################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AccIII AflII AgeI AhdI Aor51HI AscI AspEI AviII AvrII BbrPI BciVI BclI BfrI BglI BlnI BsaBI BseAI BsiWI BspDI BspEI BspHI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoT22I FbaI FseI FspI HpaI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SacI SacII SalI SexAI SfiI SfuI SgrAI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 271 GGTACC G^GTACC AclI 2872 AACGTT AA^CGTT AflIII 3078 ACATGT A^C[GA][CT]GT ApaI 4244 GGGCCC GGGCC^C AseI 3289 ATTAAT AT^TAAT AsnI 3289 ATTAAT AT^TAAT Asp700I 2533 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 271 GGTACC G^GTACC AspI 617 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 4737 CCTCAGC CCTCAGC(-5/-2) BsaAI 3532 TACGTA [CT]AC^GT[GA] BsaI 3362 GAGACC GGTCTC(1/5) Bsp1407I 4835 TGTACA T^GTACA BspLU11I 3078 ACATGT A^CATGT BsrDI 1064 CATTGC GCAATG(2/0) BsrGI 4835 TGTACA T^GTACA BssHII 4841 GCGCGC G^CGCGC BstEII 793 GGTGACC G^GT[ACGT]ACC BstPI 793 GGTGACC G^GT[ACGT]ACC DrdI 2707 GACTTCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 793 GGTGACC G^GT[ACGT]ACC EcoRI 4003 GAATTC G^AATTC EcoRV 2476 GATATC GAT^ATC KpnI 271 GGTACC GGTAC^C PaeR7I 3104 CTCGAG C^TCGAG PflFI 617 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 285 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3834 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3289 ATTAAT AT^TAAT Psp1406I 2872 AACGTT AA^CGTT SapI 575 GCTCTTC GCTCTTC(1/4) SbfI 4821 CCTGCAGG CCTGCA^GG ScaI 4988 AGTACT AGT^ACT SnaBI 3532 TACGTA TAC^GTA SphI 4688 GCATGC GCATG^C Sse8387I 4821 CCTGCAGG CCTGCA^GG SspBI 4835 TGTACA T^GTACA Tth111I 617 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 285 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 198 TCTAGA T^CTAGA XhoI 3104 CTCGAG C^TCGAG XmnI 2533 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 2745 GACGTC GACGT^C 5161 GACGTC AccI 1359 GTCTAC GT^[AC][GT]AC 4156 GTCTAC AccII 4719 CGCG CG^CG 4842 CGCG Alw44I 828 GTGCAC G^TGCAC 4315 GTGCAC AlwNI 525 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1925 CAGCTGCTG ApaLI 828 GTGCAC G^TGCAC 4315 GTGCAC BalI 3336 TGGCCA TGG^CCA 5210 TGGCCA BamHI 372 GGATCC G^GATCC 3645 GGATCC BbeI 26 GGCGCC GGCGC^C 2184 GGCGCC BlpI 891 GCTGAGC GC^T[ACGT]AGC 1129 GCTGAGC Bpu1102I 891 GCTGAGC GC^T[ACGT]AGC 1129 GCTGAGC BsaWI 238 TCCGGT [AT]^CCGG[AT] 4958 ACCGGA BsrBI 1883 GAGCGG CCGCTC(-3/-3) 2333 GAGCGG BstUI 4719 CGCG CG^CG 4842 CGCG CelII 891 GCTGAGC GC^T[ACGT]AGC 1129 GCTGAGC DraIII 4123 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 4879 CACGAGGTG EagI 159 CGGCCG C^GGCCG 3918 CGGCCG EclXI 159 CGGCCG C^GGCCG 3918 CGGCCG Eco52I 159 CGGCCG C^GGCCG 3918 CGGCCG KasI 26 GGCGCC G^GCGCC 2184 GGCGCC MluNI 3336 TGGCCA TGG^CCA 5210 TGGCCA MscI 3336 TGGCCA TGG^CCA 5210 TGGCCA MvnI 4719 CGCG CG^CG 4842 CGCG NarI 26 GGCGCC GG^CGCC 2184 GGCGCC NspI 3078 ACATGT [GA]CATG^[CT] 4688 GCATGC SfoI 26 GGCGCC GGC^GCC 2184 GGCGCC SmaI 502 CCCGGG CCC^GGG 3799 CCCGGG XmaCI 502 CCCGGG C^CCGGG 3799 CCCGGG XmaI 502 CCCGGG C^CCGGG 3799 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII