTCTTCn'nnn_
Cuts at: 0 77 529 861
Size: 77 452 332
Fragments arranged by size:
452 332 77
EcoICRI GAG'CTC
Cuts at: 0 825 861
Size: 825 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 277 418 788 861
Size: 146 131 141 370 73
Fragments arranged by size:
370 146 141 131 73
EcoO109I rG'GnC_Cy
Cuts at: 0 16 230 861
Size: 16 214 631
Fragments arranged by size:
631 214 16
EcoRI G'AATT_C
Cuts at: 0 547 861
Size: 547 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 861
Size: 784 32 45
Fragments arranged by size:
784 45 32
MspA1I CmG'CkG
Cuts at: 0 283 861
Size: 283 578
NdeI CA'TA_TG
Cuts at: 0 191 861
Size: 191 670
NspI r_CATG'y
Cuts at: 0 544 700 861
Size: 544 156 161
Fragments arranged by size:
544 161 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 466 861
Size: 466 395
PpuMI rG'GwC_Cy
Cuts at: 0 16 861
Size: 16 845
PspOMI G'GGCC_C
Cuts at: 0 230 861
Size: 230 631
PstI C_TGCA'G
Cuts at: 0 72 114 318 861
Size: 72 42 204 543
Fragments arranged by size:
543 204 72 42
PvuII CAG'CTG
Cuts at: 0 283 861
Size: 283 578
SacI G_AGCT'C
Cuts at: 0 827 861
Size: 827 34
SfcI C'TryA_G
Cuts at: 0 68 110 314 710 861
Size: 68 42 204 396 151
Fragments arranged by size:
396 204 151 68 42
StyI C'CwwG_G
Cuts at: 0 758 788 861
Size: 758 30 73
Fragments arranged by size:
758 73 30
TatI w'GTAC_w
Cuts at: 0 353 861
Size: 353 508
Tth111I GACn'n_nGTC
Cuts at: 0 144 861
Size: 144 717
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BamHI BanII Bme1580I BmrI
BpmI BsaXI BseRI BseYI BsiHKAI Bsp1286I BstAPI BstYI
EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I MspA1I NdeI
NspI PflMI PpuMI PspOMI PstI PvuII SacI SfcI
StyI TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XcmI XhoI XmaI XmnI
ZraI