Restriction map for pF1KE1706
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvrII       BalI        BanII       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BseRI       BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT14I     FbaI        FseI        
HgaI        HincII      HindII      HindIII     HinfI       
HpaI        HphI        KpnI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NcoI        NdeI        NheI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tsp45I      Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          532  GGCGCC               G[GA]^CG[CT]C
AlwNI         232  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       592  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         162  TGCGCA               TGC^GCA
BamHI         585  GGATCC               G^GATCC
BbeI          532  GGCGCC               GGCGC^C
BfaI          575  CTAG                 C^TAG
BmyI          258  GGGCAC               G[AGT]GC[ACT]^C
BpmI           40  CTGGAG               CTGGAG(16/14)
BsaHI         532  GGCGCC               G[GA]^CG[CT]C
BsaI          346  GGTCTC               GGTCTC(1/5)
BsiEI          97  CGGCCG               CG[GA][CT]^CG
BsmFI         315  GGGAC                GGGAC(10/14)
BsmI          406  GAATGC               GAATGC(1/-1)
Bsp1286I      258  GGGCAC               G[AGT]GC[ACT]^C
BstAPI        231  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        186  CATCC                GGATG(2/0)
DdeI            9  CTTAG                C^T[ACGT]AG
DraII         614  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          144  CCGCGG               C^C[GA][CT]GG
EagI           97  CGGCCG               C^GGCCG
EclXI          97  CGGCCG               C^GGCCG
Eco52I         97  CGGCCG               C^GGCCG
EcoO109I      614  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         295  GAATTC               G^AATTC
EcoT22I       408  ATGCAT               ATGCA^T
FokI          186  CATCC                GGATG(9/13)
FspI          162  TGCGCA               TGC^GCA
Hin1I         532  GGCGCC               G[GA]^CG[CT]C
KasI          532  GGCGCC               G^GCGCC
KspI          144  CCGCGG               CCGC^GG
MaeI          575  CTAG                 C^TAG
MseI          506  TTAA                 T^TAA
MslI          300  CATCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          197  GCCGGC               GCC^GGC
NarI          532  GGCGCC               GG^CGCC
NgoMIV        197  GCCGGC               G^CCGGC
NlaIII        472  CATG                 CATG^
NotI           96  GCGGCCGC             GC^GGCCGC
NsiI          408  ATGCAT               ATGCA^T
PpuMI         614  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         340  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          229  CTGCAG               CTGCA^G
PvuII         166  CAGCTG               CAG^CTG
SacII         144  CCGCGG               CCGC^GG
SfcI          229  CTGCAG               C^T[GA][CT]AG
SfoI          532  GGCGCC               GGC^GCC
Tru9I         506  TTAA                 T^TAA
XmnI          592  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         126  CGCG                 CG^CG
              145  CGCG                
AcsI          295  GAATTC               [GA]^AATT[CT]
              556  AAATTT              
AfaI          609  GTAC                 GT^AC
              627  GTAC                
AluI          167  AGCT                 AG^CT
              203  AGCT                
ApoI          295  GAATTC               [GA]^AATT[CT]
              556  AAATTT              
BanI          259  GGCACC               G^G[CT][GA]CC
              532  GGCGCC              
BglI           38  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              212  GCCGCACCGGC         
BsgI          178  CTGCAC               GTGCAG(16/14)
              253  GTGCAG              
BsmAI         476  GAGAC                GTCTC(1/5)
              347  GTCTC               
Bsp1407I      608  TGTACA               T^GTACA
              626  TGTACA              
BsrBI          94  GAGCGG               CCGCTC(-3/-3)
              133  GAGCGG              
BsrFI         197  GCCGGC               [GA]^CCGG[CT]
              217  ACCGGC              
BsrGI         608  TGTACA               T^GTACA
              626  TGTACA              
BstNI          39  CCTGG                CC^[AT]GG
              536  CCAGG               
BstUI         126  CGCG                 CG^CG
              145  CGCG                
BstYI         233  AGATCC               [GA]^GATC[CT]
              585  GGATCC              
Cfr10I        197  GCCGGC               [GA]^CCGG[CT]
              217  ACCGGC              
EaeI           97  CGGCCG               [CT]^GGCC[GA]
              483  TGGCCG              
EarI          280  CTCTTC               CTCTTC(1/4)
              430  GAAGAG              
EcoRII         39  CCTGG                ^CC[AT]GG
              536  CCAGG               
FauI          143  CCCGC                CCCGC(4/6)
              149  GCGGG               
HaeII         139  AGCGCC               [GA]GCGC^[CT]
              532  GGCGCC              
Ksp632I       280  CTCTTC               CTCTTC(1/4)
              430  GAAGAG              
MboII         430  GAAGA                GAAGA(8/7)
              281  TCTTC               
MflI          233  AGATCC               [GA]^GATC[CT]
              585  GGATCC              
MvaI           39  CCTGG                CC^[AT]GG
              536  CCAGG               
MvnI          126  CGCG                 CG^CG
              145  CGCG                
PflMI          54  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              523  CCAAGAGATGG         
RsaI          609  GTAC                 GT^AC
              627  GTAC                
SspBI         608  TGTACA               T^GTACA
              626  TGTACA              
Van91I         54  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              523  CCAAGAGATGG         
XhoII         233  AGATCC               [GA]^GATC[CT]
              585  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AlwI        AvaII       BbvI        BcnI        
BsaJI       BsiYI       BslI        BspMI       BsrI        
BssKI       Cac8I       CfoI        Cfr13I      DpnII       
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboI        MnlI        MspA1I      
MspI        MwoI        NciI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp509I     TspRI       
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