Restriction map for
pF1KE1706
(Circular) (Six-base) MAPSORT of: pF1KE1706.seq Check: 349 from: 1 to: 3746
pF1KE1706 3746 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 156 293 344 156
Size: 137 51 3558
Fragments arranged by size:
3558 137 51
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 345 1051 2618 345
Size: 706 1567 1473
Fragments arranged by size:
1567 1473 706
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 403 664 750 3099 3355 403
Size: 261 86 2349 256 794
Fragments arranged by size:
2349 794 261 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 693 771 693
Size: 78 3668
BanI G'GyrC_C
Cuts at: 367 640 762 1176 1424 1459 367
Size: 273 122 414 248 35 2654
Fragments arranged by size:
2654 414 273 248 122 35
BanII G_rGCy'C
Cuts at: 760 1790 760
Size: 1030 2716
BbeI G_GCGC'C
Cuts at: 644 1180 1428 644
Size: 536 248 2962
Fragments arranged by size:
2962 536 248
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BfrBI ATG'CAT
Cuts at: 518 518
Size: 3746
BglI GCCn_nnn'nGGC
Cuts at: 152 326 152
Size: 174 3572
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 370 1371 1464 2520 370
Size: 1001 93 1056 1596
Fragments arranged by size:
1596 1056 1001 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 3746
Bpu10I CC'TnA_GC
Cuts at: 117 117
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 3450 460
Size: 2990 756
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 641 1177 1425 2127 3623 641
Size: 536 248 702 1496 764
Fragments arranged by size:
1496 764 702 536 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 216 1171 1929 2506 3002 216
Size: 955 758 577 496 960
Fragments arranged by size:
960 955 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 382 271
Size: 111 3635
BsiEI CG_ry'CG
Cuts at: 104 208 1288 1334 2542 104
Size: 104 1080 46 1208 1308
Fragments arranged by size:
1308 1208 1080 104 46
BsiHKAI G_wGCw'C
Cuts at: 760 1538 1728 2520 760
Size: 778 190 792 1986
Fragments arranged by size:
1986 792 778 190
BsmI GAATG_Cn'
Cuts at: 520 520
Size: 3746
Bsp1286I G_dGCh'C
Cuts at: 370 760 1371 1464 1538 1728 1790 2520 370
Size: 390 611 93 74 190 62 730 1596
Fragments arranged by size:
1596 730 611 390 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 293 344 587 796 1010 1312 1693 156
Size: 137 51 243 209 214 302 381 2209
Fragments arranged by size:
2209 381 302 243 214 209 137 51
BsrBI CCG'CTC
Cuts at: 50 204 243 2038 2092 3576 3741 50
Size: 154 39 1795 54 1484 165 55
Fragments arranged by size:
1795 1484 165 154 55 54 39
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 305 325 1744 1925 2134 305
Size: 20 1419 181 209 1917
Fragments arranged by size:
1917 1419 209 181 20
BsrGI T'GTAC_A
Cuts at: 716 734 716
Size: 18 3728
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 345 1123 345
Size: 778 2968
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 341 693 771 1238 1261 1596 1842 2854
Size: 340 352 78 467 23 335 246 1012
Cuts at: 2854 2940 2952 3298 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 352 346 340 335 246
86 78 23 12
BtgI C'CryG_G
Cuts at: 107 252 107
Size: 145 3601
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 425 989 425
Size: 564 3182
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 205 591 1331 1505 1896 1923 3268 205
Size: 386 740 174 391 27 1345 683
Fragments arranged by size:
1345 740 683 391 386 174 27
EagI C'GGCC_G
Cuts at: 205 1331 205
Size: 1126 2620
EarI CTCTTCn'nnn_
Cuts at: 394 533 1769 1979 394
Size: 139 1236 210 2161
Fragments arranged by size:
2161 1236 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 253 1954 2264 2410 3556 253
Size: 1701 310 146 1146 443
Fragments arranged by size:
1701 1146 443 310 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1571 2003 2750 3018 169
Size: 1402 432 747 268 897
Fragments arranged by size:
1402 897 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 723 885 723
Size: 162 3584
EcoRI G'AATT_C
Cuts at: 403 750 403
Size: 347 3399
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 272 1527 272
Size: 1255 2491
HaeII r_GCGC'y
Cuts at: 251 644 1180 1428 2450 3429 251
Size: 393 536 248 1022 979 568
Fragments arranged by size:
1022 979 568 536 393 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 640 1176 1424 640
Size: 536 248 2962
Fragments arranged by size:
2962 536 248
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 412 1735 1862 412
Size: 1323 127 2296
Fragments arranged by size:
2296 1323 127
MspA1I CmG'CkG
Cuts at: 213 254 276 857 1171 1531 2544 2789 213
Size: 41 22 581 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 581 360 314 245 41 22
NaeI GCC'GGC
Cuts at: 307 1927 307
Size: 1620 2126
NarI GG'CG_CC
Cuts at: 641 1177 1425 641
Size: 536 248 2962
Fragments arranged by size:
2962 536 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 305 1925 305
Size: 1620 2126
NotI GC'GGCC_GC
Cuts at: 205 205
Size: 3746
NsiI A_TGCA'T
Cuts at: 520 520
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PflMI CCAn_nnn'nTGG
Cuts at: 168 637 168
Size: 469 3277
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PpuMI rG'GwC_Cy
Cuts at: 723 723
Size: 3746
PshAI GACnn'nnGTC
Cuts at: 452 452
Size: 3746
PstI C_TGCA'G
Cuts at: 341 793 1478 341
Size: 452 685 2609
Fragments arranged by size:
2609 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 276 1171 1531 276
Size: 895 360 2491
Fragments arranged by size:
2491 895 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SacII CC_GC'GG
Cuts at: 255 255
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 337 789 1474 2467 2658 33
Size: 304 452 685 993 191 1121
Fragments arranged by size:
1121 993 685 452 304 191
SfoI GGC'GCC
Cuts at: 642 1178 1426 642
Size: 536 248 2962
Fragments arranged by size:
2962 536 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 880 1234 107
Size: 773 354 2619
Fragments arranged by size:
2619 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 716 734 3261 716
Size: 18 2527 1201
Fragments arranged by size:
2527 1201 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1241
Size: 3746
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 704 704
Size: 3746
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NsiI NspI PciI PflMI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI
BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI PacI PasI
PfoI PmlI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI