(Circular) (Six-base) MAPSORT of: pF1KE2462.seq Check: 4323 from: 1 to: 3467 pF1KE2462 3467 bp With 182 enzymes: SgfI * March 1, 2013 10:20 .. AccI GT'mk_AC Cuts at: 505 505 Size: 3467 Acc65I G'GTAC_C Cuts at: 483 483 Size: 3467 AclI AA'CG_TT Cuts at: 3307 3307 Size: 3467 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 226 341 1292 1724 2471 2739 226 Size: 115 951 432 747 268 954 Fragments arranged by size: 954 951 747 432 268 115 AflIII A'CryG_T Cuts at: 819 1923 2900 819 Size: 1104 977 1386 Fragments arranged by size: 1386 1104 977 AgeI A'CCGG_T Cuts at: 1855 1855 Size: 3467 AleI CACnn'nnGTG Cuts at: 322 322 Size: 3467 AlwNI CAG_nnn'CTG Cuts at: 138 141 772 2339 138 Size: 3 631 1567 1266 Fragments arranged by size: 1567 1266 631 3 ApaLI G'TGCA_C Cuts at: 2237 2237 Size: 3467 ApoI r'AATT_y Cuts at: 268 471 2820 3076 268 Size: 203 2349 256 659 Fragments arranged by size: 2349 659 256 203 AseI AT'TA_AT Cuts at: 20 20 Size: 3467 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3467 AvaI C'yCGr_G Cuts at: 487 487 Size: 3467 BamHI G'GATC_C Cuts at: 298 492 298 Size: 194 3273 BanI G'GyrC_C Cuts at: 483 897 1145 1180 483 Size: 414 248 35 2770 Fragments arranged by size: 2770 414 248 35 BanII G_rGCy'C Cuts at: 481 1511 481 Size: 1030 2437 BbeI G_GCGC'C Cuts at: 901 1149 901 Size: 248 3219 BciVI GTATCCnnnnn_n' Cuts at: 1365 2126 3467 1365 Size: 761 1341 1365 Fragments arranged by size: 1365 1341 761 BclI T'GATC_A Cuts at: 987 987 Size: 3467 BglII A'GATC_T Cuts at: 982 3019 982 Size: 2037 1430 BlpI GC'TnA_GC Cuts at: 579 579 Size: 3467 Bme1580I G_kGCm'C Cuts at: 1092 1185 2241 1092 Size: 93 1056 2318 Fragments arranged by size: 2318 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 679 760 1095 3239 679 Size: 81 335 2144 907 Fragments arranged by size: 2144 907 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1834 1834 Size: 3467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 343 343 Size: 3467 Bpu10I CC'TnA_GC Cuts at: 227 227 Size: 3467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 643 1522 2014 2312 643 Size: 879 492 298 1798 Fragments arranged by size: 1798 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 328 3171 328 Size: 2843 624 BsaAI yAC'GTr Cuts at: 820 820 Size: 3467 BsaBI GATnn'nnATC Cuts at: 6 352 981 3018 6 Size: 346 629 2037 455 Fragments arranged by size: 2037 629 455 346 BsaHI Gr'CG_yC Cuts at: 898 1146 1848 3344 898 Size: 248 702 1496 1021 Fragments arranged by size: 1496 1021 702 248 BsaWI w'CCGG_w Cuts at: 197 656 1177 1855 2129 2276 2687 197 Size: 459 521 678 274 147 411 977 Fragments arranged by size: 977 678 521 459 411 274 147 BseYI C'CCAG_C Cuts at: 892 1650 2227 2723 892 Size: 758 577 496 1636 Fragments arranged by size: 1636 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1009 1055 2263 104 Size: 905 46 1208 1308 Fragments arranged by size: 1308 1208 905 46 BsiHKAI G_wGCw'C Cuts at: 481 1259 1449 2241 481 Size: 778 190 792 1707 Fragments arranged by size: 1707 792 778 190 Bsp1286I G_dGCh'C Cuts at: 481 1092 1185 1259 1449 1511 2241 481 Size: 611 93 74 190 62 730 1707 Fragments arranged by size: 1707 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2687 2687 Size: 3467 BspHI T'CATG_A Cuts at: 2643 2643 Size: 3467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 517 731 1033 1414 517 Size: 214 302 381 2570 Fragments arranged by size: 2570 381 302 214 BsrBI CCG'CTC Cuts at: 50 1759 1813 3297 3462 50 Size: 1709 54 1484 165 55 Fragments arranged by size: 1709 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1379 1379 Size: 3467 BsrFI r'CCGG_y Cuts at: 1465 1646 1855 1465 Size: 181 209 3077 Fragments arranged by size: 3077 209 181 BssSI C'ACGA_G Cuts at: 1738 2096 1738 Size: 358 3109 BstAPI GCAn_nnn'nTGC Cuts at: 141 844 141 Size: 703 2764 BstBI TT'CG_AA Cuts at: 1828 1828 Size: 3467 BstYI r'GATC_y Cuts at: 1 298 492 959 982 1317 1563 2575 2661 Size: 297 194 467 23 335 246 1012 86 Cuts at: 2661 2673 3019 1 Size: 12 346 449 Fragments arranged by size: 1012 467 449 346 335 297 246 194 86 23 12 BtgI C'CryG_G Cuts at: 107 303 107 Size: 196 3271 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1417 1598 1761 1417 Size: 181 163 3123 Fragments arranged by size: 3123 181 163 BtsI GCAGTG_nn' Cuts at: 710 710 Size: 3467 DraI TTT'AAA Cuts at: 466 466 Size: 3467 DrdI GACnn_nn'nnGTC Cuts at: 1173 2031 1173 Size: 858 2609 EaeI y'GGCC_r Cuts at: 427 1052 1226 1617 1644 2989 427 Size: 625 174 391 27 1345 905 Fragments arranged by size: 1345 905 625 391 174 27 EagI C'GGCC_G Cuts at: 1052 1052 Size: 3467 EarI CTCTTCn'nnn_ Cuts at: 1490 1700 1490 Size: 210 3257 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1675 1985 2131 3277 1675 Size: 310 146 1146 1865 Fragments arranged by size: 1865 1146 310 146 EcoICRI GAG'CTC Cuts at: 479 479 Size: 3467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 226 341 343 1292 1724 2471 2739 226 Size: 115 2 949 432 747 268 954 Fragments arranged by size: 954 949 747 432 268 115 2 EcoO109I rG'GnC_Cy Cuts at: 606 606 Size: 3467 EcoRI G'AATT_C Cuts at: 268 471 268 Size: 203 3264 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1486 1486 Size: 3467 FspI TGC'GCA Cuts at: 1248 1248 Size: 3467 HaeII r_GCGC'y Cuts at: 901 1149 2171 3150 901 Size: 248 1022 979 1218 Fragments arranged by size: 1218 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 157 189 1309 1339 1341 1371 157 Size: 32 1120 30 2 30 2253 Fragments arranged by size: 2253 1120 32 30 30 2 HincII GTy'rAC Cuts at: 506 506 Size: 3467 KasI G'GCGC_C Cuts at: 897 1145 897 Size: 248 3219 KpnI G_GTAC'C Cuts at: 487 487 Size: 3467 MluI A'CGCG_T Cuts at: 2900 2900 Size: 3467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2138 2322 2138 Size: 184 3283 MscI TGG'CCA Cuts at: 1228 1228 Size: 3467 MslI CAynn'nnrTG Cuts at: 322 1456 1583 322 Size: 1134 127 2206 Fragments arranged by size: 2206 1134 127 MspA1I CmG'CkG Cuts at: 138 578 892 1252 2265 2510 138 Size: 440 314 360 1013 245 1095 Fragments arranged by size: 1095 1013 440 360 314 245 NaeI GCC'GGC Cuts at: 1648 1648 Size: 3467 NarI GG'CG_CC Cuts at: 898 1146 898 Size: 248 3219 NcoI C'CATG_G Cuts at: 107 107 Size: 3467 NgoMIV G'CCGG_C Cuts at: 1646 1646 Size: 3467 NspI r_CATG'y Cuts at: 520 1020 1927 520 Size: 500 907 2060 Fragments arranged by size: 2060 907 500 PciI A'CATG_T Cuts at: 1923 1923 Size: 3467 PmeI GTTT'AAAC Cuts at: 466 466 Size: 3467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2606 2638 2606 Size: 32 3435 PstI C_TGCA'G Cuts at: 146 257 514 1199 146 Size: 111 257 685 2414 Fragments arranged by size: 2414 685 257 111 PvuI CG_AT'CG Cuts at: 104 104 Size: 3467 PvuII CAG'CTG Cuts at: 138 892 1252 138 Size: 754 360 2353 Fragments arranged by size: 2353 754 360 RsrII CG'GwC_CG Cuts at: 1662 1662 Size: 3467 SacI G_AGCT'C Cuts at: 481 481 Size: 3467 SalI G'TCGA_C Cuts at: 504 504 Size: 3467 SapI GCTCTTCn'nnn_ Cuts at: 1490 1700 1490 Size: 210 3257 SbfI CC_TGCA'GG Cuts at: 514 514 Size: 3467 ScaI AGT'ACT Cuts at: 2984 2984 Size: 3467 SfcI C'TryA_G Cuts at: 33 142 253 510 1195 2188 2379 33 Size: 109 111 257 685 993 191 1121 Fragments arranged by size: 1121 993 685 257 191 111 109 SfoI GGC'GCC Cuts at: 899 1147 899 Size: 248 3219 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3467 SmaI CCC'GGG Cuts at: 489 489 Size: 3467 SmlI C'TyrA_G Cuts at: 622 1537 2029 2291 622 Size: 915 492 262 1798 Fragments arranged by size: 1798 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3467 SphI G_CATG'C Cuts at: 520 1020 520 Size: 500 2967 StyI C'CwwG_G Cuts at: 107 278 601 955 107 Size: 171 323 354 2619 Fragments arranged by size: 2619 354 323 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1050 1183 1642 1851 1050 Size: 133 459 209 2666 Fragments arranged by size: 2666 459 209 133 TatI w'GTAC_w Cuts at: 2982 2982 Size: 3467 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2984 3016 2984 Size: 32 3435 Tth111I GACn'n_nGTC Cuts at: 1264 1264 Size: 3467 XbaI T'CTAG_A Cuts at: 498 498 Size: 3467 XcmI CCAnnnn_n'nnnnTGG Cuts at: 962 962 Size: 3467 XmaI C'CCGG_G Cuts at: 487 487 Size: 3467 XmnI GAAnn'nnTTC Cuts at: 268 268 Size: 3467 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI