(Circular) (Six-base) MAPSORT of: pF1KE2492.seq Check: 1393 from: 1 to: 8477 pF1KE2492 8477 bp With 182 enzymes: SgfI * December 2, 2013 11:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1404 1455 5430 1404 Size: 51 3975 4451 Fragments arranged by size: 4451 3975 51 AatII G_ACGT'C Cuts at: 2857 5273 2857 Size: 2416 6061 AccI GT'mk_AC Cuts at: 1468 4265 5515 1468 Size: 2797 1250 4430 Fragments arranged by size: 4430 2797 1250 Acc65I G'GTAC_C Cuts at: 379 5493 379 Size: 5114 3363 AclI AA'CG_TT Cuts at: 2981 8317 2981 Size: 5336 3141 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 848 1169 2644 2938 3066 3904 5371 5470 6302 Size: 321 1475 294 128 838 1467 99 832 Cuts at: 6302 6734 7481 7749 848 Size: 432 747 268 1576 Fragments arranged by size: 1576 1475 1467 838 832 747 432 321 294 268 128 99 AflIII A'CryG_T Cuts at: 3186 5829 6933 7910 3186 Size: 2643 1104 977 3753 Fragments arranged by size: 3753 2643 1104 977 AgeI A'CCGG_T Cuts at: 6865 6865 Size: 8477 AleI CACnn'nnGTG Cuts at: 829 1090 1462 4204 4724 5260 829 Size: 261 372 2742 520 536 4046 Fragments arranged by size: 4046 2742 536 520 372 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1199 1199 Size: 8477 AlwNI CAG_nnn'CTG Cuts at: 638 2038 5782 7349 638 Size: 1400 3744 1567 1766 Fragments arranged by size: 3744 1766 1567 1400 ApaI G_GGCC'C Cuts at: 4356 4356 Size: 8477 ApaLI G'TGCA_C Cuts at: 936 4423 7247 936 Size: 3487 2824 2166 Fragments arranged by size: 3487 2824 2166 ApoI r'AATT_y Cuts at: 1578 1879 2758 3575 4111 4512 5481 7830 8086 Size: 301 879 817 536 401 969 2349 256 Cuts at: 8086 1578 Size: 1969 Fragments arranged by size: 2349 1969 969 879 817 536 401 301 256 AseI AT'TA_AT Cuts at: 20 3398 20 Size: 3378 5099 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8477 AvaI C'yCGr_G Cuts at: 484 610 1861 3212 3907 4428 4895 5497 484 Size: 126 1251 1351 695 521 467 602 3464 Fragments arranged by size: 3464 1351 1251 695 602 521 467 126 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3601 3634 3601 Size: 33 8444 BamHI G'GATC_C Cuts at: 480 3753 5502 480 Size: 3273 1749 3455 Fragments arranged by size: 3455 3273 1749 BanI G'GyrC_C Cuts at: 134 379 2292 2554 4000 4497 5493 5907 6155 Size: 245 1913 262 1446 497 996 414 248 Cuts at: 6155 6190 134 Size: 35 2421 Fragments arranged by size: 2421 1913 1446 996 497 414 262 248 245 35 BanII G_rGCy'C Cuts at: 632 892 2012 4356 4385 4902 5165 5491 6521 Size: 260 1120 2344 29 517 263 326 1030 Cuts at: 6521 632 Size: 2588 Fragments arranged by size: 2588 2344 1120 1030 517 326 263 260 29 BbeI G_GCGC'C Cuts at: 138 2296 5911 6159 138 Size: 2158 3615 248 2456 Fragments arranged by size: 3615 2456 2158 248 BbsI GAAGACnn'nnnn_ Cuts at: 224 2431 2849 3017 3189 3938 5273 5384 224 Size: 2207 418 168 172 749 1335 111 3317 Fragments arranged by size: 3317 2207 1335 749 418 172 168 111 BbvCI CC'TCA_GC Cuts at: 4846 4846 Size: 8477 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 403 437 917 951 2821 2855 403 Size: 34 480 34 1870 34 6025 Fragments arranged by size: 6025 1870 480 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6375 7136 8477 6375 Size: 761 1341 6375 Fragments arranged by size: 6375 1341 761 BclI T'GATC_A Cuts at: 5997 5997 Size: 8477 BglII A'GATC_T Cuts at: 1506 2946 4938 5360 5992 8029 1506 Size: 1440 1992 422 632 2037 1954 Fragments arranged by size: 2037 1992 1954 1440 632 422 BlpI GC'TnA_GC Cuts at: 1000 1238 5589 1000 Size: 238 4351 3888 Fragments arranged by size: 4351 3888 238 Bme1580I G_kGCm'C Cuts at: 940 3146 4312 4356 4427 4502 6102 6195 7251 Size: 2206 1166 44 71 75 1600 93 1056 Cuts at: 7251 940 Size: 2166 Fragments arranged by size: 2206 2166 1600 1166 1056 93 75 71 44 BmgBI CAC'GTC Cuts at: 4313 4313 Size: 8477 BmrI ACTGGGnnnn_n' Cuts at: 1408 2454 2515 3576 3816 5689 5770 6105 8249 Size: 1046 61 1061 240 1873 81 335 2144 Cuts at: 8249 1408 Size: 1636 Fragments arranged by size: 2144 1873 1636 1061 1046 335 240 81 61 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 510 804 2965 6844 510 Size: 294 2161 3879 2143 Fragments arranged by size: 3879 2161 2143 294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 232 421 1574 2168 3488 3779 4354 5077 5179 Size: 189 1153 594 1320 291 575 723 102 Cuts at: 5179 232 Size: 3530 Fragments arranged by size: 3530 1320 1153 723 594 575 291 189 102 Bpu10I CC'TnA_GC Cuts at: 631 1200 4846 631 Size: 569 3646 4262 Fragments arranged by size: 4262 3646 569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1062 1941 5101 5377 5653 6532 7024 7322 1062 Size: 879 3160 276 276 879 492 298 2217 Fragments arranged by size: 3160 2217 879 879 492 298 276 276 BsaI GGTCTCn'nnnn_ Cuts at: 3464 8181 3464 Size: 4717 3760 BsaAI yAC'GTr Cuts at: 3642 5830 3642 Size: 2188 6289 BsaBI GATnn'nnATC Cuts at: 6 5991 8028 6 Size: 5985 2037 455 Fragments arranged by size: 5985 2037 455 BsaHI Gr'CG_yC Cuts at: 135 2293 2500 2854 5270 5908 6156 6858 8354 Size: 2158 207 354 2416 638 248 702 1496 Cuts at: 8354 135 Size: 258 Fragments arranged by size: 2416 2158 1496 702 638 354 258 248 207 BsaWI w'CCGG_w Cuts at: 346 5066 5666 6187 6865 7139 7286 7697 346 Size: 4720 600 521 678 274 147 411 1126 Fragments arranged by size: 4720 1126 678 600 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 847 877 1473 1503 4007 4037 847 Size: 30 596 30 2504 30 5287 Fragments arranged by size: 5287 2504 596 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 298 807 852 1374 2398 2428 2453 4845 298 Size: 509 45 522 1024 30 25 2392 3930 Fragments arranged by size: 3930 2392 1024 522 509 45 30 25 BseYI C'CCAG_C Cuts at: 771 1133 1307 2038 2191 3920 4955 5054 5188 Size: 362 174 731 153 1729 1035 99 134 Cuts at: 5188 5192 5902 6660 7237 7733 771 Size: 4 710 758 577 496 1515 Fragments arranged by size: 1729 1515 1035 758 731 710 577 496 362 174 153 134 99 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 168 318 1438 2293 3211 168 Size: 150 1120 855 918 5434 Fragments arranged by size: 5434 1120 918 855 150 BsiEI CG_ry'CG Cuts at: 104 270 4029 5067 6019 6065 7273 104 Size: 166 3759 1038 952 46 1208 1308 Fragments arranged by size: 3759 1308 1208 1038 952 166 46 BsiHKAI G_wGCw'C Cuts at: 940 2696 2873 3589 4201 4232 4427 5093 5201 Size: 1756 177 716 612 31 195 666 108 Cuts at: 5201 5491 6269 6459 7251 940 Size: 290 778 190 792 2166 Fragments arranged by size: 2166 1756 792 778 716 666 612 290 195 190 177 108 31 BsmI GAATG_Cn' Cuts at: 1601 1905 3769 1601 Size: 304 1864 6309 Fragments arranged by size: 6309 1864 304 BsmBI CGTCTCn'nnnn_ Cuts at: 613 3535 3835 613 Size: 2922 300 5255 Fragments arranged by size: 5255 2922 300 Bsp1286I G_dGCh'C Cuts at: 632 892 940 2012 2696 2873 3146 3589 4201 Size: 260 48 1072 684 177 273 443 612 Cuts at: 4201 4232 4312 4356 4385 4427 4502 4902 5093 Size: 31 80 44 29 42 75 400 191 Cuts at: 5093 5165 5201 5491 6102 6195 6269 6459 6521 Size: 72 36 290 611 93 74 190 62 Cuts at: 6521 7251 632 Size: 730 1858 Fragments arranged by size: 1858 1072 730 684 612 611 443 400 290 273 260 191 190 177 93 80 75 74 72 62 48 44 42 36 31 29 BspEI T'CCGG_A Cuts at: 7697 7697 Size: 8477 BspHI T'CATG_A Cuts at: 7653 7653 Size: 8477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1404 1455 5430 5527 5741 6043 6424 1404 Size: 51 3975 97 214 302 381 3457 Fragments arranged by size: 3975 3457 381 302 214 97 51 BsrBI CCG'CTC Cuts at: 50 1993 2443 6769 6823 8307 8472 50 Size: 1943 450 4326 54 1484 165 55 Fragments arranged by size: 4326 1943 1484 450 165 55 54 BsrDI GCAATG_nn' Cuts at: 1171 6389 1171 Size: 5218 3259 BsrFI r'CCGG_y Cuts at: 643 2295 2704 4246 4283 6475 6656 6865 643 Size: 1652 409 1542 37 2192 181 209 2255 Fragments arranged by size: 2255 2192 1652 1542 409 209 181 37 BsrGI T'GTAC_A Cuts at: 4943 4943 Size: 8477 BssHII G'CGCG_C Cuts at: 4949 4949 Size: 8477 BssSI C'ACGA_G Cuts at: 1350 4720 4987 5260 6748 7106 1350 Size: 3370 267 273 1488 358 2721 Fragments arranged by size: 3370 2721 1488 358 273 267 BstAPI GCAn_nnn'nTGC Cuts at: 5854 5854 Size: 8477 BstBI TT'CG_AA Cuts at: 6838 6838 Size: 8477 BstEII G'GTnAC_C Cuts at: 901 901 Size: 8477 BstXI CCAn_nnnn'nTGG Cuts at: 138 2577 3204 3466 138 Size: 2439 627 262 5149 Fragments arranged by size: 5149 2439 627 262 BstYI r'GATC_y Cuts at: 1 480 1506 2946 3753 4806 4938 5360 5502 Size: 479 1026 1440 807 1053 132 422 142 Cuts at: 5502 5969 5992 6327 6573 7585 7671 7683 8029 Size: 467 23 335 246 1012 86 12 346 Cuts at: 8029 1 Size: 449 Fragments arranged by size: 1440 1053 1026 1012 807 479 467 449 422 346 335 246 142 132 86 23 12 Bsu36I CC'TnA_GG Cuts at: 920 4546 4764 920 Size: 3626 218 4633 Fragments arranged by size: 4633 3626 218 BtgI C'CryG_G Cuts at: 354 383 835 1481 1886 2861 4029 4088 4502 Size: 29 452 646 405 975 1168 59 414 Cuts at: 4502 4562 354 Size: 60 4269 Fragments arranged by size: 4269 1168 975 646 452 414 405 60 59 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1146 2149 5092 5196 6427 6608 6771 1146 Size: 1003 2943 104 1231 181 163 2852 Fragments arranged by size: 2943 2852 1231 1003 181 163 104 BtsI GCAGTG_nn' Cuts at: 3261 4211 4405 5720 3261 Size: 950 194 1315 6018 Fragments arranged by size: 6018 1315 950 194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 4450 4485 4863 4898 4993 5028 4450 Size: 35 378 35 95 35 7899 Fragments arranged by size: 7899 378 95 35 35 35 DraI TTT'AAA Cuts at: 5476 5476 Size: 8477 DraIII CAC_nnn'GTG Cuts at: 4236 4992 4236 Size: 756 7721 DrdI GACnn_nn'nnGTC Cuts at: 2821 6183 7041 2821 Size: 3362 858 4257 Fragments arranged by size: 4257 3362 858 EaeI y'GGCC_r Cuts at: 267 1274 2297 2444 2864 3444 4026 5306 5318 Size: 1007 1023 147 420 580 582 1280 12 Cuts at: 5318 6062 6236 6627 6654 7999 267 Size: 744 174 391 27 1345 745 Fragments arranged by size: 1345 1280 1023 1007 745 744 582 580 420 391 174 147 27 12 EagI C'GGCC_G Cuts at: 267 4026 6062 267 Size: 3759 2036 2682 Fragments arranged by size: 3759 2682 2036 EarI CTCTTCn'nnn_ Cuts at: 275 572 690 1145 1815 1937 3880 4436 5065 Size: 297 118 455 670 122 1943 556 629 Cuts at: 5065 6500 6710 275 Size: 1435 210 2042 Fragments arranged by size: 2042 1943 1435 670 629 556 455 297 210 122 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 5031 6685 6995 7141 8287 5031 Size: 1654 310 146 1146 5221 Fragments arranged by size: 5221 1654 1146 310 146 EcoICRI GAG'CTC Cuts at: 5489 5489 Size: 8477 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 232 421 848 1169 1574 2168 2644 2938 3066 Size: 189 427 321 405 594 476 294 128 Cuts at: 3066 3488 3779 3904 4354 5077 5179 5371 5470 Size: 422 291 125 450 723 102 192 99 Cuts at: 5470 6302 6734 7481 7749 232 Size: 832 432 747 268 960 Fragments arranged by size: 960 832 747 723 594 476 450 432 427 422 405 321 294 291 268 192 189 128 125 102 99 EcoNI CCTnn'n_nnAGG Cuts at: 211 871 3150 4767 211 Size: 660 2279 1617 3921 Fragments arranged by size: 3921 2279 1617 660 EcoO109I rG'GnC_Cy Cuts at: 635 1124 1730 2175 4352 4587 5616 635 Size: 489 606 445 2177 235 1029 3496 Fragments arranged by size: 3496 2177 1029 606 489 445 235 EcoRI G'AATT_C Cuts at: 4111 5481 4111 Size: 1370 7107 EcoRV GAT'ATC Cuts at: 2586 2586 Size: 8477 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1305 2968 6496 1305 Size: 1663 3528 3286 Fragments arranged by size: 3528 3286 1663 FspI TGC'GCA Cuts at: 6258 6258 Size: 8477 HaeII r_GCGC'y Cuts at: 138 2296 2898 3140 3226 4321 5911 6159 7181 Size: 2158 602 242 86 1095 1590 248 1022 Cuts at: 7181 8160 138 Size: 979 455 Fragments arranged by size: 2158 1590 1095 1022 979 602 455 248 242 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 465 490 497 522 4007 4039 4720 4752 5195 Size: 25 7 25 3485 32 681 32 443 Cuts at: 5195 5227 6319 6349 6351 6381 465 Size: 32 1092 30 2 30 2561 Fragments arranged by size: 3485 2561 1092 681 443 32 32 32 30 30 25 25 7 2 HincII GTy'rAC Cuts at: 3108 4581 4977 5516 3108 Size: 1473 396 539 6069 Fragments arranged by size: 6069 1473 539 396 HindIII A'AGCT_T Cuts at: 1269 1616 3433 5353 1269 Size: 347 1817 1920 4393 Fragments arranged by size: 4393 1920 1817 347 KasI G'GCGC_C Cuts at: 134 2292 5907 6155 134 Size: 2158 3615 248 2456 Fragments arranged by size: 3615 2456 2158 248 KpnI G_GTAC'C Cuts at: 383 5497 383 Size: 5114 3363 MluI A'CGCG_T Cuts at: 7910 7910 Size: 8477 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 713 2609 3053 3059 4620 5087 7148 7332 713 Size: 1896 444 6 1561 467 2061 184 1858 Fragments arranged by size: 2061 1896 1858 1561 467 444 184 6 MscI TGG'CCA Cuts at: 3446 5320 6238 3446 Size: 1874 918 5685 Fragments arranged by size: 5685 1874 918 MslI CAynn'nnrTG Cuts at: 674 829 1090 1462 2404 3410 4204 4567 4724 Size: 155 261 372 942 1006 794 363 157 Cuts at: 4724 5179 5227 5260 6466 6593 674 Size: 455 48 33 1206 127 2558 Fragments arranged by size: 2558 1206 1006 942 794 455 372 363 261 157 155 127 48 33 MspA1I CmG'CkG Cuts at: 453 1090 2035 3693 4651 5054 5192 5304 5588 Size: 637 945 1658 958 403 138 112 284 Cuts at: 5588 5902 6262 7275 7520 453 Size: 314 360 1013 245 1410 Fragments arranged by size: 1658 1410 1013 958 945 637 403 360 314 284 245 138 112 NaeI GCC'GGC Cuts at: 2297 4248 4285 6658 2297 Size: 1951 37 2373 4116 Fragments arranged by size: 4116 2373 1951 37 NarI GG'CG_CC Cuts at: 135 2293 5908 6156 135 Size: 2158 3615 248 2456 Fragments arranged by size: 3615 2456 2158 248 NcoI C'CATG_G Cuts at: 383 1886 4562 383 Size: 1503 2676 4298 Fragments arranged by size: 4298 2676 1503 NgoMIV G'CCGG_C Cuts at: 2295 4246 4283 6656 2295 Size: 1951 37 2373 4116 Fragments arranged by size: 4116 2373 1951 37 NspI r_CATG'y Cuts at: 3190 4800 5530 6030 6937 3190 Size: 1610 730 500 907 4730 Fragments arranged by size: 4730 1610 907 730 500 PciI A'CATG_T Cuts at: 3186 6933 3186 Size: 3747 4730 PflMI CCAn_nnn'nTGG Cuts at: 399 399 Size: 8477 PfoI T'CCnGG_A Cuts at: 523 883 2074 3756 4007 4106 4809 5330 523 Size: 360 1191 1682 251 99 703 521 3670 Fragments arranged by size: 3670 1682 1191 703 521 360 251 99 PmeI GTTT'AAAC Cuts at: 5476 5476 Size: 8477 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7616 7648 7616 Size: 32 8445 PpuMI rG'GwC_Cy Cuts at: 635 1124 1730 635 Size: 489 606 7382 Fragments arranged by size: 7382 606 489 PshAI GACnn'nnGTC Cuts at: 3946 3946 Size: 8477 PsiI TTA'TAA Cuts at: 2780 2780 Size: 8477 PspOMI G'GGCC_C Cuts at: 4352 4352 Size: 8477 PspXI vC'TCGA_Gb Cuts at: 3212 3212 Size: 8477 PstI C_TGCA'G Cuts at: 872 1118 4400 4934 5524 6209 872 Size: 246 3282 534 590 685 3140 Fragments arranged by size: 3282 3140 685 590 534 246 PvuI CG_AT'CG Cuts at: 104 104 Size: 8477 PvuII CAG'CTG Cuts at: 453 1090 2035 3693 5192 5902 6262 453 Size: 637 945 1658 1499 710 360 2668 Fragments arranged by size: 2668 1658 1499 945 710 637 360 RsrII CG'GwC_CG Cuts at: 6672 6672 Size: 8477 SacI G_AGCT'C Cuts at: 5491 5491 Size: 8477 SalI G'TCGA_C Cuts at: 5514 5514 Size: 8477 SapI GCTCTTCn'nnn_ Cuts at: 690 6500 6710 690 Size: 5810 210 2457 Fragments arranged by size: 5810 2457 210 SbfI CC_TGCA'GG Cuts at: 4934 5524 4934 Size: 590 7887 ScaI AGT'ACT Cuts at: 5098 7994 5098 Size: 2896 5581 SfcI C'TryA_G Cuts at: 33 868 1114 4119 4396 4536 4930 5520 6205 Size: 835 246 3005 277 140 394 590 685 Cuts at: 6205 7198 7389 33 Size: 993 191 1121 Fragments arranged by size: 3005 1121 993 835 685 590 394 277 246 191 140 SfoI GGC'GCC Cuts at: 136 2294 5909 6157 136 Size: 2158 3615 248 2456 Fragments arranged by size: 3615 2456 2158 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8477 SmaI CCC'GGG Cuts at: 612 3909 5499 612 Size: 3297 1590 3590 Fragments arranged by size: 3590 3297 1590 SmlI C'TyrA_G Cuts at: 1077 1920 3212 5116 5356 5632 6547 7039 7301 Size: 843 1292 1904 240 276 915 492 262 Cuts at: 7301 1077 Size: 2253 Fragments arranged by size: 2253 1904 1292 915 843 492 276 262 240 SnaBI TAC'GTA Cuts at: 3642 3642 Size: 8477 SpeI A'CTAG_T Cuts at: 67 67 Size: 8477 SphI G_CATG'C Cuts at: 4800 5530 6030 4800 Size: 730 500 7247 Fragments arranged by size: 7247 730 500 StuI AGG'CCT Cuts at: 1180 1198 2419 2908 3316 5465 1180 Size: 18 1221 489 408 2149 4192 Fragments arranged by size: 4192 2149 1221 489 408 18 StyI C'CwwG_G Cuts at: 383 653 1205 1886 4562 5611 5965 383 Size: 270 552 681 2676 1049 354 2895 Fragments arranged by size: 2895 2676 1049 681 552 354 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 406 631 664 1147 1870 2205 2329 2547 2859 Size: 225 33 483 723 335 124 218 312 Cuts at: 2859 3430 6060 6193 6652 6861 406 Size: 571 2630 133 459 209 2022 Fragments arranged by size: 2630 2022 723 571 483 459 335 312 225 218 209 133 124 33 TatI w'GTAC_w Cuts at: 4478 4943 5096 7992 4478 Size: 465 153 2896 4963 Fragments arranged by size: 4963 2896 465 153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 847 879 1473 1505 7994 8026 847 Size: 32 594 32 6489 32 1298 Fragments arranged by size: 6489 1298 594 32 32 32 Tth111I GACn'n_nGTC Cuts at: 728 6274 728 Size: 5546 2931 XbaI T'CTAG_A Cuts at: 306 5508 306 Size: 5202 3275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1152 1269 3609 4086 4560 4751 5301 5972 1152 Size: 117 2340 477 474 191 550 671 3657 Fragments arranged by size: 3657 2340 671 550 477 474 191 117 XhoI C'TCGA_G Cuts at: 3212 3212 Size: 8477 XmaI C'CCGG_G Cuts at: 610 3907 5497 610 Size: 3297 1590 3590 Fragments arranged by size: 3590 3297 1590 XmnI GAAnn'nnTTC Cuts at: 2645 2645 Size: 8477 ZraI GAC'GTC Cuts at: 2855 5271 2855 Size: 2416 6061 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AhdI AloI AscI AvrII BfrBI BglI BmtI BsiWI BstZ17I ClaI FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI