Restriction map for pF1KB7201
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AocI        ApaI        
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsaBI       BsaWI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrDI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        Eam1105I    
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SalI        SapI        SbfI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        2002  GACGTC               GACGT^C
AccI         1207  GTCTAC               GT^[AC][GT]AC
AlwNI        1426  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AscI         1027  GGCGCGCC             GG^CGCGCC
BalI         1223  TGGCCA               TGG^CCA
BbrPI        1153  CACGTG               CAC^GTG
BlpI         1374  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1374  GCTCAGC              GC^T[ACGT]AGC
BsaI         1165  GAGACC               GGTCTC(1/5)
BseRI        1129  GAGGAG               GAGGAG(10/8)
BsmBI         885  CGTCTC               CGTCTC(1/5)
Bsp1407I      272  TGTACA               T^GTACA
BsrGI         272  TGTACA               T^GTACA
BssSI        1003  CACGAG               CACGAG(-5/-1)
BstEII         10  GGTTACC              G^GT[ACGT]ACC
BstF5I       1527  CATCC                GGATG(2/0)
BstPI          10  GGTTACC              G^GT[ACGT]ACC
BstXI          89  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         230  AGATCC               [GA]^GATC[CT]
CelII        1374  GCTCAGC              GC^T[ACGT]AGC
DdeI         1375  CTCAG                C^T[ACGT]AG
DraIII        435  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I        10  GGTTACC              G^GT[ACGT]ACC
FokI         1527  CATCC                GGATG(9/13)
HinfI        1831  GAGTC                G^A[ACGT]TC
MflI          230  AGATCC               [GA]^GATC[CT]
MluNI        1223  TGGCCA               TGG^CCA
MscI         1223  TGGCCA               TGG^CCA
NotI           76  GCGGCCGC             GC^GGCCGC
PleI         1831  GAGTC                GAGTC(4/5)
PmaCI        1153  CACGTG               CAC^GTG
PmlI         1153  CACGTG               CAC^GTG
SacI         2040  GAGCTC               GAGCT^C
SfiI           78  GGCCGCCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI         272  TGTACA               T^GTACA
TaqI          902  TCGA                 T^CGA
Tsp45I         25  GTGAC                ^GT[GC]AC
TthHB8I       902  TCGA                 T^CGA
XhoII         230  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Aor51HI        59  AGCGCT               AGC^GCT
              911  AGCGCT              
BsaAI        1153  CACGTG               [CT]AC^GT[GA]
             1414  TACGTG              
BsgI          264  GTGCAG               GTGCAG(16/14)
             1017  GTGCAG              
BsmAI        1165  GAGAC                GTCTC(1/5)
              886  GTCTC               
BspMI        1439  ACCTGC               ACCTGC(4/8)
             1715  ACCTGC              
Eco47III       59  AGCGCT               AGC^GCT
              911  AGCGCT              
MaeIII         11  GTTAC                ^GT[ACGT]AC
               25  GTGAC               
MslI          150  CACCTACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1359  CATCGCGGTG          
PflMI        1232  CCATCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1425  CCAGAGCCTGG         
PpuMI        1712  GGGACCT              [GA]G^G[AT]CC[CT]
             1950  GGGACCC             
PstI           55  CTGCAG               CTGCA^G
             1717  CTGCAG              
ScaI         1316  AGTACT               AGT^ACT
             1766  AGTACT              
SmaI          688  CCCGGG               CCC^GGG
             1107  CCCGGG              
SmlI          250  CTCAAG               C^T[CT][GA]AG
             1762  CTCAAG              
SphI          100  GCATGC               GCATG^C
              395  GCATGC              
Van91I       1232  CCATCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1425  CCAGAGCCTGG         
XcmI         1358  CCATCGCGGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1490  CCATGTTCCTGCTGG     
XmaCI         688  CCCGGG               C^CCGGG
             1107  CCCGGG              
XmaI          688  CCCGGG               C^CCGGG
             1107  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        ApaLI       AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssHII      
BssKI       BstAPI      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DpnII       DraII       
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        StyI        TseI        TspRI       
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