Restriction map for pF1KE1875
 (Linear) (Six-base) MAPSORT of: KIEE1875.seq  Check: 1052  from: 1  to: 1194



KIEE1875.orf

 With 182 enzymes: SgfI * 

                        July 27, 2017 10:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     733    1194 
   Size:        733     461
 
AccI GT'mk_AC

Cuts at:      0    1159    1194 
   Size:       1159      35
 
AlwNI CAG_nnn'CTG

Cuts at:      0     512     618    1134    1194 
   Size:        512     106     516      60

  Fragments arranged by size: 

                 516     512     106      60
 
ApaI G_GGCC'C

Cuts at:      0     246     418    1194 
   Size:        246     172     776

  Fragments arranged by size: 

                 776     246     172
 
AvaI C'yCGr_G

Cuts at:      0     285    1194 
   Size:        285     909
 
BanI G'GyrC_C

Cuts at:      0     265    1194 
   Size:        265     929
 
BanII G_rGCy'C

Cuts at:      0     230     246     418     458    1194 
   Size:        230      16     172      40     736

  Fragments arranged by size: 

                 736     230     172      40      16
 
BbeI G_GCGC'C

Cuts at:      0     269    1194 
   Size:        269     925
 
BglI GCCn_nnn'nGGC

Cuts at:      0     290    1194 
   Size:        290     904
 
BlpI GC'TnA_GC

Cuts at:      0     674    1194 
   Size:        674     520
 
Bme1580I G_kGCm'C

Cuts at:      0     246     359     418    1194 
   Size:        246     113      59     776

  Fragments arranged by size: 

                 776     246     113      59
 
BmgBI CAC'GTC

Cuts at:      0     744    1194 
   Size:        744     450
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     298     346     592    1194 
   Size:        298      48     246     602

  Fragments arranged by size: 

                 602     298     246      48
 
BmtI G_CTAG'C

Cuts at:      0     335    1194 
   Size:        335     859
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     472    1194 
   Size:        282     190     722

  Fragments arranged by size: 

                 722     282     190
 
Bpu10I CC'TnA_GC

Cuts at:      0      22     139    1194 
   Size:         22     117    1055

  Fragments arranged by size: 

                1055     117      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556    1014    1194 
   Size:        556     458     180

  Fragments arranged by size: 

                 556     458     180
 
BsaHI Gr'CG_yC

Cuts at:      0     104     266    1194 
   Size:        104     162     928

  Fragments arranged by size: 

                 928     162     104
 
BsaWI w'CCGG_w

Cuts at:      0     230     235    1194 
   Size:        230       5     959

  Fragments arranged by size: 

                 959     230       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     248     261     409     512     670     947    1194 
   Size:        248      13     148     103     158     277     247

  Fragments arranged by size: 

                 277     248     247     158     148     103      13
 
BseYI C'CCAG_C

Cuts at:      0     275     926    1194 
   Size:        275     651     268

  Fragments arranged by size: 

                 651     275     268
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    1194 
   Size:         79    1115
 
BsiEI CG_ry'CG

Cuts at:      0     250     495    1194 
   Size:        250     245     699

  Fragments arranged by size: 

                 699     250     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     230     458     569    1194 
   Size:        230     228     111     625

  Fragments arranged by size: 

                 625     230     228     111
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     522    1194 
   Size:        522     672
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     246     359     418     458     569    1194 
   Size:        230      16     113      59      40     111     625

  Fragments arranged by size: 

                 625     230     113     111      59      40      16
 
BspEI T'CCGG_A

Cuts at:      0     230    1194 
   Size:        230     964
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     733    1194 
   Size:        733     461
 
BsrBI CCG'CTC

Cuts at:      0      18      26    1194 
   Size:         18       8    1168

  Fragments arranged by size: 

                1168      18       8
 
BsrDI GCAATG_nn'

Cuts at:      0     821    1194 
   Size:        821     373
 
BsrFI r'CCGG_y

Cuts at:      0     557    1194 
   Size:        557     637
 
BsrGI T'GTAC_A

Cuts at:      0    1140    1194 
   Size:       1140      54
 
BssSI C'ACGA_G

Cuts at:      0      76     457    1194 
   Size:         76     381     737

  Fragments arranged by size: 

                 737     381      76
 
BstZ17I GTA'TAC

Cuts at:      0    1160    1194 
   Size:       1160      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     392    1194 
   Size:        392     802
 
BtgI C'CryG_G

Cuts at:      0     796    1194 
   Size:        796     398
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      52    1194 
   Size:         52    1142
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     980    1015    1194 
   Size:        980      35     179

  Fragments arranged by size: 

                 980     179      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     531    1194 
   Size:        531     663
 
EaeI y'GGCC_r

Cuts at:      0      80     129     247     492     533    1194 
   Size:         80      49     118     245      41     661

  Fragments arranged by size: 

                 661     245     118      80      49      41
 
EagI C'GGCC_G

Cuts at:      0     247     492    1194 
   Size:        247     245     702

  Fragments arranged by size: 

                 702     247     245
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1012    1194 
   Size:       1012     182
 
EcoICRI GAG'CTC

Cuts at:      0     228     456    1194 
   Size:        228     228     738

  Fragments arranged by size: 

                 738     228     228
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     472    1194 
   Size:        282     190     722

  Fragments arranged by size: 

                 722     282     190
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     260    1194 
   Size:        260     934
 
EcoO109I rG'GnC_Cy

Cuts at:      0     242     415    1194 
   Size:        242     173     779

  Fragments arranged by size: 

                 779     242     173
 
HaeII r_GCGC'y

Cuts at:      0      35     269     612    1194 
   Size:         35     234     343     582

  Fragments arranged by size: 

                 582     343     234      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     502     534    1194 
   Size:        502      32     660

  Fragments arranged by size: 

                 660     502      32
 
KasI G'GCGC_C

Cuts at:      0     265    1194 
   Size:        265     929
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     141    1194 
   Size:        141    1053
 
MscI TGG'CCA

Cuts at:      0     535    1194 
   Size:        535     659
 
MslI CAynn'nnrTG

Cuts at:      0     442     757    1194 
   Size:        442     315     437

  Fragments arranged by size: 

                 442     437     315
 
MspA1I CmG'CkG

Cuts at:      0      60     177     405     618    1194 
   Size:         60     117     228     213     576

  Fragments arranged by size: 

                 576     228     213     117      60
 
NarI GG'CG_CC

Cuts at:      0     266    1194 
   Size:        266     928
 
NheI G'CTAG_C

Cuts at:      0     331    1194 
   Size:        331     863
 
NotI GC'GGCC_GC

Cuts at:      0     492    1194 
   Size:        492     702
 
NspI r_CATG'y

Cuts at:      0     441     978    1194 
   Size:        441     537     216

  Fragments arranged by size: 

                 537     441     216
 
PasI CC'CwG_GG

Cuts at:      0     506    1194 
   Size:        506     688
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     618    1134    1194 
   Size:        618     516      60

  Fragments arranged by size: 

                 618     516      60
 
PshAI GACnn'nnGTC

Cuts at:      0     346    1194 
   Size:        346     848
 
PspOMI G'GGCC_C

Cuts at:      0     242     414    1194 
   Size:        242     172     780

  Fragments arranged by size: 

                 780     242     172
 
PstI C_TGCA'G

Cuts at:      0     182     608    1194 
   Size:        182     426     586

  Fragments arranged by size: 

                 586     426     182
 
PvuII CAG'CTG

Cuts at:      0     618    1194 
   Size:        618     576
 
RsrII CG'GwC_CG

Cuts at:      0     233    1194 
   Size:        233     961
 
SacI G_AGCT'C

Cuts at:      0     230     458    1194 
   Size:        230     228     736

  Fragments arranged by size: 

                 736     230     228
 
ScaI AGT'ACT

Cuts at:      0     577    1194 
   Size:        577     617
 
SexAI A'CCwGG_T

Cuts at:      0    1127    1194 
   Size:       1127      67
 
SfcI C'TryA_G

Cuts at:      0      55     178     604    1194 
   Size:         55     123     426     590

  Fragments arranged by size: 

                 590     426     123      55
 
SfoI GGC'GCC

Cuts at:      0     267    1194 
   Size:        267     927
 
SmaI CCC'GGG

Cuts at:      0     287    1194 
   Size:        287     907
 
SmlI C'TyrA_G

Cuts at:      0     571    1029    1194 
   Size:        571     458     165

  Fragments arranged by size: 

                 571     458     165
 
StyI C'CwwG_G

Cuts at:      0      99    1194 
   Size:         99    1095
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     336     611    1085    1194 
   Size:        336     275     474     109

  Fragments arranged by size: 

                 474     336     275     109
 
TatI w'GTAC_w

Cuts at:      0     575    1140    1194 
   Size:        575     565      54

  Fragments arranged by size: 

                 575     565      54
 
Tth111I GACn'n_nGTC

Cuts at:      0     514    1194 
   Size:        514     680
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     306    1194 
   Size:        306     888
 
XmaI C'CCGG_G

Cuts at:      0     285    1194 
   Size:        285     909
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI   DraIII     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NheI     NotI
     NspI     PasI    PflMI    PshAI   PspOMI     PstI    PvuII    RsrII
     SacI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     StyI
    TaqII     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BplI     BsaI    BsaAI    BsaBI    BsaXI
    BsiWI     BsmI    BspHI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtsI     ClaI     DraI     DrdI     EarI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
    PspXI     PsrI     PvuI    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI     XbaI     XhoI     XmnI     ZraI
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