(Linear) (Six-base) MAPSORT of: KIEE2492.seq Check: 3513 from: 1 to: 5364 KIEE2492.orf With 182 enzymes: SgfI * December 2, 2013 11:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1296 1347 5322 5364 Size: 1296 51 3975 42 Fragments arranged by size: 3975 1296 51 42 AatII G_ACGT'C Cuts at: 0 2749 5165 5364 Size: 2749 2416 199 Fragments arranged by size: 2749 2416 199 AccI GT'mk_AC Cuts at: 0 1360 4157 5364 Size: 1360 2797 1207 Fragments arranged by size: 2797 1360 1207 Acc65I G'GTAC_C Cuts at: 0 271 5364 Size: 271 5093 AclI AA'CG_TT Cuts at: 0 2873 5364 Size: 2873 2491 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 740 1061 2536 2830 2958 3796 5263 5362 Size: 740 321 1475 294 128 838 1467 99 Cuts at: 5362 5364 Size: 2 Fragments arranged by size: 1475 1467 838 740 321 294 128 99 2 AflIII A'CryG_T Cuts at: 0 3078 5364 Size: 3078 2286 AleI CACnn'nnGTG Cuts at: 0 721 982 1354 4096 4616 5152 5364 Size: 721 261 372 2742 520 536 212 Fragments arranged by size: 2742 721 536 520 372 261 212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1091 5364 Size: 1091 4273 AlwNI CAG_nnn'CTG Cuts at: 0 530 1930 5364 Size: 530 1400 3434 Fragments arranged by size: 3434 1400 530 ApaI G_GGCC'C Cuts at: 0 4248 5364 Size: 4248 1116 ApaLI G'TGCA_C Cuts at: 0 828 4315 5364 Size: 828 3487 1049 Fragments arranged by size: 3487 1049 828 ApoI r'AATT_y Cuts at: 0 1470 1771 2650 3467 4003 4404 5364 Size: 1470 301 879 817 536 401 960 Fragments arranged by size: 1470 960 879 817 536 401 301 AseI AT'TA_AT Cuts at: 0 3290 5364 Size: 3290 2074 AvaI C'yCGr_G Cuts at: 0 376 502 1753 3104 3799 4320 4787 5364 Size: 376 126 1251 1351 695 521 467 577 Fragments arranged by size: 1351 1251 695 577 521 467 376 126 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3493 3526 5364 Size: 3493 33 1838 Fragments arranged by size: 3493 1838 33 BamHI G'GATC_C Cuts at: 0 372 3645 5364 Size: 372 3273 1719 Fragments arranged by size: 3273 1719 372 BanI G'GyrC_C Cuts at: 0 26 271 2184 2446 3892 4389 5364 Size: 26 245 1913 262 1446 497 975 Fragments arranged by size: 1913 1446 975 497 262 245 26 BanII G_rGCy'C Cuts at: 0 524 784 1904 4248 4277 4794 5057 5364 Size: 524 260 1120 2344 29 517 263 307 Fragments arranged by size: 2344 1120 524 517 307 263 260 29 BbeI G_GCGC'C Cuts at: 0 30 2188 5364 Size: 30 2158 3176 Fragments arranged by size: 3176 2158 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 116 2323 2741 2909 3081 3830 5165 5276 Size: 116 2207 418 168 172 749 1335 111 Cuts at: 5276 5364 Size: 88 Fragments arranged by size: 2207 1335 749 418 172 168 116 111 88 BbvCI CC'TCA_GC Cuts at: 0 4738 5364 Size: 4738 626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 329 809 843 2713 2747 5364 Size: 295 34 480 34 1870 34 2617 Fragments arranged by size: 2617 1870 480 295 34 34 34 BglII A'GATC_T Cuts at: 0 1398 2838 4830 5252 5364 Size: 1398 1440 1992 422 112 Fragments arranged by size: 1992 1440 1398 422 112 BlpI GC'TnA_GC Cuts at: 0 892 1130 5364 Size: 892 238 4234 Fragments arranged by size: 4234 892 238 Bme1580I G_kGCm'C Cuts at: 0 832 3038 4204 4248 4319 4394 5364 Size: 832 2206 1166 44 71 75 970 Fragments arranged by size: 2206 1166 970 832 75 71 44 BmgBI CAC'GTC Cuts at: 0 4205 5364 Size: 4205 1159 BmrI ACTGGGnnnn_n' Cuts at: 0 1300 2346 2407 3468 3708 5364 Size: 1300 1046 61 1061 240 1656 Fragments arranged by size: 1656 1300 1061 1046 240 61 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 402 696 2857 5364 Size: 402 294 2161 2507 Fragments arranged by size: 2507 2161 402 294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 313 1466 2060 3380 3671 4246 4969 Size: 124 189 1153 594 1320 291 575 723 Cuts at: 4969 5071 5364 Size: 102 293 Fragments arranged by size: 1320 1153 723 594 575 293 291 189 124 102 Bpu10I CC'TnA_GC Cuts at: 0 523 1092 4738 5364 Size: 523 569 3646 626 Fragments arranged by size: 3646 626 569 523 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 954 1833 4993 5269 5364 Size: 954 879 3160 276 95 Fragments arranged by size: 3160 954 879 276 95 BsaI GGTCTCn'nnnn_ Cuts at: 0 3356 5364 Size: 3356 2008 BsaAI yAC'GTr Cuts at: 0 3534 5364 Size: 3534 1830 BsaHI Gr'CG_yC Cuts at: 0 27 2185 2392 2746 5162 5364 Size: 27 2158 207 354 2416 202 Fragments arranged by size: 2416 2158 354 207 202 27 BsaWI w'CCGG_w Cuts at: 0 238 4958 5364 Size: 238 4720 406 Fragments arranged by size: 4720 406 238 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 739 769 1365 1395 3899 3929 5364 Size: 739 30 596 30 2504 30 1435 Fragments arranged by size: 2504 1435 739 596 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 190 699 744 1266 2290 2320 2345 4737 Size: 190 509 45 522 1024 30 25 2392 Cuts at: 4737 5364 Size: 627 Fragments arranged by size: 2392 1024 627 522 509 190 45 30 25 BseYI C'CCAG_C Cuts at: 0 663 1025 1199 1930 2083 3812 4847 4946 Size: 663 362 174 731 153 1729 1035 99 Cuts at: 4946 5080 5084 5364 Size: 134 4 280 Fragments arranged by size: 1729 1035 731 663 362 280 174 153 134 99 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 210 1330 2185 3103 5364 Size: 60 150 1120 855 918 2261 Fragments arranged by size: 2261 1120 918 855 150 60 BsiEI CG_ry'CG Cuts at: 0 162 3921 4959 5364 Size: 162 3759 1038 405 Fragments arranged by size: 3759 1038 405 162 BsiHKAI G_wGCw'C Cuts at: 0 832 2588 2765 3481 4093 4124 4319 4985 Size: 832 1756 177 716 612 31 195 666 Cuts at: 4985 5093 5364 Size: 108 271 Fragments arranged by size: 1756 832 716 666 612 271 195 177 108 31 BsmI GAATG_Cn' Cuts at: 0 1493 1797 3661 5364 Size: 1493 304 1864 1703 Fragments arranged by size: 1864 1703 1493 304 BsmBI CGTCTCn'nnnn_ Cuts at: 0 505 3427 3727 5364 Size: 505 2922 300 1637 Fragments arranged by size: 2922 1637 505 300 Bsp1286I G_dGCh'C Cuts at: 0 524 784 832 1904 2588 2765 3038 3481 Size: 524 260 48 1072 684 177 273 443 Cuts at: 3481 4093 4124 4204 4248 4277 4319 4394 4794 Size: 612 31 80 44 29 42 75 400 Cuts at: 4794 4985 5057 5093 5364 Size: 191 72 36 271 Fragments arranged by size: 1072 684 612 524 443 400 273 271 260 191 177 80 75 72 48 44 42 36 31 29 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1296 1347 5322 5364 Size: 1296 51 3975 42 Fragments arranged by size: 3975 1296 51 42 BsrBI CCG'CTC Cuts at: 0 1885 2335 5364 Size: 1885 450 3029 Fragments arranged by size: 3029 1885 450 BsrDI GCAATG_nn' Cuts at: 0 1063 5364 Size: 1063 4301 BsrFI r'CCGG_y Cuts at: 0 535 2187 2596 4138 4175 5364 Size: 535 1652 409 1542 37 1189 Fragments arranged by size: 1652 1542 1189 535 409 37 BsrGI T'GTAC_A Cuts at: 0 4835 5364 Size: 4835 529 BssHII G'CGCG_C Cuts at: 0 4841 5364 Size: 4841 523 BssSI C'ACGA_G Cuts at: 0 1242 4612 4879 5152 5364 Size: 1242 3370 267 273 212 Fragments arranged by size: 3370 1242 273 267 212 BstEII G'GTnAC_C Cuts at: 0 793 5364 Size: 793 4571 BstXI CCAn_nnnn'nTGG Cuts at: 0 30 2469 3096 3358 5364 Size: 30 2439 627 262 2006 Fragments arranged by size: 2439 2006 627 262 30 BstYI r'GATC_y Cuts at: 0 372 1398 2838 3645 4698 4830 5252 5364 Size: 372 1026 1440 807 1053 132 422 112 Fragments arranged by size: 1440 1053 1026 807 422 372 132 112 Bsu36I CC'TnA_GG Cuts at: 0 812 4438 4656 5364 Size: 812 3626 218 708 Fragments arranged by size: 3626 812 708 218 BtgI C'CryG_G Cuts at: 0 246 275 727 1373 1778 2753 3921 3980 Size: 246 29 452 646 405 975 1168 59 Cuts at: 3980 4394 4454 5364 Size: 414 60 910 Fragments arranged by size: 1168 975 910 646 452 414 405 246 60 59 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1038 2041 4984 5088 5364 Size: 1038 1003 2943 104 276 Fragments arranged by size: 2943 1038 1003 276 104 BtsI GCAGTG_nn' Cuts at: 0 3153 4103 4297 5364 Size: 3153 950 194 1067 Fragments arranged by size: 3153 1067 950 194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 4342 4377 4755 4790 4885 4920 5364 Size: 4342 35 378 35 95 35 444 Fragments arranged by size: 4342 444 378 95 35 35 35 DraIII CAC_nnn'GTG Cuts at: 0 4128 4884 5364 Size: 4128 756 480 Fragments arranged by size: 4128 756 480 DrdI GACnn_nn'nnGTC Cuts at: 0 2713 5364 Size: 2713 2651 EaeI y'GGCC_r Cuts at: 0 159 1166 2189 2336 2756 3336 3918 5198 Size: 159 1007 1023 147 420 580 582 1280 Cuts at: 5198 5210 5364 Size: 12 154 Fragments arranged by size: 1280 1023 1007 582 580 420 159 154 147 12 EagI C'GGCC_G Cuts at: 0 159 3918 5364 Size: 159 3759 1446 Fragments arranged by size: 3759 1446 159 EarI CTCTTCn'nnn_ Cuts at: 0 167 464 582 1037 1707 1829 3772 4328 Size: 167 297 118 455 670 122 1943 556 Cuts at: 4328 4957 5364 Size: 629 407 Fragments arranged by size: 1943 670 629 556 455 407 297 167 122 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4923 5364 Size: 4923 441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 313 740 1061 1466 2060 2536 2830 Size: 124 189 427 321 405 594 476 294 Cuts at: 2830 2958 3380 3671 3796 4246 4969 5071 5263 Size: 128 422 291 125 450 723 102 192 Cuts at: 5263 5362 5364 Size: 99 2 Fragments arranged by size: 723 594 476 450 427 422 405 321 294 291 192 189 128 125 124 102 99 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 103 763 3042 4659 5364 Size: 103 660 2279 1617 705 Fragments arranged by size: 2279 1617 705 660 103 EcoO109I rG'GnC_Cy Cuts at: 0 527 1016 1622 2067 4244 4479 5364 Size: 527 489 606 445 2177 235 885 Fragments arranged by size: 2177 885 606 527 489 445 235 EcoRI G'AATT_C Cuts at: 0 4003 5364 Size: 4003 1361 EcoRV GAT'ATC Cuts at: 0 2478 5364 Size: 2478 2886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1197 2860 5364 Size: 1197 1663 2504 Fragments arranged by size: 2504 1663 1197 HaeII r_GCGC'y Cuts at: 0 30 2188 2790 3032 3118 4213 5364 Size: 30 2158 602 242 86 1095 1151 Fragments arranged by size: 2158 1151 1095 602 242 86 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 357 382 389 414 3899 3931 4612 4644 Size: 357 25 7 25 3485 32 681 32 Cuts at: 4644 5087 5119 5364 Size: 443 32 245 Fragments arranged by size: 3485 681 443 357 245 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 0 3000 4473 4869 5364 Size: 3000 1473 396 495 Fragments arranged by size: 3000 1473 495 396 HindIII A'AGCT_T Cuts at: 0 1161 1508 3325 5245 5364 Size: 1161 347 1817 1920 119 Fragments arranged by size: 1920 1817 1161 347 119 KasI G'GCGC_C Cuts at: 0 26 2184 5364 Size: 26 2158 3180 Fragments arranged by size: 3180 2158 26 KpnI G_GTAC'C Cuts at: 0 275 5364 Size: 275 5089 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 605 2501 2945 2951 4512 4979 5364 Size: 605 1896 444 6 1561 467 385 Fragments arranged by size: 1896 1561 605 467 444 385 6 MscI TGG'CCA Cuts at: 0 3338 5212 5364 Size: 3338 1874 152 Fragments arranged by size: 3338 1874 152 MslI CAynn'nnrTG Cuts at: 0 566 721 982 1354 2296 3302 4096 4459 Size: 566 155 261 372 942 1006 794 363 Cuts at: 4459 4616 5071 5119 5152 5364 Size: 157 455 48 33 212 Fragments arranged by size: 1006 942 794 566 455 372 363 261 212 157 155 48 33 MspA1I CmG'CkG Cuts at: 0 345 982 1927 3585 4543 4946 5084 5196 Size: 345 637 945 1658 958 403 138 112 Cuts at: 5196 5364 Size: 168 Fragments arranged by size: 1658 958 945 637 403 345 168 138 112 NaeI GCC'GGC Cuts at: 0 2189 4140 4177 5364 Size: 2189 1951 37 1187 Fragments arranged by size: 2189 1951 1187 37 NarI GG'CG_CC Cuts at: 0 27 2185 5364 Size: 27 2158 3179 Fragments arranged by size: 3179 2158 27 NcoI C'CATG_G Cuts at: 0 275 1778 4454 5364 Size: 275 1503 2676 910 Fragments arranged by size: 2676 1503 910 275 NgoMIV G'CCGG_C Cuts at: 0 2187 4138 4175 5364 Size: 2187 1951 37 1189 Fragments arranged by size: 2187 1951 1189 37 NspI r_CATG'y Cuts at: 0 3082 4692 5364 Size: 3082 1610 672 Fragments arranged by size: 3082 1610 672 PciI A'CATG_T Cuts at: 0 3078 5364 Size: 3078 2286 PflMI CCAn_nnn'nTGG Cuts at: 0 291 5364 Size: 291 5073 PfoI T'CCnGG_A Cuts at: 0 415 775 1966 3648 3899 3998 4701 5222 Size: 415 360 1191 1682 251 99 703 521 Cuts at: 5222 5364 Size: 142 Fragments arranged by size: 1682 1191 703 521 415 360 251 142 99 PpuMI rG'GwC_Cy Cuts at: 0 527 1016 1622 5364 Size: 527 489 606 3742 Fragments arranged by size: 3742 606 527 489 PshAI GACnn'nnGTC Cuts at: 0 3838 5364 Size: 3838 1526 PsiI TTA'TAA Cuts at: 0 2672 5364 Size: 2672 2692 PspOMI G'GGCC_C Cuts at: 0 4244 5364 Size: 4244 1120 PspXI vC'TCGA_Gb Cuts at: 0 3104 5364 Size: 3104 2260 PstI C_TGCA'G Cuts at: 0 764 1010 4292 4826 5364 Size: 764 246 3282 534 538 Fragments arranged by size: 3282 764 538 534 246 PvuII CAG'CTG Cuts at: 0 345 982 1927 3585 5084 5364 Size: 345 637 945 1658 1499 280 Fragments arranged by size: 1658 1499 945 637 345 280 SapI GCTCTTCn'nnn_ Cuts at: 0 582 5364 Size: 582 4782 SbfI CC_TGCA'GG Cuts at: 0 4826 5364 Size: 4826 538 ScaI AGT'ACT Cuts at: 0 4990 5364 Size: 4990 374 SfcI C'TryA_G Cuts at: 0 760 1006 4011 4288 4428 4822 5364 Size: 760 246 3005 277 140 394 542 Fragments arranged by size: 3005 760 542 394 277 246 140 SfoI GGC'GCC Cuts at: 0 28 2186 5364 Size: 28 2158 3178 Fragments arranged by size: 3178 2158 28 SmaI CCC'GGG Cuts at: 0 504 3801 5364 Size: 504 3297 1563 Fragments arranged by size: 3297 1563 504 SmlI C'TyrA_G Cuts at: 0 969 1812 3104 5008 5248 5364 Size: 969 843 1292 1904 240 116 Fragments arranged by size: 1904 1292 969 843 240 116 SnaBI TAC'GTA Cuts at: 0 3534 5364 Size: 3534 1830 SphI G_CATG'C Cuts at: 0 4692 5364 Size: 4692 672 StuI AGG'CCT Cuts at: 0 1072 1090 2311 2800 3208 5357 5364 Size: 1072 18 1221 489 408 2149 7 Fragments arranged by size: 2149 1221 1072 489 408 18 7 StyI C'CwwG_G Cuts at: 0 275 545 1097 1778 4454 5364 Size: 275 270 552 681 2676 910 Fragments arranged by size: 2676 910 681 552 275 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 298 523 556 1039 1762 2097 2221 2439 Size: 298 225 33 483 723 335 124 218 Cuts at: 2439 2751 3322 5364 Size: 312 571 2042 Fragments arranged by size: 2042 723 571 483 335 312 298 225 218 124 33 TatI w'GTAC_w Cuts at: 0 4370 4835 4988 5364 Size: 4370 465 153 376 Fragments arranged by size: 4370 465 376 153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 739 771 1365 1397 5364 Size: 739 32 594 32 3967 Fragments arranged by size: 3967 739 594 32 32 Tth111I GACn'n_nGTC Cuts at: 0 620 5364 Size: 620 4744 XbaI T'CTAG_A Cuts at: 0 198 5364 Size: 198 5166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1044 1161 3501 3978 4452 4643 5193 5364 Size: 1044 117 2340 477 474 191 550 171 Fragments arranged by size: 2340 1044 550 477 474 191 171 117 XhoI C'TCGA_G Cuts at: 0 3104 5364 Size: 3104 2260 XmaI C'CCGG_G Cuts at: 0 502 3799 5364 Size: 502 3297 1565 Fragments arranged by size: 3297 1565 502 XmnI GAAnn'nnTTC Cuts at: 0 2537 5364 Size: 2537 2827 ZraI GAC'GTC Cuts at: 0 2747 5163 5364 Size: 2747 2416 201 Fragments arranged by size: 2747 2416 201 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SapI SbfI ScaI SfcI SfoI SmaI SmlI SnaBI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AgeI AhdI AloI AscI AsiSI AvrII BciVI BclI BfrBI BglI BmtI BsaBI BsiWI BspEI BspHI BstAPI BstBI BstZ17I ClaI DraI EcoICRI FseI FspI FspAI HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PsrI PvuI RsrII SacI SacII SalI SanDI SexAI SfiI SgfI SgrAI SpeI SrfI SspI SwaI