Restriction map for pF1KE0947
 (Linear) (Six-base) MAPSORT of: KIEE0947.seq  Check: 4409  from: 1  to: 1155



KIEE0947.orf

 With 182 enzymes: SgfI * 

                        November 18, 2016 17:23  ..

AfeI AGC'GCT

Cuts at:      0      44    1155 
   Size:         44    1111
 
AflII C'TTAA_G

Cuts at:      0    1119    1155 
   Size:       1119      36
 
AflIII A'CryG_T

Cuts at:      0     731    1155 
   Size:        731     424
 
AlwNI CAG_nnn'CTG

Cuts at:      0     942    1155 
   Size:        942     213
 
ApaI G_GGCC'C

Cuts at:      0     107    1155 
   Size:        107    1048
 
ApoI r'AATT_y

Cuts at:      0     596    1155 
   Size:        596     559
 
AvaI C'yCGr_G

Cuts at:      0      30     106     170    1155 
   Size:         30      76      64     985

  Fragments arranged by size: 

                 985      76      64      30
 
BamHI G'GATC_C

Cuts at:      0     457     485    1155 
   Size:        457      28     670

  Fragments arranged by size: 

                 670     457      28
 
BanI G'GyrC_C

Cuts at:      0      10    1155 
   Size:         10    1145
 
BanII G_rGCy'C

Cuts at:      0     107     434     741    1155 
   Size:        107     327     307     414

  Fragments arranged by size: 

                 414     327     307     107
 
BbeI G_GCGC'C

Cuts at:      0      14    1155 
   Size:         14    1141
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     253     664     971    1058    1155 
   Size:        253     411     307      87      97

  Fragments arranged by size: 

                 411     307     253      97      87
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     128    1155 
   Size:        128    1027
 
BclI T'GATC_A

Cuts at:      0     749     959    1155 
   Size:        749     210     196

  Fragments arranged by size: 

                 749     210     196
 
BglI GCCn_nnn'nGGC

Cuts at:      0     124    1155 
   Size:        124    1031
 
BglII A'GATC_T

Cuts at:      0     374    1155 
   Size:        374     781
 
BlpI GC'TnA_GC

Cuts at:      0     325    1155 
   Size:        325     830
 
Bme1580I G_kGCm'C

Cuts at:      0     107    1155 
   Size:        107    1048
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     448    1049    1155 
   Size:        448     601     106

  Fragments arranged by size: 

                 601     448     106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     979    1155 
   Size:        475     504     176

  Fragments arranged by size: 

                 504     475     176
 
Bpu10I CC'TnA_GC

Cuts at:      0     452     703    1155 
   Size:        452     251     452

  Fragments arranged by size: 

                 452     452     251
 
BsaHI Gr'CG_yC

Cuts at:      0      11      83    1155 
   Size:         11      72    1072

  Fragments arranged by size: 

                1072      72      11
 
BsaWI w'CCGG_w

Cuts at:      0     421    1155 
   Size:        421     734
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     743    1043    1155 
   Size:        743     300     112

  Fragments arranged by size: 

                 743     300     112
 
BsiHKAI G_wGCw'C

Cuts at:      0     434    1155 
   Size:        434     721
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     434     741    1155 
   Size:        107     327     307     414

  Fragments arranged by size: 

                 414     327     307     107
 
BsrBI CCG'CTC

Cuts at:      0     471    1155 
   Size:        471     684
 
BsrDI GCAATG_nn'

Cuts at:      0     446    1155 
   Size:        446     709
 
BsrFI r'CCGG_y

Cuts at:      0      79    1155 
   Size:         79    1076
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     528    1155 
   Size:        528     627
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     726    1155 
   Size:        726     429
 
BstYI r'GATC_y

Cuts at:      0     374     457     485    1054    1155 
   Size:        374      83      28     569     101

  Fragments arranged by size: 

                 569     374     101      83      28
 
Bsu36I CC'TnA_GG

Cuts at:      0     692    1155 
   Size:        692     463
 
BtgI C'CryG_G

Cuts at:      0     965    1155 
   Size:        965     190
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     904    1155 
   Size:        904     251
 
BtsI GCAGTG_nn'

Cuts at:      0     731    1155 
   Size:        731     424
 
DraIII CAC_nnn'GTG

Cuts at:      0     667    1155 
   Size:        667     488
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     172    1155 
   Size:        172     983
 
EaeI y'GGCC_r

Cuts at:      0     856     933    1155 
   Size:        856      77     222

  Fragments arranged by size: 

                 856     222      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     581    1155 
   Size:        581     574
 
EcoICRI GAG'CTC

Cuts at:      0     432    1155 
   Size:        432     723
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     979    1155 
   Size:        475     504     176

  Fragments arranged by size: 

                 504     475     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0      97     174     671     939    1028    1155 
   Size:         97      77     497     268      89     127

  Fragments arranged by size: 

                 497     268     127      97      89      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     728    1134    1155 
   Size:        728     406      21

  Fragments arranged by size: 

                 728     406      21
 
HaeII r_GCGC'y

Cuts at:      0      14      46    1155 
   Size:         14      32    1109

  Fragments arranged by size: 

                1109      32      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     176     208     375     407    1155 
   Size:        176      32     167      32     748

  Fragments arranged by size: 

                 748     176     167      32      32
 
KasI G'GCGC_C

Cuts at:      0      10    1155 
   Size:         10    1145
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      66    1155 
   Size:         66    1089
 
MscI TGG'CCA

Cuts at:      0     858     935    1155 
   Size:        858      77     220

  Fragments arranged by size: 

                 858     220      77
 
MslI CAynn'nnrTG

Cuts at:      0     406     581    1155 
   Size:        406     175     574

  Fragments arranged by size: 

                 574     406     175
 
MspA1I CmG'CkG

Cuts at:      0     931    1155 
   Size:        931     224
 
NaeI GCC'GGC

Cuts at:      0      81    1155 
   Size:         81    1074
 
NarI GG'CG_CC

Cuts at:      0      11    1155 
   Size:         11    1144
 
NcoI C'CATG_G

Cuts at:      0     965    1155 
   Size:        965     190
 
NdeI CA'TA_TG

Cuts at:      0     756    1100    1155 
   Size:        756     344      55

  Fragments arranged by size: 

                 756     344      55
 
NgoMIV G'CCGG_C

Cuts at:      0      79    1155 
   Size:         79    1076
 
NspI r_CATG'y

Cuts at:      0     526     735     927    1155 
   Size:        526     209     192     228

  Fragments arranged by size: 

                 526     228     209     192
 
PasI CC'CwG_GG

Cuts at:      0     953    1155 
   Size:        953     202
 
PciI A'CATG_T

Cuts at:      0     731    1155 
   Size:        731     424
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     369     942    1155 
   Size:        369     573     213

  Fragments arranged by size: 

                 573     369     213
 
PfoI T'CCnGG_A

Cuts at:      0     628    1050    1155 
   Size:        628     422     105

  Fragments arranged by size: 

                 628     422     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     671    1155 
   Size:        174     497     484

  Fragments arranged by size: 

                 497     484     174
 
PspOMI G'GGCC_C

Cuts at:      0     103    1155 
   Size:        103    1052
 
PstI C_TGCA'G

Cuts at:      0     533     975    1155 
   Size:        533     442     180

  Fragments arranged by size: 

                 533     442     180
 
PvuII CAG'CTG

Cuts at:      0     931    1155 
   Size:        931     224
 
SacI G_AGCT'C

Cuts at:      0     434    1155 
   Size:        434     721
 
SanDI GG'GwC_CC

Cuts at:      0     671    1155 
   Size:        671     484
 
SfcI C'TryA_G

Cuts at:      0     529     971    1155 
   Size:        529     442     184

  Fragments arranged by size: 

                 529     442     184
 
SfoI GGC'GCC

Cuts at:      0      12    1155 
   Size:         12    1143
 
SmaI CCC'GGG

Cuts at:      0      32     172    1155 
   Size:         32     140     983

  Fragments arranged by size: 

                 983     140      32
 
SmlI C'TyrA_G

Cuts at:      0    1119    1155 
   Size:       1119      36
 
SphI G_CATG'C

Cuts at:      0     526    1155 
   Size:        526     629
 
StyI C'CwwG_G

Cuts at:      0     853     965    1155 
   Size:        853     112     190

  Fragments arranged by size: 

                 853     190     112
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     945    1155 
   Size:        945     210
 
TatI w'GTAC_w

Cuts at:      0     680    1155 
   Size:        680     475
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     624     656    1155 
   Size:        624      32     499

  Fragments arranged by size: 

                 624     499      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      21      47    1155 
   Size:         21      26    1108

  Fragments arranged by size: 

                1108      26      21
 
XmaI C'CCGG_G

Cuts at:      0      30     170    1155 
   Size:         30     140     985

  Fragments arranged by size: 

                 985     140      30
 

 Enzymes that do cut:

     AfeI    AflII   AflIII    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BplI     BpmI   Bpu10I    BsaHI    BsaWI     BsgI
  BsiHKAI Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI     PstI
    PvuII     SacI    SanDI     SfcI     SfoI     SmaI     SmlI     SphI
     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmrI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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