Restriction map for pF1KB7201
 (Linear) (Six-base) MAPSORT of: KIBB7201.seq  Check: 3631  from: 1  to: 2082



KIBB7201.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AatII G_ACGT'C

Cuts at:      0    2006    2082 
   Size:       2006      76
 
AccI GT'mk_AC

Cuts at:      0    1208    2082 
   Size:       1208     874
 
AfeI AGC'GCT

Cuts at:      0      61     913    2082 
   Size:         61     852    1169

  Fragments arranged by size: 

                1169     852      61
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1431    2082 
   Size:       1431     651
 
ApaLI G'TGCA_C

Cuts at:      0     205    1185    1848    2082 
   Size:        205     980     663     234

  Fragments arranged by size: 

                 980     663     234     205
 
AscI GG'CGCG_CC

Cuts at:      0    1028    2082 
   Size:       1028    1054
 
AvaI C'yCGr_G

Cuts at:      0     415     688    1107    1797    2082 
   Size:        415     273     419     690     285

  Fragments arranged by size: 

                 690     419     415     285     273
 
BanI G'GyrC_C

Cuts at:      0     615     692    1458    1486    1876    1900    2082 
   Size:        615      77     766      28     390      24     182

  Fragments arranged by size: 

                 766     615     390     182      77      28      24
 
BanII G_rGCy'C

Cuts at:      0     689    1990    2044    2082 
   Size:        689    1301      54      38

  Fragments arranged by size: 

                1301     689      54      38
 
BbeI G_GCGC'C

Cuts at:      0     619    1462    1880    1904    2082 
   Size:        619     843     418      24     178

  Fragments arranged by size: 

                 843     619     418     178      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     825    1188    1746    2082 
   Size:        825     363     558     336

  Fragments arranged by size: 

                 825     558     363     336
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46      85     374    2082 
   Size:         46      39     289    1708

  Fragments arranged by size: 

                1708     289      46      39
 
BlpI GC'TnA_GC

Cuts at:      0    1375    2082 
   Size:       1375     707
 
Bme1580I G_kGCm'C

Cuts at:      0     209    1189    1346    1628    1852    2035    2082 
   Size:        209     980     157     282     224     183      47

  Fragments arranged by size: 

                 980     282     224     209     183     157      47
 
BmgBI CAC'GTC

Cuts at:      0    2021    2082 
   Size:       2021      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    1597    1849    2082 
   Size:        220    1377     252     233

  Fragments arranged by size: 

                1377     252     233     220
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235    1747    2082 
   Size:        235    1512     335

  Fragments arranged by size: 

                1512     335     235
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1159    2082 
   Size:       1159     923
 
BsaAI yAC'GTr

Cuts at:      0    1155    1416    2082 
   Size:       1155     261     666

  Fragments arranged by size: 

                1155     666     261
 
BsaHI Gr'CG_yC

Cuts at:      0     616    1459    1877    1901    2003    2082 
   Size:        616     843     418      24     102      79

  Fragments arranged by size: 

                 843     616     418     102      79      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     220     250    2082 
   Size:        220      30    1832

  Fragments arranged by size: 

                1832     220      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1144    2082 
   Size:       1144     938
 
BseYI C'CCAG_C

Cuts at:      0     481     621    1048    1134    1345    1679    1862    2082 
   Size:        481     140     427      86     211     334     183     220

  Fragments arranged by size: 

                 481     427     334     220     211     183     140      86
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285    1038    2082 
   Size:        285     753    1044

  Fragments arranged by size: 

                1044     753     285
 
BsiEI CG_ry'CG

Cuts at:      0      80     393     408     549    1274    1910    2029    2082 
   Size:         80     313      15     141     725     636     119      53

  Fragments arranged by size: 

                 725     636     313     141     119      80      53      15
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     209     241     860    1189    1616    1852    2044    2082 
   Size:        209      32     619     329     427     236     192      38

  Fragments arranged by size: 

                 619     427     329     236     209     192      38      32
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     891    2082 
   Size:        891    1191
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     241     689     860    1189    1346    1616    1628 
   Size:        209      32     448     171     329     157     270      12

Cuts at:   1628    1852    1990    2035    2044    2082 
   Size:        224     138      45       9      38

  Fragments arranged by size: 

                 448     329     270     224     209     171     157     138
                  45      38      32      12       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1448    1724    2082 
   Size:       1448     276     358

  Fragments arranged by size: 

                1448     358     276
 
BsrBI CCG'CTC

Cuts at:      0     323     360     741     930    1532    2082 
   Size:        323      37     381     189     602     550

  Fragments arranged by size: 

                 602     550     381     323     189      37
 
BsrFI r'CCGG_y

Cuts at:      0     346     407    1171    1273    1303    1504    2082 
   Size:        346      61     764     102      30     201     578

  Fragments arranged by size: 

                 764     578     346     201     102      61      30
 
BsrGI T'GTAC_A

Cuts at:      0     272    2082 
   Size:        272    1810
 
BssHII G'CGCG_C

Cuts at:      0     336     353     704     779     819    1028    2082 
   Size:        336      17     351      75      40     209    1054

  Fragments arranged by size: 

                1054     351     336     209      75      40      17
 
BssSI C'ACGA_G

Cuts at:      0    1003    2082 
   Size:       1003    1079
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     401    1155    1856    2082 
   Size:        401     754     701     226

  Fragments arranged by size: 

                 754     701     401     226
 
BstEII G'GTnAC_C

Cuts at:      0      10    2082 
   Size:         10    2072
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96    2082 
   Size:         96    1986
 
BstYI r'GATC_y

Cuts at:      0     230    2082 
   Size:        230    1852
 
BtgI C'CryG_G

Cuts at:      0     543     576    1190    1631    1880    1904    1910    2082 
   Size:        543      33     614     441     249      24       6     172

  Fragments arranged by size: 

                 614     543     441     249     172      33      24       6
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1284    1344    2082 
   Size:       1284      60     738

  Fragments arranged by size: 

                1284     738      60
 
DraIII CAC_nnn'GTG

Cuts at:      0     440    2082 
   Size:        440    1642
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1750    1830    2025    2082 
   Size:       1750      80     195      57

  Fragments arranged by size: 

                1750     195      80      57
 
EaeI y'GGCC_r

Cuts at:      0      38      77      86     217     540     546    1223    1271 
   Size:         38      39       9     131     323       6     677      48

Cuts at:   1271    1328    1502    1907    2082 
   Size:         57     174     405     175

  Fragments arranged by size: 

                 677     405     323     175     174     131      57      48
                  39      38       9       6
 
EagI C'GGCC_G

Cuts at:      0      77     546    1271    1907    2082 
   Size:         77     469     725     636     175

  Fragments arranged by size: 

                 725     636     469     175      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     967    1483    1525    1657    2082 
   Size:        967     516      42     132     425

  Fragments arranged by size: 

                 967     516     425     132      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1582    2082 
   Size:       1582     500
 
EcoICRI GAG'CTC

Cuts at:      0    2042    2082 
   Size:       2042      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    1597    1849    2082 
   Size:        220    1377     252     233

  Fragments arranged by size: 

                1377     252     233     220
 
EcoO109I rG'GnC_Cy

Cuts at:      0     566    1713    1951    2082 
   Size:        566    1147     238     131

  Fragments arranged by size: 

                1147     566     238     131
 
HaeII r_GCGC'y

Cuts at:      0      52      63      74     619     735     915    1049    1375 
   Size:         52      11      11     545     116     180     134     326

Cuts at:   1375    1462    1842    1880    1904    2082 
   Size:         87     380      38      24     178

  Fragments arranged by size: 

                 545     380     326     180     178     134     116      87
                  52      38      24      11      11
 
KasI G'GCGC_C

Cuts at:      0     615    1458    1876    1900    2082 
   Size:        615     843     418      24     182

  Fragments arranged by size: 

                 843     615     418     182      24
 
MscI TGG'CCA

Cuts at:      0    1225    2082 
   Size:       1225     857
 
MslI CAynn'nnrTG

Cuts at:      0     154    1363    2082 
   Size:        154    1209     719

  Fragments arranged by size: 

                1209     719     154
 
MspA1I CmG'CkG

Cuts at:      0     123     222     309     539     578    1022    1464    1633 
   Size:        123      99      87     230      39     444     442     169

Cuts at:   1633    1856    1882    1912    2082 
   Size:        223      26      30     170

  Fragments arranged by size: 

                 444     442     230     223     170     169     123      99
                  87      39      30      26
 
NaeI GCC'GGC

Cuts at:      0     348    1305    1506    2082 
   Size:        348     957     201     576

  Fragments arranged by size: 

                 957     576     348     201
 
NarI GG'CG_CC

Cuts at:      0     616    1459    1877    1901    2082 
   Size:        616     843     418      24     181

  Fragments arranged by size: 

                 843     616     418     181      24
 
NgoMIV G'CCGG_C

Cuts at:      0     346    1303    1504    2082 
   Size:        346     957     201     578

  Fragments arranged by size: 

                 957     578     346     201
 
NotI GC'GGCC_GC

Cuts at:      0      77    2082 
   Size:         77    2005
 
NspI r_CATG'y

Cuts at:      0     104     159     273     399    2082 
   Size:        104      55     114     126    1683

  Fragments arranged by size: 

                1683     126     114     104      55
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1238    1431    2082 
   Size:       1238     193     651

  Fragments arranged by size: 

                1238     651     193
 
PmlI CAC'GTG

Cuts at:      0    1155    2082 
   Size:       1155     927
 
PpuMI rG'GwC_Cy

Cuts at:      0    1713    1951    2082 
   Size:       1713     238     131

  Fragments arranged by size: 

                1713     238     131
 
PstI C_TGCA'G

Cuts at:      0      59    1721    2082 
   Size:         59    1662     361

  Fragments arranged by size: 

                1662     361      59
 
SacI G_AGCT'C

Cuts at:      0    2044    2082 
   Size:       2044      38
 
SacII CC_GC'GG

Cuts at:      0     579    1634    1883    1913    2082 
   Size:        579    1055     249      30     169

  Fragments arranged by size: 

                1055     579     249     169      30
 
SanDI GG'GwC_CC

Cuts at:      0    1951    2082 
   Size:       1951     131
 
ScaI AGT'ACT

Cuts at:      0    1318    1768    2082 
   Size:       1318     450     314

  Fragments arranged by size: 

                1318     450     314
 
SfcI C'TryA_G

Cuts at:      0      55    1717    1995    2082 
   Size:         55    1662     278      87

  Fragments arranged by size: 

                1662     278      87      55
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      85    2082 
   Size:         85    1997
 
SfoI GGC'GCC

Cuts at:      0     617    1460    1878    1902    2082 
   Size:        617     843     418      24     180

  Fragments arranged by size: 

                 843     617     418     180      24
 
SmaI CCC'GGG

Cuts at:      0     690    1109    2082 
   Size:        690     419     973

  Fragments arranged by size: 

                 973     690     419
 
SmlI C'TyrA_G

Cuts at:      0     250    1762    2082 
   Size:        250    1512     320

  Fragments arranged by size: 

                1512     320     250
 
SphI G_CATG'C

Cuts at:      0     104     399    2082 
   Size:        104     295    1683

  Fragments arranged by size: 

                1683     295     104
 
SrfI GCCC'GGGC

Cuts at:      0    1109    2082 
   Size:       1109     973
 
StyI C'CwwG_G

Cuts at:      0      44      89     341    2082 
   Size:         44      45     252    1741

  Fragments arranged by size: 

                1741     252      45      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1230    1524    2082 
   Size:       1230     294     558

  Fragments arranged by size: 

                1230     558     294
 
TatI w'GTAC_w

Cuts at:      0     272    1316    1766    2082 
   Size:        272    1044     450     316

  Fragments arranged by size: 

                1044     450     316     272
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252    2082 
   Size:        220      32    1830

  Fragments arranged by size: 

                1830     220      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1365    1497    2082 
   Size:       1365     132     585

  Fragments arranged by size: 

                1365     585     132
 
XmaI C'CCGG_G

Cuts at:      0     688    1107    2082 
   Size:        688     419     975

  Fragments arranged by size: 

                 975     688     419
 
ZraI GAC'GTC

Cuts at:      0    2004    2082 
   Size:       2004      78
 

 Enzymes that do cut:

    AatII     AccI     AfeI    AlwNI    ApaLI     AscI     AvaI     BanI
    BanII     BbeI     BbsI     BglI     BlpI Bme1580I    BmgBI     BpmI
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    HaeII     KasI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NotI     NspI    PflMI     PmlI    PpuMI     PstI     SacI
    SacII    SanDI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     SrfI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BmrI     BmtI     BplI   Bpu10I    BsaBI    BsaWI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NcoI     NdeI     NheI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SalI     SapI     SbfI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmnI
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