Restriction map for pF1KE0947
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbrPI       BbvCI       
BcgI        BfaI        BlnI        BsaAI       BsaBI       
BsaI        BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          93  CGCG                 CG^CG
AcsI          596  AAATTT               [GA]^AATT[CT]
AfaI          681  GTAC                 GT^AC
AflII        1119  CTTAAG               C^TTAAG
AflIII        731  ACATGT               A^C[GA][CT]GT
AlwNI         937  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          691  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        42  AGCGCT               AGC^GCT
ApaI          103  GGGCCC               GGGCC^C
ApoI          596  AAATTT               [GA]^AATT[CT]
AspHI         430  GAGCTC               G[AT]GC[AT]^C
BanI           10  GGCGCC               G^G[CT][GA]CC
BbeI           10  GGCGCC               GGCGC^C
BciVI         134  GGATAC               GTATCC(6/5)
BfrI         1119  CTTAAG               C^TTAAG
BglI          118  GCCCAGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         374  AGATCT               A^GATCT
BlpI          324  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      324  GCTGAGC              GC^T[ACGT]AGC
BsaWI         421  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       430  GAGCTC               G[AT]GC[AT]^C
BsmAI         382  GAGAC                GTCTC(1/5)
BspLU11I      731  ACATGT               A^CATGT
BsrBI         469  GAGCGG               CCGCTC(-3/-3)
BsrDI         447  CATTGC               GCAATG(2/0)
BsrFI          79  GCCGGC               [GA]^CCGG[CT]
BstAPI        522  GCATGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          93  CGCG                 CG^CG
BstXI         719  CCAGTGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        691  CCTGAGG              CC^T[ACGT]AGG
CelII         324  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         79  GCCGGC               [GA]^CCGG[CT]
DraIII        662  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          166  GACCCCCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          965  CCATGG               C^C[GA][CT]GG
EarI          586  GAAGAG               CTCTTC(1/4)
Eco47III       42  AGCGCT               AGC^GCT
Eco81I        691  CCTGAGG              CC^T[ACGT]AGG
KasI           10  GGCGCC               G^GCGCC
Ksp632I       586  GAAGAG               CTCTTC(1/4)
MspA1I        929  CAGCTG               C[AC]G^C[GT]G
MvnI           93  CGCG                 CG^CG
NaeI           79  GCCGGC               GCC^GGC
NarI           10  GGCGCC               GG^CGCC
NcoI          965  CCATGG               C^CATGG
NgoMIV         79  GCCGGC               G^CCGGC
PvuII         929  CAGCTG               CAG^CTG
RsaI          681  GTAC                 GT^AC
SacI          430  GAGCTC               GAGCT^C
SfaNI         475  GATGC                GCATC(5/9)
SfoI           10  GGCGCC               GGC^GCC
SmlI         1119  CTTAAG               C^T[CT][GA]AG
SphI          522  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI           10  GGCGCC               G[GA]^CG[CT]C
               82  GGCGTC              
BalI          856  TGGCCA               TGG^CCA
              933  TGGCCA              
BamHI         457  GGATCC               G^GATCC
              485  GGATCC              
BbvI          528  GCTGC                GCAGC(8/12)
              970  GCTGC               
BclI          749  TGATCA               T^GATCA
              959  TGATCA              
BpmI          490  CTCCAG               CTGGAG(16/14)
              994  CTCCAG              
BsaHI          10  GGCGCC               G[GA]^CG[CT]C
               82  GGCGTC              
BsgI         1058  CTGCAC               GTGCAG(16/14)
              722  GTGCAG              
BsrI          647  ACTGG                ACTGG(1/-1)
              719  CCAGT               
EaeI          856  TGGCCA               [CT]^GGCC[GA]
              933  TGGCCA              
EcoT14I       853  CCTTGG               C^C[AT][AT]GG
              965  CCATGG              
FbaI          749  TGATCA               T^GATCA
              959  TGATCA              
HaeII          10  GGCGCC               [GA]GCGC^[CT]
               42  AGCGCT              
HgaI           83  GCGTC                GACGC(5/10)
              329  GCGTC               
Hin1I          10  GGCGCC               G[GA]^CG[CT]C
               82  GGCGTC              
MluNI         856  TGGCCA               TGG^CCA
              933  TGGCCA              
MscI          856  TGGCCA               TGG^CCA
              933  TGGCCA              
MseI          601  TTAA                 T^TAA
             1120  TTAA                
MslI          402  CATGGTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              577  CATCCAGGTG          
NdeI          755  CATATG               CA^TATG
             1099  CATATG              
PflMI         363  CCAGCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              936  CCAGGCCCTGG         
PpuMI         173  GGGTCCT              [GA]G^G[AT]CC[CT]
              670  GGGACCC             
PstI          529  CTGCAG               CTGCA^G
              971  CTGCAG              
SfcI          529  CTGCAG               C^T[GA][CT]AG
              971  CTGCAG              
SmaI           30  CCCGGG               CCC^GGG
              170  CCCGGG              
StyI          853  CCTTGG               C^C[AT][AT]GG
              965  CCATGG              
Tru9I         601  TTAA                 T^TAA
             1120  TTAA                
TseI          528  GCTGC                G^C[AT]GC
              970  GCTGC               
Tsp45I        644  GTGAC                ^GT[GC]AC
             1074  GTCAC               
Van91I        363  CCAGCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              936  CCAGGCCCTGG         
XcmI           14  CCAGCAGGAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
               40  CCAGCGCTGGCTTGG     
XmaCI          30  CCCGGG               C^CCGGG
              170  CCCGGG              
XmaI           30  CCCGGG               C^CCGGG
              170  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BanII       BbsI        BcnI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        Sau3AI      Sau96I      
ScrFI       TaqI        TfiI        Tsp509I     TspRI       
TthHB8I     XhoII       
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