(Linear) (Six-base) MAPSORT of: KIBB4865.seq Check: 6578 from: 1 to: 2769 KIBB4865.orf With 182 enzymes: SgfI * March 1, 2013 10:20 .. AccI GT'mk_AC Cuts at: 0 983 1262 2769 Size: 983 279 1507 Fragments arranged by size: 1507 983 279 Acc65I G'GTAC_C Cuts at: 0 110 2769 Size: 110 2659 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 2769 Size: 326 2443 AfeI AGC'GCT Cuts at: 0 913 2769 Size: 913 1856 AflIII A'CryG_T Cuts at: 0 2180 2769 Size: 2180 589 AleI CACnn'nnGTG Cuts at: 0 149 1873 2276 2769 Size: 149 1724 403 493 Fragments arranged by size: 1724 493 403 149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1891 2769 Size: 1891 878 AlwNI CAG_nnn'CTG Cuts at: 0 242 1367 2769 Size: 242 1125 1402 Fragments arranged by size: 1402 1125 242 ApoI r'AATT_y Cuts at: 0 364 416 562 854 1951 2769 Size: 364 52 146 292 1097 818 Fragments arranged by size: 1097 818 364 292 146 52 AseI AT'TA_AT Cuts at: 0 2399 2769 Size: 2399 370 AvaI C'yCGr_G Cuts at: 0 106 1696 2769 Size: 106 1590 1073 Fragments arranged by size: 1590 1073 106 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2487 2520 2769 Size: 2487 33 249 Fragments arranged by size: 2487 249 33 BanI G'GyrC_C Cuts at: 0 110 1027 1867 2769 Size: 110 917 840 902 Fragments arranged by size: 917 902 840 110 BanII G_rGCy'C Cuts at: 0 1727 1966 2131 2769 Size: 1727 239 165 638 Fragments arranged by size: 1727 638 239 165 BbeI G_GCGC'C Cuts at: 0 1031 2769 Size: 1031 1738 BbsI GAAGACnn'nnnn_ Cuts at: 0 258 332 1106 2560 2769 Size: 258 74 774 1454 209 Fragments arranged by size: 1454 774 258 209 74 BciVI GTATCCnnnnn_n' Cuts at: 0 968 2488 2769 Size: 968 1520 281 Fragments arranged by size: 1520 968 281 BclI T'GATC_A Cuts at: 0 203 1583 2769 Size: 203 1380 1186 Fragments arranged by size: 1380 1186 203 Bme1580I G_kGCm'C Cuts at: 0 1416 2598 2769 Size: 1416 1182 171 Fragments arranged by size: 1416 1182 171 BmgBI CAC'GTC Cuts at: 0 2196 2769 Size: 2196 573 BmrI ACTGGGnnnn_n' Cuts at: 0 879 1729 1995 2769 Size: 879 850 266 774 Fragments arranged by size: 879 850 774 266 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 606 1724 2769 Size: 606 1118 1045 Fragments arranged by size: 1118 1045 606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 584 601 2514 2707 2769 Size: 584 17 1913 193 62 Fragments arranged by size: 1913 584 193 62 17 Bpu10I CC'TnA_GC Cuts at: 0 1646 2769 Size: 1646 1123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1996 2769 Size: 1996 773 BsaAI yAC'GTr Cuts at: 0 258 2004 2769 Size: 258 1746 765 Fragments arranged by size: 1746 765 258 BsaBI GATnn'nnATC Cuts at: 0 714 2769 Size: 714 2055 BsaHI Gr'CG_yC Cuts at: 0 1028 2769 Size: 1028 1741 BsaWI w'CCGG_w Cuts at: 0 180 2769 Size: 180 2589 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1072 1102 2769 Size: 1072 30 1667 Fragments arranged by size: 1667 1072 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 317 1486 2251 2769 Size: 10 307 1169 765 518 Fragments arranged by size: 1169 765 518 307 10 BseYI C'CCAG_C Cuts at: 0 1409 1740 1958 2769 Size: 1409 331 218 811 Fragments arranged by size: 1409 811 331 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 413 2026 2769 Size: 413 1613 743 Fragments arranged by size: 1613 743 413 BsiEI CG_ry'CG Cuts at: 0 56 627 2769 Size: 56 571 2142 Fragments arranged by size: 2142 571 56 BsiHKAI G_wGCw'C Cuts at: 0 35 2488 2769 Size: 35 2453 281 Fragments arranged by size: 2453 281 35 BsmI GAATG_Cn' Cuts at: 0 520 722 2769 Size: 520 202 2047 Fragments arranged by size: 2047 520 202 Bsp1286I G_dGCh'C Cuts at: 0 35 1416 1727 1966 2131 2488 2598 2769 Size: 35 1381 311 239 165 357 110 171 Fragments arranged by size: 1381 357 311 239 171 165 110 35 BspEI T'CCGG_A Cuts at: 0 180 2769 Size: 180 2589 BspHI T'CATG_A Cuts at: 0 1532 2769 Size: 1532 1237 BsrBI CCG'CTC Cuts at: 0 18 2769 Size: 18 2751 BsrDI GCAATG_nn' Cuts at: 0 2550 2769 Size: 2550 219 BsrFI r'CCGG_y Cuts at: 0 55 626 2769 Size: 55 571 2143 Fragments arranged by size: 2143 571 55 BstEII G'GTnAC_C Cuts at: 0 1392 2278 2769 Size: 1392 886 491 Fragments arranged by size: 1392 886 491 BstXI CCAn_nnnn'nTGG Cuts at: 0 1878 2769 Size: 1878 891 BstYI r'GATC_y Cuts at: 0 1688 2769 Size: 1688 1081 BstZ17I GTA'TAC Cuts at: 0 1263 2769 Size: 1263 1506 BtgI C'CryG_G Cuts at: 0 731 1895 2769 Size: 731 1164 874 Fragments arranged by size: 1164 874 731 BtsI GCAGTG_nn' Cuts at: 0 807 2157 2769 Size: 807 1350 612 Fragments arranged by size: 1350 807 612 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1149 1184 2769 Size: 1149 35 1585 Fragments arranged by size: 1585 1149 35 DraIII CAC_nnn'GTG Cuts at: 0 391 463 2769 Size: 391 72 2306 Fragments arranged by size: 2306 391 72 EaeI y'GGCC_r Cuts at: 0 53 1880 2261 2769 Size: 53 1827 381 508 Fragments arranged by size: 1827 508 381 53 EagI C'GGCC_G Cuts at: 0 53 2769 Size: 53 2716 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1375 1730 2769 Size: 1375 355 1039 Fragments arranged by size: 1375 1039 355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 584 601 2514 2707 2769 Size: 326 258 17 1913 193 62 Fragments arranged by size: 1913 326 258 193 62 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 1468 2769 Size: 1468 1301 EcoO109I rG'GnC_Cy Cuts at: 0 430 2041 2189 2604 2769 Size: 430 1611 148 415 165 Fragments arranged by size: 1611 430 415 165 148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2383 2769 Size: 2383 386 HaeII r_GCGC'y Cuts at: 0 62 915 1031 2769 Size: 62 853 116 1738 Fragments arranged by size: 1738 853 116 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 162 194 309 341 600 632 1072 1104 Size: 162 32 115 32 259 32 440 32 Cuts at: 1104 1709 1741 2769 Size: 605 32 1028 Fragments arranged by size: 1028 605 440 259 162 115 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2027 2769 Size: 2027 742 HindIII A'AGCT_T Cuts at: 0 570 2769 Size: 570 2199 KasI G'GCGC_C Cuts at: 0 1027 2769 Size: 1027 1742 KpnI G_GTAC'C Cuts at: 0 114 2769 Size: 114 2655 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1115 1373 1954 2769 Size: 1115 258 581 815 Fragments arranged by size: 1115 815 581 258 MscI TGG'CCA Cuts at: 0 1882 2263 2769 Size: 1882 381 506 Fragments arranged by size: 1882 506 381 MslI CAynn'nnrTG Cuts at: 0 149 1246 1873 2276 2587 2769 Size: 149 1097 627 403 311 182 Fragments arranged by size: 1097 627 403 311 182 149 MspA1I CmG'CkG Cuts at: 0 1768 2250 2769 Size: 1768 482 519 Fragments arranged by size: 1768 519 482 NaeI GCC'GGC Cuts at: 0 57 2769 Size: 57 2712 NarI GG'CG_CC Cuts at: 0 1028 2769 Size: 1028 1741 NcoI C'CATG_G Cuts at: 0 731 2769 Size: 731 2038 NdeI CA'TA_TG Cuts at: 0 1935 2769 Size: 1935 834 NgoMIV G'CCGG_C Cuts at: 0 55 2769 Size: 55 2714 NspI r_CATG'y Cuts at: 0 2184 2769 Size: 2184 585 PasI CC'CwG_GG Cuts at: 0 2598 2769 Size: 2598 171 PciI A'CATG_T Cuts at: 0 2180 2769 Size: 2180 589 PflMI CCAn_nnn'nTGG Cuts at: 0 1936 2769 Size: 1936 833 PfoI T'CCnGG_A Cuts at: 0 557 948 2530 2769 Size: 557 391 1582 239 Fragments arranged by size: 1582 557 391 239 PmlI CAC'GTG Cuts at: 0 2004 2769 Size: 2004 765 PpuMI rG'GwC_Cy Cuts at: 0 430 2041 2189 2604 2769 Size: 430 1611 148 415 165 Fragments arranged by size: 1611 430 415 165 148 PstI C_TGCA'G Cuts at: 0 909 2769 Size: 909 1860 PvuII CAG'CTG Cuts at: 0 1768 2250 2769 Size: 1768 482 519 Fragments arranged by size: 1768 519 482 SanDI GG'GwC_CC Cuts at: 0 2041 2189 2769 Size: 2041 148 580 Fragments arranged by size: 2041 580 148 ScaI AGT'ACT Cuts at: 0 2754 2769 Size: 2754 15 SexAI A'CCwGG_T Cuts at: 0 696 2769 Size: 696 2073 SfcI C'TryA_G Cuts at: 0 783 905 1763 2769 Size: 783 122 858 1006 Fragments arranged by size: 1006 858 783 122 SfoI GGC'GCC Cuts at: 0 1029 2769 Size: 1029 1740 SmaI CCC'GGG Cuts at: 0 108 2769 Size: 108 2661 SmlI C'TyrA_G Cuts at: 0 2011 2769 Size: 2011 758 SspI AAT'ATT Cuts at: 0 1057 2769 Size: 1057 1712 StyI C'CwwG_G Cuts at: 0 731 1353 2274 2367 2769 Size: 731 622 921 93 402 Fragments arranged by size: 921 731 622 402 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 161 929 1788 2769 Size: 161 768 859 981 Fragments arranged by size: 981 859 768 161 TatI w'GTAC_w Cuts at: 0 2636 2752 2769 Size: 2636 116 17 Fragments arranged by size: 2636 116 17 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1167 1791 1861 2595 2769 Size: 1167 624 70 734 174 Fragments arranged by size: 1167 734 624 174 70 XmaI C'CCGG_G Cuts at: 0 106 2769 Size: 106 2663 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApoI AseI AvaI BaeI BanI BanII BbeI BbsI BciVI BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PstI PvuII SanDI ScaI SexAI SfcI SfoI SmaI SmlI SspI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbvCI BcgI BfrBI BglI BglII BlpI BmtI BsaI BsiWI BsmBI BspMI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI ClaI DraI DrdI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI MfeI MluI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI