Restriction map for pF1KE2518
##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AgeI        ApaI        AscI        
Asp718I     AspI        AviII       AvrII       BalI        
BbrPI       BbvCI       BglI        BlnI        BsaBI       
BsgI        BsiWI       BsmBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrGI       BssHII      ClaI        
CpoI        DraIII      EagI        EclXI       Eco52I      
EcoRV       FseI        FspI        HpaI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        NaeI        NgoMIV      NotI        
NruI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PvuI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SgrAI       SmaI        SnaBI       SphI        SspBI       
Tth111I     XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccI          468  GTATAC               GT^[AC][GT]AC
AccII        2471  CGCG                 CG^CG
AccIII       1938  TCCGGA               T^CCGGA
AclI         1329  AACGTT               AA^CGTT
AflII        1668  CTTAAG               C^TTAAG
AhdI         3074  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4246  GTGCAC               G^TGCAC
AocI          821  CCTCAGG              CC^T[ACGT]AGG
Aor51HI        98  AGCGCT               AGC^GCT
ApaLI        4246  GTGCAC               G^TGCAC
AspEI        3074  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2027  CTCGAG               C^[CT]CG[GA]G
BamHI        3402  GGATCC               G^GATCC
BciVI        1199  GTATCC               GTATCC(6/5)
BclI         2594  TGATCA               T^GATCA
BfrI         1668  CTTAAG               C^TTAAG
BlpI          754  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      754  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2644  TACGTG               [CT]AC^GT[GA]
BseAI        1938  TCCGGA               T^CCGGA
BsiEI         559  CGGTCG               CG[GA][CT]^CG
BsoBI        2027  CTCGAG               C^[CT]CG[GA]G
BspEI        1938  TCCGGA               T^CCGGA
BsrFI        1108  GCCGGT               [GA]^CCGG[CT]
Bst1107I      468  GTATAC               GTA^TAC
BstBI        3791  TTCGAA               TT^CGAA
BstUI        2471  CGCG                 CG^CG
BstXI        3392  CCAGCATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       468  GTATAC               GTA^TAC
Bsu36I        821  CCTCAGG              CC^T[ACGT]AGG
CelII         754  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1108  GCCGGT               [GA]^CCGG[CT]
DraII        1318  GGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI          839  GACTTAACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3074  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1744  GAAGAG               CTCTTC(1/4)
Eco47III       98  AGCGCT               AGC^GCT
Eco81I        821  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2164  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1318  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      3416  ATGCAT               ATGCA^T
FauI          669  CCCGC                CCCGC(4/6)
FbaI         2594  TGATCA               T^GATCA
HgaI         4009  GCGTC                GACGC(5/10)
HincII       3952  GTCAAC               GT[CT]^[GA]AC
HindII       3952  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1744  GAAGAG               CTCTTC(1/4)
MroI         1938  TCCGGA               T^CCGGA
MvnI         2471  CGCG                 CG^CG
NheI         2062  GCTAGC               G^CTAGC
NsiI         3416  ATGCAT               ATGCA^T
NspV         3791  TTCGAA               TT^CGAA
PaeR7I       2027  CTCGAG               C^TCGAG
PflMI        3878  CCAATTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1318  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         880  GACCGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1329  AACGTT               AA^CGTT
PvuII         926  CAGCTG               CAG^CTG
SbfI         1169  CCTGCAGG             CCTGCA^GG
SfuI         3791  TTCGAA               TT^CGAA
Sse8387I     1169  CCTGCAGG             CCTGCA^GG
StuI         1672  AGGCCT               AGG^CCT
SwaI          210  ATTTAAAT             ATTT^AAAT
Van91I       3878  CCAATTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2027  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI         1951  GGCGCC               G[GA]^CG[CT]C
             4264  GGCGCC              
AseI         2103  ATTAAT               AT^TAAT
             2299  ATTAAT              
AsnI         2103  ATTAAT               AT^TAAT
             2299  ATTAAT              
BbeI         1951  GGCGCC               GGCGC^C
             4264  GGCGCC              
BcgI         2473  CGAGCGTTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1184  GCAGTGACCTCG        
BglII         419  AGATCT               A^GATCT
             4326  AGATCT              
BpmI          447  CTCCAG               CTGGAG(16/14)
             3269  CTCCAG              
BsaHI        1951  GGCGCC               G[GA]^CG[CT]C
             4264  GGCGCC              
BsaI         2952  GGTCTC               GGTCTC(1/5)
             3942  GGTCTC              
BsaWI        1938  TCCGGA               [AT]^CCGG[AT]
             3939  TCCGGT              
BseRI        2375  CTCCTC               GAGGAG(10/8)
             3077  GAGGAG              
BssSI         546  CACGAG               CACGAG(-5/-1)
             4330  CTCGTG              
BstAPI        496  GCAATCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2566  GCACAGTCTGC         
DraI          211  TTTAAA               TTT^AAA
             1038  TTTAAA              
DsaI         2459  CCATGG               C^C[GA][CT]GG
             3723  CCATGG              
EaeI         1099  TGGCCG               [CT]^GGCC[GA]
             4163  TGGCCG              
EcoRI        1216  GAATTC               G^AATTC
             1767  GAATTC              
Hin1I        1951  GGCGCC               G[GA]^CG[CT]C
             4264  GGCGCC              
KasI         1951  GGCGCC               G^GCGCC
             4264  GGCGCC              
NarI         1951  GGCGCC               GG^CGCC
             4264  GGCGCC              
NcoI         2459  CCATGG               C^CATGG
             3723  CCATGG              
NspI         3565  GCATGT               [GA]CATG^[CT]
             4005  ACATGC              
PshBI        2103  ATTAAT               AT^TAAT
             2299  ATTAAT              
SacI          757  GAGCTC               GAGCT^C
             2042  GAGCTC              
SfoI         1951  GGCGCC               GGC^GCC
             4264  GGCGCC              
SpeI         1654  ACTAGT               A^CTAGT
             4312  ACTAGT              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EcoO65I     EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeI        NdeII       NlaIII      NlaIV       
PleI        PstI        RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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